• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 추계학술대회
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• pp.33-40
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• 2002

We, Tong Yang Moolsan Co. Ltd. (TYM) set up the mass-production system for virus-free seed garlic via tissue culture technique. TYM's tissue culture technique is called as 'Multiple shoot propagation technique'. This technique can lead mass propagation of genetically homogeneous seed garlic in a short period because of its highly proliferation rate of in vitro shoots ($15^{10}$ /year). TYM researchers applied the technique to some selected garlic cultivars with superior characteristics and carried out field test of productivity in the inside and outside of the country for several years. According to the yearly results of field test with virus-free seed garlic, we ascertained that virus-free seed garlic can produce the highly yield increase (max. above $50\%$) and also can enhance the product quality. Consequently, we estimated that TYM's seed garlic will contribute to farmers with increase of income and can elevate the national position of garlic market in the world for its competitive power of technical and production cost.

• Journal of Animal Science and Technology
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• 제65권2호
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• pp.427-440
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• 2023

The current study evaluated carbon dioxide (CO₂) emissions from beef and pork production and distribution chains in the South Korean meat industry. Data from industrial example farms and slaughterhouses were assessed on the basis of both the guidelines from the United Kingdom's Publicly Available Specification (PAS) 2050:2011 and the Korea Environmental Industry & Technology Institute carbon footprint calculation. The main factors for our estimations were animal feeds, manure waste, transportation, energy and water, refrigerants, and package data. Our analyses show that 16.55 kg CO₂ equivalent (eq) was emitted during the production of 1 kg of live cattle. When retail yields and packing processes were considered, the CO₂-eq of 1 kg of packaged Hanwoo beef was 27.86 kg. As for pigs, emissions from 1 kg of live pigs and packaged pork meat were 2.62 and 12.75 kg CO₂-eq, respectively. While we gathered data from only two farms and slaughterhouses and our findings can therefore not be extrapolated to all meats produced in the South Korean meat industry, they indicate that manure waste is the greatest factor affecting ultimate CO₂ emissions of packaged meats.

• 한국수소및신에너지학회논문집
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• 제35권3호
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• pp.249-256
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• 2024

The cement industry emits a large amount of greenhouse gases compared to other industries, with about 60% of CO₂ emissions from the decarbonation of limestone and about 40% from the combustion of fossil fuels. Therefore, the cement industry needs to reduce greenhouse gases through carbon capture, utilization, and storage technology. Capturing CO₂ and synthesizing it into methanol is feasible and also useful as raw material for the chemical industry and as marine fuel. In this study, We aimed to produce methanol from syngas produced by capturing CO₂ emissions. Process simulations were performed under various conditions such as syngas ratio, temperature, and pressure for the production of synthesis gas and methanol, and the results showed that the optimal amount of methanol production at a synthesis gas ratio of 2.03.

• Journal of Microbiology and Biotechnology
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• 제34권5호
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• pp.1126-1134
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• 2024

The production of disulfide bond-containing recombinant proteins in Escherichia coli has traditionally been done by either refolding from inclusion bodies or by targeting the protein to the periplasm. However, both approaches have limitations. Two broad strategies were developed to allow the production of proteins with disulfide bonds in the cytoplasm of E. coli: i) engineered strains with deletions in the disulfide reduction pathways, e.g. SHuffle, and ii) the co-expression of oxidative folding catalysts, e.g. CyDisCo. However, to our knowledge, the relative effectiveness of these strategies has not been properly evaluated. Here, we systematically compare the purified yields of 14 different proteins of interest (POI) that contain disulfide bonds in their native state when expressed in both systems. We also compared the effects of different background strains, commonly used promoters, and two media types: defined and rich autoinduction. In rich autoinduction media, POI which can be produced in a soluble (non-native) state without a system for disulfide bond formation were produced in higher purified yields from SHuffle, whereas all other proteins were produced in higher purified yields using CyDisCo. In chemically defined media, purified yields were at least 10x higher in all cases using CyDisCo. In addition, the quality of the three POI tested was superior when produced using CyDisCo.

• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.230-233
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• 2012

Rhizobium radiobacter T6102 was morphologically purified by the aniline blue agar plates to give two distinct colonies; white smooth mucoid colony (T6102W) and blue rough colony (T6102B). The coenzyme $Q_{10}$ ($CoQ_{10}$) was produced just by T6102W, showing 2.0 mg/g of $CoQ_{10}$ content, whereas the T6102B did not produce the $CoQ_{10}$. All of the used $CoQ_{10}$ biosynthetic precursors enhanced the $CoQ_{10}$ production by T6102W. Specifically, the supplementation of 0.75 mM isopentenyl alcohol improved the $CoQ_{10}$ concentration (19.9 mg/l) and content (2.4 mg/g) by 42% and 40%, respectively.

• 한국수소및신에너지학회논문집
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• 제35권2호
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• pp.140-145
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• 2024

High temperature co-electrolysis of H₂O-CO₂ mixtures using solid oxide cells has attracted attention as promising CO₂ utilization technology for production of syngas (H₂/CO), feedstock for E-fuel synthesis. For direct supply to E-fuel production such as hydrocarbon and methanol, the outlet gas ratio (H₂/CO/CO₂) of co-electrolysis should be controlled. In this work, current voltage characteristic test and product gas analysis were carried out under various reaction conditions which could attain proper syngas ratio.

• Ocean and Polar Research
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• 제38권4호
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• pp.303-314
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• 2016

We tested the combined effects of increased partial pressure of $CO_2$ ($pCO_2$) and temperature on the reproduction and survival of the copepod Calanus sinicus from Asan Bay, the Yellow Sea under laboratory conditions to understand the impact of acidification on copepods. Egg production rate, survival rate, and fecal pellet production of C. sinicus were not affected by 1305 ppm $pCO_2$ or with combined treatments of temperature and $pCO_2$, including $8^{\circ}C$ and 289 ppm $pCO_2$ (ambient), $8^{\circ}C$ and 753 ppm $pCO_2$ (high $pCO_2$), $12^{\circ}C$ and 289 ppm $pCO_2$ (high temperature), and $12^{\circ}C$ and 753 ppm $pCO_2$ (greenhouse), for 5 or 10 d of exposure. However, egg hatching success of C. sinicus decreased significantly in the greenhouse treatment compared with the ambient or the high $pCO_2$ treatments. These results suggest that a combined treatment ($pCO_2$ and temperature) affected egg viability more than a single treatment($pCO_2$).

• The Korean Journal of Physiology
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• 제4권2호
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• pp.25-31
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• 1970

Tissue homogenates of Ehrlich ascites tumor tissues and several normal tissue of mice were incubated separately in medium maintaining $C^{14}$_acetate concentrations of 5, 10, 20, 30, 40, 50 and 60 mg%, in order to determine maximum oxidative rates of acetate. In every incubation experiments, respiratory $CO_2$ samples rapped by alkaline which was placed in the center well of the incubation blask were analyzed for total $CO_2$ Production rates and their radoactivies. The fractions of $CO_2$ from medium acetate to total $CO_2$ production rate were obtained with relative specific activities (RSA) which were calculated by ratio between specific activities (SA) of $CO_2$ and medium $CO^{14}$_acetate and $CO_2$ production rates from medium acetate were calculated from RSA and total $CO_2$ production rates. Maximum plateau values of oxidative rates described above were determined at incubation experiments of various concentrations of medium acetate and compared the oxidative rates of acetate of tumor with those of normal tissues such as kidney, brain and liver. Maximum plateau values of total $CO_{2}$ Production rates were obtained at acetate concentration of 20 mg% and represent $25.0{\pm}0.54\;{\mu}M/hr/gm$ in the brain, $16.3{\pm}2.5$ in the kidney, $9.1{\pm}1.78$ in the liver and $11.5{\pm}3.2\;{\mu}M/hr/gm$ in the ascites tuners. Substancial $CO_2$ yield was observed in the tumor tissues as in the normal tissues. On the other hand, plateau values of RSA were $25.7{\pm}1.04%$ in thee brain, $9.1{\pm}0.72%$ in the kidney, $2.5{\pm}0.73%$ in the liver and $0.51{\pm}0.12%$ in the tumor tissues. $CO_2$ yields from the medium acetate, were 4.19 in the kidney, 2.28 in the brain, 0.228 in the liter and $0.059\;{\mu}M/hr/gm$ in the tumor tissue. These show wide range even in the normal tissue but remarkable decrease in the tumor tissue. This fact means that further oxidation of acetate was inhibited remarkably in the tumor tissue.

• Preventive Nutrition and Food Science
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• 제22권2호
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• pp.131-137
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• 2017

Maximum production of isoquercetin and quercetin simultaneously from rutin by subcritical water hydrolysis (SWH) was optimized using the response surface methodology. Hydrolysis parameters such as temperature, time, and $CO_2$ pressure were selected as independent variables, and isoquercetin and quercetin yields were selected as dependent variables. The regression models of the yield of isoquercetin and quercetin were valid due to the high F-value and low P-value. Furthermore, the high regression coefficient indicated that the polynomial model equation provides a good approximation of experimental results. In maximum production of isoquercetin from rutin, the hydrolysis temperature was the major factor, and the temperature or time can be lower if the $CO_2$ pressure was increased high enough, thereby preventing the degradation of isoquercetin into quercetin. The yield of quercetin was considerably influenced by temperature instead of time and $CO_2$ pressure. The optimal condition for maximum production of isoquercetin and quercetin simultaneously was temperature of $171.4^{\circ}C$, time of 10.0 min, and $CO_2$ pressure of 11.0 MPa, where the predicted maximum yields of isoquercetin and quercetin were 13.7% and 53.3%, respectively. Hydrolysis temperature, time, and $CO_2$ pressure for maximum production of isoquercetin were lower than those of quercetin. Thermal degradation products such as protocatechuic acid and 2,5-dihydroxyacetophenone were observed due to pyrolysis at high temperature. It was concluded that rutin can be easily converted into isoquercetin and quercetin by SWH under $CO_2$ pressure, and this result can be applied for SWH of rutin-rich foodstuffs.

• 공업화학
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• 제24권6호
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• pp.616-620
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• 2013

본 연구에서는 자원 재활용-에너지에 바탕을 둔 돈분, 계분, 혼합물의 연소특성에 대하여 고찰하였다. 시험편은 건조 오븐을 이용하여 항량까지 건조시킨 후에, 콘칼로리미터(ISO 5660-1)를 이용하여 연소성질을 시험하였다. 그 결과 돈분의 최대유효연소열(78.72 MJ/kg)은 계분(69.41 MJ/kg)과 비교하여 탄화수소의 많은 양 때문에 비교적 증가했다. 또한 돈분의 $CO_2$ 발생속도(0.1959 g/s)와 총연기발생률($419m^2/m^2$)도 각각에 대하여 계분보다 증가하였다. 반면에 계분의 CO 발생량(0.0996 kg/kg)과 CO 발생속도(0.0034 g/s)는 계분이 함유하고 있는 많은 무기물 함량 때문에 각각 돈분보다 높았다. 따라서 높은 연소 에너지는 탄화수소 함량에 의존하여 발생되는 것으로 판단된다.