Browse > Article
http://dx.doi.org/10.4489/MYCO.2006.34.1.007

Molecular Identification of Arbuscular Mycorrhizal Fungal Spores Collected in Korea  

Lee, Jai-Koo (Department of Biology Education, Korea National University of Education)
Park, Sang-Hyeon (Department of Biology Education, Korea National University of Education)
Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
Publication Information
Mycobiology / v.34, no.1, 2006 , pp. 7-13 More about this Journal
Abstract
Arbuscular mycorrhizas (AM) have mutualistic symbiosis with plants and thus efforts have been placed on application of these symbiotic relationships to agricultural and environmental fields. In this study, AM fungi were collected from 25 sites growing with 16 host plant species in Korea and cultured with Sorghum bicolor in greenhouse condition. AM fungal spores were extracted and identified using both morphological and molecular methods. Using morphological characters, total 15 morpho-speices were identified. DNA was extracted from single spore of AM fungi and a partial region on 18S rDNA was amplified using nested PCR with AM fungal specific primers AML1/AML2. A total of 36 18S rDNA sequences were analyzed for phylogenetic analysis and 15 groups of AM fungi were identified using both morphological and molecular data of spores. Among the species, 4 species, Archaeospora leptoticha, Scutellospora castanea, S. cerradensis, S. weresubiae were described for the first time in Korea and two species in Glomus and a species in Gigaspora were not identified. Morphological and molecular identification of AM fungal spores in this study would help identify AM fungal community colonizing roots.
Keywords
Arbuscular mycorrhiza; 18S rDNA; Specific primer;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Allen, M. F. 1991. The ecology of mycorrhizae. Cambridge University Press, Cambridge
2 Morton, J. B. and Benny, G. L. 1990. Revised classification of arbuscular mycorrhizal fungi (Zygomycetes): A new order, Glomales, two new suborders, Glomineae and Gigasporineae, and two new families, Acaulosporaceae and Gigasporaceae, with an emendation of Glomaceae. Mycotaxon 37: 471-492
3 Morton, J. B. and Redecker, D. 2001. Two new families of Glomales, Archaeosporaceae and Paraglomaceae, with two new genera Archaeospora and Paraglomus, based on concordant molecular and morphological characters. Mycologia 93: 181-195   DOI
4 Redecker, D., Morton, J. B. and Bruns, T. D. 2000. Molecular phylogeny of the arbuscular mycorrhizal fungi Glomus sinuosum and Sclerocystis coremioides. Mycologia 92: 282-285   DOI
5 Daniels, B. A. and Skipper, H. A. 1982. Methods for the recovery and quantitative estimation of propagules from soil. Pp. 2935. In: Schenck, N. C. Ed. Methods and principles of mycorrhizal research, American Phytopathological Society, St. Paul, Minn
6 Becker, W. N. and Gerdemann, J. W. 1977. Glomus etunicatus sp. nov. Mycotaxon 6: 29-32
7 Bethenfalvay, G. J. and Yoder, J. F. 1981. The Glycine max-Glomus fasciculatus-Rhizobium japonicum Symbiosis 1. Phosphorus effect on nitrogen fixation and mycorrhizal infection. Physiologia Plantarum 52: 141-145   DOI
8 Dalpe, Y., Koske, R. E. and L., T. L. 1992. Glomus lamellosum sp. nov. a new Glomaceae associated with beach grass. Mycotaxon 43: 289-293
9 Lee, S., Hong, S.-B. and Kim, C.-Y. 2003. Contribution to the checklist of soil-inhabiting fungi in Korea. Mycobiology 31: 918
10 van der Heijden, M. G. A., Klironomos, J. N., Ursie, M., Moutoglis, P., Streitwolf, E. R., Boller, T., Wiemken, A. and Sanders, J. R. 1998. Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature 396: 69-72   DOI   ScienceOn
11 Lee, T. B. 1985. Illustrated flora of Korea. Hyang Moon Sa, Seoul, Korea
12 Schenck, N. C. and Perez, Y. 1990. Manual for the identification of VA mycorrhizal fungi, 3rd edition. Synergistic Publications, Gainesville, Florida
13 Redecker, D., Thierfelder, H., Walker, C. and Werner, D. 1997. Restriction analysis of PCR-amplified internal transcribed spacers of ribosomal DNA as a tool for species identification in different genera of the order Glornales. Appl. Environ. Microbiol. 63: 1756-1761
14 Rokas, A., Williams, B. L., King, N. and Carroll, S. B. 2003. Genome-scale approaches to resolving incongruence in molecular phylogenies. Nature 425: 798-804   DOI   ScienceOn
15 Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol. Biol. Evol. 4: 406-425
16 Schuessler, A., Schwarzott, D. and Walker, C. 2001. A new fungal phylum, the Glomeromycota: evolution and phylogeny. Mycol. Res. 105: 1413-1421   DOI   ScienceOn
17 Smith, S. E. and Read, D. J. 1997. Mycorrhizal symbiosis, 2nd edition. Academic Press, London
18 Thompson, J. D., Higgins, D. G and Gibson, T. J. 1994 CLUSTAL X: Improving the sensitivity of multiple sequence alignment though sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 26: 179182   DOI
19 van Tuinen, D., Jacquot, E., Zhao, B., Gollotte, A. and Gianinazzi, P. V. 1998. Characterization of root colonization profiles by a microcosm community of arbuscular mycorrhizal fungi using 25S rDNA-targeted nested PCR. Mol. Ecol. 7: 879-887   DOI   ScienceOn
20 Walker, C. and Schuessler, A. 2004. Nomenclatural clarifications and new taxa in the Glomeromycota. Mycol. Res. 108: 979982