• Korean Journal of Medicinal Crop Science
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• v.25 no.6
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• pp.375-380
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• 2017

Background: The objective of this study were to determine the antibacterial activity and antibiotic activity-enhancing effect of 70% ethanol extract of the root of Rumex japonicus Houtt. and its fractions when used in combination with gentamicin against aerobic skin flora. Methods and Results: The antibacterial activity and antibiotic (gentamicin) activity enhancing effect against aerobic skin flora were determined using the disc diffusion assay. Chloroform fraction (CF) and ethyl acetate fraction (EF) showed higher activities against Staphylococcus aureus and Staphylococcus epidermidis than those shown by other fractions. Regarding the antibiotic (gentamicin) activity-enhancing effect against aerobic skin flora, the n-hexane fraction (HF) and CF showed strong activity. The combination of HF and CF with gentamicin was evaluated using the broth dilution assay to determine the inhibitory effect on the growth of aerobic skin flora. The combination of CF with gentamicin exhibited the highest inhibitory effect on the growth of S. aureus and S. epdermidis. MTT assay performed to determine the viability of L929 cells revealed that EF treatment resulted in viability of 33.96 - 116.76% at the tested concentration. The combination of 70% ethanol extract and its other fractions with gentamicin showed low cell toxicity. Conclusions: Appropriate use of antimicrobial agents is important prior to the development of new antibiotics. The 70% ethanol extract of the root of R. japonicus Houtt. and its fractions showed significant synergism with gentamicin when used in combination against S. aureus and S. epdermidis. Thus, R. japonicus Houtt. could be used as a functional materials in antimicrobial-related fields.

• Biomolecules & Therapeutics
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• v.20 no.1
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• pp.62-67
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• 2012

TNF-related apoptosis-inducing ligand (TRAIL) is a promising agent for management of cancer because of its selective cytotoxicity to cancer cells. However, some cancer cells have resistance to TRAIL. Accordingly, novel treatment strategies are required to overcome TRAIL resistance. Here, we examined the synergistic apoptotic effect of apigenin in combination with TRAIL in Huh-7 cells. We found that combined treatment of TRAIL and apigenin markedly inhibited Huh-7 cell growth compared to either agent alone by inducing apoptosis. Combined treatment with apigenin and TRAIL induced chromatin condensation and the cleavage of poly (ADP-ribose) polymerase (PARP). In addition, enhanced apoptosis by TRAIL/apigenin combination was quantified by annexin V/PI flow cytometry analysis. Western blot analysis suggested that apigenin sensitizes cells to TRAIL-induced apoptosis by activating both intrinsic and extrinsic apoptotic pathway-related caspases. The augmented apoptotic effect by TRAIL/apigenin combination was accompanied by triggering mitochondria-dependent signaling pathway, as indicated by Bax/Bcl-2 ratio up-regulation. Our results demonstrate that combination of TRAIL and apigenin facilitates apoptosis in Huh-7 cells.

• Journal of Pharmacopuncture
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• v.25 no.2
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• pp.101-105
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• 2022

Objectives: This study aims to determine the Fractional Inhibitory Concentration Index (FICI) of combinations of Melastoma malabathricum leaf fraction with ciprofloxacin or gentamicin against pathogenic bacteria, Escherichia coli, Staphylococcus aureus, and Bacillus cereus, isolated from Diabetic Foot Ulcer (DFU) patients. Methods: We tested concentrations of 45%, 55%, 65%, and 75% of gentamicin and ciprofloxacin using dilution and agar diffusion methods. The combination of M. malabathricum leaf extract with these antibiotics was tested in vitro against all three bacteria. Results: The combination of M. malabathricum leaf extract and ciprofloxacin gave a FICI value of 0.5, indicating synergistic antibacterial activity against the test bacteria. Conclusion: The results show that the antibacterial effect of a combination of high doses of the leaf extract with either antibiotic is greater than that of the leaf extract and the antibiotics in single use.

• Journal of Environmental Science International
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• v.23 no.3
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• pp.369-378
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• 2014

Effect of improvement of the dielectric barrier discharge (DBD) plasma system on the inactivation performance of bacteria were investigated. The improvement of plasma reactor was performed by combination with the basic plasma reactor and UV process or combination with the basic plasma reactor and circulation system which was equipped with gas-liquid mixer. Experimental results showed that tailing effect was appeared after the exponential decrease in basic plasma reactor. There was no enhancement effect on the Ralstonia Solanacearum inactivation with combination of basic plasma process and UV process. The application of gas-liquid mixing device on the basic plasma reactor reduced inactivation time and led to complete sterilization. The effect existence of gas-liquid mixing device, voltage, air flow rate (1 ~ 5 L/min), water circulation rate (2.8 ~ 9.4 L/min) in gas-liquid mixing plasma, plasma voltage and UV power of gas-liquid mixing plasma+UV process were evaluated. The optimum air flow rate, water circulation rate, voltage of gas-liquid mixing system were 3 L/min, 3.5 L/min and 60 V, respectively. There was no enhancement effect on the Ralstonia Solanacearum inactivation with combination of gas-liquid mixing plasma and UV process.

• Journal of Environmental Science International
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• v.20 no.7
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• pp.891-898
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• 2011

The aim of this research was to evaluate the effect of combination of disinfection process (electrolysis, UV process) on Escherichia coli (E. coli) disinfection and oxidants (OH radical, $ClO_2$, HOCl, $H_2O_2$ and $O_3$) generation. The effect of electrolyte type (NaCl, KCl and $Na_2SO_4$) on the E. coli disinfection and oxidants generation were evaluated. The experimental results showed that performance of E. coli disinfection of electrolysis and UV single process was similar. Combination of electrolysis and UV process enhanced the E. coli disinfection and 4-carboxybenzaldehyde (4-CBA, indicator of the generation of OH radical) degradation. It is clearly showed synergy effect on disinfection and OH radical formation. However chlorine ($ClO_2$, HOCl) and oxygen type ($H_2O_2$, $O_3$) oxidants were decreased with the combination of two process. In electrolysis + UV complex process, electro-generated $H_2O_2$ and $O_3$ were reacted with UV light of UV-C lamp and increased 4-CBA degradation(increase OH radical). Disinfection of electrolyte of chlorine type was higher than that of the sulfate type electrolyte due to the higher generation of OH radical and oxidants.

• Journal of the Korean Home Economics Association
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• v.44 no.5 s.219
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• pp.69-78
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• 2006

The purpose of this study is to investigate the effect of eyeshadow color (brown, purple, and blue), lipstick color (red, red purple, and yellow red), and lipstick tone (vivid, light, dull, and dark) on impression formation. Sets of stimulus and response scales (7 point semantic) were used as experimental materials. The stimuli were 36 color pictures manipulated with the combination of eyeshadow color, lipstick color, and lipstick tone using computer simulation. The subjects were 216 female undergraduates living in Jinju city. Impression factor of the stimulus was composed of 4 different components, attractiveness and gracefulness, visibility, cuteness, and softness, of which the first two were important. Eyeshadow color, lipstick color, and lipstick tone independently influenced the attractiveness and gracefulness and visibility. In the cuteness and softness, eyeshadow color and lipstick tone showed independent effect. In the interaction effect, the combination of lipstick color and lipstick tone showed significant effect on the attractiveness and gracefulness, visibility, and cuteness. Finally, it was found that the combination of eveshadow color and tpstick tone showed significant difference in the softness.

• Steel and Composite Structures
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• v.4 no.3
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• pp.189-209
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• 2004

The commonly used seismic design procedures to evaluate the maximum effect of both horizontal components of earthquakes, namely, the Square Root of the Sum of the Squares (SRSS) and the 30-percent (30%) combination rules, are re-evaluated. The maximum seismic responses of four three-dimensional moment resisting steel frames, in terms of the total base shear and the axial loads at interior, lateral and corner columns, are estimated as realistically as possible by simultaneously applying both horizontal components. Then, the abovementioned combination rules and others are evaluated. The numerical study indicates that both, the SRSS rule and the 30% combination method, may underestimate the combined effect. It is observed that the underestimation is more for the SRSS than for the 30% rule. In addition, the underestimation is more for inelastic analysis than for elastic analysis. The underestimation cannot be correlated with the height of the frames or the predominant period of the earthquakes. A basic probabilistic study is performed in order to estimate the accuracy of the 30% rule in the evaluation of the combined effect. Based on the results obtained in this study, it is concluded that the design requirements for the combined effect of the horizontal components, as outlined in some code-specified seismic design procedures, need to be modified. New combination ways are suggested.

• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.858-864
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• 2007

This study investigated the antibacterial activities of sophoraflavanone G from Sophora flavescens in combination with two antimicrobial agents against oral bacteria. The combined effect of sophoraflavanone G and the antimicrobial agents was evaluated using the checkerboard method to obtain a fractional inhibitory concentration(FIC) index. The sophoraflavanone G+ampicillin(AM) combination was found to have a synergistic effect against S. mutans, S. sanguinis, S. sobrinus, S. gordonii, A. actinomycetemcomitans, F nucleatum, P. intermedia, and P. gingivalis, whereas the sophoraflavanone G+gentamicin(GM) combination had a synergistic effect against S. sanguinis, S. criceti, S. anginosus, A. actinomycetemcomitans, F nucleatum, P. intermedia, and P. gingivalis. Neither combination exhibited any antagonistic interactions(FIC index>4). In particular, the MICs/MBCs for all the bacteria were reduced to one-half$\sim$one-sixteenth as a result of the drug combinations. A synergistic interaction was also confirmed by time-kill studies for nine bacteria where the checkerboard suggested synergy. Thus, a strong bactericidal effect was exerted through the drug combinations, plus in vitro data suggested that sophoraflavanone G combined with other antibiotics may be microbiologically beneficial rather than antagonistic.

• Korean Institute of Interior Design Journal
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• no.34
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• pp.114-123
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• 2002

Koreans have regarded the red as a major color from old times. The red is a traditional color to have symbols of high position, national foundation and especially royalty. So, we can see the reds in the palace for kings very much.The purpose of this study is to draw out the features of color red in the Kyungbok palace. The color was analyzed by two categories-architectural buildings${\cdot}$structures and ornamental painted patterns. The major findings from this research are summarized as follows:1. Seokganju(similar to terra rossa) and toyugsaek(light seokganju) are found main colors in architectural space, while seokganju has a linear effect and toyuk has a facial effect. 2. Yugsaek(similar to light vermillion) and Jangdan(similar to orange) are found main colors in ornamental painted patterns. These colors are more vivid and brighter than those for architectural space.3. As for two-color combination, reds and blues(including greens) are found major combination both of architectural space and ornamental patterns. And reds and white are the following combination, which gives an bright image by white. 4. As for three-color combination, red-white-black combination of pediment and red-blue-white combination of openings are found very popular in architectural space, while red-blue-yellow combination is most popular in ornamental patterns.The reds are found dominant color of both architectural space and ornamental patterns in the Kyungbok palace. The color design as shown in the Kyungbok palace can be considered as the feature of traditional color design.

• Journal of the Korean Society of Clothing and Textiles
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• v.29 no.9_10 s.146
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• pp.1207-1218
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• 2005

The purpose of this study is to find out the effect of wearer's body type and clothing type, neutral tone combination on impression formation. The experimental materials developed for this study are a set of stimuli and response scales(The 7-point semantic). the stimuli are 90 pictures manipulated with wearer's body type, clothing type and neutral tone variation by computer simulation. The subjects are 540 female undergraduate students in Kyung-nam. The resulte of this study are as follows : Impression factor of the stimulus consisted of the 5 demensions(tenderness, cuteness, attractiveness, elegance, intrepidity). Wearer's body type had significant effects on all five factors. Especially, it had the strongest effects on attractiveness. Clothing type had significant effects on tenderness, attractiveness, intrepidity. Neutral tone combinations had significant effects on all factors except elegance. In regarding the effect of interaction between each variable, the combination of clothing type and neutral tone combination had significant effects on attractiveness, elegance, intrepidity.