• Journal of Pharmaceutical Investigation
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• v.40 no.3
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• pp.139-153
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• 2010

Nitrosative stress is defined as pathophysiological conditions that are related to covalent modifications of proteins by nitration/nitrosylation by forms of nitrogen oxide ($NO_x$), leading to DNA damage, ultimately, cell death. This type of stress condition appears to be associated with a number of disease states, including diabetes, inflammation and neurodegenerative diseases. Since these pathological conditions are frequently chronic in nature and, thus, require long-term treatment, changes in pharmacokinetics are likely to affect the therapy. Transporters are membrane proteins that facilitate the movement of substrates, including drugs, across plasma membranes of epithelial / endothelial cells. Since it is now increasingly evident that transporters are pharmacokinetically significant, functional alteration of transporters by this stress condition may have therapeutic relevance. In this review, experimental techniques that are used to study both in vivo and in vitro nitrosative stress are summarized and discussed, along with available literature information on the functional implication of transporters under conditions of nitrosative stress conditions. In the literature, both functional induction and impa irment were apparently present for both drug transporter families [i.e., ATP-binding cassette (ABC) and solute carrier families (SLC)]. Furthermore, a change in the function of a certain transporter appears to have temporal dependency by impairment in the early phase of nitrosative stress and induction thereafter, suggesting that the role of nitrosative stress is complex in terms of functional implications of the transporters. Although the underlying mechanisms for these alterations are not fully understood, protein nitration/nitrosylation appears to be involved in the functional impairment whereas transcript factor(s) activated by nitrosative stress may play a role, at least in part, in functional induction. Interestingly, functional induction under conditions of nitrosative stress has not been observed for SLC transporters while such impairment has been documented for both ABC and SLC transporters. Further investigations appear to be necessary to fully delineate the underlying reasons for these differences on the impact and importance of nitrosative stress conditions.

• Korean Journal of Community Nutrition
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• v.6 no.5
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• pp.715-725
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• 2001

This study was carried to estimate the prevalence of obesity and to investigate the relationship between stress and obesity in children. The subjects were 508 children, who were randomly selected from the fifth and sixth grade at nine elementary schools in Kangnung. The height, body weight, waist and hip circumferences and body fat(%) were measured and the levels of stress were assessed by a questionnaire consisting of the following 3 domains ; 4 items for personal factors, 11 for home, and 18 for school. The prevalence of obesity in male and female children greatly varied by the indices from 2.5% and 1.7% when judged by BMI, 9.5% and 4.0% by obesity index, and 29.7% and 34.4% by body fat(%). The stress scores from personal(47.1%) and school (47.9%) factors were higher than the stress score from home(38.5%). The stress score from personal factors of female children is significantly higher than that of male children, but the stress score from home of female children is significantly lower than that of male children. The personal stress score and the total stress score of obese female children were significantly higher than those of non-obese female children. In male children, the obesity index(%) or body fat(%) have a significant positive correlation with the stress scores from over-expectation of parents, a lack of conversation with family, examinations, discontent about school, teacher's partiality to students, and sexual curiosity. The female children have a significantly positive correlation of obesity index (%) or body fat(%) with stress scores from appearance, quarrels of parents, and inferiority complex to brothers or sisters. These results suggested that counselling and education about not only balanced diet but also the strategies for actively coping with stress are needed to prevent and treat childhood obesity.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.10
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• pp.927-932
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• 2013

The intracranial aneurysm model is extracted based on the Computed Tomography (CT) scan images. Computational fluid dynamics simulations were conducted under both steady and realistic flow conditions in ANSYS-FLUENT. The minimum wall shear stress in the intracranial aneurysm tended to occur in the aneurysmal region. The magnitude of wall shear stress along inner wall of the curvature in the right M1 segment of middle cerebral artery is approximately 20 times higher than that along both the proximal and distal walls. However, the magnitudes of the wall shear stress at the aneurysm region were considerably low. The blood flow has the complex distribution in the aneurysmal region during the systolic period. Complex helical flow patterns are observed inside the aneurysm. Through an analysis of the hemodynamic characteristics, one may predict the rupture of the cerebral aneurysms.

• Interaction and multiscale mechanics
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• v.2 no.2
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• pp.177-187
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• 2009

Machine or structural members subjected to fatigue loading will have a crack initiated during early part of their life. Therefore analysis of members with cracks and other discontinuities is very important. Finite element method has enjoyed widespread use in engineering, but it is not convenient for crack problems as the region very close to crack tip is to be discretized with very fine mesh. However, as the body force method (BFM), requires only the boundary of the discontinuity (crack or hole) to be discretized it is easy versatile technique to analyze such problems. In the present work fundamental solution for concentrated load x + iy acting in the semi-infinite plate at an arbitrary point $z_0=x_0+iy_0$ is considered. These fundamental solutions are in complex form ${\phi}(z)$ and ${\psi}(z)$ (England 1971). These potentials are known as Melan potentials (Ramakrishna 1994). A crack in the semi-infinite plate as shown in Fig. 1 is considered. This crack is divided into number of divisions. By applying pair of body forces on a division, the resultant forces on the remaining 'N'divisions are to be found for which ${\phi}_1(z)$ and ${\psi}_1(z)$ are derived. Body force method is applied to calculate stress intensity factor for crack in semi-infinite plate. Also for the case of crack emanating from circular hole in semi-infinite plate radial stress, hoop stress and shear stress are calculated around the hole and crack. Convergent results are obtained by body force method. These results are compared with FEM results.

• Korean Journal of Health Education and Promotion
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• v.31 no.3
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• pp.73-81
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• 2014

Objectives: This study examined how adolescents' cognitive stress relates to suicidal thought, thereby offering basic data about social and institutional ways to reduce adolescent suicide. Methods: In this study, data from an online survey on adolescents' health conducted by Korea Center for Disease Control(2013, 9th) was used after altering it to fit the purpose of the study. The data was analysed using the complex sampling method and structural equation model(SEM). Results: Whilst adolescents' cognitive stress had positive effects on suicidal thought, problem drinking and cognitive depression, it had a negative effect on physical activity. The structural equation model from cognitive stress, problem drinking, cognitive depression, physical activity influenced suicidal thought. Problem drinking on the other hand had an influence on cognitive depression. Conclusion: These findings suggest that future intervention programs for prevention of adolescents' suicidal thought should also address problem drinking, cognitive depression, and cognitive stress.

• BMB Reports
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• v.40 no.5
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• pp.644-648
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• 2007

Exposure of algae or plants to irradiance from above the light saturation point of photosynthesis is known as high light stress. This high light stress induces various responses including photoinhibition of the photosynthetic apparatus. The degree of photoinhibition could be clearly determined by measuring the parameters such as absorption and fluorescence of chromoproteins. In cyanobacteria and red algae, most of the photosystem (PS) II associated light harvesting is performed by a membrane attached complex called the phycobilisome (PBS). The effects of high intensity light (1000-4000 ${\mu}mol$ photons $m^{-2}s^{-1}$) on excitation energy transfer from PBSs to PS II in a cyanobacterium Spirulina platensis were studied by measuring room temperature PC fluorescence emission spectra. High light (3000 ${\mu}mol$ photons $m^{-2}s^{-1}$) stress had a significant effect on PC fluorescence emission spectra. On the other hand, light stress induced an increase in the ratio of PC fluorescence intensity of PBS indicating that light stress inhibits excitation energy transfer from PBS to PS II. The high light treatment to 3000 ${\mu}mol$ photons $m^{-2}s^{-1}$ caused disappearance of 31.5 kDa linker polypeptide which is known to link PC discs together. In addition we observed the similar decrease in the other polypeptide contents. Our data concludes that the Spirulina cells upon light treatment causes alterations in the phycobiliproteins (PBPs) and affects the energy transfer process within the PBSs.

• The Journal of Korean Academic Society of Nursing Education
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• v.22 no.1
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• pp.14-24
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• 2016

Purpose: The purpose of this study was to identify the impact of academic stress on depression suffered by college nursing students and the moderating effects of academic resilience. Methods: For this survey, 257 students (1st and 2nd year) participated, and it was performed over two weeks in September 2014. In this study, statistical analysis was conducted using SPSS 18.0 for Windows and as a t-test, ANOVA, Pearson correlation, and multiple regression analysis. Results: The students' academic stress had a significant impact on their depression (${\beta}=.279$). The moderating effects of academic resilience did not have a significant impact, which is presumably as a result of there being more complex, nonlinear effects, rather than a simple, linear relationship between academic stress and resilience. Conclusion: It may be incorrect to assume that academic resilience is a moderating variable that lessens academic stress. It is implied that academic resilience can have a significant impact when internal and external support conditions are met, and that the effects can be reinforced when family support, a learning environment, and improved adaptability to life at school are also provided.

• BMB Reports
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• v.42 no.11
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• pp.719-724
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• 2009

Recent studies have revealed that endoplasmic reticulum (ER) disturbance is involved in the pathophysiology of neurodegenerative disorders, contributing to the activation of the ER stress-mediated apoptotic pathway. Therefore, we investigated here the molecular mechanisms underlying the ER-mitochondria axis, focusing on calcium as a potential mediator of cell death signals. Using NT2 cells treated with brefeldin A or tunicamycin, we observed that ER stress induces changes in the mitochondrial function, impairing mitochondrial membrane potential and distressing mitochondrial respiratory chain complex Moreover, stress stimuli at ER level evoked calcium fluxes between ER and mitochondria. Under these conditions, ER stress activated the unfolded protein response by an overexpression of GRP78, and also caspase-4 and-2, both involved upstream of caspase-9. Our findings show that ER and mitochondria interconnection plays a prominent role in the induction of neuronal cell death under particular stress circumstances.

• Journal of the Korean Wood Science and Technology
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• v.34 no.5
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• pp.19-28
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• 2006

There has been considerable research in recent times in light-timber med structures in fires. These structures have included horizontal (floor-like) panels in bending and walls under eccentric and approximately concentric vertical loading. It has been shown that compression properties are the most dominant mechanical properties in affecting structural response of these structures in fire. Compression properties have been obtained by various means as functions of one variable only, temperature. It has always been expected that compression properties would be significantly affected by moisture and stress, as well. However, these variables have been largely ignored to simplify the complex problem of predicting the response of light-timber framed structures in fire. Full-scale experiments on both the panels and walls have demonstrated the high level of significance of moisture and stress for a limited range of conditions. Described in this paper is an overview of these conditions and experiments undertaken to obtain compression properties as a functions of moisture, stress and temperature. The experiments limited temperatures to $20{\sim}100^{\circ}C$. At higher temperatures moisture vaporizes and moisture and stress are less significant. Described also is a creep model for wood at high temperatures.

• International Journal of Automotive Technology
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• v.2 no.1
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• pp.9-16
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• 2001

The lifetime of power train components can be improved dramatically by finding crack initiation points with suitable software tools and optimization of the critical areas. With increasing capacities of computers the prediction of the lifetime for components by numerical methods gets more and more important. This paper discusses some applications of the outstanding fatigue simulation program FEMFAT supporting the assessment of uniaxially and multiaxially loaded components (as well as welding seams and spot joints). The theory applied in FEMFAT differs in some aspects from classical approaches like the nominal stress concept or the local one and can be characterized by the term "influence parameter method". The specimen S/N-curve is locally modified by different influence parameters as stress-gradient to take into account notch effects, mean-stress influence which is quantified by means of a Haigh-diagram, surface roughness and treatments, temperature, technological size, etc. It is possible to consider plastic deformations resulting in mean-stress rearrangements. The dynamic loading of power train components is very often multiaxial, e.g. the stress state at each time is not proportional to one single stress state. Hence, the directions of the principal axes vary with time. We will present the way how such complex load situations can be handled with FEMFAT by the examples of a crank case and a gear box.