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http://dx.doi.org/10.3746/jfn.2005.10.4.316

Characterization of Antioxidant Potential of a Methanolic Extract and Its Fractions of Highbush Blueberry (Vaccinium corymbosum L.)  

Senevirathne Mahinda (Department of Food Bioengineering, Cheju National University)
Jeon, You-Jin (Faculty of Applied Marine Science, Cheju National University)
Ha, Jin-Hwan (Department of Food Bioengineering, Cheju National University)
Lee, Chi-Ho (Department of Animal Products Science, Konkuk University)
Cho, Somi-K. (Faculty of Biotechnology, Cheju National University)
Kim, Soo-Hyun (Department of Food Bioengineering, Cheju National University)
Publication Information
Preventive Nutrition and Food Science / v.10, no.4, 2005 , pp. 316-325 More about this Journal
Abstract
The antioxidant potential of a $75\%$ methanolic extract of highbush blueberry (Vaccinium corymbosum L.) and its different fractions was investigated using different reactive oxygen species (ROS), nitric oxide (NO.), metal chelating and lipid peroxidation assays. Methylene chloride and $75\%$ methanol fractions showed equally high activities $(IC_{50} 0.010 mg/mL)$ for hydroxyl radical (HO) scavenging. Higher hydrogen peroxide $(H_2O_2)$ scavenging values were reported for the ethyl acetate and methylene chloride fractions and their $IC_{50}$ values were 0.20 and 0.15 mg/mL, respectively. Nitric oxide (NO.) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activities were higher in ethyl acetate and methylene chloride fractions. Chloroform and water fractions showed higher activities in superoxide $(O_2.)$ scavenging. All fractions showed strong metal chelating capacities compared with the commercial antioxidants tested. The $0.1\%$ ethyl acetate fraction showed notable capacity to suppress lipid peroxidation in both fish oil and linoleic acid. Phenolic content was measured in all the fractions and methanolic extract. Among the fractions, ethyl acetate fraction showed the highest phenolic content.
Keywords
highbush blueberry (Vaccinium corymbosum L.); antioxidant potential; reactive oxygen species scavenging; methanolic extract; total phenolics;
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