• Korean Journal of Medicinal Crop Science
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• v.26 no.6
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• pp.447-454
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• 2018

Background: The leaf temperature ($T_{LEAF}$) is one of the most important physical parameters governing water and carbon flux, including evapotranspiration, photosynthesis and respiration. Cnidium officinale is one of the important folk medicines for counteracting a variety of diseases, and is particularly used as a traditional medicinal crop in the treatment of female genital inflammatory diseases. In this study, we developed a model to estimate $T_{Leaf}$ of Cnidium officinale Makino based on black globe temperature ($T_{BGT}$). Methods and Results: This study was performed from April to July 2018 in field characterized by a valley and alluvial fan topography. Databases of $T_{LEAF}$ were curated by infrared thermometry, along with meteorological instruments, including a thermometer, a pyranometer, and an anemometer. Linear regression analysis and Student's t-test were performed to evaluate the performance of the model and significance of the parameters. The correlation coefficient between observed $T_{LEAF}$ and calculated $T_{BGT}$ obtained using an equation, developed to predict $T_{LEAF}$ based on $T_{BGT}$ was very high ($r^2=0.9500$, p < 0.0001). There was a positive relationship between $T_{BGT}$ and solar radiation ($r^2=0.8556$, p < 0.0001), but a negative relationship between $T_{BGT}$ and wind speed ($r^2=0.9707$, p < 0.0001). These results imply that heat exchange in leaves seems to be mainly controlled by solar radiation and wind speed. The correlation coefficient between actual and estimated $T_{BGT}$ was 0.9710 (p < 0.0001). Conclusions: The developed model can be used to accurately estimate the $T_{Leaf}$ of Cnidium officinale Makino and has the potential to become a practical alternative to assessing cold and heat stress.

• Proceedings of the Korean Society of Life Science Conference
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• 2002.09a
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• pp.50-50
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• 2002

• Journal of Korean Ophthalmic Optics Society
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• v.7 no.1
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• pp.39-43
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• 2002

This study was investigated the anti-angiogenic activities of Cnidium officinale Makino and Tabanus bovinus by using Chorioallantoic membrane (CAM) and rat cornea. First experiment, the fertilized chicken eggs were kept in a humidified egg incubator at $37^{\circ}C$. After 4 days incubation, about 3 ml of albumin was aspirated from eggs with an hypodermic needles through a small hole drilled at the narrow end of the eggs, and the shell membrane on the floor of the air sac was peeled away. Embryos with chorioallantois of 3~5 mm in diameter were employed for the assay of antiangiogenic activity. Retinoic acid was used as a positive control for this experiment. After 48 h of treatment. branching pattern of blood vessels below coverslip containing retinoic acid ($10{\mu}g/egg$) was dramatically decreased. A simiar angiogenic inhibition was observed in the CAM treated with $50{\mu}g/egg$ of Cnidium omcinale Makino and Tabanus bovinus extracts. Second experiment, rat corneal neovascularization was induced by suturing (one stitch) the cornea with 10-0 nylon, and terramycin was applied on the cornea for I week to prevent corneal inflammation. In the cornea of rats untreated with herbal extracts, numerous vessels were usually seen invading the cornea by day 2 or 3 after suture, and reaching the lesion area within 5~6 days. Corneal neovascularization was gradually increased and peaked at 3 weeks. In contrast to this, herbal extracts conspicuously inhibited the angiogenesis, Oral administration of herbal extracts (20 mg/kg body weight/day) for 4 weeks significantly inhibited the rat corneal angiogenesis induced by suture, and the length of blood vessels in herbal medicine-treated rat cornea was conspicuously lower than that in control animals. A similar phenomenon was also observed in the rat cornea treated with thalidomide (200 mg/kg body weight/day). These findings indicate the anti-angiogenic properties of Cnidium officinale Makino and Tabanus bovinus, suggesting that these properties may be one of the pharmacological mechanisms underlying the anti-tumor and anti-metastatic activities of herbal extracts tested in this study.

• Korean Journal of Acupuncture
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• v.19 no.1
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• pp.7-13
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• 2002

The effects of Cnidium officinale Makino aqua-acupuncture solution (COMAS) and Cnidium officinale Makino water-extraced solution (COMWS) on the CYP1A1 activity and benzo[a]pyrene(B[a]P)-DNA adduct formation were examined. There were 6.8%, 12.1%, 15.1%, 18.3% and 22.6% inhibition in the activity of cytochrome 4501A1 enzyme with the treatment of $0.1{\times},\;0.5{\times},\;1{\times},\;3{\times},\;and\;5{\times}$ COMAS, respectively. At concentration of $0.1{\times}$ COMAS, the binding of $[^3H]B[a]P$ metabolites to DNA of NCTC-clone 1469 cell was significantly inhibited by 56.9%. These results suggest that COMAS has chemopreventive potential by inhibiting cytochrome P4501A1 activity and benzo[a]pyrene-DNA adduct formation.

• Korean Journal of Medicinal Crop Science
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• v.28 no.6
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• pp.412-420
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• 2020

Background: Measurement of chlorophyll fluorescence (CF) is useful for detection the ability of plants to tolerate environmental stresses such as drought, and excessive sunlight. Cnidium officinale Makino is highly sensitive to water stress and excessive sunlight. In this study, we evaluated the effect of soil water and shade treatment on the photosynthesis and leaf temperature change of C. officinale. Methods and Results: C. officinale was cultivated under uniform irrigation for 1 week drought stress (no watering) for 6 days. A significant decrease in CF was observed on the 5th day of withholding water (approximately 6% of soil water content) regardless of shading. Notably, the Rfd_lss parameter (CF decrease rates) with and without shade treatment was reduced by 73.1% and 56.5% respectively, at 6 days compared with those at the initial stage (0 day). The patterns of the degree of CF parameters corresponded to those of the soil water content and difference between leaf temperature (Ts) and air temperature (Ta). Meanwhile, CF parameters recovered to the 3 - 4 days levels after re-watering, while the soil water potential was completely restored. The suitable soil water content for C. officinale optimal growth was between -5 kPa and -10 kPa in this experiment. Conclusions: Lack of soil water in the cultivation of C. officinale, even with shading, decreased latent heat cooling through transpiration. As a result, heat dissipation declined, and the plant was subjected to drought stress. Soil water content plays a major role in photosynthesis and leaf temperature in C. officinale.

• Korean Journal of Medicinal Crop Science
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• v.27 no.1
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• pp.17-23
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• 2019

Background: Cnidium officinale Makino and Ligusticum chuanxiong Hort. are important medicinal crops in Korea. However, there is insufficient information on the identification of thrips, which attack these plants. Until now, one species of thrips has been recorded as a main pest. Methods and Results: To identify the thrips emerging in C. officinale Makino and L. chuanxiong Hort., these plants were independently cultivated in two local areas. Thirty individuals of each plant species were selected randomly and surveyed for the presence of thrips. After confirming the existence of thrips, 100 thrips individuals were collected from each crop using the beating method. To identify thrips species, we performed PCR-restriction fragment length polymorphism (RFLP)-based analysis using ITS2 primer sets. Six thrips species were identified: western flower thrips (Frankliniella occidentalis), flower thrips (F. intonsa), onion thrips (Thrips tabaci), chrysanthemum thrips (T. nigropilosus), chilli thrips (Scirtothrips dorsalis), and grass thrips (Anaphothrips obscurus). The proportion of these species differed between the host plant species. Conclusions: Six thrips species were major pests of two medicinal crops. Integrated pest management is required to control these thrips species, and will enhance the yield and quality of C. officinale and L. chuanxiong.

• Korean Journal of Acupuncture
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• v.19 no.2
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• pp.57-61
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• 2002

The activities of phase I enzymes in the liver of mice were examined following the intragastric application of Cnidium officinale Makino aqua-acupunture solution (COMAS). Treatment of mice with COMAS resulted in decreases of cytochrome P450 1A1-dependent ethoxyresorufin-O-deethylation and cytochrome P450 2E1-dependent p-nitrophenol and aniline hydroxylation activities. These findings suggest that COMAS has chemopreventive potential by inhibiting cytochrome P450 1A1 and cytochrome P450 2E1 activities in mice.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.405.2-405.2
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• 2002

A near infrared (NIR) method was developed to analyze specious diversity for morphologically similar umbelliferous herbal medicine. Cnidium officinale Makino. This herbal medicine has been widely used as 'chungung' without any discrimination of its quality and original plants. though it has the ambiguous origins of plants between various countries especially Korea. China and Japan. It is named by Cnidium officinale Makino in Korea and Japan. in comparison with Ligusticum chuanxiong Hort. in China. (omitted)

• Weed & Turfgrass Science
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• v.4 no.4
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• pp.279-287
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• 2015

The present research was carried out in order to investigate the occurrence of problematic weed species in Cnidium officinale Makino. Field in South Korea. Total 53 sites of the 3 different regions in S. Korea were investigated from May to October, 2014. In Cnidium officinale fields, the identified weeds were distributed in 35 families and 99 species. Total 5 communities that consist of Commelina communis, Eleocharis kuroguwai, Persicaria vulgaris, Chenopodium album-Acalypha australis, and Galinsoga ciliata dominated the appearance. The weeds occurred in Cnidium officinale fields were divided into three groups in principal component plot analysis (PCA). It was observed that in control weeds plots; 20 plants of Cnidium officinale fresh weight is 739.9 g while the uncontrolled plots have no Cnidium officinale plants. The current investigation could be useful for estimation of future weeds occurrence, weed flora dynamics and establishment of weed control methods in Cnidium officinale fields in Korea.

• Korean Journal of Environmental Agriculture
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• v.39 no.1
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• pp.1-9
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• 2020

BACKGROUND: The aim of this study was to investigate the soil chemical properties and soil bacterial community of the cropping system for Cnidium officinale Makino. METHODS AND RESULTS: The bacterial community was analyzed for the relative abundance and principal coordinated analysis (PCoA analysis) by using by Illumina Miseq sequencing. The correlation analysis between soil chemical properties and soil bacterial community were analyzed by Spearman's rank correlation and DISTLM analysis. Soil bacterial community (phylum and class) showed two distinct clusters consisting of cluster 1 (first cropping) and cluster 2 (continuous cropping) from 2 different cultivation methods of Cnidium officinale Makino. PCoA and DISTLM analyses showed that soil pH and Ca significantly affected soil bacterial community in cultivation area of Cnidium officinale Makino. In addition, Spearman's rank correlation showed significant correlation between relative abundance (Acidobacteria and Actinobacteria) and soil factors (soil pH and Ca). CONCLUSION: The results of this study were considered to be important for determining the correlation between soil properties and soil bacterial community of the cropping method for Cnidium officinale Makino. Furthermore, the results will be helpful to investigate the cause of continuous cropping injury of the Cnidium officinale Makino by examining the changes of soil properties and soil bacterial communities.