Journal of Food Hygiene and Safety (한국식품위생안전성학회지)
- Volume 6 Issue 3
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- Pages.111-117
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- 1991
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- 1229-1153(pISSN)
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- 2465-9223(eISSN)
Development of Enzyme Linked Immunosorbent Assay for Determination of Zearalenone in Animal Feeds
가축사료 중 Zearalenone 분석을 위한 Enzyme Linked Immunosorbent Assay법의 개발
Abstract
We examined to develop the enzyme linked immunosorbent assay (ELISA) for determination of zearalenone in animal feeds. Zearalenone was first converted to 6'-(carboxymethyl) zearalenone oxime(zearalenone oxime) to get a coupling site and then conjugated to bovine serum albumin(BSA) for use as immunogen and to horseradish peroxidase(HRP) for use as enzyme marker. Antibody against zearalenone was obtained after 11 weeks of immunization of rabbit with zearalenone oxime-BSA. Cross reactivity of the antibody with
가축사료중 zearalenone 분석을 위한 enzyme linked immunosorbent assay(ELISA) 법의 개발을 위해 우선 zearalenone의 항원성을 증폭시키기 위해 zearalenone oxime 유도체를 합성한 다음 bovine serum albumin(BSA)와 conjugate를 만들고, 이를 항원으로 토끼에 면역시켜 11주에 zearalenone에 특이한 항체를 얻어냈다. 생성된 항체를 zearalenone외에
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