• Biomedical Science Letters
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• v.8 no.3
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• pp.189-193
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• 2002

We have identified and analyzed the 5'-coding region of SCN5A gene in Korean genome. Although its sequence has already been reported in western countries it is still important to confirm our own sequence for the establishment of Korean-suitable diagnosis on genetic basis. Total RNAs were obtained from three healthy Korean adult hearts and reversely transcribed. RT products were then subjected to PCR reaction followed by DNA sequencing. Three different sets of SCN5A primers were designed and used for the amplification of 5'-coding region of SCN5A from Korean genome. Amplified sequence was roughly one-10th of the entire SCN5A mRNA in size and its detailed sequence was completely matched up to the previously reported sequence. There was no difference between three heart samples, either. So, SCN5A was concluded as the relatively stable gene comparing to other genes that are involved in long QT syndrome.

• Korean Journal of Microbiology
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• v.30 no.5
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• pp.397-402
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• 1992

The cDNA of RNA segment coding for VP7 of human rotavirus isolated from patient's stool at Seoul area was synthesized, amplified by polymerase chain reaction, field in with Klenow fragment of DNA polymerase I and cloned into pUC19. The cDNA sequence was determined and compared with that of VP7 coding RNA segments of group A rotaviruses isolates in foreign country. Over 90% sequence homology was found with serotyppe I sepcific WA1 and RE9 strains. Comparative analysis of the deduced amino acid sequences within the two variable regions (amino acid residue 87 through 101 and 208 through 221) with WA1 and RE9 strains also showed high degree of sequence similarity with each other.

• Proceedings of the KOSOMBE Conference
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• v.1997 no.05
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• pp.84-87
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• 1997

In this study, for the coding of stereoscopic video sequence, two approaches are presented based on simulcast mathod and sidefield image format. The field sequential method for stereoscopic visualization have been specified. Also, camera parameter and shooting conditions for each test sequence are studied. Coding method based on sidefield format structure revealed better performance over simulcast in PSNR.

• The Transactions of the Korea Information Processing Society
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• v.7 no.2
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• pp.529-535
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• 2000

In this paper, we propose a new method of designing the vector quantizer which is robustness to coding results and independent of statistical characteristics of an input image in wavelet transformed image coding processes. The most critical drawback of a conventional vector quantizer is the degradation of coding capability resulted from the discordance between quantizer objective image and statistical characteristics of training sequence which is for generating representing vector. In order to resolve the problem of conventional methods, we use independent random-variables and pseudo image to which image correlation and edge component were added, as a training sequence for generating representing vector. We have done a computer simulation in order to compare coding capability between a vector quantizer designed by the proposed method and one with the conventional method using real image as same as that is objective to coding of training sequence used in codebook generation. The results show the superiority of the proposed vector quantizer method at the aspect of coding capability compared to conventional one. They also clarify the problems of conventional methods.

• Journal of Internet Computing and Services
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• v.24 no.1
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• pp.27-37
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• 2023

In this paper, we define a new Lucas-Padoovan sequence using the Padovan sequence, and a new topological interconnection network is constructed using it. Coding nonnegative integers using subsequences of the Lucas-Padovan sequence, and using this to construct a new Lucas-Padovan cubes to deal with topological properties.

• Journal of Microbiology
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• v.37 no.2
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• pp.102-110
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• 1999

Genetic determinant for the secondary metabolism was studied in heterologous expression in Streptomyces lividans TK-24 using Streptomyces griseus ATCC 10137 as a donor strain. Chromosomal DNA of S. griseus was ligated into the high-copy number Streptomyces shuttle plasmid, pWHM3, and introduced into S. lividans TK-24. A plasmid clone with 4.3-kb BamHI DNA of S. griseus (pMJJ201) was isolated by detecting for stimulatory effect on actinorhodin production by visual inspection. The 4.3-kb BamHI DNA was cloned into pWHM3 under the control of the strong constitutive ermEp promoter in both directions (pMJJ202); ermEp promoter-mediated transcription for coding sequence reading right to left: pMJJ203; ermEp promoter-mediated transcription for coding sequence reading left to right) and reintroduced into S. lividans TK-24. The production of actinorhodin was markedly stimulated due to introduction of pMJJ202 on regeneration agar. The introduction of pMJJ202 also stimulated production of actinorhodin and undecylproidigiosin in submerged culture employing the actinorhodin production medium. Introduction of pMJJ203 resulted in a marked decrease of production of the two pigments. Nucleotide sequence analysis of the 4.3-kb region revealed three coding sequences: two coding sequences reading left to right, ORF1 and ORF2, one coding sequence reading right to left, ORF3. Therefore, it was suggested that the ORF3 product was responsible for the stimulation of antibiotic production. The C-terminal region of ORF3 product showed a local alignment with Myb-related transcriptional factors, which implicated that the ORF3 product might be a novel DNA-binding protein related to the regulation of secondary metabolism in Streptomyces.

• Journal of Communications and Networks
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• v.7 no.3
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• pp.243-247
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• 2005

We propose an efficient block least-mean-square (BLMS) adaptive algorithm, in conjunction with error control coding, for direct-sequence code division multiple access (DS-CDMA) systems. The proposed adaptive receiver incorporates decision feedback detection and channel encoding in order to improve the performance of the standard LMS algorithm in convolutionally coded systems. The BLMS algorithm involves two modes of operation: (i) The training mode where an uncoded training sequence is used for initial filter tap-weights adaptation, and (ii) the decision-directed where the filter weights are adapted, using the BLMS algorithm, after decoding/encoding operation. It is shown that the proposed adaptive receiver structure is able to compensate for the signal-to­noise ratio (SNR) loss incurred due to the switching from uncoded training mode to coded decision-directed mode. Our results show that by using the proposed adaptive receiver (with decision feed­back block adaptation) one can achieve a much better performance than both the coded LMS with no decision feedback employed. The convergence behavior of the proposed BLMS receiver is simulated and compared to the standard LMS with and without channel coding. We also examine the steady-state bit-error rate (BER) performance of the proposed adaptive BLMS and standard LMS, both with convolutional coding, where we show that the former is more superior than the latter especially at large SNRs ($SNR\;\geq\;9\;dB$).

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.734-738
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• 2005

A gene coding for xylanase from alkali-tolerant Bacillus alcalophilus AX2000 was cloned into Escherichia coli $DH5\alpha$ using pUC19. Among 2,000 transformants, one transformant showed clear zone on the detection agar plate containing oat-spells xylan. Its recombinant plasmid, named pXTY99, was found to carry 7.0 kb insert DNA fragment. When the nucleotide sequence of the cloned xylanase gene (xynT) was determined, xynT gene was found to consist of 1,020 base-pair open reading frame coding for a poly-peptide of 340 amino acids with a deduced molecular weight of 40 kDa. The coding sequence was preceded by a putative ribosome binding site, and the transcription initiation signals. The deduced amino acid sequence of xylanase is similar to those of the xylanases from Bacillus sp. Nl37 and B. stearothermophilus 21 with $61\%$ and $59\%$ identical residues, respectively.

• Applied Biological Chemistry
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• v.38 no.3
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• pp.224-231
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• 1995

To increase the expression of a foreign protein in transgenic plant, the benefits of 5'-untranslated leader sequences of tobacco mosaic virus (TMV) RNA or soybean glycinin gene, Gy2, fused to a protein coding sequence were exploited. pGA643-derived plasmid contains 355 promoter of cauliflower mosaic virus, protein coding sequence of maize 10 kDa zein (10kZ) and Gy2 terminator. The leader from Gy2 or TMV RNA was inserted between the promoter and the coding sequence in each construct. The recombinant DNAs were introduced into tobacco plants by Agrobacterium mediated leaf disc transformation method. Although the transgene without the leader had more transcripts than the others, mRNAs containing the leader were translated more efficiently. It might be due to difference in the length of 5'-untranslated sequence and context surrounding the AUG codon, but could be sequence specific rather. These results suggest that the leader sequences of Gy2 and TMV play important roles as an enhancer in translational control of foreign gene in transgenic tobacco plant.

• Journal of Broadcast Engineering
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• v.13 no.3
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• pp.289-298
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• 2008

We propose an SNR-scalable coding algorithm for three-dimensional mesh sequences based on singular value decomposition (SVD). SVD achieves a coding gain by representing a mesh sequence with a small number of basis vectors and singular values. First, we introduce a bit plane coding scheme and derive a quantitative relationship between each bit plane and the reconstructed image quality. Using the relationship, we develop a rate-distortion (RD) optimized coding algorithm. Moreover, we propose prediction techniques to exploit the spatio-temporal correlations in real mesh sequences. Simulation results demonstrate that the proposed algorithm provides significantly better RD performance than conventional SVD coders.