• Title/Summary/Keyword: Combined sequence

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Occurrence of Leaf Blight on Cosmos Caused by Alternaria cosmosa in Korea

  • Deng, Jian Xin;Lee, Ji Hye;Paul, Narayan Chandra;Cho, Hye Sun;Lee, Hyang Burm;Yu, Seung Hun
    • The Plant Pathology Journal
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    • v.31 no.1
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    • pp.78-82
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    • 2015
  • In 2011, a leaf blight disease was observed on cosmos (Cosmos bipinnatus) leaves in Nonsan, Korea. The causal pathogen was isolated and identified based on morphological and molecular approaches. Morphological characteristics of the pathogen matched well with the Alternaria cosmosa and also easily distinguishable from Alternaria zinniae reported from cosmos seeds by producing branched beak. Phylogenetically, the pathogen could not be distinguished from A. passiflorae based on the sequence analysis of a combined data set of Alt a1 and gpd genes. However, A. passiflorae was distinguished from the present species by having conidiophores with 4 to 5 conidiogenous loci. The results indicate that the present Alternaria species is A. cosmosa. Pathogenicity tests revealed that the isolate was pathogenic to the leaves of Cosmos bipinnatus. This is the first report of Alternaria blight disease caused by A. cosmosa on cosmos in Korea.

New Record of Two Derbesia Species (Chlorophyta) in Korea

  • An, Jae Woo;Kang, Pil Joon;Nam, Ki Wan
    • Korean Journal of Environmental Biology
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    • v.36 no.2
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    • pp.150-155
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    • 2018
  • Two siphonous green algae were collected from the eastern coast of Korea. These species share the typical features of Derbesia sporophytes, such as erect and prostrate siphonous filaments and the presence of basal septum in lateral branches. One is characterized by the combined features of a relatively small tufted sporophytic thalli arising from a rhizoidal base, subdichotomously branched filaments with a basal septum and one pyrenoid per subspherical to lenticular chloroplast. The other shows a larger sporophytic thallus, sparsely subdichotomously branched filaments with a basal septum and one pyrenoid per spherical chloroplast. In a phylogenetic tree based on rbcL sequence, the two above-mentioned Korean algae nest in the same clades as Derbesia minima and D. indica, respectively. The genetic distance between the sequences within the clades was 0.5-0.8%, which is considered to be included in the intra-specific range for the genus. These two siphonous Korean algae are identified as D. minima and D. indica, respectively, based on the morphological and molecular analyses. These species are newly recorded in the Korean marine algal flora herein.

Study of 3 dimensional wide area continuous laser micro patterning (3차원 대면적 연속 마이크로 레이저 패터닝을 위한 연구)

  • Kim, Kyunghan;Sohn, Hyonkee;Lee, Jaehoon
    • Laser Solutions
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    • v.18 no.4
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    • pp.1-5
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    • 2015
  • For continuous laser micro patterning on three-dimensional free form surface, innovative laser system is developed. The two axis galvanometer is combined with the dynamic focusing unit to increase optical distance. Also, it is synchronized with the 3 axis mechanical system. To determine laser machining sequence, laser CAM system is developed. It can make possible of 3D surface micro patterning under $25{\mu}m$ pattern width. The uniformity of pattern width is about 2.8% and it is validated that focal plane is well conserved by the dynamic focusing unit. Velocity and positional information of 1 axis is stage is fed to the scanner control board by the encoder signal and it makes possible real time synchronization. With this system, possible patterning volume is enlarged from $40{\times}40mm^2$ to $40{\times}120{\times}30mm^3$.

Characteristic Graph를 利用한 組合論理回路의 故障診斷

  • 林寅七 = In-Chil Lim;李亮熙
    • Communications of the Korean Institute of Information Scientists and Engineers
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    • v.5 no.1
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    • pp.42-49
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    • 1987
  • This paper describes test-pattern generation and it;s sequence for fan out-free Combinational logic network with multiple faults. The method for detecting multiple faults, in systematic way, is established by using characteristic graphs. This method is applied even in the case of fan out-reconvergent combinational logic networks. In this case, the network is decomposed into a set of fan out-free sybnetworks so as to use the characteristic graphs, and minimal test patterns are generated seperately. The each test set is combined and the test pattern for fan out-reconvergent networks are derived. According to corresponding characteristic graph, additional test patterns to detect multiple faults are simply derived.

Screening of Fungal Strains Producing Lovastatin, an Antihypercholesterolemic Agent (콜레스테롤합성저해제 lovastatin을 생산하는 곰팡이 균주의 탐색)

  • Bang, In-Young;Whang, Seung-Whan;Kim, Jung-Wan;Kim, Sang-Yong;Park, Cheon-Seok
    • Korean Journal of Food Science and Technology
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    • v.35 no.3
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    • pp.442-446
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    • 2003
  • Over two hundred fungal strains from Korean soil were tested for the production of cholesterol-lowering agent, lovastatin. Each fungal strain was cultivated in the rapeseedmeal production medium (RPM). After growing for 7 days, the presence of lovastatin in the culture was examined by TLC analysis and HPLC. Nine different fungal strains were determined to produce detectable amounts of lovastatin, among which one fungal strain isolated from barnyard manure of Kanghwa island produced 25.58 mg/L of lovastatin in the production medium. The morphological observation combined with the determination of 18S-rRNA sequence suggested that the selected strain belongs to a member of Aspergillus sp..

Genetic Diversity in Cultured and Wild Populations of the Ascidian Halocynthia roretzi Inferred from Mitochondrial DNA Analysis

  • Yoon, Moon-Geun;Lee, Joo-Kyung;Jin, Hyung-Joo;Jin, Deuk-Hee
    • Fisheries and Aquatic Sciences
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    • v.12 no.1
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    • pp.44-48
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    • 2009
  • Nucleotide sequences of about 500 bp from the 5' end of mitochondrial (mt) DNA Cytochrome Oxidase I (COI) were analyzed to estimate the genetic variation between wild and cultured populations of the ascidian Halocynthia roretzi from two sites along the coast of Korea. A total of 25 haplotypes were defined by 21 variable nucleotide sites in the examined COI region. Genetic diversity (haplotype diversity and nucleotide divergence) of wild populations was higher than that of the cultured population. These data suggest that reduced genetic variation in the cultured population may have results from bottleneck effect caused by the use of a limited number of parental stock and pooling of gametes for fertilization. Pairwise population $F_{ST}$ estimates inferred that wild and cultured populations were genetically distinct. The combined results suggest that sequence polymorphism in the COI region would be preferable for estimating the genetic diversity of ascidian populations.

Azisymmetric Nonlinear Consolidation Analysis for Drainage-Installed Compressible Deposits (배수재가 설치된 압축성 지반의 축대칭 비선형 압밀해석)

  • 김윤태;이승래
    • Geotechnical Engineering
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    • v.12 no.1
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    • pp.5-20
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    • 1996
  • In order to accelerate the rate of consolidation settlement and to gain a required shear strength for a given soft clay deposit, the preloading technique combined with a vertical drainage system has been widely applied. In this'study, the theory of axisymmetric consolidation which considers the variation of compressibility and permeability during the conslidation process, has ben developed. A computer program named AXICON for the analysis of axisymmetric nonlinear consolidation is developed by adopting an explicit finite difference method. Smear and well resistance effects are also considered. The AXICON is capable of analyzing the consolidation behavior of multi -layered deposits and simulates time dependent loading sequence. The results of AXICON are validated with analytical solutions of Hansbo and Barron, and compared with insitu settlements and pore pressures measured in a soft clay deposit.

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An Efficient Fading Estimation and Compensation Techniques for Transmission of Trellis Coded 16 QAM in Wireless Communication Channel (무선통신채널에서 트렐리스 부호화한 16 QAM 신호전송을 위한 효율적인 페이딩 추정.보상방안)

  • 김순영;김정수;이광재;이문호
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.10 no.6
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    • pp.855-865
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    • 1999
  • This paper presents the improvement of BER performance using fading compensation method for 16 QAM-TCM to reduce the effects of multi-path fading in mobile radio environments. We propose the multi-pilot symbol aided fading compensation technique using Gausian interpolation method for inter-symbol interference or fading distortion occured in frequency selective fading channel. The proposed system is combined coding and modulation scheme for improving the reliability of a digital transmission system without increasing the transmitted power or the required bandwidth. In the fading compensation, the pilot symbols from a known sequence is multiplexed into the data symbols at regular intervals to from a frames for transmission. And we use a modified bit reversal block interleaver to randomize burst errors. The results show that significant improvements in the bit-error rate performances can be achieved by the proposed techniques.

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A New Performance Function-Based Control Strategy for Hybrid Series Active Power Filter in Three-Phase Four-Wire Systems (3상 4선식 하이브리드 형 직렬 능동전력필터에 대한 새로운 성능함수 제어 이론)

  • 신재화;김진선;김영석
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.52 no.11
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    • pp.563-571
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    • 2003
  • In this paper, the control algorithm and control method for a combined system of shunt passive filter and series active filter in 3-phase 4-wire system are discussed. Moreover, the three-phase four-wire system is widely employed in distributing electric energy to several office building and manufacturing plants. In such systems, the third harmonic and its 3th harmonics are termed as triple and zero sequence components that do not cancel each other in the system neutral. Consequently, the triple harmonics add together creating a primary source of excessive neutral current. Regarding this concern, this paper presents a new control algorithm for a series hybrid active system, whereas the control approach it adopts may directly influence its compensation characteristics. Hence, the advantage of this control algorithm is direct extraction of compensation voltage reference and the required rating of the series active filter is much smaller than that of a conventional shunt active filter. Some experiments were executed and experimental results from a prototype active power filter confirm the suitability of the proposed approach.

Improved Prediction of Coreceptor Usage and Phenotype of HIV-1 Based on Combined Features of V3 Loop Sequence Using Random Forest

  • Xu, Shungao;Huang, Xinxiang;Xu, Huaxi;Zhang, Chiyu
    • Journal of Microbiology
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    • v.45 no.5
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    • pp.441-446
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    • 2007
  • HIV-1 coreceptor usage and phenotype mainly determined by V3 loop are associated with the disease progression of AIDS. Predicting HIV-1 coreceptor usage and phenotype facilitates the monitoring of R5-to-X4 switch and treatment decision-making. In this study, we employed random forest to predict HIV-1 biological phenotype, based on 37 random features of V3 loop. In comparison with PSSM method, our RF predictor obtained higher prediction accuracy (95.1% for coreceptor usage and 92.1% for phenotype), especially for non-B non-C HIV-l subtypes (96.6% for coreceptor usage and 95.3% for phenotype). The net charge, polarity of V3 loop and five V3 sites are seven most important features for predicting HIV-1 coreceptor usage or phenotype. Among these features, V3 polarity and four V3 sites (22, 12, 18 and 13) are first reported to have high contribution to HIV-1 biological phenotype prediction.