• Journal of Nutrition and Health
• /
• 제24권3호
• /
• pp.166-178
• /
• 1991

식이 중에 첨가된 conzyme $Q_{10}$이 ADR을 투여한 횐쥐의 체내 지질과산화대사에 미치는 영향을 규명하기 위하여 두가지 실험을 실시하였다. 실험1에서는 basal diet로 적응시킨 후 바로 4주간 실험식이를 공급함과 동시에 ADR을 투여하였으나 실험2에서는 coenzyme $Q_{10}$이 미리 섭취된 상태하에서 지질과산화에 대한 영향을 관찰하고자 basal diet로 적응시킨 다음 4주간 실험식이만을 급여한 후 다시 4주간 실험1과 같은 방법으로 ADR투여와 실험식이 공급을 병행하였다. 실험군은 실험1과 2에서 모두 ADR 2수준 (1.Omg/kg B.W./week. 2.0mg/kg B.W./week)과 coenzyme $Q_{10}$ 3수준(무첨 가군, 0.1g/kg diet 및 0.5g/kg diet)에 의한 6개의 실험군과 basal diet만을 공급하는 대조군을 설정하였다. 본 실험에서 얻어진 결과를 요약하면 혈장과 심장 mitochondria내의 과산화지질 함량은 수준별 ADR 투여에 의해 유의적으로 증가되었고 coenzyme $Q_{10}$ 첨가에 의하여 이러한 경향은 조절되었다. 실험2에 있어서 심장 mitochondria의 과산화지질 함량은 coenzyme $Q_{10}$의 첨가에 의해 실험1에 비하여 그 감소효과가 더욱 증대되었다. 지질과산화 대사에 관여하는 효소 활성도의 변화를 조사하기 위하여 적혈구와 심장조직내에서의 glutathione peroxidase (GSH-Px), superoxide dismutase(SOD)와 catalase 활성도를 측정하였다. 실험1에서 GSH-Px 활성도는 적혈구 내에서는 차이가 없었고 고수준의 ADR을 투여 한 실험군의 심장조직에서만 coenzyme $Q_{10}$에 의해 유의적으로 증가되었으며, SOD 활성도 역시 적혈구에서는 별다른 변화가 관찰되지 않았고 심장조직 내에서 ADR투여로 증가되었으나 coenzyme $Q_{10}$의 급여로 그 활성도가 더욱 유도되었다. Catalase의 활성도는 심장조직 내에서는 변화가 없었고. 적혈구내에서 ADR투여로 그 활성도가 증가되었으나 coenzyme $Q_{10}$의 급여는 그 활성도의 유도효과를 나타내지 못하였다. 실험2의 적혈구내에서는 GSH-Px활성도가 고수준의 ADR의 투여에 의해 유의적으로 감소된 것을 제외하고는 별다른 변화를 나타내지 않았다. 심장조직의 효소활성도에 있어서는 GSH-Px의 경우, 고수준의 coenzyme $Q_{10}$의 급여에 의해 유의적으로 증가 되었으며 SOD활성도는 ADR 투여에 의해 증가되었으나 coenzyme $Q_{10}$의 공급에 의해 더욱 유도되었다. 그러나 catalase 활성도는 모든 처리군들 사이에서 차이를 나타내지 않았다. 이상의 결과로 미루어보아 ADR투여로 유발되는 심장의 손상은 생체내 지질과산화반응의 유도가 중요한 원인이 되고 이는 coenzyme $Q_{10}$ 급여에 의해 유의적인 억제효과를 나타낼 수 있음을 알 수 있다.

• 대한화장품학회지
• /
• 제26권1호
• /
• pp.149-162
• /
• 2000

Coenzyme Q10(CoQ10)은 피부를 포함한 모든 생체조직에 존재하는 널리 알려진 조효소이다. 전자전달에 관여하는 퀴논링은 세포에서 에너지를 생성하기 위한 매우 중요한 기능을 가지고 있다. CoQ10은 피부에서 항산화제로서 연구되어 왔으며, 최근 외용제로써 노화억제와 주름개선작용에 대해 보고된 바 있다. 이런 보고들은CoQ10이 항산화제로서 산화-환원작용을 통해 피부의 방어기능에 중요한 역할을 한다는 점을 시사하며, 일반적으로 산화-환원작용은 피부에서 흑화과정의 조절에도 많은 영향을 미친다. 따라서 본 연구자들은 CoQ10 이 피부의 색소조절기능이 있는지 알아보고자 하였다. 인체 정상 색소세포에CoQ10을 0.05-0.5 mM 처리한 결과 0.5, 0.25mM에서 멜라닌의 생합성을 약 50% 저해하였으며 이는 알려진 미백제인 Kojic acid나 vitamin C와 유사한 수준이었다. 또한, CoQ10은 인체 정상 색소세포에서 자외선이나 세포내 cAMP 증가 유도물질에 의한 멜라닌 생성을 억제하였다. 그러나 tyrosinase inhibitor인 kojic acid와는 달리, in vitro tyrosine hydroxylase의 억제효과는 보이지 않았다. CoQ10을 자외선으로 tanning을 유도시킨 brown guinea pig에 4주간 도포하고 육안 및 chromameter를 이용하여 미백효과를 측정한 결과, vehicle처리군에 비해 미백효과가 있음을 확인할 수 있었다. 이상의 결과에서 coenzyme Q10 은 in vitro및 in vivo에서 미백효과를 지닌 물질임을 확인할 수 있었다.

• Journal of Nutrition and Health
• /
• 제25권6호
• /
• pp.501-510
• /
• 1992

식이중에 첨가된 coenzyme ${Q}_{10}이 ADR을 투여한 흰쥐의 심근미세구조 변화에 미치는 영향을 검토하기 위하여 ADR 2수준(1.0 및 2.0mg/kg B.W/week)과 coenzyne ${Q}_{10} 3수준(0, 0.1및 0.5g/kg diet)에 의한 6개의 실험군과 basal diet만을 공급하는 대조군을 설정하여 8주간 실험동물을 사육하였다. 체중증가량은 ADR의 투여수준이 높을수록 유의적으로 감소되었으나 coenzyme ${Q}_{10}급여에 의한 회복효과는 나타나지 않았다. 전자현미경을 통한 관찰결과, ADR투여는 심근세포내 미세구조의 변성을 유도함이 확인 되었고 특히 mitochondria를 비롯한 세포기관의 소실 및 파괴를 관찰할 수 있었으나 coenzyne ${Q}_{10}을 미리 급여한 군에서는 그정도가 완화되었다.

• 한국치위생학회지
• /
• 제14권5호
• /
• pp.775-781
• /
• 2014

Objectives : The purpose of the study is to investigate the protective effect of coenzyme $Q_{10}$ on cytotoxicity effect of dental monomers in odontoblast(MDPC-23). Methods : MDPC-23 was incubated with the(co)monomers triethylene glycol dimethacrylate (TEGDMA) with and without addition of coenzyme $Q_{10}$. The cell proliferation and survival was determined using WST-1 assay. The level of reactive oxygen species(ROS) was measured by immunofluorescent staining for DCF-DA. Results : TEGDMA treatment decreased the cell proliferation by dose dependently(0.1, 1, 2.5, 5, 10 mM) on the growth of MDPC-23 cells. Coenzyme $Q_{10}$ showed cell proliferation from 5 to $500{\mu}M$ by WST-1 assay. Pre-treatment coenzyme $Q_{10}$ showed the antioxidant effect on proliferation and viability of MDPC-23 after 48h(p<0.05). The positive cells were observed in non-coenyme $Q_{10}$ treatment group(group 2) in comparison with coenyme $Q_{10}$ pre-treatment group(group 1) by DCF-DA. The fluorescence positive cells showed 14.715(group 1) and 19.788(group 2) using image J system. Conclusions : TEGDMA induced cytotoxicity. The MDPC-23 cell death was associated with the increasing ROS. Coenyme $Q_{10}$ showed the antioxidant effects by decreasing ROS. This effects may contribute to the treatment of periodontal disease induced by TEGDMA after operation.

• 한국축산식품학회지
• /
• 제33권1호
• /
• pp.67-74
• /
• 2013

The aims of this research were to produce oil-in-water ${\beta}$-lactoglobulin/alginate (${\beta}$-lg/Al) nanoemulsions loaded with coenzyme $Q_{10}$ and to investigate the combined effects of heating temperature and alginate concentration on the physicochemical properties and encapsulation efficiency of ${\beta}$-lg/Al nanoemulsions. In ${\beta}$-lg/Al nanoemulsions production, various heating temperatures (60, 65, and $70^{\circ}C$) and alginate concentrations (0, 0.01, 0.03, and 0.05%) were used. A transmission electron microscopy was used to observe morphologies of ${\beta}$-lg/Al nanoemulsions. Droplet size and zeta-potential values of ${\beta}$-lg/Al nanoemulsions and encapsulation efficiency of coenzyme $Q_{10}$ were determined by electrophoretic light scattering spectrophotometer and HPLC, respectively. The spherically shaped ${\beta}$-lg/Al nanoemulsions with the size of 169 to 220 nm were successfully formed. The heat treatments from 60 to $70^{\circ}C$ resulted in a significant (p<0.05) increase in droplet size, polydispersity, zeta-potential value of ${\beta}$-lg/Al nanoemulsions, and encapsulation efficiency of coenzyme $Q_{10}$. As alginate concentration was increased from 0 to 0.05%, there was an increase in the polydispersity index of ${\beta}$-lg/Al nanoemulsions and encapsulation efficiency of coenzyme $Q_{10}$. This study demonstrates that heating temperature and alginate concentration had a major impact on the size, polydispersity, zeta-potential value and encapsulation efficiency of coenzyme $Q_{10}$ in ${\beta}$-lg/Al nanoemulsions.

• 운동영양학회지
• /
• 제13권1호
• /
• pp.29-35
• /
• 2009

The purpose of this study was to investigate the effects of oral supplementation of coenzyme Q10 (CoQ10) for 4 weeks on the plasma free oxygen radical and total antioxidant capacity at resting and after one bout exercise in healthy old men. Thirty volunteers with an average (+/-SD) age of 62.59+/-5.3 years participated in this study and were divided with three groups; CoQ10 (200 mg daily) group, vitamin C & E (800mg, 400 IU daily) group, and placebo group. A cycle exercise (60% HRR) test was performed at the end of study. Blood samples were taken for the analyses at rest and pre-, post-, 30min after cycle exercise, before and after the 4 weeks of supplementation. After supplementation, there were no significant differences in the plasma free oxygen radical levels and total antioxidant capacity at resting. Plasma free oxygen radical level and total antioxidant capacity in three groups were significantly elevated after exercise, however, it did not vary significantly between groups. CoQ10 supplementation showed significant difference in total antioxidant capacity during recovery phase compared with placebo group. Our results demonstrated that supplementation of CoQ10 in healthy old men improve blood total antioxidant capacity after one bout exercise, despite no alteration of plasma free oxygen radical levels.

• Journal of Microbiology and Biotechnology
• /
• 제22권2호
• /
• pp.230-233
• /
• 2012

Rhizobium radiobacter T6102 was morphologically purified by the aniline blue agar plates to give two distinct colonies; white smooth mucoid colony (T6102W) and blue rough colony (T6102B). The coenzyme $Q_{10}$ ($CoQ_{10}$) was produced just by T6102W, showing 2.0 mg/g of $CoQ_{10}$ content, whereas the T6102B did not produce the $CoQ_{10}$. All of the used $CoQ_{10}$ biosynthetic precursors enhanced the $CoQ_{10}$ production by T6102W. Specifically, the supplementation of 0.75 mM isopentenyl alcohol improved the $CoQ_{10}$ concentration (19.9 mg/l) and content (2.4 mg/g) by 42% and 40%, respectively.

• Nutrition Research and Practice
• /
• 제4권4호
• /
• pp.311-316
• /
• 2010

The purpose of this study was to investigate the relationship between birth weight and appetite related hormones, insulin resistance, and antioxidant status in overweight children aged 9-10 years. Thirty-four healthy overweight children (18 boys, 16 girls) were evaluated with respect to anthropometric measurement, lipid profiles, leptin, ghrelin, glucose, insulin, C-peptide, lipid soluble vitamins, and antioxidant enzyme activities. I found that birth weight was negatively correlated with insulin resistance parameters, ghrelin, and coenzyme Q10 levels. There was a significant positive correlation between present BMI and leptin level, while a negative correlation was noted between the BMI and $\alpha$-tocopherol and lycopene levels. When total subjects were classified into three groups by tertiles of birth weight, the lowest tertile of birth weight (LTB) group showed higher levels of fasting glucose, HOMA-IR, and ghrelin level than the highest tertile of birth weight (HTB) groups. On the other hand, HTB group showed an increased oxidative stress (decreased coenzyme Q10 level and catalase activity) compared to the LTB group. In conclusion, plasma ghrelin level might play an important role in accelerated growth in overweight children with LTB. Increased insulin resistance is present in overweight children with LTB, while decreased coenzyme Q10 and catalase activity in overweight children with HTB. These results suggest that birth weight might be an important factor for determination of treatment for obesity related complications in childhood obesity.

• Journal of Nutrition and Health
• /
• 제46권3호
• /
• pp.218-225
• /
• 2013

Although the functional ingredient has been evaluated by the Korea Food and Drug Administration (KFDA) based on scientific evidence, the levels of scientific evidence and consistency of the results might vary according to emerging data. Therefore, periodic re-evaluation may be needed for some functional ingredients. In this study, we re-evaluated scientific evidence for the antioxidant activity of coenzyme Q10 as a functional ingredient in health functional food. Literature searches were conducted using the Medline and Cochrane, KISS, and IBIDS databases for the years 1955-2010 with the search term of coenzyme Q10 in combination with antioxidant. The search was limited to human studies published in Korean, English, and Japanese. Using the KFDA's evidence based evaluation system for scientific evaluation of health claims, 33 human studies were identified and reviewed in order to evaluate the strength of the evidence supporting a relation between coenzyme Q10 and antioxidant activity. Among 33 studies, significant effects for antioxidant activities were reported in 22 studies and their daily intake amount was 60 to 300 mg. Based on this systematic review, we concluded that there was possible evidence to support a relation between coenzyme Q10 intake and antioxidant activities. However, because inconsistent results have recently been reported, future studies should be monitored.

• Fisheries and Aquatic Sciences
• /
• 제11권3호
• /
• pp.172-176
• /
• 2008

To develop a potent strain for the production of coenzyme $Q_{10}$, a photosynthetic bacterium was isolated from silt of the Nakdong River in Korea. Using l6S-rDNA sequence analysis, the isolated strain was identified as Rhodobacter sphaeroides. A stable improvement in its $CoQ_{10}$ content was achieved by chemical mutation, upon which the content of $CoQ_{10}$(2.94 mg/g dry cell) was increased by approximately 1.9-fold, comparable to that of R. sphaeroides reported in other studies. The isolate is a potentially valuable microorganism for mass production of $CoQ_{10}$, and may provide an appropriate model for further study of economical mass production.