• Journal of Korean Medicine for Obesity Research
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• v.15 no.1
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• pp.38-44
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• 2015

Objective: Skeletal muscle is a crucial tissue from the perspectives of mitochondrial dysfunction and insulin resistance, it is formed by myogenesis which is dynamic multistep process to be myotubes. The authors could found that root of Atractylodes macrocephala Koidzumi (Atractylodis Rhizoma Alba, ARA) enhanced glucose and lipid metabolism in C2C12 myotubes via mitochondrial regulation. However its action in myogenesis process is not known. The aim of this work was the study of ARA on proliferation, differentiation and hypertrophy in C2C12 cells. Methods: To study proliferation phase, cells were incubated in growth medium with or without ARA (0.2 or 1.0 mg/ml) for 24 hours. To examine differentiation, at 70% confluence, cells were transferred in differentiation medium both with/without ARA (0.2 or 1.0 mg/ml) for 96 hours. And after 72 hours of differentiation, cells were treated with or without ARA (0.2 or 1.0 mg/ml) for 24 hours, the genesis of hypertrophy in myotubes were analyzed. Results: In proliferation phase, ARA could make difference in morphologic examination. In differentiation phase, it also made morphologic difference furthermore ARA (1.0 mg/ml) increased mRNA expressions of Myogenic regulatory factors and muscle-specific proteins synthesis. In late differentiation, ARA induced hypertrophic morphological changes in neo-formed myotubes. Conclusions: ARA might control cell cycle promoting myogenesis and hypertrophy in C2C12 cells.

• Korean Journal of Exercise Nutrition
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• v.23 no.1
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• pp.7-12
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• 2019

[Purpose] The purpose of the study was to determine the combined effects of resveratrol supplementation with high-flow LSS on mitochondrial biogenesis in human vascular endothelial cells. [Methods] Cultured human umbilical vein endothelial cells were treated with 20 μM of RSV. For the shear experiments, cells grown to a >90% confluence were exposed to physiological levels of LSS (5 to 20 dyne/cm2) for 12 to 36 hours using a cone and plate shear apparatus. Gene expressions were analyzed by western blotting. [Results] Depletion of mitochondrial integrity was directly associated with increase in endothelial activation/dysfunction. The expressions of mitochondrial biogenesis regulator genes, such as SIRT1, PGC-1α, and TFAM, and the mitochondrial contents were significantly increased after treatment with both resveratrol and high-flow LSS for 12 hours. However, supplementation of resveratrol to high-flow LSS for a prolonged duration had no synergistic effect on the levels of mitochondrial biogenesis regulator gene expressions and mitochondrial content compared to the LSS treatment alone. [Conclusion] The present study demonstrated that the supplementation of resveratrol to high-flow LSS has no synergistic effects on enhancing mitochondrial integrity in human vascular endothelial cells.

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.624-628
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• 2008

Two-dimensional flow analysis is a way to provide good estimates for complex flow features in flow around islands and obstructions, flow at confluence and flow in braided channel. One of difficult problems to develop a two-dimensional hydraulic model is to analyze dry and wet area in river channel. Dry/wet problem can be encountered in river and coastal engineering problems, such as flood propagation, dam break analysis, tidal processes and so on. The objective of this study is to develop an accurate and robust two-dimensional finite element method with dry/wet technique in complex natural rivers. The dry/wet technique with Deforming Grid Method was developed in this study. The Deforming Grid Method was used to construct new mesh by eliminating of dry nodes and elements. The eliminated nodes and elements were decided by considering of the rising/descending velocity of water surface elevation. Several numerical simulations were carried out to examine the performance of the Deforming Grid Method for the purpose of validation and verification of the model in rectangular and trapezoidal channel with partly dry side. The application results of the model were displayed reasonable flow distribution.

• Journal of Korean Society of Rural Planning
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• v.24 no.2
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• pp.21-29
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• 2018

Riparian management has become important as stream water quality as well as riparian ecosystem gain more public attentions. The objective of this study was to determine riparian widths based on the functions of nutrient removal and wildlife habitat protection and to apply for the Jinwee stream area as a preliminary case. Nitrogen and phosphorus filtration efficiencies were considered in water quality aspect, while the habitat radii of amphibian and reptiles were used for wildlife conservation purpose. In addition, observation of endangered species and human impact on wildlife disturbance in riparian area were also taken into account in determining riparian widths. The stream confluence zone was emphasized by doubling the riparian widths as the focal point for wildlife habitat conservation. As the results, three different levels of riparian widths were proposed depending on the major riparian functions and applied to the Jinwee stream section as the case study. The proposed method can be used to determine riparian width in other stream areas based on different functional focus, ie, water quality or riparian conservation purposes.

• Journal of Korean Society for Geospatial Information Science
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• v.5 no.2 s.10
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• pp.145-152
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• 1997

A coupling of two-dimensional model and GIS technique is applied to simulate sediment transport analysis. Accurate transformations are performed for the data which have various reduced scales. Basic maps are discretized the mesh of true scale for the finite element model by using the digital map and the Traverse Mercator coordinate. Under $200m^3/s$ flow rate condition, velocity vectors, depth contours, velocity contours and deposition contours are Presented for the 5km reach of midstream of Keum River from confluence of Ji-chun to Baekche Bridge. The simulation results of the study agree well with those of one-dimensional varied flow analysis and observed data. The flow and deposition pattern revealed a meandering characteristics of the river qualitatively.

• Proceedings of the Korea Water Resources Association Conference
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• 2004.05b
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• pp.908-911
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• 2004

The purpose of this study is to investigate the applicability of the two dimensional model in natural rivers. In this study, two dimensional unite element model, SMS, is used to simulate a complex flow along with the sediment movements in the natural river. The RMA-2 model embeded in SMS is used to simulate flow phenomena and SED-2D model is employed to simulate sediment transport. The model is applied to the confluence zone of the Gam River and mouth of Nakdong River. For model calibration, the result of the unsteady flow analysis is compared with the Typhoon 'Rusa' data. In addition, the runoff analysis was conducted for the determination of the project flood and the flood forecasting. The simulation results presented the characteristics of two dimensional flow with velocity vector and flow depth. The sediment transport characteristics are shown in terms of sediment concentration as well as bed elevation change. Accordingly, the SMS model in this study turned out to be very effective tool for the simulation of the hydrodynamic characteristics under the various flow conditions and corresponding sediment transports in natural rivers.

• Computers and Concrete
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• v.31 no.2
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• pp.163-171
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• 2023

Face gear transmission is widely used in aerospace shunt-confluence transmission system. Tooth wear is one of the main factors affecting its bearing transmission performance. Furthermore, the installation errors of face gear are inevitable. In order to study the wear mechanism of face gear tooth surface with installation errors, based on tooth contact analysis numerical method and Archard wear theory, the UMESHMOTION subroutine in ABAQUS is developed.Combining with Arbitrary Lagrangian-Eulerian adaptive mesh technology, the finite element mesh wear model of abraded face gear pair is established.The preprocessing conditions are set to generate the inp files.Then,the inp files for each corner are imported and batch processed in ABAQUS.The loading tooth contact problem at each rotation angle is solved and the load distribution coefficient among gear tooth, tooth root bending stress, tooth surface contact stress and loaded transmission error are obtained. Results show that the tooth root wear is the most serious and the wear at the pitch cone is close to 0.The wear law of tooth surface along tooth width direction is convex parabola and the wear law along tooth height direction is concave parabola.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.6
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• pp.777-784
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• 2001

Evaluation of the granulosa cell (GC) monolayers developed from small (<5 mm) and large (>5 mm) follicles on the meiotic competence of caprine oocytes during in vitro maturation was done in this study in comparison to the granulosa cell coculture. Ovaries were collected from the local abattoir and follicular contents were aspirated for the monolayer culture. For IVM the oocytes were collected by puncturing the nonatretic follicles (>4 mm). Results revealed that at the same seeding rate, small follicular granulosa cell monolayer achieved confluence 24-48 h earlier than large follicular granulosa cell monolayer. GC monolayers significantly p (<0.05) improved the rate of meiotic resumption and nuclear maturation (84.76% vs 74.74%) after 27 h of culture in comparison to GC coculture. Statistically there was no significant difference in the maturation rate between the caprine oocytes matured over small or large follicular GC monolayers. It is concluded from the present study that GC monolayers support better nuclear and cytoplasmic maturation of growing caprine oocytes which is evident by better maturation rate over GC monolayer as compared to the oocytes matured with GC coculture. Granulosa cells from small and large follicles can be used for IVM with more or less in the same efficiency after conditioning them with maturation media in 18-24 h before the onset of culture.

• Journal of Industrial Technology
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• v.29 no.A
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• pp.111-122
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• 2009

In this study, flood stage was computed by HEC-RAS, 1-D numerical analysis model and FESWMS-2DH, 2-D numerical model. Flood stages computed by two different models were compared for straight line, dot axle watercourse, dead-zone watercourse, section sudden-changing watercourse, and curved watercourse. From the results, flow velocity and water level were similar in straight watercourse and dot-reduction watercourse. However, there was difference of flow velocity and water level in dead-zone watercourse, sudden expansion, dot-reduction, and curve-watercourse. This result might be influenced by rapid change of watercourse due to dead-zone, the angle of inflow and outflow, and the curvature. Especially in this study, numerical model was applied to Wol-Song-Cheon at Chuncheon in order to analyze the effect of flood stage by two different models. By flowing properties around the bridge and confluence of rivers, it was found that flow velocity and water level was changed. Therefore, it was concluded that a lot of uncertainties are contained in the present bank.

• Journal of Korean Society for Geospatial Information Science
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• v.20 no.1
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• pp.109-116
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• 2012

The effect of the grid resolution on the hydrodynamic simulation has been investigated by using CCHE2D and EFDC. Since a high resolution of the grid results in the increase of computation time, an appropriate grid resolution should be selected by considering the efficiency of simulation according to the objectives of projects. In order to understand the effect of grid resolution and determine the optimal grid resolution, several cases with different lateral grid resolutions have been simulated for the reach of Nakdong river at the confluence of Kumho river for the floods in 2006. Orthogonal curvilinear grids for the domain have been constructed from the survey products at the sections with the longitudinal interval of 20 m. Area-elevation curve and the comparison of simulated results with measured stage at the specific station have been used to check the effect of grid resolution. From the results, the existence of optimal grid resolution has been observed, which ensure both efficiency of computation and certainty of results.