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Antimicrobial Efficacy of Penicillium amestolkiae elv609 Extract Treated Cotton Fabric for Diabetic Wound Care

  • Rozman, Nur Amiera Syuhada Binti;Hamin, Nurhanis Syafiqah Binti Mohd Nor;Ring, Leong Chean;Nee, Tan Wen;Mustapha, Mahfuzah Binti;Yenn, Tong Woei
    • Mycobiology
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    • v.45 no.3
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    • pp.178-183
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    • 2017
  • Diabetes mellitus is a chronic disorder which affects millions of population worldwide. Global estimates published in 2010 reported the world diabetic prevalence as 6.4%, affecting 285 million adults. Foot ulceration and wound infection are major forms of disabilities arising from diabetic diseases. This study was aimed to develop a natural antimicrobial finishing on medical grade textile that meets American Association of Textiles Chemists and Colorists (AATCC) standard. The textile samples were finished with the ethanolic extract of Penicillium amestolkiae elv609, an endophytic fungus isolated from Orthosiphon stamineus Benth (common name: cat's whiskers). Endophyte is defined as microorganism that reside in the living plant tissue, without causing apparent disease symptom to the host. The antimicrobial efficacy of the ethanolic extract of P. minioluteum was tested on clinical pathogens isolated from diabetic wound. The extract exhibited significant inhibitory activity against 4 bacteria and 1 yeast with the minimal inhibitory concentration ranged from 6.25 to 12.5 mg/mL. The results indicate different susceptibility levels of the test microorganism to the ethanolic extract. However, the killing activity of the extract was concentration-dependent. The finished medical textile showed excellent antimicrobial efficacy on AATCC test assays. All the microbial cultures treated with the textile sample displayed a growth reduction of 99.9% on Hoheinstein Challenge Test. The wash durability of the finished textile was found good even after 50 washes with commercial detergent. Besides, the gas chromatography mass spectrometry analysis showed that 6-octadecenoic acid and diethyl phthalate were the main bioactive constituents of the extract. In conclusion, the developed medical textile showed good antimicrobial efficacy on laboratory tests. This work can be extended to in vivo trials for developing healthcare textile products for antimicrobial applications.

A Study on Ventilation Effects on Smoke Behavior in Rescue Station for Tunnel Fires (철도터널 화재시 구난역 내의 연기거동에 미치는 배연효과에 관한 연구)

  • Jang, Won-Cheol;Kim, Dong-Woon;Lee, Seong-Hyuk;Ryou, Hong-Sun
    • Journal of the Korean Society for Railway
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    • v.11 no.3
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    • pp.294-299
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    • 2008
  • The present study investigates the ventilation effects on smoke spreading with the rescue stations. Experiments for tunnel fires were carried out for n-heptane pool em at different fire locations, and the heat release rates (HRR) were obtained by addition, using the commercial code (FLUENT), the present article presents numerical results for smoke behavior in railway tunnels with rescue station, and it uses the MVHS (Modified Volumetric Heat Source) model for estimation of combustion products resulting from the fire source determined from the HRR measurement. As a result, it is found that smoke propagation is prevented successfully by the fire doors located inside the cross-passages and especially, the smoke behavior in the accident tunnel can be controlled through the ventilation system because of substantial change in smoke flow direction in the cross-passages.

Saponin Analysis and Red Ginseng Production using the Simplified Method of Korean Ginseng (Panax ginseng C.A.Meyer) (고려인삼(Panax ginseng C.A.Meyer)의 간이법에 의한 홍삼제조 및 사포닌 성분분석)

  • In Jun-Gyo;Kim Eun-Jeong;Lee Bum-Soo;Park Myung-Han;Yang Deok-Chun
    • Korean Journal of Plant Resources
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    • v.19 no.1
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    • pp.133-138
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    • 2006
  • In order to enhance the components of bioactive ginsenosides and the manufacturing process of red ginseng, we developed the simplified method for red ginseng production. The red ginseng extract was prepared from red ginseng produced with the simplified method, and the production rate of extract ($62^{\circ}$ brix) was more than 60%. The ginsenosides of red ginseng were purified and analyzed by HPLC using ELSD. Ginsenoside-$Rg_3,\;Rh_2$ and $Rh_1$, specific artifacts found only in red ginseng, were detected by HPLC. Especially, contents of ginsenoside-$Rg_3$ and Rh1 were detected high than two times in red ginseng produced the simplified method compared to commercial products.

Destruction and Removal of PCBs in Waste Transformer Oil by a Chemical Dechlorination Process

  • Ryoo, Keon-Sang;Byun, Sang-Hyuk;Choi, Jong-Ha;Hong, Yong-Pyo;Ryu, Young-Tae;Song, Jae-Seol;Lee, Dong-Suk;Lee, Hwa-Sung
    • Bulletin of the Korean Chemical Society
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    • v.28 no.4
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    • pp.520-528
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    • 2007
  • A practical and efficient disposal of PCBs (polychlorinated biphenyls) in waste transformer oil by a chemical dechlorination process has been reported. The transformer oil containing commercial PCB mixtures (Aroclor 1242, 1254 and 1260) was treated by the required amounts of PEG 600 (polyethylene glycol 600), potassium hydroxide (KOH) and aluminum (Al), along with different reaction temperatures and times. The reaction of PEG with PCBs under basic condition produces arylpolyglycols, the products of nucleophilic aromatic substitution. The relative efficiencies of PCB treatment process were assessed in terms of destruction and removal efficiency (DRE, %). Under the experimental conditions of PEG600/KOH/Al/100 oC/2hr, average DRE of PCBs was approximately 78%, showing completely removal of PCBs containing 7-9 chlorines on two rings of biphenyl which appear later than PCB no. 183 (2,2',3,4,4',5',6-heptaCB) in retention time of GC/ECD. However, when increasing the reaction temperature and time to 150 oC and 240 min, average DRE of PCBs including the most toxic PCBs (PCB no. 77, 105, 118, 123 and 169) in PCB family reached 99.99% or better, with the exception of PCB no. 5 and 8 (2,3-diCB and 2,4'-diCB). In studying the reaction of PEG with PCBs, it confirmed that the process led to less chlorinated PCBs through a stepwise process with the successive elimination of chlorines. The process also permits complete recovery of treated transformer oil through simple segregating procedures.

Effect of Manufacturing Process on Electrochemical Properties of CP-Ti and Ti-6Al-4V Alloys (CP-Ti 및 Ti-6Al-4V 합금의 전기화학적 특성에 미치는 제조공정의 영향)

  • Kim, K.T.;Cho, H.W.;Chang, H.Y.;Kim, Y.S.
    • Corrosion Science and Technology
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    • v.17 no.1
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    • pp.20-29
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    • 2018
  • Ti and its alloys show the excellent corrosion resistance to chloride environments, but they show less corrosion resistance in HCl, $H_2SO_4$, NaOH, $H_3PO_4$, and especially HF environments at high temperature and concentration. In this study, we used the commercially pure titanium and Ti-6Al-4V alloy, and evaluated the effect of the manufacturing process on the electrochemical properties. We used commercial products of rolled and forged materials, and made additive manufactured materials by DMT (Directed Metal Tooling) method. We annealed each specimen at $760^{\circ}C$ for one hour and then air cooled. We performed anodic polarization test, AC impedance measurement, and Mott-Schottky plot to evaluate the electrochemical properties. Despite of the difference of its microstructure of CP-Ti and Ti-6Al-4V alloys by the manufacturing process, the anodic polarization behavior was similar in 20% sulfuric acid. However, the addition of 0.1% hydrofluoric acid degraded the electrochemical properties. Among three kinds of the manufacturing process, the electrochemical properties of additive manufactured CP-Ti, and Ti-6Al-4V alloys were the lowest. It is noted that the test materials showed a Warburg impedance in HF acid environments.

Effect of Oxide Particles Addition to Powder Coating on Corrosion Resistance of Steel Used as Marine Equipments (조선·해양 기자재용 강재의 내식성에 미치는 분체도장 중 산화물 첨가의 영향)

  • Park, Jin-seong;Ryu, Seung Min;Jeong, Yeong Jae;Kim, Sung Jin
    • Corrosion Science and Technology
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    • v.19 no.2
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    • pp.100-107
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    • 2020
  • The demand for powder-coated steel used in the marine industry is increasing owing to their superior corrosion resistance. However, the powder coatings used in commercial products can deteriorate easily by the penetration of brine. In an attempt to suppress brine penetration into the powder coating and significantly increase the corrosion resistance, three types of oxide particles were added to the coating. Electrochemical impedance spectroscopy tests in 3.5% NaCl solution were performed to evaluate the corrosion behaviors of the powder coating with oxide particles. The results showed that the addition of SiO2 particles to a powder coating severely decreased the corrosion resistance due to the easy detachment of agglomerated SiO2 particles with a coarse size from the coating layer. In contrast, the TiO2 and SnO2-added coatings showed better corrosion resistance, and the TiO2-added coating performed best in the test conducted at room temperature. However, conflicting results were obtained from tests conducted at a higher temperature, which may be attributed to the effective suppression of brine penetration by the fine SnO2 particles uniformly distributed in the coating.

Skeletal Muscle Troponin I (TnI) in Animal Fat Tissues to Be Used as Biomarker for the Identification of Fat Adulteration

  • Park, Bong-Sup;Oh, Young-Kyoung;Kim, Min-Jin;Shim, Won-Bo
    • Food Science of Animal Resources
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    • v.34 no.6
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    • pp.822-828
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    • 2014
  • In this study, the existence of skeletal muscle troponin I (smTnI), well-known as a muscle protein in fat tissues, and the utilization of smTnI as a biomarker for the identification of fat adulteration were investigated. A commercial antibody (ab97427) specific to all of animals smTnI was used in this study. Fat and meat samples (cooked and non-cooked) of pork and beef, and chicken considered as representative meats were well minced and extracted by heating and non-heating methods, and the extracts from fat and meat tissues were probed by the antibody used in both enzyme-linked immunosorbent assay (ELISA) and immunoblot. The antibody exhibited a strong reaction to all meat and fat extracts in ELISA test. On the other hand, the results of immunoblot analsis revealed a 23 kDa high intensity band corresponding to the molecular weight of smTnI (23786 Da). These results demonstrate that the existence of smTnI in all animal fat tissues. Since there are monoclonal antibodies specific to each species smTnI, smTnI in fat tissues could be used as a biomarker to identify or determine animal species adulterated in meat products. Therefore, an analytical method to identify fraudulent fat adulteration can be developed with an antibody specific to each species smTnI.

Rapid High Performance Liquid Chromatographic Quantification of Major Isoflavones in Soybeans and Soybean Pastes

  • Kim, Won-Chan;Kwon, Soon-Ho;Rhee, In-Koo;Hur, Jong-Moon;Jeong, Hyun-Hee;Choi, Sun-Ha;Lee, Kyung-Bok;Kang, Young-Hwa;Song, Kyung-Sik
    • Food Science and Biotechnology
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    • v.15 no.1
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    • pp.24-27
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    • 2006
  • A simple HPLC quantification method was developed for genistein, genistin, daidzein, and daidzin in soybeans and soybean products. The procedure used a $4.6{\times}100\;mm$ $Chromolith^{(R)}$ RP-18e column with a mobile phase of 1% HOAc in 20% MeOH to 1% HOAc in 80% MeOH for 10 min. The injection volume was $2\;{\mu}L$ at a flow rate of 2 mL/min. Detection was carried out under UV at 254 nm. Under these conditions, the major isoflavones daidzein, daidzin, genistein, and genistin in soybean and soybean pastes were eluted within 7 min with baseline separation. Optimal extraction of the above four major isoflavones was achieved when 40 g of soybean or soybean paste was refluxed in 100 mL of 95% ethanol for 2 hr. Ten different soybean cultivars and nine commercial soybean pastes were analyzed by this method. The total isoflavone content was highest in the cultivar Somyung ($2,497\;{\mu}g/g$ dry weight). The isoflavone content in soybean pastes varied widely from manufacturer to manufacturer (an almost five-fold difference between the highest and lowest values). Such variations were presumably due to differences in fermentation conditions, type of soybeans used, and levels of such additives as starch and salt.

Hierarchical 5A Zeolite-Containing Carbon Molecular Sieve Membranes for O2/N2 Separation (산소/질소 분리를 위한 다층구조 제올라이트 5A를 함유한 탄소분자체 분리막 제조)

  • Li, Wen;Chuah, Chong Yang;Bae, Tae-Hyun
    • Membrane Journal
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    • v.30 no.4
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    • pp.260-268
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    • 2020
  • Mixed-matrix carbon molecular sieve membranes containing conventional and hierarchically structured 5A were synthesized for application in oxygen (O2)/nitrogen (N2) separation. In general, incorporating 5A fillers into porous carbon matrices dramatically increased the permeability of the membrane with a marginal decrease in selectivity, resulting in very attractive O2/N2 separation performances. Hierarchical zeolite 5A, which contains both microporous and mesoporous domains, improved the separation performance further, indicating that the mesopores in the zeolite can serve as an additional path for rapid gas diffusion without sacrificing O2/N2 selectivity substantially. This facile strategy successfully and cost-effectively pushed the performance close to the Robeson upper bound. It produced high performance membranes based on Matrimid® 5218 polyimide and zeolite 5A, which are inexpensive commercial products.

A Study on the Development of Fashion Accessary Product made with Korean Traditional Paper Hanji II - Focusing on analysis of the related references - (전통 한지를 활용한 패션 액세서리 상품 개발 II - 관련문헌 분석을 중심으로 -)

  • Shim, Joon-Young;Kim, Yong-Sook
    • Korean Journal of Human Ecology
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    • v.15 no.5
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    • pp.803-809
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    • 2006
  • Korean traditional paper 'Hanji' made from the bark of the paper mulberry tree is a good handicraft material because of its high viscosity, durability, dyeability, toughness, flexibility, plasticity, and manipulability. Hanji has been used as a textile material such as cotton wool for protection and keeping us warm from cold weather. However, Hanji has many limitations, while other textile materials have many advantages of such as washability, being sunproof, and fast coloring. The purpose of this study is to review physical properties, formation ability, and dyeability of Hanji as a material of fashion accessary. The contents of this study are composed of 5 parts: 1. To introduce the necessities of this study, 2. To review a historical background of Hanji and to identify its physical characteristics as a fashion accessary material, 3. To review and to compare techniques such as bonding, quilting, knitting, creasing and holding, twisting, folding & braiding, paper pasting, coloring & cutting, and outwashing(?) in making Hanji fashion accessaries, 4. To review dyeing and finishing techniques to increase commercial value, 5. To identify the best fashion accessary materials made of Hanji. The most important characteristics of Hanji as a fashion accessary material are its toughness in a wet state, fast color fixing, and flexibility. Especially weaving and knitting are considered as the most desirable techniques to make fashion accessary products stronger and more practical.

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