• Journal of Microbiology and Biotechnology
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• 제30권2호
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• pp.248-258
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• 2020

The vaginal microbiota may be important for pregnancy prognosis because vaginal dysbiosis during pregnancy appears to be related to preterm birth (PTB) or pregnancy loss. Previous reports have indicated that a Lactobacillus-poor microbial flora in the vagina and intrauterine infection by diverse anaerobes ascending from the vagina are associated with undesirable delivery outcomes. However, no research has involved the use of pyrosequencing analysis to examine vaginal microbiota profiles or their potential associations with high-risk pregnancy in Korean women. Vaginal swabs were collected from 500 Korean women for the identification of community state types (CSTs). Of these, 137 samples were further analyzed using a Roche/454 GS Junior pyrosequencer. Three distinct CSTs were identified based on the dominant vaginal microbes: CST I (Lactobacillus crispatus dominated), CST III (Lactobacillus iners dominated), and CST IV (with diverse species of anaerobes). Twelve of the 67 pregnant women had undesirable pregnancy outcomes (four miscarriages and eight PTBs). The dominant microbe in the vaginal microbiota of women who gave birth at full-term was L. crispatus. In contrast, L. iners was the dominant vaginal microbe in women who miscarried. Most (n = 6/8) vaginal microbiota profiles of women who experienced PTB could be classified as CST IV, with diverse bacteria, including anaerobic vaginal species. The present study provides valuable information regarding the characteristics of the vaginal microbiota of Korean women related to high-risk pregnancy. Investigation of the vaginal microbiotic structure in pregnant Korean women is necessary to enable better prediction of adverse pregnancy outcomes.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1185-1192
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• 2012

This study investigated the hydrocarbonoclastic microbial community present on weathered crude oil and their ability to degrade weathered oil in seawater obtained from the Gulf St. Vincent (SA, Australia). Examination of the native seawater communities capable of utilizing hydrocarbon as the sole carbon source identified a maximum recovery of just $6.6{\times}10^1\;CFU/ml$, with these values dramatically increased in the weathered oil, reaching $4.1{\times}10^4\;CFU/ml$. The weathered oil (dominated by > $C_{30}$ fractions; $750,000{\pm}150,000mg/l$) was subject to an 8 week laboratory-based degradation microcosm study. By day 56, the natural inoculums degraded the soluble hydrocarbons (initial concentrations $3,400{\pm}700mg/l$ and $1,700{\pm}340mg/l$ for the control and seawater, respectively) to below detectable levels, and biodegradation of the residual oil reached 62% ($254,000{\pm}40,000mg/l$) and 66% ($285,000{\pm}45,000mg/l$) in the control and seawater sources, respectively. In addition, the residual oil gas chromatogram profiles changed with the presence of short and intermediate hydrocarbon chains. 16S rDNA DGGE sequence analysis revealed species affiliated with the genera Roseobacter, Alteromonas, Yeosuana aromativorans, and Pseudomonas, renowned oil-degrading organisms previously thought to be associated with the environment where the oil contaminated rather than also being present in the contaminating oil. This study highlights the importance of microbiological techniques for isolation and characterisation, coupled with molecular techniques for identification, in understanding the role and function of native oil communities.

• Nutrition Research and Practice
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• 제5권1호
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• pp.40-45
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• 2011

Hemoglobin and zinc protoporphyrin (ZPP) tests are commonly used to screen for iron deficiency, but little research has been done to systematically evaluate the sensitivity and specificity of these two tests. The goal of this study was to evaluate the effectiveness of zinc protoporphyrin/heme (ZPP/H) ratio as a point-of-service screening test for iron deficiency among preschool-aged children by comparing the sensitivity and specificity of hemoglobin, ZPP/H ratio, and serum ferritin (SF). Also completed were assessments for the prevalence of anemia, iron deficiency (ID), and iron deficiency anemia (IDA) with indicators of ferritin models. This study was carried out with 95 children ages 3 to 6 y. Anthropometric measurements were assessed and blood samples were analyzed for hemoglobin, SF, transferrin saturation (TS), and ZPP. Anemia was common and the prevalences of anemia, ID, and IDA were 14.7%, 12.6%, and 5.2%, respectively. The ZPP/H ratio was strongly and significantly correlated with hemoglobin. And ZPP/H ratio was a more sensitive test for ID than hemoglobin or SF measurement, correctly identifying more than twice as many iron-deficient children (sensitivity of 91.7%, compared to 41.7% for hemoglobin and SF). However, ZPP/H ratio had lower specificity (60.2%, compared to 89.1% for hemoglobin or 96.4% for SF) and resulted in the false identification of more subjects who actually were not iron deficient than did hemoglobin or SF. Low hemoglobin concentration is a late-stage indicator of ID, but ZPP/H ratio can detect ID at early stages and can be performed easily at a relatively low cost. Therefore, ZPP/H ratio can serve as a potential screening test for pre-anemic iron deficiency in community pediatric practices.

• 농촌계획
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• 제3권1호
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• pp.54-67
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• 1997

Korean societies have been experiencing the wholesale structural changes in the rapid currents of recent openness, globalization and democratization, which effect much more heavily in rural areas than in urban areas, so rural recomposition works being an important national concern. In order to systematically reconstruct the rural structure, the decision makers, with a four step hierarchy of rural resident-residents group-community-region, should be endowed with the objective judgement on basic elements of resource potentialities under their control. In this process. rational resource evaluation works would be firstly necessiated from expert groups. Based on the view mentioned above, this study principally aimed at developing a rational evaluation framework for rural resources. For that objective, the first step of the study pigeonholed the total resources items identifiable in rural areas from the existing study results, spatial planning and field surveying data. After then, using the formalized classification criteria of resources items, a tentative goal system for rural resources evaluation was proposed and the final one determined through expert-group checking. The results obtained during the study are summarized as follows ; 1. Using the existing examples of resources identification/classification and the basic data list for county-level development planning as the principal reference ones, total rural resources elements were classified into 3 constituent units : land, natural environment and human resources, which correspond to places to work, to play and to live, respectively, as 3 constituent ones of life-supporting space. 2. Three characteristic areal types were adopted to represent the total rural areas : lowland, upland and seashore areas, and also 3 practical use types to represent the objectives of resources evaluation systems : for land use planning, natural conservation policy and village improvement planning. Thus 9 different types of goal system for resources evaluation were developed(each system by 3 areal typesX3 practical use types) 3. Each goal system has 3-tier classification steps from the higher, middle and lower one. The higher and middle steps should contain equally applicable components to all the rural areas, of which allowable number being around 3 and 4 respectively. However the lower step would contain detailed sub-components changeable to areal characteristics of which allowable number being around 7.

• Genomics & Informatics
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• 제9권3호
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• pp.114-120
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• 2011

Chronic inflammation has been implicated as one of the important etiological factors in insulin resistance and type 2 diabetes mellitus (T2DM). To investigate the role of anti-inflammatory cytokines in the development of T2DM, we conducted a case-control study to assess the association between IL4/IL4R polymorphisms and disease risk. We firstly identified single nucleotide poly-morphisms (SNP) at IL4 and IL4RA loci by sequencing the loci in Korean participants. Case-control studies were conducted by genotyping the SNPs in 474 T2DM cases and 470 non-diabetic controls recruited from community-based cohorts. Replication of the associated signals was performed in 1,216 cases and 1,352 controls. We assessed effect of IL4 -IL4RA interaction on T2DM using logistic regression method. The functional relevance of the SNP associated with disease risk was determined using a reporter expression assay. We identified a strong association between the IL4 promoter variant rs2243250 and T2DM risk (OR=0.77; 95% CI, 0.67~0.88; p=$1.65{\times}10^{－4}$ in the meta-analysis). The reporter gene expression assay demonstrated that the presence of rs2243250 might affect the gene expression level with ~1.5-fold allele difference. Our findings contribute to the identification of IL4 as a T2D susceptibility locus, further supporting the role of anti-inflammatory cytokines in T2DM disease development.

• 한국사회복지학
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• 제57권1호
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• pp.61-91
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• 2005

본 연구는 한국의 교육현장과 실천현장을 연결하기 위한 목적의 일환으로 한국의 사회복지실천 현장에서 우리의 사회복지사들이 실제 수행하고 있는 실천활동을 분석해 보기 위한 탐색적 연구이다. 이를 위해, 연구자는 현재 한국의 다양한 사회복지 현장에서 활동하고 있는 사회복지사들(669명의 조사 대상자 중 361명이 응답)이 가장 많이 활용하고 있는 실천기술이 무엇인가를 한국의 실천현장에 뿌리를 두고 경험적으로 조사했다. 활용도가 높은 기술 132개의 목록을 제시했으며 그 기술들 안에 존재하는 주요 기술 축 7개를 탐색적 요인분석을 통해 도출했다. 다음으로 이들 각 기술 축 안에 존재하는 하위 기술군들이 요인분석을 통해 도출되었으며, 이들 하위 기술군을 대표하는 핵심기술지표 42개가 제시되었다. 마지막으로 다양한 실천현장별로 각기 다른 실천기술 축을 확인해 분류하였다. 결과의 함의와 후속 연구를 위한 제언이 제시되었다.

• 생물정신의학
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• 제25권3호
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• pp.72-78
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• 2018

Objectives The high suicide rate has risen as a main concern in South Korea. Given the complexity of the mechanism resulting in complete suicide, studies targeting various populations are needed for broader understanding of its risk factors. This study aims to analyze the factors affecting the suicidal tendency in the population of the elderly living alone in Seoul depending on basic elderly-care services. Methods A total number of 415 people participated in the study. Home-visit interviews were administered by trained interviewers. Suicidal tendency was measured by the Korean version of mini international neuropsychiatric interview (K-MINI). Health-related quality of life was measured by a brief version of the World Health Organization Quality-of-life Scale (WHOQOL-BREF). Alcohol problem was assessed by the Korean version of the alcohol use disorders identification test (AUDIT-K). Among total subjects of 415 interviewees, the actual responses of 408 people were used for the final analysis. Results The result showed that the suicide risk was high in persons isolated from social support [odds ratio (OR) = 4.49], having depression (OR = 14.85), and having low quality of life (OR = 4.39). Conclusions We found that social support, depression and health-related quality of life are associated with suicidal tendency in the elderly living alone on basic services. Our evidence will contribute to suicide prevention policy for the elderly living alone on care services.

• 천문학논총
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• 제20권1호
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• pp.55-62
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• 2005

The First Generation Digitized Sky Survey (DSS-I) is a collection of digitized photographic atlases of the night sky taken from the Palomar Observatory (northen sky) and the Anglo-Australian Observatory (southern sky). DSS-I is widely used by the astronomical community for a number of applications including object cross-identification and astrometry. However, accessing and retrieving the actual images are nontrivial owing to the huge size (> 60 GB) of the dataset. To facilitate retrieval process of DSS-I data for the public, Korean Astronomical Data Center (KADC) developed a web application that provides not only data retrieval but also visualization functions. The web application consists of several modules developed using Java Applet, Jave Servlet, and JaveServer Pages (JSP) technologies. It allows users to retrieve images efficiently in various formats such as FITS, JPEG, GIF, and TIFF, and also offers an interactive visulization tool, ImgViewer, for displaying/analyzing FITS images. To use the web application, users require a Java-enabled web browser.

• 농촌지도와개발
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• 제24권2호
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• pp.127-142
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• 2017

Recently, the rapidly growing new generation of renewable energy projects has emerged as an artificial building that creates a mix of natural environments and disruption to the environment. It serves as a quality inhibitor for rural landscapes and implies the absence of favorable rural landscape. Therefore, the study looked at the color scheme of residents, visitors, and experts in the village, which changed the color of the landscape, and analyzed the color scheme of residents, visitors, and experts. In order to do so, the village was divided into three types and conducted a survey by the general public and experts. In the case of the survey, residents of the three districts (Yeoju, Dam yang, Jeju) were surveyed for 210 residents, and the survey conducted a survey and analysis of 30 people in the area. As a result, both the general public and experts recognized the color change in the energy business, which resulted in the identification of associations and colors of the village. In addition, the recognition and satisfaction of the current situation showed that the education programs for the public were more important than the professionals, as the public show ed low levels of public awareness. Based on this research, we expect to be able to utilize the plan as a guideline for the project of installing solar panels, which is expected to be implemented in the beginning of the project, and can be used as a guideline for the establishment of the solar panels.

• Journal of the Korean Data and Information Science Society
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• 제25권2호
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• pp.373-384
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• 2014

데이터 마이닝이란 대량의 데이터나 복잡한 구조의 데이터들을 정교한 통계분석과 모델링 테크닉을 이용하여 정확히 식별되지 않는 패턴이나 자료간의 상관관계를 밝혀내어 여러 가지 결과를 예측해 내는 통계적 기법이다. 이러한 데이터 마이닝 기법은 금융, 통신, 유통, 의학 등 다양한 분야에 활용되는데, 본 연구에서는 의학 분야에 적용하여 호흡기질환에 영향을 끼치는 요인을 선별하였다. 분석은 2012년도 경상북도 지역사회건강조사에 참여한 사람 중 의사에게서 폐결핵, 천식, 알레르기성 비염을 진단받은 경험이 있는 호흡기질환군과 건강군으로 정리한 자료를 대상으로 하였다. 호흡기질환이 영향을 끼치는 주요인을 선별하기 위해 인공신경망, 로지스틱 회귀모형, 베이지안 네트워크, C5.0, CART 기법을 이용하였다. 공정한 모형 평가를 위해 전체 데이터를 훈련용 데이터와 검증용 데이터로 나누었고, 훈련용 데이터에서 설정된 모형을 검증용 데이터에 적용하여 정확도를 비교하였다. 그 결과 CART가 최적 모형으로 선정되었으며 CART의 의사결정나무를 통하여 우울감 인지 여부, 현재 흡연여부, 스트레스 인지 여부 순으로 호흡기질환에 영향을 주는 것으로 나타났다. 그리고 호흡기질환의 주요인들에 대한 오즈비를 구하여 개별적인 영향력에 대해서도 밝혔다.