• 한국동물학회:뉴스레터
• /
• 제16권1호
• /
• pp.60-65
• /
• 1999

• 한국광학회지
• /
• 제8권4호
• /
• pp.255-259
• /
• 1997

본 연구에서는, Nipkow 원판을 사용하는 공초점 현미겨을 구성하여 시료의 2차원 영상을 얻었으며, 이들을 간단한 알고리즘으로 연결하여 표면의 3차원 영상을 얻었다. 마이크로 필름으로 제작한 Nipkow 원판의 적절한 경로로 통과하는 빛의 투과율은 0.5% 정도였으며, 따라서 반사율이 높은 물체만을 관찰할 수 있었다. 광원으로는 파장 692.7 nm의 반도체 레이저를 사용하였다. 구성된 현미경으로 전산기용 기억소자 칩 표면에 식각된 회로의 깊이와 거칠기를 관찰할 수 있었따. 재생전용 compact disk(CD)도 표면의 보호막을 제거하였을 때 표면에 있는 pit들의 배열 구조가 관찰되었다.

• Restorative Dentistry and Endodontics
• /
• 제26권1호
• /
• pp.1-8
• /
• 2001

Dental Caries which has high prevalence rate, accounts for majority of dental diseases. Many treatment and preventive treatment has been developed, thereby reducing the prevalence rate, but in our country, fluoridization has not spread widely yet, so prevention has not been done satisfactorily. When dental caries progresses, irreversible damage of tooth structure occurs. In initial dental caries, demineralizing tooth structure can be remineralized, so restorative treatment is unnecessary. In this study, 20 teeth restored with composite resin without fluoride release were used and divided into two groups. Incipient dental caries were artificially made and demineralization procedure was done for 1 and 2 weeks, for each group. Changes in mineral contents around the margins were analysed with confocal laser scanning microscope. The results were as follow. 1. Both total fluorescence of the lesion and average fluorescence of the lesion of remineralized samples decreased compared to demineralizing state. (p＜0.01) 2. Confocal laser scanning microscopy can be used in quantitative analysis of mineral change. In result, confocal laser scanning microscopy can be used in quantitative analysis of mineral change and it could be used in many different fields of dentistry in the future.

• 대한소아치과학회지
• /
• 제33권1호
• /
• pp.1-12
• /
• 2006

최근 새로 개발된 DIFOTI(Digital Imaging Fiber-Optic Transillumination) 시스템의 초기 법랑질 우식증 진단 능력을 평가하고 임상 적용의 가능성을 타진해 보고자 본 연구를 시행하였다. 임상 연구는 유치 탈락 시기에 근접한 학령기 아동 23명을 대상으로 DIFOTI 이미지 촬영을 시행하고 자연 탈락된 유치 20개를 수거하여 CLSM 촬영 결과를 바탕으로 민감도(sensitivity)와 특이도(specificity)를 산출하였으며 실험실 연구에서는 수거된 40개의 유치를 대상으로 Carbopol 인공 우식 용액으로 1, 2, 4 그리고 8일간 탈회시킨 후 각각 방사선 사진, DIFOTI 이미지 그리고 CLSM와 비교 분석하여 다음과 같은 결론을 얻었다. 1. 구내에서 촬영한 DIFOTI 이미지의 민감도는 0.61이었고, 특이도는 0.63이었다. 2. 실험실에서 인공 탈회시킨 유치의 협설면 초기 우식증에 대한 DIFOTI 이미지의 민감도는 0.71였고, 특이도는 0.75였다.

• 한국가시화정보학회지
• /
• 제8권1호
• /
• pp.46-52
• /
• 2010

In the present study, we employed the confocal laser scanning microscopy (CLSM) system to visualize the blood flow field with $1{\times}1{\mu}m^2$ spatial resolution. Based on the confocal microscopic image of red blood cells (RBCs), we performed the velocity vector field measurement and evaluated characteristics of cell migration from the cell depleted layer thickness calculation. The rat and mouse's blood were supplied into a micro glass tubes in vitro. The line scanning rate of confocal microscopy was 15 kHz for a $500{\times}500$ pixels image. As a result, the red blood cell itself can be used as a tracer directly without any kind of invasive tracer particle to get the velocity vector field of blood flow by performing particle image velocimetry (PIV) technique.

• 한국생산제조학회지
• /
• 제22권3호
• /
• pp.394-401
• /
• 2013

A new Rockwell hardness (HRC) model using a volumetric parameter by a least square and fractal interpolation method is suggested. The results are also investigated in comparison to real measured hardness data. For this purpose, the measurement of an indented volume is performed using a confocal laser scanning microscope (CLSM), and the captured height encoded image (HEI) is used as an original surface for the calculation of the indented volume. After configuring the surface, the constructed volume is calculated and used as an independent variable for HRC hardness modeling. The hardness model is established using an experimental modeling technique involving a least square algorithm and fractal interpolating model, and this suggested model can be used to reliably predict the Rockwell hardness. These techniques can also be applied to the modeling of the Brinnell and Vickers hardnesses using a volumetric variable.

• 한국지하수토양환경학회:학술대회논문집
• /
• 한국지하수토양환경학회 2004년도 임시총회 및 추계학술발표회
• /
• pp.159-163
• /
• 2004

This study was conducted to calculate the permeability coefficient in a single fracture while taking the true fracture geometry into consideration. The fracture geometry was measured using the confocal laser scanning microscope (CLSM). The CLSM geometry data were used to reconstruct a fracture model for numerical analysis using a homogenization analysis (HA) method. The HA is a new type of perturbation theory developed to characterize the behavior of a micro-inhomogeneous material that involves periodic microstructures. The HA permeability was calculated based on the local geometry and local material properties (water viscosity in this case). The results show that the permeability coefficients do not follow the theoretical relationship of the cubic law.

• 한국멀티미디어학회논문지
• /
• 제24권11호
• /
• pp.1472-1480
• /
• 2021

Image stacking technique is one of the key techniques for complex surface reconstruction. The process includes sample collection, image processing, algorithm editing, surface reconstruction, and finally reaching reliable conclusions. Since this experiment is based on laser scanning confocal microscope to collect the original contour information of the sample, it is necessary to briefly introduce the relevant principle and operation method of laser scanning confocal microscope. After that, the original image is collected and processed, and the data is expanded by interpolation method. Meanwhile, several methods of surface reconstruction are listed. After comparing the advantages and disadvantages of each method, one-dimensional interpolation and volume rendering are finally used to reconstruct the 3D model. The experimental results show that the final 3d surface modeling is more consistent with the appearance information of the original samples. At the same time, the algorithm is simple and easy to understand, strong operability, and can meet the requirements of surface reconstruction of different types of samples.

• 한국광학회:학술대회논문집
• /
• 한국광학회 2005년도 제16회 정기총회 및 동계학술발표회
• /
• pp.242-243
• /
• 2005

• Restorative Dentistry and Endodontics
• /
• 제28권6호
• /
• pp.491-497
• /
• 2003

The purpose of this study was to evaluate the correlation between hybrid layer thickness and bond strength using confocal laser scanning microscope and microtensile bond strength test of two adhesive systems. The dentin surface of human molars. sectioned to remove the enamel from the occlusal surface. Either Scotchbond Multi-Purpose(3M Dental Product, St. Paul, MN, U.S.A) or Clearfil SE Bond (Kuraray, Osaka, Japan) was bonded to the surface. and covered with resin-composite. The resin-bonded teeth were serially sliced perpendicular to the adhesive interface to measure the hybrid layer thickness by confocal laser scanning microscope. The specimen were trimmed to give a bonded cross-sectional surface area of $1\textrm{mm}^2$, then the micro-tensile bone test was performed at a cross head speed of 1.0 mm/min. All fractured surfaces were also observed by stereomicroscope. There was no significant differences in bond strengths the materials(p>0.05). However. the hybrid layers of three-step dentin adhesive system, SM, had significantly thicker than self-etching adhesive system. CS(p<0.05). Pearson's correlation coefficient showed no correlation between hybrid layer thickness and bond strengths(p>0.05). Bond strengths of dentin adhesive systems were not dependent on the thickness of hybrid layer.