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http://dx.doi.org/10.5423/PPJ.OA.07.2018.0145

Ascospore Infection and Colletotrichum Species Causing Glomerella Leaf Spot of Apple in Uruguay  

Alaniz, Sandra (Department of Plant Protection, Faculty of Agronomy, University of the Republic)
Cuozzo, Vanessa (Department of Plant Protection, Faculty of Agronomy, University of the Republic)
Martinez, Valentina (Department of Plant Protection, Faculty of Agronomy, University of the Republic)
Stadnik, Marciel J. (Laboratory of Plant Pathology, Agricultural Science Center, Federal University of Santa Catarina)
Mondino, Pedro (Department of Plant Protection, Faculty of Agronomy, University of the Republic)
Publication Information
The Plant Pathology Journal / v.35, no.2, 2019 , pp. 100-111 More about this Journal
Abstract
Glomerella leaf spot (GLS) caused by Colletotrichum spp. is a destructive disease of apple restricted to a few regions worldwide. The distribution and evolution of GLS symptoms were observed for two years in Uruguay. The recurrent ascopore production on leaves and the widespread randomized distribution of symptoms throughout trees and orchard, suggest that ascospores play an important role in the disease dispersion. The ability of ascospores to produce typical GLS symptom was demonstrated by artificial inoculation. Colletotrichum strains causing GLS did not result in rot development, despite remaining alive in fruit lesions. Based on phylogenetic analysis of actin, ${\beta}$-tubulin and glyceraldehyde-3-phosphate dehydrogenase gene regions of 46 isolates, 25 from fruits and 21 from leaves, C. karstii was identified for the first time causing GLS in Uruguay and C. fructicola was found to be the most frequent (89%) and aggressive species. The higher aggressiveness of C. fructicola and its ability on to produce abundant fertile perithecia could help to explain the predominance of this species in the field.
Keywords
Colletotrichum fructicola; Colletotrichum karstii; Malus;
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1 Alaniz, S., Hernandez, L. and Mondino, P. 2015. Colletotrichum fructicola is the dominant and one of the most aggressive species causing bitter rot of apple in Uruguay. Trop. Plant Pathol. 40:265-274.   DOI
2 Carbone, I. and Kohn, L. M. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553-556.   DOI
3 Casanova, L., Hernandez, L., Martinez, E., Velho, A. C., Rockenbach, M. F., Stadnik, M. J., Alaniz, S. and Mondino, P. 2017. First report of glomerella leaf spot of apple caused by Colletotrichum fructicola in Uruguay. Plant Dis. 101:834.   DOI
4 Cheon, W., Lee, S. G. and Jeon, Y. 2016. First report on fruit spot caused by Colletotrichum gloeosporioides in Apple (Malus pumila Mill.) in Korea. Plant Dis. 100:210.   DOI
5 Damm, U., Cannon, P. F., Woudenberg, J. H. C., Johnston, P. R., Weir, B. S., Tan, Y. P., Shivas, R. G. and Crous, P. W. 2012. The Colletotrichum boninense species complex. Stud. Mycol. 73:1-36.   DOI
6 Gonzalez, E. and Sutton, T. B. 1999. First report of glomerella leaf spot (Glomerella cingulata) of apple in the United States. Plant Dis. 83:1074.2
7 Gonzalez, E. and Sutton, T. B. 2004. Population diversity within isolates of Colletotrichum spp. causing glomerella leaf spot and bitter rot of apples in three orchards in North Carolina. Plant Dis. 88:1335-1340.   DOI
8 Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33:1870-1874.   DOI
9 Guerber, J. C., Liu, B., Correll, J. C. and Johnston, P. R. 2003. Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility. Mycologia 95:872-895.   DOI
10 Hillis, D. and Bull, J. 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst. Biol. 42:182-192.   DOI
11 Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., Mc-Gettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A., Lopez, R., Thompson, J. D., Gibson, T. J. and Higgins, D. G. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23:2947-2948.   DOI
12 Liu, Y., Li, B., Wang, C., Liu, C., Kong, X., Zhu, J. and Dai, H. 2016. Genetics and molecular marker identification of a resistance to glomerella leaf spot in apple. Hort. Plant J. 2:121-125.   DOI
13 Sutton, T. B. and Shane, W. W. 1983. Epidemiology of the perfect stage of Glomerella cingulata on apples. Phytopathology 73:1179-1183.   DOI
14 MGAP-DIEA. 2016. Agricultural Statistical Yearbook 2016. URL: https://descargas.mgap.gub.uy/DIEA/Documentoscompartidos/Anuario2016/DIEA-Anuario2016cd.pdf [12 March 2019].
15 Paolocci, F., Rubini, A., Granetti, B. and Arcioni, S. 1999. Rapid molecular approach for reliable identification of Tuber spp. ectomycorrhizae. FEMS Microbiol. Ecol. 28:23-30.   DOI
16 Peever, T. L., Canihos, Y., Olsen, L., Ibanez, A., Liu, Y. C. and Timmer, L. W. 1999. Population genetic structure and host specificity of Alternaria spp. causing brown spot of Minneola tangelo and rough lemon in Florida. Phytopathology 89:851-860.   DOI
17 Perfect, S. E., Hughes, H. B., O'Connell, R. J. and Green, J. R. 1999. Colletotrichum: model genus for studies on pathology and fungal-plant interactions. Fungal Genet. Biol. 27:186-198.   DOI
18 Rockenbach, M. F., Velho, A. C., Goncalves, A. E., Mondino, P. E., Alaniz, S. M. and Stadnik, M. J. 2016. Genetic structure of Colletotrichum fructicola associated to apple bitter rot and glomerella leaf spot in southern Brazil and Uruguay. Phytopathology 106:774-781.   DOI
19 Sutton, T. B. and Sanhueza, R. M. V. 1998. Necrotic leaf blotch of golden delicious - Glomerella leaf spot: A resolution of common names. Plant Dis. 82:267-268.   DOI
20 Taylor, J. 1971. A necrotic leaf blotch and fruit rot of apple caused by a strain of Glomerella cingulata. Phytopathology 61:221-224.   DOI
21 Velho, A. C., Stadnik, M. J., Casanova, L., Mondino, P. and Alaniz, S. 2014. First report of Colletrotrichum karstii causing glomerella leaf spot on apple in Santa Catarina State, Brazil. Plant Dis. 98:157.
22 Woudenberg, J. H., Aveskamp, M. M., de Gruyter, J., Spiers, A. G. and Crous, P. W. 2009. Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype. Persoonia 22:56-62.   DOI
23 Velho, A. C., Alaniz, S., Casanova, L., Mondino, P. and Stadnik, M. J. 2015. New insights into the characterization of Colletotrichum species associated with apple diseases in southern Brazil and Uruguay. Fungal Biol. 119:229-244.   DOI
24 Wang, B., Li, B. H., Dong, X. L., Wang, C. X. and Zhang, Z. F. 2015a. Effects of temperature, wetness duration, and moisture on the conidial germination, infection, and disease incubation period of Glomerella cingulata. Plant Dis. 99:249-256.   DOI
25 Wang, C. X., Zhang, Z. F., Li, B. H., Wang, H. Y. and Dong, X. L. 2012. First report of glomerella leaf spot of apple caused by Glomerella cingulata in China. Plant Dis. 96:912.
26 Wang, W., Fu, D. D., Zhang, R. and Sun, G. Y. 2015b. Etiology of apple leaf spot caused by Colletotrichum spp. Mycosystema 34:13-25 (in Chinese).
27 Weir, B. S., Johnston, P. R. and Damm, U. 2012. The Colletotrichum gloeosporioides species complex. Stud. Mycol. 73:115-180.   DOI