• 한국식품과학회지
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• 제34권1호
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• pp.136-139
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• 2002

검정현미에 내포한 특이한 향기성분을 토대로 수입산 또는 국내산인지의 여부를 전자코로 분석하였다. 주성분 분석 결과 전반적으로 수입산과 국내산의 판별이 뚜렷하게 구별 가능하였다. 또한 인공신경망 분석 프로그램을 이용하여 학습시켜 원산지를 모르는 시료를 분석한 결과 국내산 검정현미가 수입산인지 국내산인지를 구별할 수 있었다. E-Nose 5000을 이용한 결과는 69종의 국내산 검정현미를 국내산으로 판별한 경우가 64종으로 92.75%이었고, 13종의 수입산 검정현미를 수입산으로 판별한 경우는 11종으로 92.31%의 높을 확률로 metal oxide sensor로만 구성된 전자코의 판별정도보다 더 정확하게 구별할 수 있었다.

• 수산해양기술연구
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• 제41권3호
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• pp.179-187
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• 2005

This study was conducted in order to the experiment of bottom trawl fisheries in the sea mount of central northern pacific($30^{\circ}-40^{\circ}N$, $170^{\circ}-175^{\circ}E$), during the period of July 1 to August 25, 2004 by commercial trawl ship. Fishing experiment was carried out by bottom trawl, with the codend of 110mm mesh size. The number of 101 test fishing was done in the sea mount of central northern pacific during 56 days and the total catches were 198,593kg as 18 fish species, and CPUE/day and catches/day were 8.3 hours towing time and 3,546kg. Catch ratio according to each fishing ground was higher with the order of B, C, and E, and catches for B was 7,171.0kg during the towing time of 13.2 hours. Main target species were Pseudopentaceros richardsoni and Beryx splendens, there was 14.9% of damage ratio for fishing gears on catching the target fishes inhabiting on the rough sea bottom. Catches by water depth was the order of 300m below, 301-350m, 451-500m and 351-400m. It is thought that there was no problem on the mesh size because most of fishes caught from the experimental fishing was 30cm over.

• 원예과학기술지
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• 제32권5호
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• pp.607-613
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• 2014

Calcium has been associated with improved cold tolerance in many crops. The aim of this study was to investigate the changes in leaf cell $Ca^{2+}$ distribution and cell organelle ultrastructure of loquat (Eriobotrya japonica Lindl.) plants in response to cold stress at $-3^{\circ}C$, using transmission electron microscopy (TEM). Two loquat accessions, Zaozhong 6 (a commercial cultivar) and oakleaf loquat (a wild relative) were used. Cold tolerance, as measured by leaf browning rate, was higher in oakleaf plants, and calcium treatment improved cold tolerance in both species. Cold stress first induced inward transport of $Ca^{2+}$ from the intracellular space. Then, the imported $Ca^{2+}$ was aggregated around the chloroplast membrane, finally entering the chloroplast. This pattern of $Ca^{2+}$ distribution in leaf cells occurred earlier in Zaozhong 6 than in the wild loquat. With increasing time of cold exposure, the chloroplast membranes of Zaozhong 6 leaves were damaged, blurred and even disappeared, while those of wild oakleaf loquat leaves maintained their structure longer. In Zaozhong 6, cold stress induced a clear cavity between poorly structured granal thylakoids and vesicles appearing inside the chloroplast, while in oakleaf leaves cold stress had little effect on the ultrastructure of chloroplasts (although chloroplast membranes looked blurred). Loquat leaves accumulated free calcium ions around chloroplasts in response to cold stress, with earlier calcium accumulation occurring in the cold-sensitive cultivar Zaozhong 6 than in wild oakleaf loquat. These results demonstrate that these two loquat species have differences in both cold tolerance and calcium accumulation dynamics.

• 한국식품위생안전성학회지
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• 제35권1호
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• pp.45-50
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• 2020

본 연구는 원주시 내 대형 초밥 뷔페에서 제공되는 초밥, 회 등 26개 수산물 가공품을 대상으로 DNA 바코드를 분석하여 원재료의 종을 동정하였다. DNA 바코드 증폭을 위하여 미토콘드리아의 16S ribosomal RNA 및 cytochrome c oxidase subunit I 유전자 부위를 증폭하는 프라이머 세트를 이용하여 증폭된 PCR 산물의 염기서열을 분석하였다. 확보한 염기서열은 BLAST Search를 이용하여 미국국립보건원 GenBank에 등록되어있는 생물 종의 염기서열과 비교하여 염기서열 유사도와 매칭 점수를 고려하여 최종 종을 동정하였다. 모니터링 결과 분석 제품의 58%는 제품명과 사용된 원재료가 일치하였지만, 27%에서는 불일치가 관찰되었다. 초메기초밥에는 가이양(Pangasianodon hypophthalmus)이 꽃돔회에는 붉평치(Lampris guttatus)가 사용되었으며, 날치알군함 및 청어알무침에는 열빙어(Mallotus villosus) 알이 사용되었음을 확인하였다. 타코와사비군함 및 오징어간장소스에 사용된 원재료는 주꾸미(Amphioctopus fangsiao) 및 남방주꾸미(Amphioctopus membranaceus)로 각각 동정되었다.

• 수산해양기술연구
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• 제43권4호
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• pp.310-319
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• 2007

To investigate the catches of squid jigging fishery, a series of fishing experiments was conducted in the high seas of the Northwest Pacific($40^{\circ}-43^{\circ}N$, $150^{\circ}-155^{\circ}E$) during the period of 1 August to 22 October 2005 by commercial fishing vessel. The number of 142 test fishing was carried out in the Northwest Pacific during 83days. The total catch were 47,524kg as 4 squid species and CPUE was $8.9kg/line{\cdot}day$. CPUE showed high values in the frontal zone during the survey. Main squid species caught from the experimental fishing were the neon flying squid, Ommastrephes bartrami(96.8%) and the boreopacific gonate squid, Gonatopsis borealis(3.2%). Dorsal mantle length of the neon flying squid were increased by the time and increasing of the hook size. Loss rate of the neon flying squid in the water showed the highest values in 28.2% compare to the others. And the loss rate of the front roller and in the air were 1.1% and 1.0%, respectively.

• Preventive Nutrition and Food Science
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• 제10권4호
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• pp.316-325
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• 2005

The antioxidant potential of a $75\%$ methanolic extract of highbush blueberry (Vaccinium corymbosum L.) and its different fractions was investigated using different reactive oxygen species (ROS), nitric oxide (NO.), metal chelating and lipid peroxidation assays. Methylene chloride and $75\%$ methanol fractions showed equally high activities $(IC_{50} 0.010 mg/mL)$ for hydroxyl radical (HO) scavenging. Higher hydrogen peroxide $(H_2O_2)$ scavenging values were reported for the ethyl acetate and methylene chloride fractions and their $IC_{50}$ values were 0.20 and 0.15 mg/mL, respectively. Nitric oxide (NO.) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activities were higher in ethyl acetate and methylene chloride fractions. Chloroform and water fractions showed higher activities in superoxide $(O_2.)$ scavenging. All fractions showed strong metal chelating capacities compared with the commercial antioxidants tested. The $0.1\%$ ethyl acetate fraction showed notable capacity to suppress lipid peroxidation in both fish oil and linoleic acid. Phenolic content was measured in all the fractions and methanolic extract. Among the fractions, ethyl acetate fraction showed the highest phenolic content.

• 한국미생물·생명공학회지
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• 제42권2호
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• pp.121-130
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• 2014

Bacteria 군집 차이를 이용한 신속한 한국산 및 중국산 김치 원산지 판별 가능성의 검토를 위하여 Terminal Restriction Fragment Length Polymorphism (T-RFLP) 분석법을 적용하였다. T-RFLP 분석은 김치발효에 관여하는 주요 유산균의 빠르고, 재현성 있는 검출에는 효과적이었지만, 종(species) 수준에서의 미생물 확인에는 한계를 가지고 있어 한국산 및 중국산 김치에 특이적으로 존재하는 bacteria의 검출에는 부적합한 것으로 평가되었다. T-RFLP를 적용한 발효과정 중의 한국산 및 중국산 김치에 존재하는 bacteria 군집 천이 분석은 비슷한 양상으로 나타났고, Bacillus 속이 발효 후기까지 검출되었다. 또한 Bacillus 속은 발효 후기에 포자를 형성하는 것으로 확인되었다.

• Journal of Microbiology and Biotechnology
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• 제23권1호
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• pp.76-84
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• 2013

A polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique followed by sequencing of the 16S rDNA fragments eluted from the bands of interest on denaturing gradient gels was used to monitor changes in the bacterial microflora of two commercial kimchi, salted cabbage, and ingredient mix samples during 30 days of fermentation at $4^{\circ}C$ and $10^{\circ}C$. Leuconostoc (Lc.) was the dominant lactic acid bacteria (LAB) over Lactobacillus (Lb.) species at $4^{\circ}C$. Weissella confusa was detected in the ingredient mix and also in kimchi samples throughout fermentation in both samples at $4^{\circ}C$ and $10^{\circ}C$. Lc. gelidum was detected as the dominant LAB at $4^{\circ}C$ in both samples. The temperature affected the LAB profile of kimchi by varing the pH, which was primarily caused by the temperature-dependent competition among different LAB species in kimchi. At $4^{\circ}C$, the sample variations in pH and titratable acidity were more conspicuous owing to the delayed growth of LAB. Temperature affected only initial decreases in pH and initial increases in viable cell counts, but affected both the initial increases and final values of titratable acidity. The initial microflora in the kimchi sample was probably determined by the microflora of the ingredient mix, not by that of the salted cabbage. The microbial distributions in the samples used in this study resembled across the different kimchi samples and the different fermentation temperatures as the numbers of LAB increased and titratable acidity decreased.

• Natural Product Sciences
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• 제13권4호
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• pp.304-310
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• 2007

Saussurea costus (Falc.) Lipchitz syn S. lappa C. B. Clarke (commonly known as 'Kuth') belonging to the family Asteraceae is a well known medicinal plant which finds wide usage in different indigenous systems of medicine of India, China, Korea & Tibet. In different folk medicines the roots of S. costus are used to treat various disorders like ulcer, stomachache, malaria, leprosy, dysentery and toothache. However due to over exploitation, it has become endangered and has become the concern of different governmental bodies in India. The increasing demand of this endangered Himalayan species has resulted in a situation where it is often substituted, knowingly or unknowingly, by other morphologically similar species. Arctium lappa, belonging to the same family, is one such plant that has often been found to be present in the market samples of 'Kuth'. The present study was thus carried out and morphoanatomical characters, physicochemical as well as chemical parameters were developed for proper identification of roots of S. costus and its differenciation from A. lappa as well as authentication of the commercial market samples. The detailed morphoanatomical studies revealed that roots of S. costus can be distinguished from A. lappa on the basis of some important microscopial characters eg. the schizogenous resin ducts observed in roots of S. costus, were absent in A. lappa.. Besides, the HPTLC fingerprint profile showed a distinct band at Rf. 0.72 in S. costus, which was totally absent in A. lappa and a band at $R_f$ 0.64 in A. lappa which was absent in S. costus Chlorogenic acid, used as a chemical marker for HPTLC analysis, was estimated to be 0.077% in S. costus as compared to 0.107% in A. lappa. Thus these detailed pharmacognostical parameters can be successfully used to distinguish between roots of S. costus and A. lappa.

• Mycobiology
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• 제36권3호
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• pp.161-166
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• 2008

The retrotransposon marY1 is a gypsy family retroelement, which is detected ubiquitously within the fungal taxonomic groups in which mushrooms are included. To utilize marY1 as a molecular marker for the DNA fingerprinting of mushrooms, oligonucleotides marY1-LTR-L and marY1-LTR-R were designed on the basis of highly conserved regions from the multiple sequence alignment of 30 marY1 sequences retrieved from a nucleotide sequence database. In accordance with $\underline{Re}trotransposon$ $\underline{M}icrosatellite$ $\underline{A}mplified$ $\underline{P}olymorphism$ (REMAP) fingerprinting methodology, the two oligonucleotides were utilized together with the short sequence repeat primers UBC807 and UBC818 for polymerase chain reaction using templates from different mushroom genomic DNAs. Among the tested oligonucleotides, the marY1-LTR-L and UBC807 primer set yielded the greatest amount of abundance and variation in terms of DNA band numbers and patterns. This method was successfully applied to 10 mushroom species, and the primer set successfully discriminated between different commercial mushroom cultivars of the same strains of 14 Pleurotus ostreatus and 16 P. eryngii. REMAP reproducibility was superior to other popular DNA fingerprinting methodologies including the random amplified polymorphic DNA method.