• Title/Summary/Keyword: Combined sequence

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Occurrence of Gray Mold Caused by Botrytis cinerea on Rubus crataegifolius in Korea (Botrytis cinerea에 의한 산딸기 잿빛곰팡이병의 발생)

  • Kim, Seung-Han;Park, Sangkyu;Lee, Seung-Yeol;Kwak, Youn-Sig;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.45 no.3
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    • pp.251-257
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    • 2017
  • The occurrence of gray mold on Rubus crataegifolius has recently been reported in Pohang, Gyeongbuk province, Korea. The initial symptom was the appearance of small brown spots on the leaves, and these lesions became later covered with a gray fungus as the disease progressed. A fungus was isolated from symptomatic leaves and incubated. Through morphological and phylogenetic analyses, the causal agent of the disease was identified to be Botrytis cinerea. The fungal isolate was then used to inoculate on the leaves of healthy plants to determine its pathogenicity as the causal agent of gray mold as per Koch's postulates. The inoculated leaves showed the same symptoms as the originally infected plant, and the fungal pathogen re-isolated from the lesions showed the same morphological characteristics as the original pathogen. This is the first report on the occurrence of gray mold on R. crataegifolius caused by B. cinerea in Korea.

Efficiency of RAPD and ISSR Markers in Differentiation of Homo- and Heterokaryotic Protoclones of Agaricus bisporus

  • Mahmudul, Islam Nazrul;Bian, Yin-Bing
    • Journal of Microbiology and Biotechnology
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    • v.20 no.4
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    • pp.683-692
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    • 2010
  • Morphologically, nine different slow-growing protoclones were screened from regenerated protoplasts of heterokaryotic Agaricus bisporus. As such, the present study is the first report on differentiating homo- and heterokaryotic protoclones using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Among 80 primers tested, the seven ISSR and seven RAPD primers selected for the analysis generated a total of 94 ISSR and 52 RAPD fragments, respectively. The ISSR fingerprinting also detected more polymorphic loci (38.29%) than the RAPD fingerprinting (34.61%). A principal coordinate analysis (PCA) was employed to evaluate the resolving power of the markers as regards differentiating protoclones. As a result, the mean polymorphism information content (PIC) for each marker system (i.e., 0.787 for RAPD and 0.916 for ISSR) suggested that ISSR is more effective for determining polymorphisms. The dendrograms constructed using RAPD, ISSR, and an integrated RAPD and ISSR marker system were highly correlated with one another as revealed by a high Mantel correlation (r= 0.98). The pairwise similarity index values also ranged from 0.64 to 0.95 (RAPD), 0.67 to 0.98 (ISSR), and 0.67 to 0.98 (RAPD and ISSR), whereas the mean similarity index values of 0.82, 0.81, and 0.84 were obtained for the RAPD, ISSR, and combined data, respectively. As there was a good correspondence between the RAPD and ISSR similarity matrices, ISSR would appear to be an effective alternative to RAPD in the genetic diversity assessment and accurate differentiation of homo- and heterokaryotic protoclones of A. bisporus.

Clinical Analysis of Varicose Veins in the Lower Extremities - an analysis of 227 operative cases - (하지정맥류의 임상양상 - 227 수술예의 분석 -)

  • 최수승
    • Journal of Chest Surgery
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    • v.32 no.11
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    • pp.1036-1041
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    • 1999
  • Background: To know the clinical aspects of varicose vein and the effectiveness of operative treatment, the patients who had been operated during the five and a half years were reviewed. Material and Method: From September 1993 to February 1999, 227 patients with varicose vein were operated upon. These patients were reviewed with history taking, physical examination, laboratory tests, operation and follow up visits at an out patient department. Result: The ages of the patients were from 20 to 69 years, mean 40.2 years. varicose veins. Occupations of the patients include housewives, store or factory owners, cooks, waiter or waitresses in a restaurant, salary men(women), teachers, hair stylists, professional athletes, in sequence, most of them require standing all day long. The 126 women had experienced child birth, among them 116 patients delivered more than 2 babies. All patients had protruding leg veins as the symptom. Most of them(61%) had pain. Other additional symptoms were heaviness, heatness, tingling, cramp, fatigue, etc. 68.3% of the patients had the symptoms for more than 10 years. Anatomical location of varicose vein was in long saphenous vein in 157 patients, in small saphenous vein in 27, and in other regions(combined or perforating vein) in 43 patients. There was no operative mortality. There were 2 patients who underwent reoperation due to recurrence during this study period. In one patient, it recurred in the distal perforating vein in small saphenous vein. The other patient had recurrence at the saphenofemoral junction in groin. The patients are doing well after the reoperation, but a long term observation is needed. Conclusion: Clinical aspects of varicose vein were reviewed. For the patients with varicose vein who had saphenofemoral regurgitation, operative treatment seemed to bea safe and effective modality of treatment.

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A Study on Video Data Protection Method based on MPEG using Dynamic Shuffling (동적 셔플링을 이용한 MPEG기반의 동영상 암호화 방법에 관한 연구)

  • Lee, Ji-Bum;Lee, Kyoung-Hak;Ko, Hyung-Hwa
    • Journal of Korea Multimedia Society
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    • v.10 no.1
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    • pp.58-65
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    • 2007
  • This dissertation proposes digital video protection algorithm lot moving image based on MPEG. Shuffling-based encryption algorithms using a fixed random shuffling table are quite simple and effective but vulnerable to the chosen plaintext attack. To overcome this problem, it is necessary to change the key used for generation of the shuffling table. However, this may pose a significant burden on the security key management system. A better approach is to generate the shuffling table based on the local feature of an image. In order to withstand the chosen plaintext attack, at first, we propose a interleaving algorithm that is adaptive to the local feature of an image. Secondly, using the multiple shuffling method which is combined interleaving with existing random shuffling method, we encrypted the DPCM processed 8*8 blocks. Experimental results showed that the proposed algorithm needs only 10% time of SEED encryption algorithm and moreover there is no overhead bit. In video sequence encryption, multiple random shuffling algorithms are used to encrypt the DC and AC coefficients of intra frame, and motion vector encryption and macroblock shuffling are used to encrypt the intra-coded macroblock in predicted frame.

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Association of Carotid Intraplaque Hemorrhage and Territorial Acute Infarction in Patients with Acute Neurological Symptoms Using Carotid Magnetization-Prepared Rapid Acquisition with Gradient-Echo

  • Park, Jung Soo;Kwak, Hyo Sung;Lee, Jong Myong;Koh, Eun Jeong;Chung, Gyung Ho;Hwang, Seung Bae
    • Journal of Korean Neurosurgical Society
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    • v.57 no.2
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    • pp.94-99
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    • 2015
  • Objective : The purpose of our study was to assess prevalence of carotid intraplaque hemorrhage (IPH) and associations between territorial acute infarction and IPH on magnetization-prepared rapid acquisition with gradient-echo (MPRAGE) in patients with acute neurologic symptoms. Methods : 83 patients with suspected acute neurologic symptoms were evaluated with both brain diffusion weighted imaging (DWI) and carotid MPRAGE sequences. Carotid plaque with high signal intensity on MPRAGE of >200% that of adjacent muscle was categorized as IPH. We analyzed the prevalence of IPH and its correlation with territorial acute infarction. Results : Of 166 arteries, 39 had a carotid artery plaque. Of these arteries, 26 had carotid artery stenosis less than 50%. In all carotid arteries, MR-depicted IPH was found in 7.2% (12/166). High-signal intensity on DWI was found in 17.5% (29/166). Combined lesion with ipsilateral high-signal intensity on DWI and IPH on carotid MPRAGE sequence was found in 6 lesions (6/166, 3.6%). Of patients with carotid artery plaque, MR-predicted IPH was found in 30.8% (12/39) and match lesions with high-signal intensity on DWI and MPRAGE was found in 15.4% (6/39). MR-predicted IPH was significantly higher prevalence in high-grade stenosis group (p=0.010). Relative risk between carotid MPRAGE-positive signal and ipsilateral high-signal intensity on DWI in arteries with carotid artery plaques was 6.8 (p=0.010). Conclusion : Carotid MPRAGE-positive signal in patients was associated with an increased risk of territorial acute infarction as detected objectively by brain DWI. The relative risk of stroke was increased in high-grade stenosis categories.

Difference of Physiochemical Characteristics Between Citrus Bacterial Canker Pathotypes and Identification of Korean Isolates with Repetitive Sequence PCRs

  • Lee, Yong-Hoon;Lee, Seung-Don;Lee, Dong-Hee;Yu, Sang-Mi;Lee, Jung-Hee;Heu, Sung-Gi;Hyun, Jae-Wook;Ra, Dong-Soo;Park, Eun-Woo
    • The Plant Pathology Journal
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    • v.24 no.4
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    • pp.423-432
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    • 2008
  • The difference of carbon source utilization and fatty acid composition between the pathotypes of Xanthomonas strains, which causing citrus bacterial canker was compared, and the physiochemical characteristics were used to analyze relationship of the strains for the first time. The pattern of several carbon sources utilization and fatty acids composition reliably discriminated the pathotypes of Xanthomonas strains. The dendrogram which was constructed by 95 carbon source utilization profiles differentiated X. axonopodis pv. citri A, $A^*$ and $A^w$ from the other pathotypes. When the dendrogram was drawn by combined analysis of carbon source utilization pattern and fatty acid composition, X. axonopodis pv. aurantifolii B, C and X. axonopodis pv. citrumelo formed a distinct cluster. The difference of carbon source utilization and fatty acid composition could be used effectively for the identification of pathotypes of citrus bacterial canker. The physiochemical characteristics strongly indicated that the strains isolated in Korea belong to X. axonopodis pv. citri A type. The cluster analysis by the band patterns of ERIC-, BOX- and REP-PCR allowed the discrimination of the pathotypes isolated from Korea. However, the rep-PCRs could not differentiate X. axonopodis pv. citri A types from $A^*$ and $A^w$ types. The overall results of metabolic profiles and rep-PCRs strongly indicated that the Korean isolates are X. axonopodis pv. citri A type.

Multiple Linkage Disequilibrium Mapping Methods to Validate Additive Quantitative Trait Loci in Korean Native Cattle (Hanwoo)

  • Li, Yi;Kim, Jong-Joo
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.7
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    • pp.926-935
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    • 2015
  • The efficiency of genome-wide association analysis (GWAS) depends on power of detection for quantitative trait loci (QTL) and precision for QTL mapping. In this study, three different strategies for GWAS were applied to detect QTL for carcass quality traits in the Korean cattle, Hanwoo; a linkage disequilibrium single locus regression method (LDRM), a combined linkage and linkage disequilibrium analysis (LDLA) and a $BayesC{\pi}$ approach. The phenotypes of 486 steers were collected for weaning weight (WWT), yearling weight (YWT), carcass weight (CWT), backfat thickness (BFT), longissimus dorsi muscle area, and marbling score (Marb). Also the genotype data for the steers and their sires were scored with the Illumina bovine 50K single nucleotide polymorphism (SNP) chips. For the two former GWAS methods, threshold values were set at false discovery rate <0.01 on a chromosome-wide level, while a cut-off threshold value was set in the latter model, such that the top five windows, each of which comprised 10 adjacent SNPs, were chosen with significant variation for the phenotype. Four major additive QTL from these three methods had high concordance found in 64.1 to 64.9Mb for Bos taurus autosome (BTA) 7 for WWT, 24.3 to 25.4Mb for BTA14 for CWT, 0.5 to 1.5Mb for BTA6 for BFT and 26.3 to 33.4Mb for BTA29 for BFT. Several candidate genes (i.e. glutamate receptor, ionotropic, ampa 1 [GRIA1], family with sequence similarity 110, member B [FAM110B], and thymocyte selection-associated high mobility group box [TOX]) may be identified close to these QTL. Our result suggests that the use of different linkage disequilibrium mapping approaches can provide more reliable chromosome regions to further pinpoint DNA makers or causative genes in these regions.

Additional mitochondrial DNA sequences from the dragonfly, Nannophya pygmaea (Odonata: Libellulidae), which is endangered in South Korea

  • Wang, Ah Rha;Kim, Min Jee;Kim, Sung Soo;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.35 no.1
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    • pp.51-57
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    • 2017
  • The tiny dragonfly, Nannophya pygmaea (Odonata: Libellulidae), is an endangered insect in South Korea. Previously, a partial mitochondrial DNA sequence that corresponded to a DNA barcoding region has been used to infer genetic diversity and gene flow. In this study, we additionally sequenced the barcoding region from N. pygmaea that had been collected from three previously sampled populations (40 individuals) and these sequences were combined with the preexisting data. We also selected and sequenced an additional mitochondrial gene (ND5) to find further variable gene regions in the mitochondrial genome. DNA barcoding sequences of 108 individuals from five South Korean localities showed that genetic diversity was highest in Gangjin, Jeollanam-do Province. Muuido, which was previously occupied by a single haplotype, was also found to have an identical haplotype, which confirmed the low genetic diversity on this islet. Gene flow among populations is highly limited, and no clear distance- or region-based geographic partitioning was observed. Phylogenetic relationships among haplotypes showed that there were no discernable haplotypes in South Korea. ND5 provided slightly more haplotypes compared to the barcoding region in 40 individuals (14 vs. 10 haplotypes in the COI gene). It also had a slightly higher within-locality diversity estimate, which suggested that ND5 had potential as mitochondrial DNA-based marker for population genetic analysis.

A Study on the Modularity in Clothing Design (의복디자인에서의 모듈성에 대한 연구)

  • Lim, So-Yon;Lee, Joo-Hyeon
    • Journal of the Korea Fashion and Costume Design Association
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    • v.14 no.2
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    • pp.1-10
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    • 2012
  • Clothing design is one of design products for human, the end user and corresponds to artifacts. Sometimes artifacts with one mono module exist, and so does artifacts assembled and combined with multi modules of same shape and size or various shapes and sizes and thus all design products can be understood by modularity concept. The purpose of this study was to make a new suggestion for the clothing design and production of high uniqueness and creativity by reviewing and synthesizing the foundation of clothing modularity concept as the original clothing design figures from the history have shown various modularity concept evolution from mono module to multi module stages. The methods of this study were to identify clothing modularity and analyze the type, evolving direction, and category of clothing modularity, and the value of clothing modularization design through comprehensive literature reviews on topic-related books and theses. The original clothing figures with Significance from the clothing history were analyzed in evolution sequence for application direction and value of clothing modularity in flat pattern. Clothing modularity in ancient clothing figures was classified as three types of the fixed, drapery, and straight lined in evolving direction from clothing of mono module to flat patterned clothing. The direction of clothing modularity was identified as mono- dual-triple-multi into another level of multi modularity after intentional devolution. The categorization of clothing modularity was identified in terms of clothing flat construction, clothing design construction, and clothing form modeling. The value of clothing modularization design using clothing modularity was identified as economical efficiency, convenience, promptitude, adaptability, functionality, and creativity.

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Interaction with Agents in the Virtual Space Combined by Recognition of Face Direction and Hand Gestures (얼굴 방향과 손 동작 인식을 통합한 가상 공간에 존재하는 Agent들과의 상호 작용)

  • Jo, Gang-Hyeon;Kim, Seong-Eun;Lee, In-Ho
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.39 no.3
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    • pp.62-78
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    • 2002
  • In this paper, we describe a system that can interact with agents in the virtual space incorporated in the system. This system is constructed by an analysis system for analyzing human gesture and an interact system for interacting with agents in the virtual space using analyzed information. An implemented analysis system for analyzing gesture extracts a head and hands region after taking image sequence of an operator's continuous behavior using CCD cameras. In interact system, we construct the virtual space that exist an avatar which incarnating operator himself, an autonomous object (like a Puppy), and non-autonomous objects which are table, door, window and object. Recognized gesture is transmitted to the avatar in the virtual space, then transit to next state based on state transition diagram. State transition diagram is represented in a graph in which each state represented as node and connect with link. In the virtual space, the agent link an avatar can open and close a window and a door, grab or move an object like a ball, order a puppy to do and respond to the Puppy's behavior as does the puppy.