• Journal of Microbiology
• /
• v.42 no.4
• /
• pp.305-314
• /
• 2004

An exopolysaccharide-producing microalgal dinoflagellate was isolated from a red-tide bloom and des­ignated strain KG03. A bacteria-free culture of strain KG03 was achieved using a modified wash with phototaxis and antibiotic treatment. Combined treatment with neomycin and cephalosporin was the most effective for eliminating the bacteria associated with the microalgae. Strain KG03 was identified as Gyrodinium impudicum by analyzing the ITS regions of the 5.8S rDNA, 18S rDNA, morphological phenotype and fatty acid composition. The exopolysaccharide production and cell growth in a 300-ml photobioreactor were increased 2.7- and 2.4-fold, respectively, compared with that in a flask culture at the first isolation step.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2006.03a
• /
• pp.286-300
• /
• 2006

Nonlinear cross-anisotropic properties of soil is critical for exact numerical simulation. Theoretically, initial cross-anisotropic properties can be evaluated from triaxial tests with bender element tests, and nonlinear cross-anisotropic properties over initial strain level cannot be evaluated from triaxial tests. In this study, a supposed condition among nonlinear cross-anisotropic properties is suggested to calculate nonlinear cross-anisotropic properties from triaxial tests. Maximum strain and incremental strain energy are applied to combine triaxial test results and theoretical normalized shear modulus curve, respectively Based on combined results, nonlinear cross-anisotropic properties are calculated. Numerical simulation for triaxial tests Is carried out to verify the applicability of the supposed condition with calculated cross-anisotropic properties and simplified nonlinear cross-anisotropic model.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.8
• /
• pp.1257-1264
• /
• 2015

Trichoderma strain T-33 has been demonstrated to have inhibitory effect on the fungus species Cytospora chrysosperma. Here, an active antifungal compound was obtained from Trichoderma strain T-33 extract via combined separation technologies, including organic solvent extraction, liquid chromatography, and thin-layer chromatography. The purified compound was further characterized by advanced analytical technologies to elucidate its chemical structure. Results indicated that the active antifungal compound in Trichoderma strain T-33 extract is 2,5-cyclohexadiene-1,4-dione-2,6-bis (1,1-dimethylethyl).

• Advances in materials Research
• /
• v.12 no.2
• /
• pp.133-159
• /
• 2023

In this paper, a size-dependent dynamic investigation of a porous metal foams microbeamsis presented. The novelty of this study is to use a metal foam microbeam that contain porosities based on the refined high order shear deformation beam model, with sinusoidal shear strain function, and the modified strain gradient theory (MSGT) for the first time. The Lagrange's principle combined with differential quadrature hierarchicalfinite element method (DQHFEM) are used to obtain the porous microbeam governing equations. The solutions are presented for the natural frequencies of the porous and homogeneoustype microbeam. The obtained results are validated with the analytical methods found in the literature, in order to confirm the accuracy of the presented resolution method. The influences of the shape of porosity distribution, slenderness ratio, microbeam thickness, and porosity coefficient on the free vibration of the porous microbeams are explored in detail. The results of this paper can be used in various design formetallic foammicro-structuresin engineering.

• Structural Engineering and Mechanics
• /
• v.88 no.4
• /
• pp.355-368
• /
• 2023

Employing the non-local strain gradient theory (NSGT), this paper investigates the nonlinear resonance characteristics of functionally graded material (FGM) nanoshells with initial geometric imperfection for the first time. The effective material properties of the porous FGM nanoshells with even distribution of porosities are estimated by a modified power-law model. With the guidance of Love's thin shell theory and considering initial geometric imperfection, the strain equations of the shells are obtained. In order to characterize the small-scale effect of the nanoshells, the nonlocal parameter and strain gradient parameter are introduced. Subsequently, the Euler-Lagrange principle was used to derive the motion equations. Considering three boundary conditions, the Galerkin principle combined with the modified Lindstedt Poincare (MLP) method are employed to discretize and solve the motion equations. Finally, the effects of initial geometric imperfection, functional gradient index, strain gradient parameters, non-local parameters and porosity volume fraction on the nonlinear resonance of the porous FGM nanoshells are examined.

• Journal of Korean Foot and Ankle Society
• /
• v.1 no.1
• /
• pp.38-42
• /
• 1997

It was well known that arthrodesis of the tarsal joint is an exellent procedure to correct the flatfoot deformity for relieving pain. Recently, concept of the selective tarsal joint fusion instead of the triple fusion was developedto preserve the joint motion. To investigate and compare the effect of the each different tarsal fusion, we measured the strain at the spring ligament, medial roof of the medial longitudianl arch. Five fresh frozen cadevar foot specimens, with distal half of the tibia were utilized. The superomedial portion of the spring ligament complex was dissected from the origin to the insertion. For each specimen, a calibrated open liquid metal strain guage was secured along the length of the superomedial portion. Under the specially devised test rig, measurement of the strain was taken at each test condition from the tare weight 18.2 lb followed by 38.2 lb., 82.2 lb and a maximum loads of 134.6 lb. : 1) unfused condition, 2) isolated subtalar fusion 3) isolated talonavicular fusion 4) combined subtalar and talonavicular fusion 5) triple fusion. Statistics showed that siginificant reduction in strain following the triple fusion, and from the subtalar fusion to triple fusion.

• Structural Engineering and Mechanics
• /
• v.90 no.1
• /
• pp.1-18
• /
• 2024

The present study conducts a thorough analysis of thermal vibrations in functionally graded porous nanocomposite beams within a thermal setting. Investigating the temperature-dependent material properties of these beams, which continuously vary across their thickness in accordance with a power-law function, a finite element approach is developed. This approach utilizes a nonlocal strain gradient theory and accounts for a linear temperature rise. The analysis employs four different patterns of porosity distribution to characterize the functionally graded porous materials. A novel two-variable shear deformation beam nonlocal strain gradient theory, based on trigonometric functions, is introduced to examine the combined effects of nonlocal stress and strain gradient on these beams. The derived governing equations are solved through a 3-nodes beam element. A comprehensive parametric study delves into the influence of structural parameters, such as thicknessratio, beam length, nonlocal scale parameter, and strain gradient parameter. Furthermore, the study explores the impact of thermal effects, porosity distribution forms, and material distribution profiles on the free vibration of temperature-dependent FG nanobeams. The results reveal the substantial influence of these effects on the vibration behavior of functionally graded nanobeams under thermal conditions. This research presents a finite element approach to examine the thermo-mechanical behavior of nonlocal temperature-dependent FG nanobeams, filling the gap where analytical results are unavailable.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.33 no.9
• /
• pp.949-956
• /
• 2009

The C(t)-integral describes amplitude of stress and strain rate field near a tip of stationary crack under transient creep condition. Thus the C(t)-integral is a key parameter for the high-temperature crack assessment. Estimation formulae for C(t)-integral of the cracked component operating under mechanical load alone have been provided for decades. However, high temperature structures usually work under combined mechanical and thermal load. And no investigation has provided quantitative estimates for the C(t)-integral under combined mechanical and thermal load. In this study, 3-dimensional finite element analyses were conducted to calculate the C(t)-integral of elastic-creep material under combined mechanical and thermal load. As a result, redistribution time for the crack under combined mechanical and thermal load is re-defined through FE analyses to quantify the C(t)-integral. Estimates of C(t)-integral using this proposed redistribution time agree well with FE analyses results.

• Steel and Composite Structures
• /
• v.42 no.5
• /
• pp.657-669
• /
• 2022

Structural materials can experience large plastic deformation under extreme cyclic loading that is caused by events like earthquakes. To evaluate the seismic safety of a structure, accurate numerical material models should be used. For a steel structure, the cyclic strain hardening behavior of structural steel should be correctly modeled. In this study, a combined hardening model, consisting of one isotropic hardening model and three nonlinear kinematic hardening models, was used. To determine the values of the combined hardening model parameters efficiently and accurately, the improved opposition-based particle swarm optimization (iOPSO) model was adopted. Low-cycle fatigue tests were conducted for three steel grades commonly used in Korea and their modeling parameters were determined using iOPSO, which was first developed in Korea. To avoid expensive and complex low cycle fatigue (LCF) tests for determining the combined hardening model parameter values for structural steel, empirical equations were proposed for each of the combined hardening model parameters based on the LCF test data of 21 steel grades collected from this study. In these equations, only the properties obtained from the monotonic tensile tests are required as input variables.

• Geomechanics and Engineering
• /
• v.15 no.5
• /
• pp.1113-1124
• /
• 2018

Geological dynamic hazards during deep coal mining are caused by the failure of a composite system consisting of the rock and coal layers, whereas the joint in coal affects the stability of the composite system. In this paper, the compression test simulations for the rock-coal combined body with single joint in coal were conducted using $PFC^{2D}$ software and especially the effects of joint length and joint angle on strength and failure characteristics in a rock-coal combined body were analyzed. The joint length and joint angle exhibit a deterioration effect on the strength and affect the failure modes. The deterioration effect of joint length of L on the strength can be neglected with a tiny variation at ${\alpha}$ of $0^{\circ}$ or $90^{\circ}$ between the loading direction and joint direction. While, the deterioration effect of L on strength are relatively large at ${\alpha}$ between $30^{\circ}$ and $60^{\circ}$. And the peak stress and peak strain decrease with the increase of L. Additionally, the deterioration effect of ${\alpha}$ on the strength becomes larger with the increase of L. With the increase of ${\alpha}$, the peak stress and peak strain first decrease and then increase, presenting "V-shaped" curves. And the peak stress and peak strain at ${\alpha}$ of $45^{\circ}$ are the smallest. Moreover, the failure mainly occurs within the coal and no apparent failure is observed for rock. At ${\alpha}$ between $30^{\circ}$ and $60^{\circ}$, the secondary shear cracks generated in or close to the joint tips, cause the structural instability failure of the combined body. Therefore, their failure models present as a shear failure along partial joint plane direction and partially cutting across the coal body or a shear failure along the joint plane direction. However, at ${\alpha}$ of $60^{\circ}$ and L of 10 mm, the "V-shaped" shear cracks cutting across the coal body cause its final failure. While crack nucleations at ${\alpha}$ of $0^{\circ}$ or $90^{\circ}$ are randomly distributed in the coal, the failure mode shows a V-shaped shear failure cutting across the coal body.