Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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A Colorimetric Glucose Assay via Concentration Gradient Paper Chip

종이기반 농도 구배 형성 칩을 통한 포도당 발색 반응 검사

  • Kim, Taehoon H. (Dept. of Biomedical Engineering, Konyang University) ;
  • Shin, Hyun Young (Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology(DGIST)) ;
  • Lee, Yun-Il (Well Aging Research Center, Daegu Gyeongbuk Institute of Science and Technology(DGIST)) ;
  • Tae, Ki-Sik (Dept. of Biomedical Engineering, Konyang University) ;
  • Kim, Minseok S. (Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology(DGIST))
  • 김태훈 (건양대학교 의공학부) ;
  • 신현영 (대구경북과학기술원 뉴바이올로지학과) ;
  • 이윤일 (대구경북과학기술원 웰에이징연구소) ;
  • 태기식 (건양대학교 의공학부) ;
  • 김민석 (대구경북과학기술원 뉴바이올로지학과)
  • Received : 2017.11.16
  • Accepted : 2017.12.13
  • Published : 2017.12.31
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Abstract

This paper presents a paper-based concentration gradient chip to analyze colorimetric glucose assay. The paper-based concentration gradient chip was fabricated through a wax patterning technique that can design the fluidic channel by selectively printing hydrophobic and hydrophilic areas. Afterwards, glucose and dilution solutions were loaded into the inlet of a concentration gradient chip and each solution was then mixed sequentially at mixing channel. Finally, concentration gradient was formed at each outlet of the chip. To measure the glucose concentration of the solution in outlets, we conducted colorimetric glucose assay with fixed concentration of glucose solution (0, 5, 10, 15 and 20 mM) and obtained normalized intensity. Subsequently, glucose concentrations of the outlets were calculated by substituting the normalized intensity to linear regression function based on the normalized intensity of fixed glucose concentration. Finally, the concentration gradient of glucose was formed on the chip with the result of colorimetric assay. The concentration gradient paper chip has the potential to accurately analyze unknown glucose concentration.

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References

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