Mycobiology

  • 제36권3호
  • /
  • Pages.161-166
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  • 2008
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  • 1229-8093(pISSN)
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  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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Retrotransposon Microsatellite Amplified Polymorphism Strain Fingerprinting Markers Applicable to Various Mushroom Species

  • Le, Quy Vang (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Won, Hyo-Kyung (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Lee, Tae-Soo (Department of Biology, University of Incheon) ;
  • Lee, Chang-Yun (Greenpeace Mushroom Co.) ;
  • Lee, Hyun-Sook (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University) ;
  • Ro, Hyeon-Su (Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University)
  • 발행 : 2008.09.30
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초록

The retrotransposon marY1 is a gypsy family retroelement, which is detected ubiquitously within the fungal taxonomic groups in which mushrooms are included. To utilize marY1 as a molecular marker for the DNA fingerprinting of mushrooms, oligonucleotides marY1-LTR-L and marY1-LTR-R were designed on the basis of highly conserved regions from the multiple sequence alignment of 30 marY1 sequences retrieved from a nucleotide sequence database. In accordance with $\underline{Re}trotransposon$ $\underline{M}icrosatellite$ $\underline{A}mplified$ $\underline{P}olymorphism$ (REMAP) fingerprinting methodology, the two oligonucleotides were utilized together with the short sequence repeat primers UBC807 and UBC818 for polymerase chain reaction using templates from different mushroom genomic DNAs. Among the tested oligonucleotides, the marY1-LTR-L and UBC807 primer set yielded the greatest amount of abundance and variation in terms of DNA band numbers and patterns. This method was successfully applied to 10 mushroom species, and the primer set successfully discriminated between different commercial mushroom cultivars of the same strains of 14 Pleurotus ostreatus and 16 P. eryngii. REMAP reproducibility was superior to other popular DNA fingerprinting methodologies including the random amplified polymorphic DNA method.

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참고문헌

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피인용 문헌

  1. Terminal repeat retrotransposons as DNA markers in fungi pp.0233111X, 2013, https://doi.org/10.1002/jobm.201200453
  2. and the Development of Specific DNA Markers to Identify the Variant Strain vol.42, pp.1, 2014, https://doi.org/10.5941/MYCO.2014.42.1.46
  3. Screening and Characterization of LTR Retrotransposons in the genomic DNA of Pleurotus eryngii vol.42, pp.1, 2014, https://doi.org/10.4489/KJM.2014.42.1.50
  4. vol.42, pp.2, 2014, https://doi.org/10.5941/MYCO.2014.42.2.167
  5. Biology, dynamics, and applications of transposable elements in basidiomycete fungi vol.101, pp.4, 2017, https://doi.org/10.1007/s00253-017-8097-8
  6. Species clarification of oyster mushrooms in China and their DNA barcoding vol.16, pp.3, 2017, https://doi.org/10.1007/s11557-016-1266-9