• 한국환경과학회지
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• 제25권4호
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• pp.579-588
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• 2016

본 연구에서는 홍어껍질에서 추출한 콜라겐 펩타이드 이화학적 특성과 비만 유발 쥐에 대한 투여 실험을 통해서 지방조직의 지질 감소효과를 조사하였으며, 홍어 껍질 콜라겐 펩타이드를 원료로 한 응용 제품 개발을 통하여 산업적 적용을 검토하였다. 일반성분 분석 결과 홍어 껍질 콜라겐 펩타이드는 대부분이 단백질로 이루어져 있는 고단백 저지방 물질이며, 분자량 측정 결과 약 1000 Da.의 저분자량이고, 아미노산 분석에서는 전형적인 콜라겐 단백질의 특성을 나타내었다. 비만 유발 쥐에 홍어 껍질 콜라겐 펩타이드를 500 mg/kg씩 18일간 경구 투여한 실험 결과에서 사료 섭취량과 음수량이 상당량 줄어 들었으며, 지방조직의 중성지방과 총 콜레스테롤 수치가 유의적으로 감소하였다. 홍어 껍질 콜라겐 펩타이드를 대량생산을 한 결과 제품의 최종 수율은 원료대비 약 17.23%로 상당히 높은 수율을 나타내었다. 이를 원료로 한 기능성 화장품 및 생활용품을 생산 및 출시하였다. 따라서 홍어 가공 부산물인 껍질을 재이용한 기능성 단백질 소재 개발을 통하여 건강기능식품과 화장품 분야에서의 기능성과 가격경쟁력을 갖춘 소재 개발에 기여 할 것으로 기대된다.

• 대한한의학회지
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• 제37권2호
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• pp.12-22
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• 2016

Objectives: The aim of this study was to confirm the dose-dependent skin moisturizing effects of dried pomegranate concentrate powder (PCP) and pomegranate concentrate solution (PCS) in ICR mice. Materials and methods: To observe the in vivo skin moisturizing effects and possible underlying mechanisms of PCP and PCS, oral PCP (100, 200, and 400 mg/kg) and PCS (1, 2, and 4 mL/kg) were administered to normal ICR mice. Changes in body weight, skin water content, and skin type I collagen and hyaluronan contents were measured. Additionally, the mRNA expression levels of hyaluronan synthase (Has) 1, 2, and 3, and collagen type I alpha (COL1A) 1 and 2 were determined in the dorsal skin of mice by real-time reverse transcription polymerase chain reaction (RT-PCR). Results: Significant and dose-dependent increases in dorsal skin water content and type I collagen and hyaluronan contents were seen in PCP and PCS-treated mice. Moreover, the mRNA levels of Has 1, 2, and 3, involved in hyaluronan synthesis, and of COL1A1 and COL1A2, involved in collagen synthesis, were significantly and dose-dependently upregulated in PCS- and PCP-treated mice. Conclusions: In this study, PCP and PCS led to favorable skin moisturizing effects as indicated by increased skin water content and the upregulation of hyaluronan and collagen synthesis enzymes in mice treated with PCS (4 mL/kg) and PCP (200 mg/kg).

• 한국식생활문화학회지
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• 제21권2호
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• pp.161-165
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• 2006

소뼈의 효과적인 이용을 위하여 사골뼈와 갈비뼈의 가열시간에 따른 칼슘, 인, 마그네슘, 단백질, 총 유리 아미노산, 콜라겐 등의 영양성분 측정과 관능검사를 실시한 결과는 다음과 같다. 12시간 까지 가열할 경우, 조리시간이 경과할수록 고형물, 칼슘, 인, 마그네슘, 단백질, 총 유리아미노산, 콜라겐 함량은 점점 증가하였으며 12시간 가열에서 가장 높았으며, 이와 같은 영양성분이 사골뼈 보다 갈비뼈에서 조금 높게 용출되었다. 12시간 끓인 시료에 식염 0.8%를 첨가하여 관능 검사를 실시한 결과 색, 향기, 맛, 종합적인 맛에서 갈비뼈 시료가 사골뼈 시료보다 높은 기호도를 나타냈다. 이상의 결과들로부터 사골뼈와 갈비뼈를 12시간 가열함으로써 칼슘과 마그네슘 등 영양성분이 가용화되기 쉬우며, 사골뼈와 갈비뼈의 영양성분과 맛이 우수하므로 다양한 이용과 개발을 시사하였다.

• 한국수산과학회지
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• 제55권4호
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• pp.397-407
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• 2022

This study investigated the collagen and texture properties of commonly consumed sliced raw fish species (CC-SRF) [olive flounder (OF), red seabream (RS), Atlantic salmon (AS), coho salmon (CoS) and sockeye salmon (SS)] distributed in Korea as sliced raw fishes. The crude lipid contents of CC-SRF were 5.5% for OF, 6.8% for RS, 18.5% for AS, 16.1% for CoS, and 5.7% for SS. The collagen content and solubility from CC-SRF were 622 mg/100 g and 78.0%, respectively, in OF, 270 mg/100 g and 75.6%, respectively, in RS, 237 mg/100 g and 24.1%, respectively, in AS, 341 mg/100 g and 65.7%, respectively, in CoS, and 246 mg/100 g and 17.9%, respectively, in SS. The texture of CC-SRF was affected by the lipid content, collagen content, acid solubility, hydroxylation, and cross linkage degree. The highest hardness of CC-SRF was obtained from OF, followed by RS, SS, AS and CoS. There was, however, no difference (P>0.05) in hardness between OF and RS and between AS and CoS.

• 분석과학
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• 제28권6호
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• pp.361-369
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• 2015

골관절염에 대한 참당귀 추출분말 (AGE)의 치료효과를 검토하고자 토끼연골세포와 흰쥐의 monosodium iodoacetate (MIA)로 유발된 골관절염 부위에서 시료를 채취하여 MMPs의 발현에 대한 AGE의 억제 효능을 검토하였다. 고 농도의 AGE (50 μg/mL) 투여에서 세포독성은 관찰되지 않았으며 면역 및 염증반응과 관련된 여러 인자의 전사인자인 NF-κB 활성화를 효과적으로 억제시켰다. 토끼연골 세포에서 MMP-2와 MMP-9의 활성을 확인해본 결과 AGE는 MMP-9의 활성을 효과적으로 억제시키는 것을 확인하였다. AGE를 토끼연골세포에 처리하여 분석한 결과, 주요성분인 decursin과 decursinol angelate가 3.62±0.47 μg/mg protein와 2.14±0.36 μg/mg protein으로 검출되었다. 동물실험을 위하여, 골관절염은 MIA를 흰쥐 무릎관절에 처리하여 동물모델을 만들었으며, 매일 25, 50와 100 mg/kg의 AGE를 3주 동안 먹인 결과 흰쥐의 연골 조직에서 MMPs가 억제되는 것을 확인하였다. 연골조직으로부터 RT-PCR을 통해 collagen Type I, collagen Type II, aggrecan 및 MMPs (MMP-3, MMP-9, MMP-13)의 mRNA를 확인해본 결과 AGE는 collagen Type I, collagen Type II, aggrecan은 증가시키며 MMPs는 감소시키는 효과를 얻었다. 결론적으로 AGE는 MMPs 억제를 통하여 골관절염의 발생을 억제한다.

• Natural Product Sciences
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• 제19권4호
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• pp.303-310
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• 2013

Velvet deer antler (VDA) is well known oriental medicine claimed to have tonic activities as improving bone mineral density (BMD), immune-enhancing, rejuvenating and many other medicinal activities. Ossified deer antler (ODA) is bony product produced by over-calcification of deer antler due to late harvesting. The extraction efficiency of ODA by conventional boiling in water must be very poor due to bony nature, hence the reputations for the medicinal efficacies of ODA has been highly under-evaluated compared to that of VDA without any experimental evidences. Employing our new efficient water extraction process ($135^{\circ}C$), the extracts of ODA and VDA were analysed to compare the contents of bioactive components and the potencies of pharmacological activities. The results showed that; 1) The $135^{\circ}C$ extraction (autoclaving) of ODA gave highly increased amount of biomass, 120% more than the conventional extraction by 100-boiling, whereas the same treatment for VDA showed only 15% increased amount of biomass. 2) Feeding the ODA- or VDA-extracts to oophorectomized rats showed very potent BMD-recovering activity. 3) During the ossification of deer antler, the total collagen content was found to be increased by addition of type-1 to pre-existing type-2 collagen, but not replacement of type-2 to type-1 collagen. High titer of peptide hormones like growth hormone and IGF-1 were detected in the ODA- and VDA-extracts and also in the serum of ODA- or VDA-treated oophorectomized animals dose-dependently. Present experimental data will give a conclusion that folkloric poor reputations on ODA must be concerned only with poor extraction efficiency of conventional $100^{\circ}C$ water extraction and not based on the composition of bioactive substances of ODA.

• 한국축산식품학회지
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• 제37권4호
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• pp.486-493
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• 2017

The effects of slaughter age (28, 32, or 38 mon) on the proximate composition, collagen content, fatty acid composition, amino acid content, and mineral contents of horse leg bone (femur and tibia) extracts (HLBE) obtained from Jeju crossbred horses (n=14) in Korea. HLBE was extracted for 24 h with boiling water. At those ages, the respective proximate compositions of the HLBE were found as follows: 5.20-6.42% crude protein; fat 0.61-1.65% crude; and 0.10-0.22% crude ash. At 32 and 38 mon, the HLBE showed higher levels of both crude protein and collagen than at 28 mon. The major fatty acids of the horse leg bone extract powder (HLBP) were palmitic acid and oleic acid. Palmitoleic acid and the essential fatty acids were higher in the HLBP at 38 mon compared to that at 28 mon. Nearly all the amino acids were found at higher levels in the HLBP at 38 mon than at 28 mon, except histidine. The P, K, Zn, Se and Fe contents in the HLBP increased significantly with age. These results suggest that some nutrients in the HLBE increase with age, and that extracts from horses older than 32 mon would be more nutritious for human consumers.

• 대한본초학회지
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• 제28권3호
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• pp.39-44
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• 2013

Objectives : The purpose of this study was to identify active fraction and components from antiplatelet Ulmi cortex extract. Methods : The 70% ethanol extract of Ulmi cortex was subjected to column chromatography over D101 resin and eluted with an 20% (W1), 30% (W2), 40% (W3), 50%(W4), 70% (W5), and 100% ethanol (W6) to yield 6 fractions. W6 was further fractioned and its active components were purified using semi-preparative HPLC. The isolated compounds were identified by MS and NMR, and their contents were simultaneously analyzed using HPLC/UV. Antiplatelet aggregation activities of the fractions and the compounds were evaluated using rat platelet-rich plasma in presence of collagen ($5{\mu}g/ml$), arachidonic acid (0.05 U/ml), or thrombin ($100{\mu}M$). Results : Among six fractions, W3 prominently inhibited platelet aggregation. At the concentration of $200{\mu}g/ml$, W3 strongly inhibited arachidonic acid- and collagen-induced platelet aggregations by 78.2% and 65.9%, respectivley, and weakly inhibited thrombin-inducded platelet aggregation by 32.6%. Catechin, epicatehin, and catechin-7-O-${\beta}$-D-glucopyranoside were isolated from W3 and their contents were revealed to be 15.1%, 0.87%, and 0.32%. Catechin and epicatechin at the concentrations of $100{\mu}M$ strongly inhibited collagen-induced platelet aggregation by 79.9% and 86.6%, respectively, but weakly inhibited arachidonic acid- and thrombin-induced platelet aggregations. Conclusions : A main active principle of anitplatelet Ulmi Cortex extract is W3 fraction, of which main active component is catechin considering its antiplatelet activity and content.

• 한국축산식품학회지
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• 제42권5호
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• pp.833-848
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• 2022

The objective of this study was to characterize the meat quality traits that affect the texture and savory taste of Ufipa indigenous chickens by comparing the proximate composition, physical characteristics, collagen, and nucleic acid contents with those of commercial broilers. It was found that Ufipa chicken breast and thigh meat had a higher protein content (p<0.05) than broiler chicken meat, whereas the fat content was lower (p<0.01). The moisture content of thigh meat was lower in Ufipa chicken meat than in broiler chicken meat (p<0.05). Regarding meat color, broiler chickens had considerably higher L^* and b^* than Ufipa chickens in both the breast and the thigh meat, except for a^* (p<0.01). Regarding water holding capacity, Ufipa chicken breast exhibited higher drip loss but lower thawing and cooking losses than broiler chicken (p<0.01). In contrast, its thigh meat had a much lower drip and thawing losses but higher cooking losses (p<0.01). The shear force of Ufipa chickens' breasts and thighs was higher than that of broiler chickens (p<0.05), while the amount of total collagen in the thigh meat was higher than that of broiler chickens (p<0.05). Additionally, the inosine-5'-monophosphate (IMP) of Ufipa chicken breast and thigh meat was higher than that of broiler meat (p<0.05). The principal component analysis of meat quality traits provides a correlation between the proximate and physical-chemical prosperties of both breeds with some contrast. In conclusion, the present study provides information on healthy food with good-tasting Ufipa indigenous chickens, which offer a promising market due to consumers' preferences.

• Asian-Australasian Journal of Animal Sciences
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• 제11권3호
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• pp.300-306
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• 1998

An experiment was conducted to investigate correlations of litter size and average serum progesterone concentrations during pregnancy with mammary gland growth and development at parturition. Twenty ewes (5, 9, 4, and 2 ewes carrying 0, 1, 2, and 3 lambs, respectively) were used to measure weekly serum progesterone concentration during pregnancy. At parturition, the experimental ewes were slaughtered for determination of mammary gland growth and development at parturition (mammary dry fat-free tissue [DFFT], DNA, RNA, collagen, protein, and glycogen). Correlation of mammary DFFT with litter size and averages serum progesterone concentrations were 0.75 and 0.72, respectively. Litter size or maternal serum progesterone concentrations did not correlate with the mammary DNA concentration. However, litter size or maternal serum progesterone concentrations positively correlated (p < 0.01) with the mammary RNA and protein concentrations, but negatively correlated with the mammary collagen (p < 0.01) and. glycogen (p < 0.05) concentrations. Litter size or maternal serum progesterone positively correlated (p < 0.01) with the total mammary DNA, RNA, collagen, protein and glycogen contents. These results implied that the increased concentrations of progesterone with the increased litter size during pregnancy improved mammary gland growth and development at parturition.