• 한국지하수토양환경학회지:지하수토양환경
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• 제11권3호
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• pp.66-77
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• 2006

최근에 적용된 지하수 미생물의 군집구조를 밝히는 분자생물학적 및 생화학적 방법들에 대해서 알아보았고 그 결과로서 지하수의 주요 오염물질에 따른 활성화된 미생물군집들이 무엇인지를 밝힌 연구논문들을 종합하여 정리하였다. PCR에 의한 유전자 증폭기술의 발달로 배양 없이 미생물 종류와 개체군을 파악할 수 있게 되었고 각종 finger-printing 방법 (DGGE, SSCP, RISA, microarray) 과 지방산분석법 (PLFA/FAME)을 이용하여 활성화 된 미생물군집구조를 분석하였으며 FISH 등의 방법으로 특정균의 활성도를 알아본 사례들을 조사하였다. 대표적인 지하수오염물질인 유류성분 (n-alkanes, BTEX, MTBE, ethanol)과 염소계 용매 (TCE, PCE, PCB, CE, carbon tetrachloride, chloro-benzene) 등으로 오염되었을 때 우점하는 지하수 미생물상에 대해 보고된 내용을 포함하였다.

• 한국의류산업학회지
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• 제23권5호
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• pp.559-575
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• 2021

The popularity of K-pop, the development of diverse media and communications, and rapid globalization are increasing consumers' attachment to celebrities. These celebrities, called "Human brand", have a growing impact on consumers. However, research on idol celebrities has been conducted mainly on teenagers. Furthermore, there are limits to the studies on consumers' attachment to idol celebrities who do not advertise specific products and intention to purchase products which are not advertised but related to the idol human brand. Therefore, this study has been conducted on 301 out of 400 adult women in their twenties to forties in Korea to examine the relationship between the characteristics of the idol human brand, attachment to the idol human brand, desire to imitate the idol human brand, desire to identify with the idol human brand and its fandom community, and the effect on purchase intention. For the statistical analysis, SPSS and AMOS were used. The study found that the characteristics of the idol human brand which are trust, professionality, social attractiveness, and physical attractiveness positively influenced attachment to the idol human brand. The attachment to the idol human brand positively affected the imitation desire toward the idol human brand, and the identification desire with the idol human brand, and to identify with its fandom community. Additionally, the desire to imitate and to identify with the idol human brand and its fandom community positively affected the intention. Furthermore, the relationships between all variables were found to have a significant mediating effect.

• 한국물환경학회지
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• 제35권1호
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• pp.19-27
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• 2019

The purpose of this study is to evaluate the water quality and phytoplankton community in Bukhan River which account for 44.4 % of the total inflow into Lake Paldang, using multivariate statistical techniques (i.e., correlation analysis, principal component analysis (PCA)/factor analysis (FA)). Water samples were collected from March to November 2015 and the following parameters measured; water temperature, pH, DO, EC, SS, BOD, Chl-a, COD, TN, $NO_3-N$, $NH_3-N$, TP, DTP, $PO_4-P$, and phytoplankton community. The water quality of the main stream and the tributaries were not significantly different apart from the relatively high concentration of BOD, COD and nutrients recorded in MH. The highest cell density of Stephanodiscus hantzschii and Merismopedia glauca dominated phytoplankton was observed in PD. Based on the correlation analysis, total phytoplankton and cyanophyceae were highly correlated with BOD, COD and nutrients. PCA/FA resulted in four main factors accounting for 82.240 % of the total variance in the water quality dataset. The group of component 1 (TN, DTN, DO, $NO_3-N$, water temperature) and component 2 ($PO_4-P$, T-P, DTP, SS) were classified as nutrient element factor whereas component 3 (Chl-a, COD, BOD, $NH_3-N$, pH) was related to organic substances. Hence, the identification of the main potential environmental pollution factors in Bukhan River will help policy makers make better and more informed decisions on how to improve the water quality.

• 한국콘텐츠학회논문지
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• 제15권8호
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• pp.495-504
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• 2015

본 연구에서는 항공사 승무원이 인식하는 사회적 책임이 조직동일시 및 고객지향성에 미치는 영향을 파악해보고자 하였다. 이론적 고찰을 토대로 두 개의 가설을 설정하였으며, 선행연구를 기초로 설문을 구성하였다. 편의표본추출을 통해 217부의 설문지를 항공사 승무원을 대상으로 회수하였으며, 결측치가 있는 자료를 제외하고 203부를 사용하였다. 사회적 책임은 이론적 고찰을 토대로 경제적 책임, 법적 책임, 윤리적 책임, 자선적 책임으로 구분하였으며 확인요인분석을 통해 신뢰성과 타당성을 확보하였다. AMOS를 활용하여 구조방정식 모형을 이용하여 분석하였으며 실증분석 결과는 아래와 같다. 먼저 항공사 승무원이 인식하는 사회적 책임은 조직동일시에 유의한 정(+)의 영향을 미칠 것이라는 가설 1은 부분 채택되었다. 구체적으로 경제적 책임과 자선적 책임은 조직동일시에 유의한 정(+)의 영향을 미치는 것으로 나타나 가설 1-1과 가설 1-4는 채택되었다. 반면, 법적 책임과 윤리적 책임은 유의한 수준에 미치지 않아 가설 1-2와 가설 1-3은 기각되었다. 영향력의 정도는 자선적 책임이 경제적 책임보다 더 많은 영향을 미치는 것으로 나타나 지역사회에 대한 책임을 중시하고 사회공헌을 위해 적극적으로 노력하는 것이 무엇보다 중요하다고 사료된다. 둘째 조직동일시는 고객지향성에 유의한 정(+)의 영향을 미칠 것이라는 가설 2는 채택되었다. 한계점과 향후 논의방향을 제시하였다.

• Structural Monitoring and Maintenance
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• 제3권1호
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• pp.51-69
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• 2016

Monitoring the performance and estimating the remaining useful life of aging civil infrastructure in the United States has been identified as a major objective in the civil engineering community. Structural health monitoring has emerged as a central tool to fulfill this objective. This paper presents a review of the major structural monitoring programs that have been recently implemented in the United States, focusing on the integrity and performance assessment of large-scale structural systems. Applications where response data from a monitoring program have been used to detect and correct structural deficiencies are highlighted. These applications include (but are not limited to): i) Post-earthquake damage assessment of buildings and bridges; ii) Monitoring of cables vibration in cable-stayed bridges; iii) Evaluation of the effectiveness of technologies for retrofit and seismic protection, such as base isolation systems; and iv) Structural damage assessment of bridges after impact loads resulting from ship collisions. These and many other applications show that a structural health monitoring program is a powerful tool for structural damage and condition assessment, that can be used as part of a comprehensive decision-making process about possible actions that can be undertaken in a large-scale civil infrastructure system after potentially damaging events.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.22-26
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• 2002

There are a number of ways in which environmental microbiology and microbial ecology will benefit from DNA micro array technology. These include community genome arrays, SSU rDNA arrays, environmental functional gene arrays, population biology arrays, and there are clearly more different applications of microarray technology that can be applied to relevant problems in environmental microbiology. Two types of the applications, bacterial identification chip and functional gene detection chip, will be presented. For the bacterial identification chip, a new approach employing random genome fragments that eliminates the disadvantages of traditional DNA-DNA hybridization is proposed to identify and type bacteria based on genomic DNA-DNA similarity. Bacterial genomes are fragmented randomly, and representative fragments are spotted on a glass slide and then hybridized to test genomes. Resulting hybridization profiles are used in statistical procedures to identify test strains. Second, the direct binding version of microarray with a different array design and hybridization scheme is proposed to quantify target genes in environmental samples. Reference DNA was employed to normalize variations in spot size and hybridization. The approach for designing quantitative microarrays and the inferred equation from this study provide a simple and convenient way to estimate the target gene concentration from the hybridization signal ratio.

• Mycobiology
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• 제34권1호
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• pp.7-13
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• 2006

Arbuscular mycorrhizas (AM) have mutualistic symbiosis with plants and thus efforts have been placed on application of these symbiotic relationships to agricultural and environmental fields. In this study, AM fungi were collected from 25 sites growing with 16 host plant species in Korea and cultured with Sorghum bicolor in greenhouse condition. AM fungal spores were extracted and identified using both morphological and molecular methods. Using morphological characters, total 15 morpho-speices were identified. DNA was extracted from single spore of AM fungi and a partial region on 18S rDNA was amplified using nested PCR with AM fungal specific primers AML1/AML2. A total of 36 18S rDNA sequences were analyzed for phylogenetic analysis and 15 groups of AM fungi were identified using both morphological and molecular data of spores. Among the species, 4 species, Archaeospora leptoticha, Scutellospora castanea, S. cerradensis, S. weresubiae were described for the first time in Korea and two species in Glomus and a species in Gigaspora were not identified. Morphological and molecular identification of AM fungal spores in this study would help identify AM fungal community colonizing roots.

• 한국지역지리학회지
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• 제9권3호
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• pp.248-261
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• 2003

본고에서는 오사카의 이꾸노 한인축제의 정성적 분석을 통하여 민족적 정체성이 어떻게 구축되며, 커뮤니티 내외적 이해관계를 매개로 그 과정이 어떻게 형성되는지를 고찰하고자 한다. 여기서는 차이보다는 유사성을 고려함으로써 재조직되는 문화의 잠재성에 특히 주목하고 싶다. 이러한 역동적 상호관련은 특정한 사회논리와 정체성이 겨루어지는 속에 특유하며 비공식적인 공공권이 축제에 의해 제공된다는 것을 시사한다. 따라서 이러한 광범위한 활무대는 특정의 배제와 포섭으로 특징지어진다. 이 연구는 축제를 통해 정체성이 구축되고 끊임없이 협의되는 미시적 수준의 복합적 정체화 과정을 드러낸다.

• Journal of Dairy Science and Biotechnology
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• 제18권1호
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• pp.47-60
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• 2000

Over decades of work, the probiotic research has grown rapidly with a number of new cultures, which is claimed a variety of benefit. However, many of the specific effects attributed to the ingestion of probiotics remain convoluted and scientifically unsubstantiated. Accordingly, the scientific community faces a greater challenge and must objectively seek cause and effect relationships for many potential and currently investigated probiotic species. Rational selection and design of probiotics remains an important challenge and will require a solid information about the physiology and genetics of candidate strains relevant to their intestinal roles, functional activities, and interaction of with other resident micro flora. As far as beneficial culture of lactic acid bacteria (LAB) is concerned, simple, cost-effective, and exact identification of candidate strains is of foremost importance among others. Until recently, the relatedness of bacterial isolates has been determined sorely by testing for one or several phenotyphic markers, using methods such as serotyping, phage-typing, biotyping, and so forth. However, there are problems in the use of many of these phenotype-based methods. In contrast, some of newer molecular typing methods involving the analysis of DNA offer many advantages over traditional techniques. These DNA-based methods have the greater discriminatory power than that of phenotypic procedures. This review focuses on the importance and the basis of molecular typing methods along with some considerations on de-sign and selection of probiotic culture for human consumption.

• Journal of Microbiology and Biotechnology
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• 제24권1호
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• pp.113-118
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• 2014

Environmental microorganisms are emerging as an important source of new enzymes for wide-scale industrial application. In this study, novel phytase genes were identified from a soil microbial community. For this, a function-based screening approach was utilized for the identification of phytase activity in a metagenomic library derived from an agricultural soil. Two novel phytases were identified. Interestingly, one of these phytases is an unusual histidine acid phosphatase family phytase, as the conserved motif of the active site of PhyX possesses an additional amino acid residue. The second phytase belongs to a new type, which is encoded by multiple open reading frames (ORFs) and is different to all phytases known to date, which are encoded by a single ORF.