• 한국식물병리학회지
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• 제10권2호
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• pp.105-111
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• 1994

Pathogenic fungal species associated with apple anthracnose were studied with diseased fruits collected over two major apple growing areas from 1986 to 1988. Colletotrichum acutatum Simmonds, c. gloeosporioides(Penz.) Sacc. and Glomerella cingulata(Stonem.) Spauld. & Schrenk were identified based on their mycological characteristics. C. acutatum could be easily differentiated from C. gloeosporioides by producing their fusiform conidia and developing apricot∼salmon colony colors, non-forming an ascigerous stages. Growth rate of C. acutatum were slow in plate culture. The three species of anthracnose fungi tested caused apple rot. Symptoms produced by C. gloeosporioides were larger than those of the others. C. gloeosporioides and G. cingulata produced rot lesions on apples that appeared became brown and later black, but C. acutatum produced rot lesions appeared yellowish brown and later reddish brown. G. cingulata has the major species responsible for apple anthracnose, epidemics in Korea.

• 한국균학회지
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• 제51권4호
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• pp.335-347
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• 2023

Sordariomycetes is the second largest class of Ascomycota, distributed throughout various habitat including terrestrial and aquatic environments and also existing as endophytes. We isolated endophytic fungal strains in Korea, identifying them based on their morphological characteristics and molecular analyses, using eight specific DNA regions for accurate genus identification. We identified five previously unrecorded endophytic fungal species in Korea: Chaetomium subaffine, Colletotrichum jiangxiense, Colletotrichum sydowii, Diaporthe vacuae and Neurospora tetraspora. In this study, we describe the morphological characteristics and present our phylogenetic analyses of these five fungal species.

• Mycobiology
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• 제46권2호
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• pp.92-100
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• 2018

The filamentous Ascomycota Colletotrichum gloeosporioides sensu lato is a fungus that has been reported worldwide as a causal agent of anthracnose disease in avocado and other crops. In Mexico, this species affects fruits from an early stage of development in the orchard until the post-harvest stage. Although fungicides are continuously applied to control Colletotrichum species, pericarp cankers and soft rot mesocarp in fruits are still frequently observed. Considering the lack of a precise description of the causative agent, the aim of the current study was to determine the pathogens involved in this symptomatology. Twenty-four isolates were consistently obtained from the pericarp of avocado fruits cv. "Hass" collected in the central avocado-producing area of Mexico. Morphological features such as colony growth, conidia size, and mycelial appressorium were assessed. Bayesian multilocus phylogenetic analyses were performed using amplified sequences of the internal transcribed spacer region of the nuclear ribosomal DNA; actin, chitin synthase, glyceraldehyde-3-phosphate dehydrogenase partial genes; and APn2-Mat1-2 intergenic spacer and mating type Mat1-2 partial gene from the nine selected isolates. In addition, fruits were inoculated with a conidial suspension and reproducible symptoms confirmed the presence of Colletotrichum fructicola in this area. This pathogenic species can now be added to those previously reported in the country, such as C. acutatum, C. boninense, C. godetiae, C. gloeosporioides, and C. karstii. Disease management programs to reduce the incidence of anthracnose should include C. fructicola to determine its response to fungicides that are routinely applied, considering that the appearance of new species is affecting the commercial quality of the fruits and shifting the original population structure.

• The Plant Pathology Journal
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• 제37권5호
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• pp.465-475
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• 2021

The aim of this study was to characterize potential fungal species affecting mangrove species in Mexico. The phytopathogens were identified based on morphological and molecular characteristics using internal transcribed spacer (ITS1/ITS4) primers then sequenced and compared with the other related sequences in GenBank (NCBI). Three fungal species were identified as Colletotrichum queenslandicum (Weir and Johnst, 2012) from black mangrove (Avicennia germinans); Colletotrichum ti (Weir and Johnst, 2012) from white mangrove (Laguncularia racemosa) and buttonwood mangrove (Conocarpus erectus); Fusarium equiseti (Corda) from red mangrove (Rhizophora mangle). In addition, C. ti and F. equiseti were identified from mango Mangifera indica L. sampled close by the mangrove area. This study provides first evidence of anthracnose on four mangrove species caused by Colletotrichum and Fusarium species in the "Términos" coastal lagoon in Campeche State southern Mexico. This is the first time that C. queenslandicum and C. ti are reported in Mexico. F. equiseti has not been reported affecting M. indica and R. mangle until the present work. Little is known regarding fungal diseases affecting mangroves in Mexico. These ecosystems are protected by Mexican laws and may be threatened by these pathogenic fungus. This is the first report of the effect of Trichoderma harzianum TRICHO-SIN as an effective biological control against of Colletotrichum and Fusarium species.

• Mycobiology
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• 제48권4호
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• pp.321-325
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• 2020

A Colletotrichum species was isolated from leaves of Cymbidium exhibiting symptoms of anthracnose. In this study, the isolates obtained were identified based on recent taxonomic approaches for the genus Colletotrichum. The identity of the causal pathogen was confirmed using morphological data and phylogenetic analysis of combined multi-gene dataset (internal transcribed spacer, glyceraldehyde 3-phosphate dehydrogenase, chitin synthase-1, actin, histone3, beta-tubulin, and calmodulin). Pathogenicity testing revealed that the isolates were pathogenic to Cymbidium. Based on these results, the fungal pathogen occurring on Cymbidium orchids was identified as Colletotrichum cymbidiicola, which is a newly recorded species in Korea.

• Mycobiology
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• 제37권4호
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• pp.310-312
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• 2009

A total of 82 isolates of Colletotrichum species were obtained from anthracnose symptoms of highbush blueberry trees grown in the Gochang area of Korea during a disease survey in 2008. Out of the isolates, 75 were identified as Colletotrichum gloeosporioides and the others as C. acutatum based on their morphological and cultural characteristics. Twenty six of C. gloeosporioides isolates produced their teleomorph Glomerella cingulata in PDA culture. Three isolates of each C. gloeosporioides and C. acutatum caused anthracnose symptoms on the leaves by artificial inoculation, which were similar to what was observed in the orchards. Previously in Korea, only C. gloeosporioides has been reported as causing anthracnose in blueberries. This is the first report that C. acutatum causes anthracnose in the highbush blueberry in Korea.

• The Plant Pathology Journal
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• 제35권2호
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• pp.100-111
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• 2019

Glomerella leaf spot (GLS) caused by Colletotrichum spp. is a destructive disease of apple restricted to a few regions worldwide. The distribution and evolution of GLS symptoms were observed for two years in Uruguay. The recurrent ascopore production on leaves and the widespread randomized distribution of symptoms throughout trees and orchard, suggest that ascospores play an important role in the disease dispersion. The ability of ascospores to produce typical GLS symptom was demonstrated by artificial inoculation. Colletotrichum strains causing GLS did not result in rot development, despite remaining alive in fruit lesions. Based on phylogenetic analysis of actin, ${\beta}$-tubulin and glyceraldehyde-3-phosphate dehydrogenase gene regions of 46 isolates, 25 from fruits and 21 from leaves, C. karstii was identified for the first time causing GLS in Uruguay and C. fructicola was found to be the most frequent (89%) and aggressive species. The higher aggressiveness of C. fructicola and its ability on to produce abundant fertile perithecia could help to explain the predominance of this species in the field.

• Mycobiology
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• 제39권3호
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• pp.194-199
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• 2011

Pepper anthracnose caused by Colletotrichum species is one of the most important limiting factors for pepper production in Korea, its management being strongly dependent on chemicals. The aim of this work was to evaluate the possibilities of using silver nanoparticles instead of commercial fungicides. In this study, we evaluated the effect of silver nanoparticles against pepper anthracnose under different culture conditions. Silver nanoparticles (WA-PR-WB13R) were applied at various concentrations to determine antifungal activities in vitro and in the field. The application of 100 ppm concentration of silver nanoparticles produced maximum inhibition of the growth of fungal hyphae as well as conidial germination in comparison to the control in vitro. In field trials, the inhibition of fungi was significantly high when silver nanoparticles were applied before disease outbreak on the plants. Scanning electron microscopy results indicated that the silver nanoparticles caused a detrimental effect on mycelial growth of Colletotrichum species.

• The Plant Pathology Journal
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• 제40권4호
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• pp.399-407
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• 2024

Dieback disease in mango trees has been observed in Indonesia, particularly in Java Island, with the causal agent remaining unidentified. One of the important pathogens that are responsible for causing mango dieback is Colletotrichum. Field surveys were conducted in various mango cultivating areas in Java Island, Indonesia to assess prevalence of Colletotrichum as dieback disease pathogen. Eleven Colletotrichum isolates were recovered from symptomatic dieback twigs and morphologically characterized. Genetic diversity fingerprint analysis was carried out using rep-PCR. Phylogenetic analysis identified isolates as belonging to Colletotrichum asianum and Colletotrichum cairnsense using partial sequences of four gene regions, including ITS, ACT, GAPDH, and TUB2. Pathogenicity tests on mango seedlings cv. Arumanis showed that all fungal isolates were responsible for causing dieback symptoms. Subsequently, symptomatic tissue was reisolated to fulfill Koch's Postulate. This study represented new funding for two species of Colletotrichum causing mango dieback in Indonesia.

• The Plant Pathology Journal
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• 제24권1호
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• pp.17-23
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• 2008

A total of 33 isolates of Colletotrichum species obtained from pepper, apple, and strawberry in 2001 and 2002 were identified based on mycological characteristics, responses to fungicides carbendazim and the mixture of carbendazim and diethofencarb, and nucleotide sequence analysis of internal transcribed spacer (ITS) regionMost of the Colletotrichum isolates from pepper could be identified as C. acutatum. The pepper isolates produced grey white mycelia that gradually changed to dark gray. The conidia were variable in size, and almost cylindrical in shape with at least one rounded end. They could grow on PDA amended with carbendazim or with the mixture of carbendazim and diethofencarb at $10{\mu}g/ml$, to which the isolates from apple and strawberry were very sensitive. A part of the ITS regions from the Colletotrichum isolates was amplified with the specific primers designed for C. acutatum (Ca1-1) or C. gloeosporioides (Cg1-3). A primer pair of Ca1-1 and a universal primer (ITS4) amplified a 496-bp DNA fragment from all of the pepper isolates examined and one apple isolate. Taken together, it is conclusive that the Colletotrichum isolates causing the typical lesion of anthracnose on pepper fruits are C. acutatum.