Browse > Article
http://dx.doi.org/10.5423/PPJ.2008.24.1.017

Characterization of Colletotrichum Isolates Causing Anthracnose of Pepper in Korea  

Kim, Joon-Tae (Sesil Corporation)
Park, Sook-Young (Department of Agricultural Biotechnology, Seoul National University)
Choi, Woo-Bong (Department of Biotechnology and Bioengineering/Biomaterial Control, Dong-Eui University)
Lee, Yong-Hwan (Department of Agricultural Biotechnology, Seoul National University)
Kim, Heung-Tae (Department of Plant Medicine, Chungbuk National University)
Publication Information
The Plant Pathology Journal / v.24, no.1, 2008 , pp. 17-23 More about this Journal
Abstract
A total of 33 isolates of Colletotrichum species obtained from pepper, apple, and strawberry in 2001 and 2002 were identified based on mycological characteristics, responses to fungicides carbendazim and the mixture of carbendazim and diethofencarb, and nucleotide sequence analysis of internal transcribed spacer (ITS) regionMost of the Colletotrichum isolates from pepper could be identified as C. acutatum. The pepper isolates produced grey white mycelia that gradually changed to dark gray. The conidia were variable in size, and almost cylindrical in shape with at least one rounded end. They could grow on PDA amended with carbendazim or with the mixture of carbendazim and diethofencarb at $10{\mu}g/ml$, to which the isolates from apple and strawberry were very sensitive. A part of the ITS regions from the Colletotrichum isolates was amplified with the specific primers designed for C. acutatum (Ca1-1) or C. gloeosporioides (Cg1-3). A primer pair of Ca1-1 and a universal primer (ITS4) amplified a 496-bp DNA fragment from all of the pepper isolates examined and one apple isolate. Taken together, it is conclusive that the Colletotrichum isolates causing the typical lesion of anthracnose on pepper fruits are C. acutatum.
Keywords
Colletotrichum acutatum; ITS region; pepper anthracnose; PCR with species-specific primer;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 14  (Related Records In Web of Science)
연도 인용수 순위
1 Marinez-Culebras, P. V., Barrio, E., Garcia, M. D. and Querol, A. 2000. Identification of Colletotrichum species responsible for anthracnose of strawberry based on the internal transcribed spacers of the ribosomal region. FEMS Microbiology Letters 189:97-101.   DOI
2 Berstein, B., Zehr, E. I., Dean, R. A. and Shabi, E. 1995. Characteristics of Colletotrichum from peach, apple, pecan, and other hosts. Plant Dis. 79:478-482.   DOI   ScienceOn
3 Kim, Y. S., Min, J. Y., Kang, B. K., Bach, N. V., Choi, W. B., Park, E. W. and Kim, H. T. 2007. Analyses of the less benzimidazole- sensitivity of the isolates of Colletotrichum spp. causing the anthracnose in pepper and strawberry. Plant Pathol. J. 23:187-192.   과학기술학회마을   DOI   ScienceOn
4 Talhinhas, P., Sreenivasaprasad, S., Neves-Martins, J. and Oliveira, H. 2002. Genetic and morphological characterization of Colletotrichum acutatum causing anthracnose of lupins. Phytopathology 92:986-996.   DOI   ScienceOn
5 Urena-Padilla, A. R., MacKenzie, S. J., Bowen, B. W. and Legard, D. E. 2002. Etiology and population genetics of Colletotrichum spp. causing crown and fruit rot of strawberry. Phytopathology 92:1245-1252.   DOI   ScienceOn
6 Freeman, S. and Katan, T. 1997. Identification of Colletotrichum species responsible for anthracnose and root necrosis of strawberry in Israel. Phytopathology 87:516-521.   DOI   ScienceOn
7 Freeman, S., Katan, T. and Shabi, E. 1998. Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits. Plant Dis. 82:596-605.   DOI   ScienceOn
8 Gunnel, P. S. and Gubler, W. D. 1992. Taxonomy and morphology of Colletotrichum species pathogenic to strawberry. Mycologia 84:157-165.   DOI
9 Ishii, H., Iwamoto, S., Nishimura, K. and Fukaya, M. 1998. Comparative studies on fungicide sensitivity and other characteristics in Colletotrichum isolated from various plant species. pp. 529-534. Brighton crop protection conference -Pests & Diseases Vol. 1.
10 Adaskaveg, J. E. and Hartin, R. J. 1997. Characterization of Colletotrichum acutatum isolates causing anthracnose of almond and peach in California. Phytopathology 87:979-987.   DOI   ScienceOn
11 Afanador-Kafuri, L., Minz, D. Maymon, M. and Freeman, S. 2003. Characterization of Colletotrichum isolates from tamarillo, passiflora, and mango in Columbia and identification of a unique species from the genus. Phytopathology 93:579-587.   DOI   ScienceOn
12 Agostini, J. P., Timmer, L. W. and Mitchell, D. J. 1992. Morphological and pathological characteristics of strains of Colletotrichum gloeosporioides from citrus. Phytopathology 82:1377- 1382.   DOI
13 Chung, W., Ishii, H., Nishimura, K., Fukaya, M. and Yano, K. 2006. Fungicide sensitivity and phylogenetic relationship of anthracnose fungi isolated from various fruit crops in Japan. Plant Dis. 90:506-512.   DOI   ScienceOn
14 Kim, H. G. and Nam, M. H. 1999. Anthracnose of strawberry in Korea. Plant Dis. Agric. 5:8-13.
15 O'Neill, N. R., van Berkum, P., Lin, J. J., Kuo, J., Ude, G. N., Kenworthy, W. and Saunders, J. A. 1997. Application of amplified restriction fragment length polymorphism for genetic characterization of Colletotrichum pathogens of alfalfa. Phytopathology 87:745-750.   DOI   ScienceOn
16 Park, K. S. and Kim, C. H. 1992. Identification, distribution and etiological characteristics of anthracnose fungi of red pepper in Korea. Korean J. Plant Pathol. 8:61-69.
17 Weeds, P. L., Chakraborty, S., Fernandes, C. D., Charchar, M. J., Kexian, C. R. and Kelemu, S. 2003. Genetic diversity in Colletotrichum gloeosporioides from Stylosanthes spp. at centers of origin and utilization. Phytopathology 93:176-185.   DOI   ScienceOn
18 Von Arx, J. A. 1957. Die arten der Gattung Colletotrichum cda. Phytopathol. Z. 29:413-468.
19 Walter, J. M. 1992. Colletotrichum diseases of perennial and other cash crops. In: Colletotrichum-Biology, Pathology, and Control, ed. by J. A. Bailey and M. J. Jeger, pp.167-185. CAB International, Wallingford, United Kingdom.
20 Park, E. U., Hur, J. S. and Yun, S. C. 1992. A forecasting system for scheduling fungicide sprays to control grape ripe rot caused by Colletotrichum gloeosporioides. Korean J. Plant Pathol. 8:177-184.
21 Liyanage, H. D., McMillan, R. T. and Kistler, H. C. 1992. Two genetically distinct populations of Colletotrichum gloeosporioides from citrus. Phytopathology 82:1371-1376.   DOI