• Journal of Microbiology and Biotechnology
• /
• v.17 no.4
• /
• pp.547-559
• /
• 2007

Bacillus thuringiensis (Bt) was first described by Berliner [10] when he isolated a Bacillus species from the Mediterranean flour moth, Anagasta kuehniella, and named it after the province Thuringia in Germany where the infected moth was found. Although this was the first description under the name B. thuringiensis, it was not the first isolation. In 1901, a Japanese biologist, Ishiwata Shigetane, discovered a previously undescribed bacterium as the causative agent of a disease afflicting silkworms. Bt was originally considered a risk for silkworm rearing but it has become the heart of microbial insect control. The earliest commercial production began in France in 1938, under the name Sporeine [72]. A resurgence of interest in Bt has been attributed to Edward Steinhaus [105], who obtained a culture in 1942 and attracted attention to the potential of Bt through his subsequent studies. In 1956, T. Angus [3] demonstrated that the crystalline protein inclusions formed in the course of sporulation were responsible for the insecticidal action of Bt. By the early 1980's, Gonzalez et al. [48] revealed that the genes coding for crystal proteins were localized on transmissible plasmids, using a plasmid curing technique, and Schnepf and Whiteley [103] first cloned and characterized the genes coding for crystal proteins that had toxicity to larvae of the tobacco hornworm, from plasmid DNA of Bt subsp. kurstaki HD-1. This first cloning was followed quickly by the cloning of many other cry genes and eventually led to the development of Bt transgenic plants. In the 1980s, several scientists successively demonstrated that plants can be genetically engineered, and finally, Bt cotton reached the market in 1996 [104].

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.50 no.4
• /
• pp.437-446
• /
• 2017

To evaluate the consequences of possible fisheries regulations of anchovy Engraulis japonicus in the Korea Strait, we developed and applied a simulation-based yield-per-recruit (Y/R) model that considered temperature-dependent growth and size-dependent mortality, covering the egg to adult stages. We projected changes in commercial yield and egg production of anchovy with respect to varying biological reference points of 1) the instantaneous fishing mortality, 2) the minimum fork length of anchovy allowed to catch for protecting smaller anchovy ($L_{c,min}$), and 3) the maximum fork length allowed to catch for protecting bigger anchovy ($L_{c,max}$). Our Y/R model showed that the anchovy yield will be maximized at ca. $1.4{\times}10^6tons$ when $L_{c,min}$ ranges between 42-60 mm or at ca. $0.8{\times}10^6tons$ when $L_{c,max}$ ranges from 88-160 mm. At $L_{c,min}=30mm$, the present minimum length of catch, our simulations indicated that the anchovy yield can reach a maximum of $1.2{\times}10^6tons$ in the long-term when the present fishing effort, which annually yields ca. $0.2{\times}10^6tons$ of anchovy, can be increased by a factor of 28. We expect that our simulation-based Y/R model can be applied to other commercially-important small pelagic species in which the traditional Beverton-Holt Y/R model is difficult to apply.

• Research in Plant Disease
• /
• v.22 no.3
• /
• pp.133-144
• /
• 2016

Powdery mildew (Podosphaera xanthii) causes severe damage to pumpkin crops grown in open fields and plastic house. Initially, we used ten accessions of pumpkin species; Cucurbita pepo (3), C. maxima (2), C. moschata (2), and C. argyrosperma (3) to study the disease progress in different stages of seedling development. Commercial pumpkin varieties were used as susceptible host for inoculum management and young seedlings were inoculated by dusting powdery mildew spores on the cotyledons, 1-true-leaf and 2-true-leaf seedling stages. Based on initial study, we further evaluated 218 pumpkinaccessions for their resistance to powdery mildew in different seedling stages under plastic house. Area under disease progress curve (AUDPC) and relative AUDPC (rAUDPC) was higher in cotyledonary and 1-true-leaf than 2-true-leaf stage. Seedlings at cotyledons and 1-true-leaf seedling stage displayed more susceptibility to powdery mildew. Based on evaluation of 2-true-leaf stage, IT 110859 and IT 278459 from C. pepo and C. argyrosperma identified as resistant (<0.2). Of the 228 pumpkin accessions, 21 (9.2%) pumpkin accessions consisting of C. pepo (2), C. maxima (5), C. moschata (13), and C. argyrosperma (1) exhibited intermediate resistance (<0.4) to powdery mildew and these accessions are useful to growers for its rational management.

• Journal of Biosystems Engineering
• /
• v.30 no.2 s.109
• /
• pp.121-126
• /
• 2005

In this study, a commercial electronic nose system was used to detect contamination of Salmonella bacteria. Odors from growth media contaminated with Salmonella typhimurium, Salmonella enteritidis, or Escherichia coli were collected and analyzed to evaluate a possibility of rapid detection of pathogen. Odor chromatograph showed that S. typhimurium, S. enteritidis, and E. coli had 7,6, and 9 main peaks, respectively. Retention time and intensity of the peaks were distinct for different bacteria species. Principal component analysis (PCA) were also performed to clarify odor differences. Analysis results showed that the odors for uncontaminated growth medium were differently grouped from the odors of contaminated one. The odor from the bacteria growth identified with two principal components, PC 1 and PC2. In PCA figures, odor groups were moved from left to right of PC 1 with elapse of the bacteria growth time. The electronic nose system could detect odors of S. typhimurium, S. enteritidis, E. coli when their concentration were $1.85\times10^6\;cfu/g,\;2.25\times10^6\;cfu/g,\;and\;1.8\times10^5 cfu/g$, respectively.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.46 no.4
• /
• pp.430-440
• /
• 2010

To investigate the catches of jack mackerel fishery, a series of fishing experiments was conducted in the high seas of the southeastern Pacific Ocean ($30^{\circ}S-38^{\circ}S,\;82^{\circ}W-95^{\circ}W$) during the period of 9 August to 18 December, 2003 by commercial fishing vessel and research vessel. The number of 205 tows fishing was carried out in the southeastern Pacific Ocean. The total catch was 1,988 ton and CPUE was 2.4 ton/hour. CPUE showed high values in the frontal zone during the survey. Main target species caught from the experimental fishing were the jack mackerel, Trachurus murphyi (98.1%) and chub mackerel, Scomber japonicus (1.9%) as bycatch. Body length of the jack mackerel was different between female and male. The high mean catch per unit effort of jack mackerel was showed when the fishing ground of jack mackerel fishery was over the $110^{\circ}W$ in the southeastern Pacific Ocean. But the fluctuation of the catch per unit effort in the western part of fishing ground was not matched with those year. Reliable physical and oceanographical information will be useful for the efficiency of fishing activity. According to the result of monthly movement of center of fishing ground, the fishing activity of jack mackerel fishery was performed northward in the southeastern Pacific Ocean as time passes.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.30 no.1
• /
• pp.114-123
• /
• 2002

A supersonic rocket plum flowfield of kerosene/liquid-oxygen based propulsion system has been analysed using the Reynolds-averaged Navier-Stokes equations coupled with a 9-species 14-reaction finite-chemistry model. The result were compared with chemically frozen flow solution to investigate the effect of finite-rate chemistry on the plume flowfield. The computations were performed using a commercial CFD software, FLUENT 5. The finite-rate chemistry solution exhibited higher temperature caused by the reactions within the nozzle. All the chemical reactions within the plum were dominated only in the shear layer and behind the barrel shock reflection region where the temperatures are high and the effect of finite-rate chemical reactions on the flowfield was found to be insignificant. However, the present plume computation including the finite-rate chemical reaction within the plume has revealed major reactions occurring in the plum and their reaction mechanisms.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.16 no.1
• /
• pp.37-42
• /
• 1980

The purpose of the present study is to find the color induced maximum gathering rate and to observed'the trend of the - gathering rate by using two species of commercial fishes: rock bream, Oplegnathus fasciatus (Temminet et Schlegel) and 'grass puffer, Fugu niphobles (Jordan et Snyder). An experimental tank($360L{\times}50W{\times}55H cm$) was set up in a dark room. An illumination system was attached to the two end of tank to fix horizontal light intensity by co~bination c' one light bulb(20W) and four filters (red, blue, yellow, white) and the five regulating filters in order to fix light intensity. During the experiment water depth was maintained 50 cm lever in the tank. The tank was marked into six longitudinal sections each being 60 em long to observe the distribution of fish. The fish were acclimatized in dark condition for 40 minutes prior to the main experiment. Upon turning on the light, the number of fish in each section was counted 40 times every 30 seconds, and the gathering rates ,were obtain from the average number of fish in each secion. The color induced maximum gathering rate of rock bream appeared to be red, blue yellow and white color orderly.However, that of grass puffer appeared to be blue, white, yellow and red color orderly. Trend of the gathering rate in illumination time showed the remarkable fluctuation in the rock bream and little difference at the two color light sources. However, trend of the gathering rate in illumination time showed the little fluctuation in grass puffer and much difference at the two color light sources.

• Journal of Veterinary Clinics
• /
• v.20 no.3
• /
• pp.357-363
• /
• 2003

These studies were carried out to develop non-contact thermometer to take easily the body temperature of domestic animals instead of taking rectal temperature. For the studies, 86 cattle, 57 horses, 72 pigs, 43 goats, and 42 dogs were used and body parts as neck, flank, axilla, lateral abdomen, gluteus, inguinal region, or jugular groove were chosen for taking temperature according to different species. Two types of commercial non-contact thermometers were used to take the temperature of certain body part and at the same time the rectal temperature using digital thermo-meter was taken to compare the difference of temperature between rectum and certain body part. The difference of mean temperature in cattle between rectum and axilla and flank were 0.52 and $2.41^{\circ}C$, respectively, using non-contact thermometer I, whereas $3.02^{\circ}C$ between rectum and flank using thermometer II. The difference of mean temperature in horses between rectum and axilla, gluteus, and jugular groove were 0.52, 1.49, and $0.26^{\circ}C$, respectively, using thermometer I, whereas 2.28 ane $0.92^{\circ}C$ between rectum and gluteus or jugular groove using thermometer II. The difference of mean temperature in swine between rectum and flank, inguinal region, and neck were 1.23, 0.21, and $0.8^{\circ}C$, respectively, using thermometer I, whereas 1.42, 0.711, and $1.25^{\circ}C$ using thermometer II The difference of mean temperature in goats between rectum and lateral abdomen and inguinal region were 1.02 and $0.12^{\circ}C$, respectively, using thermometer I, whereas 1.96 and $1.01^{\circ}C$ using thermometer II. The difference of mean temperature in dogs between rectum and lateral abdomen, inguinal region, and neck were 3.26, 0.24, and $2.37^{\circ}C$, respectively, using thermometer I, whereas 3.45, 0.56, and $2.61^{\circ}C$ using thermometer II.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.1
• /
• pp.231-235
• /
• 2014

Rh doped Ni and Co catalysts, Rh-M/$CeO_2$(20 wt %)-$Al_2O_3$ (0.2 wt % of Rh; M = Ni or Co, 20 wt %) were synthesized to produce hydrogen via autothermal reforming (ATR) of commercial gasoline at $700^{\circ}C$ under the conditions of a S/C ratio of 2.0, an O/C ratio of 0.84, and a gas hourly space velocity (GHSV) of $20,000h^{-1}$. The Rh-Ni/$CeO_2$(20 wt %)-$Al_2O_3$ catalyst (1) exhibited excellent activities, with $H_2$ and ($H_2$+CO) yields of 2.04 and 2.58 mol/mol C, respectively. In addition, this catalyst proved to be highly stable over 100 h without catalyst deactivation, as evidenced by energy dispersive spectroscopy (EDX) and elemental analyses. Compared to 1, Rh-Co/$CeO_2$(20 wt %)-$Al_2O_3$ catalyst (2) exhibited relatively low stability, and its activity decreased after 57 h. In line with this observation, elemental analyses confirmed that nearly no carbon species were formed at 1 while carbon deposits (10 wt %) were found at 2 following the reaction, which suggests that carbon coking is the main process for catalyst deactivation.

• Mycobiology
• /
• v.42 no.4
• /
• pp.361-367
• /
• 2014

Makgeolli, also known as Takju, is a non-filtered traditional Korean alcoholic beverage that contains various floating matter, including yeast cells, which contributes to its high physiological functionality. In the present study, we assessed the levels of ${\beta}$-glucan and glutathione in various yeast strains isolated from traditional Korean Nuruk and selected a ${\beta}$-glucan- and glutathione-rich yeast strain to add value to Makgeolli by enhancing its physiological functionality through increased levels of these compounds. Yeast ${\beta}$-glucan levels ranged from 6.26% to 32.69% (dry basis) and were strongly species-dependent. Dried Saccharomyces cerevisiae isolated from Nuruk contained $25.53{\mu}g/mg$ glutathione, $0.70{\mu}g/mg$ oxidized glutathione, and $11.69{\mu}g/g$ and $47.85{\mu}g/g$ spermidine and L-ornithine monohydrochloride, respectively. To produce functional Makgeolli, a ${\beta}$-glucan- and glutathione-rich yeast strain was selected in a screening analysis. Makgeolli fermented with the selected yeast strain contained higher ${\beta}$-glucan and glutathione levels than commercial Makgeolli. Using the selected yeast strain to produce Makgeolli with high ${\beta}$-glucan and glutathione content may enable the production of functional Makgeolli.