Journal of the Korean Society of Visualization (한국가시화정보학회지)

The Korean Society of Visualization (한국가시화정보학회)
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Flow Visualization of Blood Cell and Detection of Cell Depleted Layer Using a Confocal Laser Scanning Microscope

공초점 레이저 주사 현미경을 이용한 혈구 유동가시화 및 세포공핍층 측정에 관한 연구

  • 임수희 (경북대학교 대학원 기계공학과) ;
  • 김위한 (경북대학교 대학원 기계공학과) ;
  • 이호 (경북대학교 기계공학부) ;
  • 이춘영 (경북대학교 기계공학부) ;
  • 박철우 (경북대학교 기계공학부)
  • Received : 2010.03.04
  • Accepted : 2010.03.26
  • Published : 2010.03.31
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Abstract

In the present study, we employed the confocal laser scanning microscopy (CLSM) system to visualize the blood flow field with $1{\times}1{\mu}m^2$ spatial resolution. Based on the confocal microscopic image of red blood cells (RBCs), we performed the velocity vector field measurement and evaluated characteristics of cell migration from the cell depleted layer thickness calculation. The rat and mouse's blood were supplied into a micro glass tubes in vitro. The line scanning rate of confocal microscopy was 15 kHz for a $500{\times}500$ pixels image. As a result, the red blood cell itself can be used as a tracer directly without any kind of invasive tracer particle to get the velocity vector field of blood flow by performing particle image velocimetry (PIV) technique.

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References

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