• 한국식품과학회지
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• 제51권2호
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• pp.188-191
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• 2019

In this study, the poor electro-transformant yield of large DNA in bifidobacteria was improved by increasing the DNA concentration, which was amplified by enhancing electroporation conditions: treating the cell wall weakening agent and cell membrane permeabilizing molecule as well as changing the electrical parameter. In the enhanced conditions, the electroporation frequency increased from 15 to 71 times compared to the initial conditions at the same DNA concentration. As the DNA concentration increased, the difference in the electroporation frequency between the two conditions became greater, and the curve of the enhanced conditions seemed to be saturated with a DNA concentration over $4{\mu}g$. The present study provided a clue to the recovery of the electroporation frequency with large DNA and formulated the relationship between the DNA concentration, the DNA size and the electroporation frequency in bifidobacteria. Therefore, this study will contribute to the expansion of molecular genetic studies of bifidobacteria.

• Journal of Microbiology and Biotechnology
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• 제17권1호
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• pp.146-153
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• 2007

A mathematical competition model between normal flora and an invading pathogen was devised to allow analysis of bacterial infections in a host. The normal flora includes the various microorganisms that live on or within the host and act as a primary human immune system. Despite the important role of the normal flora, no mathematical study has been undertaken on models of the interaction between it and invading pathogens against a background of antibiotic treatment. To quantify key elements of bacterial behavior in a host, pairs of nonlinear differential equations were used to describe three categories of human health conditions, namely, healthy, latent infection, and active infection. In addition, a cutoff value was proposed to represent the minimum population level required for survival. The recovery of normal flora after antibiotic treatment was also included in the simulation because of its relation to human health recovery. The significance of each simulation parameter for the bacterial growth model was investigated. The devised simulation showed that bacterial proliferation rate, carrying capacity, initial population levels, and competition intensity have a significant effect on bacterial behavior. Consequently, a model was established to describe competition between normal flora and an infiltrating pathogen. Unlike other population models, the recovery process described by the devised model can describe the human health recovery mechanism.

• 한국지구물리탐사학회:학술대회논문집
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• 한국지구물리탐사학회 2003년도 Proceedings of the international symposium on the fusion technology
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• pp.581-589
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• 2003

The variable flotation response ores from different deposits results basically from mineralogical association and their differences. Development of new techniques for analyzing the metallurgical performance of flotation and other concentration processes is demanded even in the treatment of rather simple ores such as porphyry ores. Diagnostic metallurgical analysis can be used to quantify the most possible recovery processes. Several porphyry copper/gold ores around the world were used to examine the responses in flotation, gravity separation and cyanidation in order to define the linkage between the recovery processes for both copper and old values. Laboratory batch flotation, gravity separation and cyanidation tests were carried out on these samples. All results were used to correlate the relative recovery of copper and gold, and to predict the highest possible metal recovery in the system. The metallurgical predictions were made according to the flotation conditions used and gravity separation. The results of various concentration processes on each porphyry ore samples are presented and discussed. All seven samples have shown significantly different gold/copper metallurgy. The grade/recovery relationships of gold and copper in the laboratory batch tests for the best results and the plants are given in the Figures below. The results of laboratory tests show that the copper recoveries converged to about $90\%$, but the gold recoveries were spread over $55-80\%$, except the K S ore. Series of standard cyanidation tests on the flotation concentrate samples and gravity separation using Knelson Separator on heads ores were carried out to cross-link the metallurgy and mineralogy of gold in the porphyry ores.

• Journal of Microbiology and Biotechnology
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• 제28권5호
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• pp.739-747
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• 2018

The low solubility and high-cost recovery of Propionibacterium acnes polyunsaturated fatty acid isomerase (PAI) are key problems in the bioproduction of high value-added conjugated linoleic acid (CLA). To improve the solubility of recombinant PAI, six chaperone proteins were coexpressed with PAI. Introduction of GroELS proteins dramatically improved the PAI solubility from 29% to 97%, with increased activity by 57.8%. Combined expression of DnaKJ-GrpE and GroELS proteins increased the activity by 11.9%. In contrast, coexpression of DnaKJ-GrpE proteins significantly reduced the activity by 57.4%. Plasmids pTf16 harboring the tig gene and pG-Tf2 containing the tig and groEL-groES genes had no visible impact on PAI expression. The lytic protein E was then introduced into the recombinant Escherichia coli to develop a cell autolysis system. A 35% activity of total intracellular PAI was released from the cytoplasm by suspending the lysed cells in distilled water. The PAI recovery was further improved to 81% by optimizing the release conditions. The lipase from Rhizopus oryzae was also expressed in E. coli, with an extracellular activity of 110.9 U/ml. By using the free PAI and lipase as catalysts, a joint system was established for producing CLA from sunflower oil. Under the optimized conditions, the maximum titer of t-10,c-12-CLA reached 9.4 g/l. This work provides an effective and low-cost strategy to improve the solubility and recovery of the recombinant intracellular PAI for further large-scale production of CLA.

• 대한조선학회논문집
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• 제53권3호
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• pp.195-200
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• 2016

In this study, compression tests for the polyisocyanurate foam which is recognized as the insulation material for the storage tank of the liquefied natural gas (LNG) were carried out for investigation of the material characteristic of mechanical behavior. Deformation recovery ratio according to the various initial deformation levels were estimated and the mechanical behavior of foams that are experienced compressive deformation was also obtained experimentally. The test results were analyzed based on the conditions of initially applied strain level and engineering strain rate.

• 한국습지학회지
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• 제12권2호
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• pp.87-93
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• 2010

하천의 다양성을 반영할 수 있는 하천복원 계획수립 및 선정방법이 필요하다. 한편, 하천복원의 궁극적인 목적은 하천 본래의 자연성 회복에 있으며, 최근에는 친수적 증진을 포함하는 추세이다. 본 연구에서는 자연형 하천복원 대안선정에 하천자연도을 적용하였다. 또한 하천자연도는 국내실정에 맞도록 다기준 의사결정을 활용하여 개발된 하천자연도평가법을 사용하여 산정하였다. 대상하천의 하천자연도 평가항목은 하천의 특성을 고려하여 선택한 후, 복수의 대안을 구성하고 대안별 하천자연도를 산정하여 비교하면, 각 대안별 자연성 회복효과 및 성과를 측정하는 것이 용이하였다.

• 한국축산식품학회지
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• 제41권2호
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• pp.335-342
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• 2021

Vitamin B12 deficiency may lead to serious health issues in both infants and adults. A simple analytical method involving sample pretreatment with enzyme, followed by cyanide addition under acidic conditions; separation on an immunoaffinity column; and high-performance liquid chromatography (HPLC) was developed for the rapid detection and quantitation of vitamin B12 in powdered milk. Detection limit and powdered milk recovery were determined by quantitative analysis. The limits of detection and quantitation were 2.71 and 8.21 ㎍/L, respectively. Relative standard deviations of the intra-day and inter-day precisions varied in the ranges of 0.98%-5.31% and 2.16%-3.90%, respectively. Recovery of the analysis varied in the range of 83.41%-106.57%, suggesting that the values were acceptable. Additionally, vitamin B12 content and recovery in SRM 1849a were 54.10 ㎍/kg and 112.24%, respectively. Our results suggested that the analytical method, including the sample pretreatment step, was valid. This analytical method can be implemented in many laboratory-scale experiments that seek to save time and labor. Therefore, this study shows that immunoaffinity-HPLC/ultraviolet is an acceptable technique for constructing a reliable database on vitamin B12 in powdered milk containing starch as well as protein and/or fat in high amounts.

• ETRI Journal
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• 제38권2호
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• pp.244-251
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• 2016

In this paper, we investigate the electrical characteristics of two trench-gate-type super-barrier rectifiers (TSBRs) under different p-body implantation conditions (low and high). Also, design considerations for the TSBRs are discussed in this paper. The TSBRs' electrical properties depend strongly on their respective p-body implantation conditions. In the case of the TSBR with a low p-body implantation condition, it exhibits MOSFET-like properties, such as a low forward voltage ($V_F$) drop, high reverse leakage current, and a low peak reverse recovery current owing to a majority carrier operation. However, in the case of the TSBR with a high p-body implantation condition, it exhibits pn junction diode.like properties, such as a high $V_F$, low reverse leakage current, and high peak reverse recovery current owing to a minority carrier operation. As a result, the TSBR with a low p-body implantation condition is capable of operating as a MOSFET, and the TSBR with a high p-body implantation condition is capable of operating as either a pn junction diode or a MOSFET, but not both at the same time.

• Journal of the Korean Wood Science and Technology
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• 제18권1호
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• pp.15-23
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• 1990

Roughage feeds were produced from Hyun-aspen (Populus alba $\times$ P. glandulosa) by autohydrolysis. The objectives of this work were to find proper conditions for the treatment of Hyun-aspen by analyzing the compositional change and digestibility and to determine the content of sugar and phenol contained in liquor extracted by digestion. The results of this work were as follows: 1. The proper condition for autohydrolysis of Hyun-aspen chips were $160^{\circ}C$ and 30 minutes in an autoclave. The yield of potential feed from original material and digestibility were 91.3% and 38.9% respectively 2. According to cooking conditions the sugar concentration of extracted solution and the recovery rate of sugar were 0.1~2.5%, 0.3~14.6% respectively. The phenol concentration of extracted solution and the recovery rate of phenol were 0.1~0.3, 0.5~1.8% respectively.

• Geomechanics and Engineering
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• 제11권6호
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• pp.879-896
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• 2016

A new earth pressure equation considering the arching effect in $c-{\phi}$ soils was proposed for the accurate calculation of earth pressure on circular vertical shafts. The arching effect and the subsequent load recovery phenomenon occurring due to multi-step excavation were quantitatively investigated through laboratory tests. The new earth pressure equation was verified by comparing the test results with the earth pressures predicted by new equation in various soil conditions. Resulting from testing by using multi-step excavation, the arching effect and load recovery were clearly observed. The test results in $c-{\phi}$ soil showed that even a small amount of cohesion can cause the earth pressure to decrease significantly. Therefore, predicting earth pressure without considering such cohesion can lead to overestimation of earth pressure. The test results in various ground conditions demonstrated that the newly proposed equation, which enables consideration of cohesion as appropriate, is the most reliable equation for predicting earth pressure in both ${\phi}$ soil and $c-{\phi}$ soil. The comparison of the theoretical equations with the field data measured on a real construction site also highlighted the best-fitness of the theoretical equation in predicting earth pressure.