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http://dx.doi.org/10.13065/jksdh.2014.14.05.775

The protective effect of coenzyme Q10 on cytotoxicity of regin monomer of odontoblast caused by TEGDMA  

Lee, Ahreum (Department of Dental Hygiene, Dongseo University)
Park, Soyeong (Department of Dental Hygiene, Dongseo University)
Lee, Kyung Hee (Department of Dental Hygiene, Dongseo University)
Publication Information
Journal of Korean society of Dental Hygiene / v.14, no.5, 2014 , pp. 775-781 More about this Journal
Abstract
Objectives : The purpose of the study is to investigate the protective effect of coenzyme $Q_{10}$ on cytotoxicity effect of dental monomers in odontoblast(MDPC-23). Methods : MDPC-23 was incubated with the(co)monomers triethylene glycol dimethacrylate (TEGDMA) with and without addition of coenzyme $Q_{10}$. The cell proliferation and survival was determined using WST-1 assay. The level of reactive oxygen species(ROS) was measured by immunofluorescent staining for DCF-DA. Results : TEGDMA treatment decreased the cell proliferation by dose dependently(0.1, 1, 2.5, 5, 10 mM) on the growth of MDPC-23 cells. Coenzyme $Q_{10}$ showed cell proliferation from 5 to $500{\mu}M$ by WST-1 assay. Pre-treatment coenzyme $Q_{10}$ showed the antioxidant effect on proliferation and viability of MDPC-23 after 48h(p<0.05). The positive cells were observed in non-coenyme $Q_{10}$ treatment group(group 2) in comparison with coenyme $Q_{10}$ pre-treatment group(group 1) by DCF-DA. The fluorescence positive cells showed 14.715(group 1) and 19.788(group 2) using image J system. Conclusions : TEGDMA induced cytotoxicity. The MDPC-23 cell death was associated with the increasing ROS. Coenyme $Q_{10}$ showed the antioxidant effects by decreasing ROS. This effects may contribute to the treatment of periodontal disease induced by TEGDMA after operation.
Keywords
antioxidant; cell death; coenzyme $Q_{10}$; odontoblast; triethylene glycol dimethacrylate(TEGDMA);
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