• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제53권7호
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• pp.364-369
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• 2004

In this Paper, a flashover prediction method using the leakage current in the contaminated EPDM distribution polymer insulator is proposed. The leakage currents on the insulator were measured simultaneously with the different salt fog application such as 25g, 50g, and 75g per liter of deionized water. Then, the measured leakage currents were enveloped and transformed as the CDFS using the Hilbert transform and the level crossing rate, respectively. The obtained CDFS having different gradients(angles) were used as a important factor for the flashover prediction of the contaminated polymer insulator. Thus, the average angle change with an identical salt fog concentration was within a range of 20 degrees, and the average angle change among the different salt fog concentrations was 5 degrees. However, it is hard to be distinguished each other because the gradient differences among the CDFS were very small. So, the new weighting value was defined and used to solve this problem. Through simulation, it Is verified that the proposed method has the capability of the flashover prediction.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2001년도 학술 발표회 진행표 및 논문초록
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• pp.45-45
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• 2001

The Na$^{＋}$-K$^{＋}$ pump, a plasma membrane Na$^{＋}$-K$^{＋}$ ATPase is plays a key role in the regulation and maintenance of Na$^{＋}$ and $K^{＋}$ ion concentration gradients across cell membranes. This enzyme pumps three Na$^{＋}$ out of and two $K^{＋}$ into the cell against their electrochemical gradient by utilizing the energy derived from ATP. Therefore, the Na$^{+}$-K$^{+}$ pump generates a net outward electrical current.(omitted)ted)

• Journal of Plant Biology
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• 제36권2호
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• pp.171-176
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• 1993

Protein kinase C, a protein related in PI cascade, was partially purified from the cytosol protein of etiolated plants of Glycine max by DEAE-52 cellulose chromatography and phenylsepharose chromatography. When the DEAE column was eluted with 0-0.8 M linear gradient KCl, tow fractions were found that increased the phosphorylation of histon H1 about five and nine-fold in the presence of 5 $\mu\textrm{g}$/mL phosphatidylserine and 0.5 $\mu\textrm{g}$/mL diolein, respectively. These fractions were used as DEAE pool. The reaction eluted with relatively high concentration of KCl was loaded on phyenylsepharose column with 5 mM CaCl2 and eluted with 1 mM EGTA. A fraction contained the protein kinase C, which increased the phosphorylation of the histon H1 was fractionated. To determine the molecular weight of PKC, the fraction eluted from phenylsepharose column was analyzed by 5~15% polyacrylamide gel electrophoresis after concentrated with the Amicon membrane (YM10). That revealed two bands corresponding to 60 and 65 kGy by silver staining of the gel, respectively.

• International Journal of Industrial Entomology and Biomaterials
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• 제27권2호
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• pp.322-325
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• 2013

In the present study, we report that the selection of operation mode is important to take the full advantage of nanofibrous membrane in enzyme immobilization. Silk fibroin nanofibrous membrane has been prepared by electrospinning, and a-chymotrypsin was immobilized as a model enzyme. When the immobilized enzyme was operated in the membrane reactor mode, the Michaelis constant, Km, was lower and the Vmax was higher compared to the batch reactor mode. No concentration gradient was observed in the membrane reactor mode and the immobilized enzyme was stable even after 7 times of re-use. Our results suggests that the enzyme immobilized nanofibrous membrane should be operated in the membrane reactor mode rather than in the bath reactor mode.

• 대한기계학회논문집B
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• 제22권7호
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• pp.1022-1029
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• 1998

The relation of the inlet fuel distribution, velocity, and overall equivalence ratio to the stability of a lean burning no-swirl dump combustor was examined. Premixed or partially premixed natural gas was introduced into the air stream, which flowed to the dump region through an annular inlet pipe. Inlet air was preheated upto 400 deg.C. Combustion instability was observed to occur at higher value of equivalence ratio (> 0.6) as the degree of unpremixedness was increased. Instabilities exhibited a dominant frequency of ~ 500 Hz, which corresponded to a half wave mode of combustor. CH chemiluminescence and pressure fluctuations were in-phase when combustion instabilities occurred. Acetone LIF images revealed that there was a strong fuel concentration gradient across the inlet annulus. Phase resolved OH LIF images showed that inlet fuel distribution was affected by the combustion instabilities.

• 한국자동차공학회논문집
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• 제3권5호
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• pp.111-121
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• 1995

The initiation and propagation of detonation waves in the air/fuel spray mixture has been numerically analyzed. An improved pressure-based method has been applied to predict the transient heterogeneous reacting flows at all speeds. Numerical results indicate that variations in the temperature gradient, the droplet size, and the fuel vapor concentration have the significant effects on the development of detonation wave in the multi-phase reactive media. The interaction mechanism between the flame-generated pressure wave and the combustion wave is discussed in detail.

• 한국식품영양학회지
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• 제7권2호
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• pp.119-123
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• 1994

Aspergillus sp. BY-54 which produced a strong dextran hydrolyzing enzyme was isolated from soil. Using this strain, the optimal cultural conditions, enzyme purification and characterization were studied. The results are as follows : The optimal concentration of dextran as carbon source was l%. and the optimum temperature and the initial pH for enzyme production was 3$0^{\circ}C$, and 7.0, respectively. Dextranase was purified by DEAE-cellulose column chromatography with a linear gradient increase in NaCl. Km value of dextranase was 0.222%, and several glucans containing various types of glucosidic linkages such as DEAE-sephadex, CM-sephadex and sephadex G-100 were almost digested to a large extent with this dextranase. The enzyme was strongly inhibited by sodium fluoride, KMnO4 and p-CMB, while KCN caused 20% of activation.

• 한국표면공학회지
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• 제36권6호
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• pp.430-436
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• 2003

The nanoocrystalline Ni-l5W(at.%) coating electrodeposited on the high carbon steel was oxidized at 700 and $800^{\circ}C$ in air, and the resultant oxidation properties were investigated using XRD, EPMA, TGA and TEM. The oxidation resistance of the coating was not so good that most of the coating was oxidized after oxidation at $800^{\circ}C$ for 5 hrs. The oxidation led to the formation of the outer, thin NiO oxide scale and the inner, porous, rather thick ($NiWO_4$＋NiO) mixed layer containing a bit of $WO_2$. During oxidation, substrate elements such as Fe and Cr diffused outwardly toward the coating, according to the concentration gradient.

• Bulletin of the Korean Chemical Society
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• 제14권6호
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• pp.692-696
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• 1993

Prediction of retention times in transition metal-mandelate and transition metal-tartrate complex systems were studied on the cation exchanger. Plots of k' vs [mandelate] and k' vs [tartrate] were obtained under the condition of a constant competing cation concentration. The equation to predict the retention time of transition metal ion was derived from the ion exchange equilibria. Individual capacity factors (${k_1}',\;{k_2}'$) and stability constants ($K_1,\;K_2$) of the complexes were calculated from the non-linear least square method. Good resolution of the transition metals was predicted by the stepwise equation in the gradient method. The values of retention times from the calculation and the experiment agreed well each other.

• Bulletin of the Korean Chemical Society
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• 제13권4호
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• pp.398-404
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• 1992

We have investigated the kinetics of diffusion-influenced bimolecular reactions in which one reactant has an internal mode, called the gating mode, that activates or deactivates its reactivity intermittently. The rate law and an expression for the time-dependent rate coefficient have been obtained from the general formalism based on the hierarchy of kinetic equations involving reactant distribution functions. The analytic expression obtained for the steady-state reaction rate constant coincides with the one obtained by Szabo et al., who derived the expression by employing the conventional concentration-gradient approach. For the time-dependent reaction rate coefficient, we obtained for the first time an exact analytic expression in the Laplace domain which was then inverted numerically to give the time-domain results.