The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia

  • Khaerani Nurlaelita (Phytopathology, Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Arif Wibowo (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Ani Widiastuti (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada)
  • Received : 2024.04.09
  • Accepted : 2024.06.10
  • Published : 2024.08.01
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Abstract

Dieback disease in mango trees has been observed in Indonesia, particularly in Java Island, with the causal agent remaining unidentified. One of the important pathogens that are responsible for causing mango dieback is Colletotrichum. Field surveys were conducted in various mango cultivating areas in Java Island, Indonesia to assess prevalence of Colletotrichum as dieback disease pathogen. Eleven Colletotrichum isolates were recovered from symptomatic dieback twigs and morphologically characterized. Genetic diversity fingerprint analysis was carried out using rep-PCR. Phylogenetic analysis identified isolates as belonging to Colletotrichum asianum and Colletotrichum cairnsense using partial sequences of four gene regions, including ITS, ACT, GAPDH, and TUB2. Pathogenicity tests on mango seedlings cv. Arumanis showed that all fungal isolates were responsible for causing dieback symptoms. Subsequently, symptomatic tissue was reisolated to fulfill Koch's Postulate. This study represented new funding for two species of Colletotrichum causing mango dieback in Indonesia.

Keywords

Acknowledgement

This study has been funded by the research grant of the Indonesian Endowment Fund for Education (LPDP)- Ministry of Finance, Indonesia. We thank Dr. Adyatma Wirawan Santosa, S.P., M.Sc. for technical support in phylogenetic tree analysis and Sri Giyanti for technical support in the laboratory. This report is a part of master thesis research conducted by the first author, under supervision corresponding and third author.

References

  1. Benatar, G. V., Wibowo, A. and Suryanti. 2020. First report of Colletotrichum asianum associated with mango fruit anthracnose in Indonesia. Crop Prot. 141:105432.
  2. Choi, Y.-W., Hyde, K. D. and Ho, W. W. H. 1999. Single spore isolation of fungi. Fungal Divers. 3:29-38.
  3. da Silva, L. A. B., Leite, D. M. and Capucho, A. S. 2022. Chemical control of dieback and mango malformation in a semiarid region. Agric. Parasitol. 89:e00542020.
  4. de Silva, D. D., Ades, P. K., Crous, P. W. and Taylor, P. W. J. 2017. Colletotrichum species associated with chili anthracnose in Australia. Plant Pathol. 66:254-267.
  5. Hassan, A. H., Bebawy, A. S., Saad, M. T., Mosaad, G. S., Saad, B. T., Eltayeb, W. N. and Aboshanab, K. M. 2022. Metagenomic nanopore sequencing versus conventional diagnosis for identification of the dieback pathogens of mango trees. BioTechniques 73:261-272.
  6. Li, Q., Bu, J., Shu, Juan., Yu, Z., Tang, L., Huang, S., Guo, T., Mo, J., Luo, S., Solangi, G. S. and Hsiang, T. 2019. Colletotrichum species associated with mango in southern China. Sci. Rep. 9:18891.
  7. Mayorquin, J. S., Nouri, M. T., Peacock, B. B., Trouillas, F. P., Douhan, G. W., Kallsen, C. and Eskalen, A. 2019. Identification, pathogenicity, and spore trapping of Colletotrichum karstii associated with twig and shoot dieback in California. Plant Dis. 103:1464-1473.
  8. Patricia, L.-C. C., del Socorro, R.-G. M., Ivan, R.-H., Erika, D. L. C.-A., Carolina, D. S., Keiko, S. and Alberto, N.-Z. J. 2021. Occurrence and infective potential of Colletotrichum gloeosporioides isolates associated to Citrus limon var Eureka. Biotechnol. Rep. 31:e00651.
  9. Pramunadipta, S., Widiastuti, A., Wibowo, A. and Priyatmojo, A. 2022. rep-PCR analysis of Fusarium proliferatum causing sheath rot disease and its relationship to light, pH, temperature, and rice varieties. Arch. Phytopathol. Plant Prot. 55:973-990.
  10. Prihastuti, H., Cai, L., Chen, H., McKenzie, E. H. C. and Hyde, K. D. 2009. Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Divers. 39:89-109.
  11. Ramos, A. P., Talhinhas, P., Sreenivasaprasad, S. and Oliveira, H. 2016. Characterization of Colletotrichum gloeosporioides, as the main causal agent of citrus anthracnose, and C. karstii as species preferentially associated with lemon twig dieback in Portugal. Phytoparasitica 44:549-561.
  12. Saeed, E. E., Sham, A., AbuZarqa, A., Al Shurafa, K. A., Al Naqbi, T. S., Iratni, R., El-Tarabily, K. and AbuQamar, S. F. 2017. Detection and management of mango dieback disease in the United Arab Emirates. Int. J. Mol. Sci. 18:2086.
  13. Savant, N. V. and Raut, S. P. 2000. Studies on symptomatology of die-back of mango stone grafts. Acta Hortic. 509:823-832.
  14. Sharma, G. and Shenoy, B. D. 2016. Colletotrichum systematics: past, present and prospects. Mycosphere 7:1093-1102.
  15. Siddiquee, S. 2017. Practical handbook of the biology and molecular diversity of Trichoderma species from tropical regions. Fungal biology. Springer, Cham, Switzerland, pp. 41-73.
  16. Uysal, A., Kurt, S. and Guarnaccia, V. 2022. Distribution and characterization of Colletotrichum species associated with Citrus anthracnose in eastern Mediterranean region of Turkey. Eur. J. Plant Pathol. 163:125-141.
  17. Vitale, A., Alfenas, A. C., de Siqueira, D. L., Magista, D., Perrone, G. and Polizzi, G. 2020. Cultivar resistance against Colletotrichum asianum in the world collection of mango germplasm in Southeastern Brazil. Plants 9:182.
  18. Wang, W., de Silva, D. D., Moslemi, A., Edwards, J., Ades, P. K., Crous, P. W. and Taylor, P. W. J. 2021. Colletotrichum species causing anthracnose of citrus in Australia. J. Fungi 7:47.
  19. Widiastuti, A., Suryanti, Giovanni, A. C., Santika, I. A. and Paramita, N. R. 2023. Fungal community associated with mixed infection of anthracnose and stem end rot diseases in Chokanan Mango. Biodiversitas 24:2163-2170.