• Title/Summary/Keyword: Component Effects

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Molecular cloning, Expression and purification of Anthrax toxin from Bacillus anthracis

  • Yoon, Moon-Young
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.323-325
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    • 2002
  • Bacillus Anthracis is the causative agent of anthrax. The major virulence factors are a poly-D glutamic acid capsule and three-protein component exotoxin, which is collectively known as anthrax toxin, protective antigen (PA, 83 kDa), lethal factor (LF, 90 kDa), and edema factor (EF, 89 kDa). These three proteins individually have no known toxic activities, but in combination with PA form two toxins (lethal toxin and edema toxin), causing different pathogenic responses in animals and cultured cells. However, it remains to be elucidated for pathogenic mechanism of anthrax toxin. In this study, we constructed toxin component in bacterial overexpression system and purified the native toxin from Bacillus anthracis delta sterne F32 using FPLC system. Recombinant toxin showed high homogeneity and rapid purification processes. Also, this recombinant toxin was comparable to B. anthracis native toxin in terms of cytotoxic effects on cultured cell lines.

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Effect of Acid Buffering Capacity and Soil Component Remediation of Soil Contaminated with Phenanthrene using Electrokinetic-Fenton Process (산 완충능력과 토양 성분이 동전기-펜톤 공정에 의한 phenanthrene 오염토양 정화에 미치는 영향)

  • Kim, Jung Hwan;Na, So Jeong;Park, Joo Yang;Byun, Young Deog
    • Journal of Soil and Groundwater Environment
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    • v.18 no.1
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    • pp.129-136
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    • 2013
  • This research was conducted to investigate effects of acid buffering capacity and soil component in treatment of phenanthrene using electrokinetic-Fenton process. In Hadong clay of high acid buffering and low iron oxide content, it was difficult to oxidize phenanthrene due to shortage of iron catalyst and scavenger effect of carbonate minerals. The desorbed phenanthrene conductive to Fenton oxidation was transported toward cathode by electroosmotic flow. However, in Youngdong illitic clay, oxidation of phenanthrene near anode readily occurred compared to Hadong clay due to high iron content and low acid buffering capacity.

Modal analysis of cracked cantilever composite beams

  • Kisa, Murat;Arif Gurel, M.
    • Structural Engineering and Mechanics
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    • v.20 no.2
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    • pp.143-160
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    • 2005
  • Modal analysis of cracked cantilever composite beams, made of graphite-fibre reinforced polyamide, is studied. By using the finite element and component mode synthesis methods, a numeric model applicable to investigate the vibration of cracked composite beams is developed. In this new approach, from the crack section, the composite beam separated into two parts coupled by a flexibility matrix taking into account the interaction forces. These forces are derived from the fracture mechanics theory as the inverse of the compliance matrix calculated with the proper stress intensity factors and strain energy release rate expressions. Numerical results are obtained for modal analysis of composite beams with a transverse non-propagating open crack, addressing the effects of the location and depth of the crack, and the volume fraction and orientation of the fibre on the natural frequencies and mode shapes. By means of modal data, the position and dimension of the defect can be found. The results of the study confirmed that presented method is suitable for the vibration analysis of cracked cantilever composite beams. Present technique can be easily extended to composite plates and shells.

A New Approach to the Design of a Fuzzy Sliding Mode Controller for Uncertain Nonlinear Systems

  • Seo, Sam-Jun;Kim, Dong-Sik;Kim, Dong-Won;Yoo, Ji-Yoon;Park, Gwi-Tae
    • 제어로봇시스템학회:학술대회논문집
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    • 2004.08a
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    • pp.646-651
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    • 2004
  • This paper deals with a new adaptive fuzzy sliding mode controller and its application to an inverted pendulum. We propose new method of adaptive fuzzy sliding mode control scheme that the fuzzy logic system is used to approximate the unknown system functions in designing the SMC of uncertain nonlinear systems. The controller's construction and its analysis involve sliding modes. The proposed controller consists of two components. Sliding mode component is employed to eliminate the effects of disturbances, while a fuzzy model component equipped with an adaptation mechanism reduces modeling uncertainties by approximating model uncertainties. To demonstrate its performance, the proposed control algorithm is applied to an inverted pendulum. The results show that both alleviation of chattering and performance are achieved

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Fabrication and feasibility estimation of Micro Engine Component (미세 엔진 운용성 검증 및 요소 기술 개발)

  • Lee, Dae-Hoon;Park, Dae-Eun;Choi, Kwon-Hyoung;Yoon, Joon-Bo;Kwon, Se-Jin;Yoon, Eui-Sik
    • Proceedings of the KSME Conference
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    • 2001.06d
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    • pp.31-36
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    • 2001
  • As a part of micro engine development feasibility estimation was done through fabrication and test of down scaled combustor and MEMS fabricated spark electrode. In an experimental observation of the down scaled combustion phenomena where flame propagation was observed by optical method and pressure change in combustor which gives the information about the reaction generated thermal energy was recorded and analyzed. Optimal combustor scale was derived to be about 2mm considering increased heat loss effect and thermal energy generation capability. Through the fabrication and discharge test of MEMS electrode effects of electrode width and gap was investigated. Electrode was fabricated by thick PR mold and electroplating. From the result discharge voltage characteristic in sub millimeter scale electrode having thickness of $40{\mu}m$ was obtained. From the result base technology for design and fabrication of micro engine was obtained.

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Physicochemical Characteristics and Physiological Functions of ${\beta}-Glucans$ in Barley and Oats (보리, 귀리 ${\beta}-Glucan$의 이화학적 특성과 생리적 기능)

  • Lee, Young-Tack
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.spc1
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    • pp.10-24
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    • 1996
  • [ ($1{\to}3$) ], ($1{\to}4$)-${\beta}$-D-glucans(${\beta}-glucans$) are a major component of the cell walls of grasses as a component of the cereal endosperm and aleurone cell walls. Although ${\beta}-glucans$ exist in all cereals, their concentration is highest in oats and barley. Genetic and environmental differences are found in total ${\beta}-glucan$ content. Both oats and barley ${\beta}-glucans$ have cholesterol-lowering effects. This suggests possible use as food additives. Structural characterization of ${\beta}-glucan$ is important because structure can influence physical and physiological properties. In this review, ${\beta}-glucans$ of barley and oats are discussed in details including structure, chemical and physical properties, and nutritional implications. The use of barley and oat products as well as ${\beta}-glucan$ as a food additive continues to increase. This can provide an additional market for barley and oats, thus increasing the value of the crops.

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Performance of NACA 65-810 Radial Airfoil Impellers (NACA 65-810 반경류 에어포일 임펠러의 성능특성)

  • Kang, Shin-Hyoung;Hu, Shengli
    • The KSFM Journal of Fluid Machinery
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    • v.1 no.1 s.1
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    • pp.24-31
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    • 1998
  • Aerodynamic performance tests and flow measurement were carried out for several radial impellers of NACA 65-810 airfoil. The data base obtained are to be used for verifying the methods of flow analysis and CFD codes. The effects of numbers and span of blades on the performances, efficiency and impeller exit flow are investigated in the present study. The flow rate on the performance curve is proportional to the span of the blade for the same value of fan pressure rise. The magnitude of radial velocity component at the impeller exit gradually decreases from the hub to shroud side. The magnitude of tangential velocity component gradually increases from the hub to shroud side. The way of variations of velocity is the same at the diffuser exit, however, becomes more uniform. The pressure rise performance increases with blade number at the small flow coefficients, however, decreases with the number of blade at the large flow coefficients. This shows that flow guidance in important at the low flow rate and the friction becomes significant at the high flow rate.

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Adaptive Fuzzy Sliding Mode Control for Uncertain Nonlinear Systems

  • Seo, Sam-Jun
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.11 no.1
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    • pp.12-18
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    • 2011
  • This paper deals with a new adaptive fuzzy sliding mode controller and its application to an inverted pendulum. We propose a new method of adaptive fuzzy sliding mode control scheme that the fuzzy logic system is used to approximate the unknown system functions in designing the SMC of uncertain nonlinear systems. The controller's construction and its analysis involve sliding modes. The proposed controller consists of two components. Sliding mode component is employed to eliminate the effects of disturbances, while a fuzzy model component equipped with an adaptation mechanism reduces modeling uncertainties by approximating model uncertainties. To demonstrate its performance, the proposed control algorithm is applied to an inverted pendulum. The results show that both alleviation of chattering and performance are achieved.

A Hydration Model for Blended Concrete utilizing Secondary Cementitious Powders (혼화재를 사용한 콘크리트의 수화모델)

  • Noh Jea Myoung;Byun Keun Joo;Song Ha-Won
    • Proceedings of the Korea Concrete Institute Conference
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    • 2004.05a
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    • pp.140-143
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    • 2004
  • Heat of hydration of concrete under different curing temperatures can be characterized with knowledge of the thermal activity, the heat rate at the reference temperature, and the total heat of hydration of the mixture. The so-called multi-component hydration model incorporates the effect of following variables: cement chemical composition, cement fineness, secondary cementitious powders, mixture proportions, and concrete properties. However, the model does not consider the use of silica fume as a secondary cementitious powder. Therefore, the model that quantifies the heat of hydration due to the use of silica fume is needed. In this thesis, the effects of silica fume on heat of hydration are evaluated and the influence on the heat of hydration are also quantified to be included in the model, so that the analysis using modified multi-component hydration model for silica fume concrete provides more accurate results than normal concrete.

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An Analysis for Gate-source Voltage of GaN HEMT Focused on Mutual Switch Effect in Half-Bridge Structure (GaN HEMT를 사용한 Half-Bridge 구조에서의 스위치 상호작용에 의한 게이트 전압분석)

  • Chae, Hun-Gyu;Kim, Dong-Hee;Kim, Min-Jung;Lee, Byoung Kuk
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.65 no.10
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    • pp.1664-1671
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    • 2016
  • This paper presents the analysis of the gate-source voltage of the gallium nitride high electronic mobility transistor (GaN HEMT) in the half bridge structure focused on the mutual effects of two switching operation. Especially low side gate-source voltage is analyzed mathematically according to the high side switch turn-on and turn-off operation. Moreover, the influence of each gate resistance and parasitic component on the switching characteristic of other side switch is investigated, and the formula, simulation and experimental results are compared with theoretical data.