• 생명과학회지
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• 제20권11호
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• pp.1634-1639
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• 2010

본 연구는 무지개송어 양식 산업의 생산성 향상을 위해 전 암컷 무지개송어 대량생산을 위한 일환으로 성호르몬에 의한 생리학적 성전환과 자성발생 2배체어를 유도하였다. 생리학적으로 성전환된 수컷을 만들기 위하여 부화 후 첫 먹이를 먹는 시기에 웅성호르몬인 17 alpha-methyltestosterone (MT) 5 mg을 사료에 흡착시켜 사육수온 $13^{\circ}C$에서 적산수온 $800^{\circ}C$까지 처리한 결과 96.7%의 수컷 유도율을 보였다. 또한 정상 수컷 정액을 이용하여 수정 10분 후 $28^{\circ}C$에서 20분간 고온 처리하여 61.7%의 자성발생 2배체가 유도되었다.

• The Korean Journal of Pain
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• 제24권4호
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• pp.185-190
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• 2011

Background: Spinal nerve ligation (SNL) injury in rats produces a pain syndrome that includes mechanical and thermal allodynia. Previous studies have indicated that proinflammatory cytokines such as tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) play an important role in peripheral mediation of neuropathic pain, and that altered dorsal root ganglion (DRG) function and degree of DRG neuronal apoptosis are associated with spinal nerve injury. The present study was conducted to evaluate the expression of TNF-${\alpha}$ and the extent of apoptosis in the dorsal root ganglion after SNL in rats. Methods: Sprague-Dawley rats were subjected to SNL of the left L5 and L6 spinal nerves distal to the DRG and proximal to the formation of the sciatic nerve. At postoperative day 8, TNF-${\alpha}$ protein levels in the L5.6 DRG were compared between SNL and naive groups using ELISA. In addition, we compared the percentage of neurons injured in the DRG using immunostaining for apoptosis and localization of activated caspase-3. Results: SNL injury produced significant mechanical and cold allodynia throughout the 7-day experimental period. TNF-${\alpha}$ protein levels were increased in the DRG in rats that had undergone SNL ($12.7{\pm}3.2$ pg/100 ${\mu}g$, P < 0.001) when compared with naive rats ($4.1{\pm}1.4$ pg/100 ${\mu}g$). The percentage of neurons or satellite cells co-localized with activated caspase-3 were also significantly higher in rats with SNL than in naive rats (P < 0.001, P < 0.05, respectively). Conclusions: SNL injury produces mechanical and cold allodynia, as well as TNF-${\alpha}$ elevation and apoptosis in the DRG.

• 한국지역사회생활과학회지
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• 제16권1호
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• pp.149-163
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• 2005

Family has emerged as a key concept for health, and it has been identified as one of the most important conditions. The relationship between health habit and its management is different depending on family. The odd pair family, mostly rural lower income class, worry to have poor health because of no spouse and small family size. One thousand eight hundred and seventy(1870) subjects were collected in 9 provinces through the sampling of Probability Proportional to Size (PPS). Questionnaire method was conducted on health checking, bath states, alcohol consumption, cigarette smoking, and the prevalence of farmer's health related problems. The main results were as follows: 1) The characteristics of odd pair families are that the head of household is female(77% ), the size of family is small(1.76 persons), the education level is low(7.5 years for male, 3.1 years for female) and the age group is old (male: 89.78 year old, female: 73.69 year old). 2) For the odd pair family, the frequency of health checking is quite low with one or two times per year(l0.2%) and the rate of no-health checking is much higher(35.8%) .3) Bathing utility is not available 29.6% of the odd pair family and only cold water is supplied at home for the 11.5 % of them. However, for the paired family, 9.8 % of them has no bathing utility and the rate of the family supplied with only cold water is just 7.9%. 4) The bathing frequency score of odd pair family is l.74points for male and 1.25 points for female. 5) The rate of smoking habits for odd pair family is 68.5 % and specially it is 7.6% for female, which is higher comparing with that of pair family. 6) The smoking frequency score of odd pair family is 1.57 points. 7) Alcohol drinking frequency score of odd pair family is 1.79 points for male, and 3.24points for female. 8) Farmers' syndrome(FS) revealed 38.7% of odd pair family and it is lower than that of pair family(57.3%). Special pain of FS was huckle bone and muscle(28.4%) and articular pain(24.l %). The pain rate of huckle bone and muscle(43.l %) and articular pain(33.5%) were higher in a year in odd pair family were lower than those of pair family: farming machine caused accidents(6.5%) and pesticide poisoning(5.7%). l0) The odd pair family use more frequently medical clinic or public health center for the treatment of FS(74.7%) and pesticide poisoning(62.5%) than the pair family for FS(69.0%) and for pesticide poisoning(.53.6%). The score of FS treatment is 5.70 points for odd pair family and it is not significantly different from 5.62 points of the paired family. The result of pesticide poisoning treatment score is as same as that of FS.

• 한방안이비인후피부과학회지
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• 제11권1호
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• pp.23-39
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• 1998

The clinical data and thermographic imaging were analyzed on the 100 cases of Bell's palsy who were treated in the Kwang-Ju Oriental Medical Hospita! of Wonkwang University from February to October 1997. All the cases were taken Digital Infrared Thermograph Imaging(DITI) before treatment and 30 cases of them were taken follow up imaging again after recovery. And the following results were obtained. 1. Of 100 cases, under teenager occupied $1\%,\;teenager\;8\%,\;2nd\;decade\;14\%,\;3rd\;decade\;11\%,\;4th\;decade\;16\%,\;5th\;decade\;26\%,\;6th\;decade\;16\%,\;7th\;decade\;7\%\;and\;over\;80\;occupied\;1\%$. 2. Male occupied $48\%$ and female occupied $52\%$. 3. $42\%\;of\;male\;and\;23\%$ of female had the affected side at left side. And right facial nerve palsy occured at $29\%\;of\;male\;and\;29\%$ of female. 4. The most common cause of Bell's palsy was cold wind $18\%$, and the next were excessive labor $15\%,\;stress\;12\%,drinking\;2\%\;and\;cold\;food\;1\%$. 5. Thermal pattern were present as hyperthermal pattern in $44\%$ and hypothermal pattern in $22\%$. 6. The DITI showed hyperthermal pattern as close as to the onset day and changes to hypothermal pattern as times passed. 7. The DITI pattern and post-auricular pain, the most common prodomal syndrome, showed no significant relationship. 8. The relationship between the grade of paralysis and thermal patttern of DITI showed no significance. 9. Mean temperature of loci in affected side indicrtted $30.27^{\circ}C\;at\;Yang\;baek,\;30.02^{\circ}C\;at\;Taeyang,\;29.25^{\circ}C\;at\;Geoyo,\;29.62^{\circ}C\;at\;Jichang,\;29.78^{\circ}C\;at\;Hakwan,\;29.61^{\circ}C\;at\;Hyupgeo,\;and\;30.59^{\circ}C$at Yeopoong. 10. Mean temperature of loci in unaffected side showed $30.16^{\circ}C\;at\;Yang\;baek,\;30.02^{\circ}C\;at\;Taeyang,\;29.61^{\circ}C\;at\;Geoyo,\;29.68^{\circ}C\;at\;Jichang,\;29.70^{\circ}C\;at\;Hakwan,\;29.57^{\circ}C\;at\;Hyupgeo,\;and\;29.89^{\circ}C$at Yeopoong. 11. Of 30 cases who were taken follow up imaging again after recovery, the relationship between delta T at loci and symptoms showed no significance. It should be needed further investigation in order to apply them for clinical evaluation.

• 사상체질의학회지
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• 제12권1호
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• pp.110-119
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• 2000

1. 연구목적(硏究目的) 이제마(李濟馬) 선생께서 함흥에서 의업(醫業)을 열어 사상의학(四象醫學)을 제창한 지 100여 년이 지났으나 이제마(李濟馬) 선생께서 쓰신 약물(藥物)에 대한 연구(硏究)가 아직 정립(定立)이 되지 않아 기존(旣存)의 음양오행론적(陰陽五行論的) 관점(觀點)에서 사상(四象) 처방(處方)을 쓰고 있는 것이 현실이다. 이 론고(論考)는 소양인(少陽人)의 대표적 약물인 석고(石膏)를 "동의수세보원(東醫壽世保元)"에서 이병증약(裏病證藥)이라고 규정하면서도 비수한표한병증(脾受寒表寒病證) 처방(處方)에도 사용되는 예가 있어 표이병증(表裏病症)에서 쓰이는 용례(用例)와 올바를 수치법(修治法), 귀경(歸經), 소양인(少陽人)의 한열병증(寒熱病症)에서의 역할을 살펴보아 소양인(少陽人) 비수한표한병(脾受寒表寒病)에서 사용된 석고(石膏)의 운용(運用) 정신(精神)을 살펴보고자 하였다. 2. 연구방법(硏究方法) 역대(歷代) 본초(本草) 의서(醫書)와 동의보감(東醫寶鑑)에 나타남 기존(旣存) 음양오행론적(陰陽五行論的) 관점(觀點)에서 석고(石膏)의 본초론적(本草論的) 의미를 살펴보고 동의수세보원(東醫壽世保元)의 소양인(少陽人) 비수한표한병(脾受寒表寒病)의 망음증(亡陰證) 처방(處方)에서 석고(石膏)가 포함된 처방(處方)인 형방사백산(荊防瀉白散)과 저령동전자탕(猪笭東前子湯)과 같은 망음증(亡陰證) 처방(處方)이면서 석고(石膏)가 들어가지 않은 골석고삼탕(滑石苦參湯)과 형방지황탕(荊防地黃湯)의 두 처방군(處方群)을 비교하여 석고(石膏)가 소양인(少陽人) 비수한표한병(碑受寒表寒病)에 쓰이게 된 운용(運用) 정신(精神)을 살펴보고 소양인(少陽人) 이병증(裏病證)에서 석고(石膏)의 효능(效能)과 작용(作用) 부위(部位)를 살펴보아 소양인(少陽人) 한열병증(寒熱病證)에서의 석고(石膏)의 효용(效用)에 대해 알아보았다. 3. 고찰(考察) 및 결과(結果) 이상의 과정을 통하여 다음과 같은 결론(結論)을 얻을 수 있었다. 1. 석고(石膏)의 수치법(修治法)은 감초수비(甘草水飛), 화가(火假)의 방법이 있으나 소양인약(少陽人藥)은 포, 자(炙), 초(炒), 법(法)을 사용하지 않고 소양인(少陽人) 병증(病症)에서의 석고(石膏)의 효용이 청열사화(淸熱瀉火)하는 기능이 강하므로 생석고(生石膏)를 감초수비(甘草水飛)하여 연세(硏細)하여 사용하는 것이 적절하다. 2. 석고(石膏)가 표병증약(表病證藥)인 형방사백산(荊防瀉白散)과 저령동전자탕(猪笭東前子湯)에 사용되는 양병(養病)이 같이 겸하여 표양겸병(表養兼病)에 청양열이강표음(淸養熱而降表陰)하는 치법(治法)에 사용되는 것이다. 3. 소양인(少陽人) 비수한표한병(脾受寒表寒病) 중에 망음증(亡陰證)은 신열두통설사(身熱頭痛泄瀉)의 망음(亡陰)과 신한복통설사(身寒腹痛泄瀉)의 망음증(亡陰證)으로 구별되며 신열두통설사(身熱頭痛泄瀉)의 망음증(亡陰證)에만 석고(石膏)가 쓰이게 되며 설사(泄瀉)의 유무(有無)와는 관계없이 신열두통(身熱頭痛)의 증장(證狀)으로 망음증(亡陰證)으로 전변(轉變)되는 증상(證狀)이 있을 때는 석고(石膏)를 사용하게 된다. 4. 석고(石膏)는 화열병증(火熱病證)으로 양화(陽化)되기 쉬운 소양인(少陽人)의 체질적(體質的) 특징(特徵)을 청열사화(淸熱瀉火)의 작용으로 소양인(少陽人) 보명지주(保命之主)인 음청지기(陰淸之氣)를 도와주는 대표적인 약물이다. 5. 석고(石膏)의 작용(作用) 부위(部位)는 중상초(中上焦) 부위(部位)인 위국(胃局)으로 위국(胃局)의 모양(耗陽)을 청열사화(淸熱瀉火)하여 소양인(少陽人) 이병증(裏病證)의 대전제(大前提)인 대변폐(大便閉)의 증상을 해소하여 청양(淸陽)을 상달(上達)시키는 작용을 한다.

• 대한한방내과학회지
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• 제23권2호
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• pp.280-285
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• 2002

Pruritus is [CHECK DEFN] itchy feeling of one's skin. We often face stroke patients who complain about Pruritus. They occasionally fail to fall asleep or have a secondary infection as a result of scratching. For these reasons, severe Pruritus brings down general condition and interferes with recovery. The causes of Pruritus are distributed to from skin diseases and from internal diseases. Especially among the skin diseases, Xerotic Eczema, which is called Senile Eczema causes the dry skin in the elderly, especially lower limb's extensor part. According to the epidemiology, 20% of the old have Xerotic Eczema. In oriental medicine, Pruritus is called Pungsoyang(風瘙痒), Pungyang(風痒), Yangpung(痒風), Sinyang(身痒). The cause of Pruritus is divided into two. One is endogenous factors and the other is exogenous factors. The former are deficiency of blood(血虛), blood fever(血熱), wind-heat due to internal damage(內傷風熱), damp-heat in the liver and gallbladder(肝膽濕熱), endogenous wind stirring in the liver(肝風內動), deticiency syndrome of the spleen(脾虛), deficiency of Yin of the liver and kidneys(肝腎陰虛) and deficiency of the Penetration and Conception Vessels(衝任不足). The latter are wind-cold due to exogenous affection(外感風寒) and wind-heat due to exogenous affection(外感風寒). We report two stroke patients who complained of severe Pruritus They were diagnosed as having Xerotic Eczema by a dermatologist. We regarded their Pruritus as blood fever(血熱) and wind-heat(風熱) and prescribed Sopung-San to these patients. These patients showed significant improvement.

• 대한한의학원전학회지
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• 제19권2호통권33호
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• pp.248-265
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• 2006

Impulse of ki refers to palpitation around the navel, it is mostly caused by impairment of yang due to erroneous sweating, deficiency of the spleen yang. Behcet's syndrome caused by prolonged damp-heat and pathogenic germs. Its symptoms are characterized by conjunctival congestion, blue canthus and ulceration of the oral cavity, restlessness all the time, etc. It is advisable to follow the therapeutic principles of clearing away heat and drying damp, detoxicating and sterilizing. Lily disease is one of the emotional disease. It caused by yin deficiency of the heart and lung, found in mental depression or convalescence after a critical disease. Its symptoms are characterized by restlessness, reticence, insomnia, failure to walk, poor appetite, a subjective hot or cold, bitter taste and dark urine. It is advisable to follow the therapeutic principle of nourishing yin to clear away heat. Cholera refers to the disease marked by sudden severe vomiting and diarrhea, and colic of the heart and the abdomen. It is mostly caused by deficiency of the ki in the middle-energizer, emotional stress, etc. It is characterized by such symptoms as sudden severe vomiting and diarrhea, restlessness, etc. It is advisable to follow the therapeutic principles of warming yang and removing toxic substances, and strengthening the spleen and stomach. Heat invasion of blood chamber due to affection of the exterior pathogens, pathogenic heat invading the blood in deficiency and fighting with the blood. Its symptoms are marked by fever, feeling of the fullness, etc. There are deficiency of blood, hot blood and stasis of blood. The different therapeutic treatment should be taken according to different types thereof.

• 대한한의학원전학회지
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• 제23권2호
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• pp.235-270
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• 2010

This paper studies the main treatment methods, Ikgi(益氣) and Seung-yang(升陽) applied frequently by Lee Jema(李濟馬) on the exterior disease of So-eumin(少陰人), one of the four constitutions. The methodology of this paper is to understand the pharmacology of "Dong-uisusebowon(東醫壽世保元)" by examining the formulas applied by Lee Jema. I have examined the organization of formulas in "Dong-uisusebowon(東醫壽世保元)" beforehand to categorize the formulas applicable in this study according to an objective standard. I have analyzed the prescriptions applied to So-eumin exterior disease. As a result, I could see that in the case of Ulgwang(鬱狂) syndrome, Ikgi(益氣) and Seung-yang(升陽) methods were mainly applied, but as the disease progressed, Seung-yang(升陽) was withdrawn while Ikgi(益氣) was stressed. Likewise, in the case of Mang-yang(亡陽), both methods were mainly adopted, but as the patients got worse, the level of Seung-yang(升陽) was maintained and that of Ikgi(益氣) was elevated with the addition of Buja(附子). Through this process, we could verify the overall action of Ikgi(益氣), Seung-yang(升陽) and Buja(附子). Originally, the two methods of Ikgi(益氣) and Seung-yang(升陽) are intimately related, but by analyzing the overall functions of the two methods, we could see that Seung-yang(升陽) and Buja(附子) support the Yang gi of the interior of So-eumin, while Ikgi(益氣) resolves inner stagnation of Yang gi and emits the cold pathogen of the exterior. Also, in the course of treatment, Ikgi(益氣) could only be realized after securing Seung-yang(升陽).

• 대한한방부인과학회지
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• 제18권4호
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• pp.119-135
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• 2005

Purpose : This study was investigated, in order to improve that reliability of disease mechanism diagnosis, which were used for the object diagnosis of Oriental medicine in the dept. of Oriental OB&GY, Oriental Medical hospital of Dong-Eui University, amend to a disease mechanism item of Questionnaires and indexes of disease mechanism, we were thought that the results were more pointed to the condition of appropriate disease mechanism, as a result of put a results of Questionnaires and belongs to indexes of disease mechanism together, we　suggested to call it a　'weight of disease mechanism', because It was possible to　mark a different degrees of indexes of disease mechanism in the same points.　Methods : We analyzed the results of Questionnaires about 3354 outpatients who　had OB&GY disease in the Oriental Medical hospital of Dong-Eui University from April 2000 to March 2004. Results : 1. weight of disease mechanism is 10 score according to disease mechanism score and the standard of reliability. 2. The standard of reliability is same 11 disease mechanism item except stagnated blood, cold syndrome, dry- ness, phlegm. Conclusion : Weight of disease mechanism which show satisfaction the conditions of standard of reliability, appear the results of Questionnaires, against previous study investigated reliability of Questionnaires make it through the standard of reliability.

• 동의생리병리학회지
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• 제25권1호
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• pp.1-7
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• 2011

The aim of the present study was to investigate the development of theory of the deficiency of yin-yang in the spleen-stomach(脾胃). The spleen-stomach theory is a very valuable composition of the oriental medicine. Its first theoretical basis was established by Li Dong Yuan(李東垣) who wrote Piweilun("脾胃論"). He insisted the importance of spleen yang(脾陽) which is the "postnatal base of life" by transforming and transporting of food essence and fluids and raises the clear. After him, his theory had influenced many descendant medical men. one of them, Ye Tian Shi(葉天士) found out the differences of between stomach and spleen. Especially he focused on the stomach yin(胃陰) which is easy to be exhausted by dry-fire(燥火). And he also made another remedy, "the stomach yang should be moved well(宣通胃陽)", which emphasize on that cold and stagnant cause the deficiency of the stomach yang. After that, spleen yin(脾陰) theory was set up by Tang Zong Hai(唐宗海). His spleen yin(脾陰) was deeply related with digestive enzymes and pancreas, because his theory was established by comparing oriental and western medicine. These four theories became the theoretical basis of the deficiency of yin-yang in the spleen-stomach(脾胃), and similar symptom of the spleen-stomach(脾胃) could be categorized according to the pattern identification(辨證) which was developed from these four theory.