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Axenic Culture of Gyrodinium impudicum Strain KG03, a Marine Red-tide Microalga that Produces Exopolysaccharide  

Yim Joung Han (Korea Polar Research Institute, KORDI)
Lee Hong Kum (Korea Polar Research Institute, KORDI)
Publication Information
Journal of Microbiology / v.42, no.4, 2004 , pp. 305-314 More about this Journal
Abstract
An exopolysaccharide-producing microalgal dinoflagellate was isolated from a red-tide bloom and des­ignated strain KG03. A bacteria-free culture of strain KG03 was achieved using a modified wash with phototaxis and antibiotic treatment. Combined treatment with neomycin and cephalosporin was the most effective for eliminating the bacteria associated with the microalgae. Strain KG03 was identified as Gyrodinium impudicum by analyzing the ITS regions of the 5.8S rDNA, 18S rDNA, morphological phenotype and fatty acid composition. The exopolysaccharide production and cell growth in a 300-ml photobioreactor were increased 2.7- and 2.4-fold, respectively, compared with that in a flask culture at the first isolation step.
Keywords
Gyrodinium impudicum; axenic culture; exopolysaccharide; cell growth;
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