• Journal of Biomedical Engineering Research
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• v.38 no.6
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• pp.302-307
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• 2017

This paper presents a paper-based concentration gradient chip to analyze colorimetric glucose assay. The paper-based concentration gradient chip was fabricated through a wax patterning technique that can design the fluidic channel by selectively printing hydrophobic and hydrophilic areas. Afterwards, glucose and dilution solutions were loaded into the inlet of a concentration gradient chip and each solution was then mixed sequentially at mixing channel. Finally, concentration gradient was formed at each outlet of the chip. To measure the glucose concentration of the solution in outlets, we conducted colorimetric glucose assay with fixed concentration of glucose solution (0, 5, 10, 15 and 20 mM) and obtained normalized intensity. Subsequently, glucose concentrations of the outlets were calculated by substituting the normalized intensity to linear regression function based on the normalized intensity of fixed glucose concentration. Finally, the concentration gradient of glucose was formed on the chip with the result of colorimetric assay. The concentration gradient paper chip has the potential to accurately analyze unknown glucose concentration.

• 한국연소학회:학술대회논문집
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• 2002.06a
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• pp.99-105
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• 2002

The concentration gradient effects on triple flame have been studied experimentally using a slot burner in order to stabilize stationary triple flame in coflowing stream. By means of contraction we generate the coflowing stream with uniform velocity and linear concentration gradient at the outlet of the slot. In this paper we investigated the response of the triple flame. to the concentration gradient, like the stability, the liftoff height, and the structure of triple flame. Flow velocity is measured with Laser Doppler Velocimetry. As the concentration gradient increases. the flame propagation velocity in immediately upstream triple point increases until the liftoff height of triple flame becomes minimum, and then decreases.

• Asian Journal of Turfgrass Science
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• v.10 no.1
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• pp.61-69
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• 1996

The distribution of salt plant communities is conditioned by the gradient of salt concentration in the tidal land. The quantitative analyses of the salt vegetation were made to investigate the continuum in different stands of the tidal land in Namdong, Incheon City. The order in which the leading dominances are arranged is decided by the gradient of salt concentration. Wading dominant species, from the high level to the low level of the salt gradient, were Salicornia herbatacea, Sueda maritma, Limonium tetragonum, Aster tripolium, Atriplex tatarica, Catystegia soldonella, and Lathylus japonica These species form a continuum on the tidal land depending upon salt concentration. Key words: Continuum distribution, Salt gradient, Wading dominance.

• 한국연소학회:학술대회논문집
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• 2004.06a
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• pp.7-14
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• 2004

The lift-off characteristics of the triple flame have been studied experimentally with various mean velocities and concentration gradients using a multi-slot burner, which can control the concentration gradient and the mean velocity independently, Lift-off height, axial maximum velocity, flame temperature, and some other characteristics were examined for methane and propane flame, It was found that minimum values of the lift-off heights exist at a certain concentration gradient for constant mean velocity, and this result implies that the propagation velocity has a maximum value at this condition, OH radical distribution was measured with LIF method and velocity variation along streamline was measured with PlV system. In addition maximum temperature along streamline was measured with CARS system. The intensity of the diffusion flame affects on the propagation velocity of triple flame in the region of very weak concentration gradient.

• 한국연소학회:학술대회논문집
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• 2003.05a
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• pp.85-91
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• 2003

The lift-off characteristics of the triple flame within a diverging duct have been studied experimentally using a multi-slot burner, which can control the concentration gradient and the mean velocity independently. In this experiment the triple flame was stabilized successfully in lift-off condition and flame stabilization with a duct or without a duct, lift-off heights, and some other characteristics were examined for propane flame. It was examined that the effects with various concentration gradient and mean velocity on the triple flame. It was found that minimum value of the lift-off heights exist at a certain concentration gradient for constant mean velocity and flame with a duct is more stable than that without. Moreover the propagation velocity of the flame becomes maximum at a certain concentration gradient regardless of mean velocity.

• Macromolecular Research
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• v.11 no.6
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• pp.467-470
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• 2003

The dielectrophoretic technique was used to prepare density gradient polymers, polystyrene doped with a dipolar modifier, diphenyl sulfide. We have measured concentration gradients of the dopant by UV/Nis spectroscopy as a function of time in a nonuniform electric field. Measured concentration data at different positions of the sample confirmed that a concentration gradient arose after a nonuniform electric field was applied to the system, these data were used to compare the concentration profile with that predicted by the dielectrophoresis equation.

• Biomolecules & Therapeutics
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• v.26 no.4
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• pp.380-388
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• 2018

Neural stem cells (NSCs) have the ability to self-renew and differentiate into multiple nervous system cell types. During embryonic development, the concentrations of soluble biological molecules have a critical role in controlling cell proliferation, migration, differentiation and apoptosis. In an effort to find optimal culture conditions for the generation of desired cell types in vitro, we used a microfluidic chip-generated growth factor gradient system. In the current study, NSCs in the microfluidic device remained healthy during the entire period of cell culture, and proliferated and differentiated in response to the concentration gradient of growth factors (epithermal growth factor and basic fibroblast growth factor). We also showed that overexpression of ASCL1 in NSCs increased neuronal differentiation depending on the concentration gradient of growth factors generated in the microfluidic gradient chip. The microfluidic system allowed us to study concentration-dependent effects of growth factors within a single device, while a traditional system requires multiple independent cultures using fixed growth factor concentrations. Our study suggests that the microfluidic gradient-generating chip is a powerful tool for determining the optimal culture conditions.

• Journal of Industrial and Engineering Chemistry
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• v.68
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• pp.355-363
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• 2018

There are serious issues with the application of surfactant flooding as a third recovery method, such as surfactant slug losses. In this study,the impact of the salinity gradient on the remobilization of oiltrapped in Berea sandstone was investigated by emphasizing the surfactant adsorption gradient and phase behavior to determine the optimal salinity of the chosen surfactant concentration for investigating the salinity gradient. Three salinity-gradient schemes were applied to six cores saturated with light and heavy oils. The positive salinity gradient provided the best recovery results with an in situ microemulsion formation that could be observed in the fluid collector.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2001.10a
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• pp.160-163
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• 2001

In order to develop a nodule conveying system through a flexible pipe out of the deep-seabed manganese nodule miner, an experimental study of the solid-water slurry flow in vertical pipe is performed as the first stage of total experiments. Hydraulic characteristics of the pipe slurry flow such as slip velocity, transport concentration and pressure gradient are investigated for the size of particle, load ratio, and flow rate of water. The higher the load ratio is, the larger the transport concentration and pressure gradient become. The bigger the size of particles is, the larger the pressure gradient becomes. The effectiveness of the flow rate to hydraulic performance is also investigated. This results are to be used for designing crusher and pump, and operating the conveying device.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.8
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• pp.1061-1070
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• 2003

A triple flame in a mixing layer was studied experimentally with concentration gradient and mean velocity by using a multi-slot burner, which can stabilize the lift-off flame. Flame stabilization condition, lift-off heights, and some other characteristics were examined for methane and propane flame within a range of very low concentration gradient. Pitot-tube and LDV(Laser Doppler Velocimetry) were used for velocity. Mass spectroscopy and Rayleigh scattering signal were used for concentration gradients. Thermo-couples and SiC TFP(Thin Filament Pyrometer) were used for temperature. It was found that minimum values of the lift-off heights exist at a certain concentration gradient for constant mean velocity and this means that the propagation velocity has a maximum value. The scales of flame to the burner nozzle and intensity variation of the diffusion flame were suspected as the cause.