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Antibacterial Activity of Sophoraflavanone G Isolated from the Roots of Sophora flavescens  

Cha, Jeong-Dan (Institute of Oral Bioscience and Department of Oral Microbiology, School of Dentistry, Chonbuk National University)
Jeong, Mi-Ran (Research Center of Bioactive Materials, Chonbuk National University)
Jeong, Seung-Il (Jeonju Biomaterials Institute)
Lee, Kyung-Yeol (Institute of Oral Bioscience and Department of Oral Microbiology, School of Dentistry, Chonbuk National University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.5, 2007 , pp. 858-864 More about this Journal
Abstract
This study investigated the antibacterial activities of sophoraflavanone G from Sophora flavescens in combination with two antimicrobial agents against oral bacteria. The combined effect of sophoraflavanone G and the antimicrobial agents was evaluated using the checkerboard method to obtain a fractional inhibitory concentration(FIC) index. The sophoraflavanone G+ampicillin(AM) combination was found to have a synergistic effect against S. mutans, S. sanguinis, S. sobrinus, S. gordonii, A. actinomycetemcomitans, F nucleatum, P. intermedia, and P. gingivalis, whereas the sophoraflavanone G+gentamicin(GM) combination had a synergistic effect against S. sanguinis, S. criceti, S. anginosus, A. actinomycetemcomitans, F nucleatum, P. intermedia, and P. gingivalis. Neither combination exhibited any antagonistic interactions(FIC index>4). In particular, the MICs/MBCs for all the bacteria were reduced to one-half$\sim$one-sixteenth as a result of the drug combinations. A synergistic interaction was also confirmed by time-kill studies for nine bacteria where the checkerboard suggested synergy. Thus, a strong bactericidal effect was exerted through the drug combinations, plus in vitro data suggested that sophoraflavanone G combined with other antibiotics may be microbiologically beneficial rather than antagonistic.
Keywords
Sophora flavescens; sophoraflavanone G; antibacterial activity; checkerboard method; time-kill method; synergic effect;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 12  (Related Records In Web of Science)
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