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http://dx.doi.org/10.5407/JKSV.2010.8.1.046

Flow Visualization of Blood Cell and Detection of Cell Depleted Layer Using a Confocal Laser Scanning Microscope  

Lim, Soo-Hee (경북대학교 대학원 기계공학과)
Kim, Wi-Han (경북대학교 대학원 기계공학과)
Lee, Ho (경북대학교 기계공학부)
Lee, Choon-Young (경북대학교 기계공학부)
Park, Cheol-Woo (경북대학교 기계공학부)
Publication Information
Journal of the Korean Society of Visualization / v.8, no.1, 2010 , pp. 46-52 More about this Journal
Abstract
In the present study, we employed the confocal laser scanning microscopy (CLSM) system to visualize the blood flow field with $1{\times}1{\mu}m^2$ spatial resolution. Based on the confocal microscopic image of red blood cells (RBCs), we performed the velocity vector field measurement and evaluated characteristics of cell migration from the cell depleted layer thickness calculation. The rat and mouse's blood were supplied into a micro glass tubes in vitro. The line scanning rate of confocal microscopy was 15 kHz for a $500{\times}500$ pixels image. As a result, the red blood cell itself can be used as a tracer directly without any kind of invasive tracer particle to get the velocity vector field of blood flow by performing particle image velocimetry (PIV) technique.
Keywords
Confocal Laser Scanning Microscopy; Red Blood Cell; Blood Flow; Cell Depleted Layer; PIV;
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