Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

The Study of Soil Chemical Properties and Soil Bacterial Communities on the Cultivation Systems of Cnidium officinale Makino

일천궁의 연작재배에 따른 토양 이화학성 및 토양세균군집 연구

  • Kim, Kiyoon (Forest Medicinal Resources Research Center, National Institute of Forest Science, Korea Forest Service) ;
  • Han, Kyeung Min (Forest Medicinal Resources Research Center, National Institute of Forest Science, Korea Forest Service) ;
  • Kim, Hyun-Jun (Forest Medicinal Resources Research Center, National Institute of Forest Science, Korea Forest Service) ;
  • Jeon, Kwon Seok (Forest Medicinal Resources Research Center, National Institute of Forest Science, Korea Forest Service) ;
  • Kim, Chung Woo (Environment-Friendly Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Jung, Chung Ryul (Forest Medicinal Resources Research Center, National Institute of Forest Science, Korea Forest Service)
  • 김기윤 (산림청 국립산림과학원 산림약용자원연구소) ;
  • 한경민 (산림청 국립산림과학원 산림약용자원연구소) ;
  • 김현준 (산림청 국립산림과학원 산림약용자원연구소) ;
  • 전권석 (산림청 국립산림과학원 산림약용자원연구소) ;
  • 김충우 (충청북도농업기술원 친환경연구과) ;
  • 정충렬 (산림청 국립산림과학원 산림약용자원연구소)
  • Received : 2020.01.03
  • Accepted : 2020.01.10
  • Published : 2020.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

BACKGROUND: The aim of this study was to investigate the soil chemical properties and soil bacterial community of the cropping system for Cnidium officinale Makino. METHODS AND RESULTS: The bacterial community was analyzed for the relative abundance and principal coordinated analysis (PCoA analysis) by using by Illumina Miseq sequencing. The correlation analysis between soil chemical properties and soil bacterial community were analyzed by Spearman's rank correlation and DISTLM analysis. Soil bacterial community (phylum and class) showed two distinct clusters consisting of cluster 1 (first cropping) and cluster 2 (continuous cropping) from 2 different cultivation methods of Cnidium officinale Makino. PCoA and DISTLM analyses showed that soil pH and Ca significantly affected soil bacterial community in cultivation area of Cnidium officinale Makino. In addition, Spearman's rank correlation showed significant correlation between relative abundance (Acidobacteria and Actinobacteria) and soil factors (soil pH and Ca). CONCLUSION: The results of this study were considered to be important for determining the correlation between soil properties and soil bacterial community of the cropping method for Cnidium officinale Makino. Furthermore, the results will be helpful to investigate the cause of continuous cropping injury of the Cnidium officinale Makino by examining the changes of soil properties and soil bacterial communities.

본 연구에서는 일천궁의 연작재배에 따른 토양 이화학성 및 토양세균군집을 비교 분석하기 위해 천궁 중에서 재배 빈도수가 높은 일천궁의 초작 및 연작 재배지를 선정하여 토양 이화학성 및 토양세균군집 특성을 분석하고, 토양 이화학성 및 토양세균군집 간의 상관관계를 구명하고자 하였다. 토양 이화학성은 농촌진흥청 토양분석법을 이용하였고, 토양세균군집 분석은 Illumina Miseq sequencing system을 이용하여 상대적 빈도수 및 주좌표 분석을 하였다. 토양 이화학성과 토양세균군집 간의 상관관계는 DISTLM과 Spearman's 상관관계 분석을 이용하였다. 일천궁 재배지의 토양세균군집은 일천의 재배법에 따라 뚜렷하게 2개의 cluster로 군집화를 이루었고, 초작 및 연작 재배지 모두 Proteobacteria와 Alphaproteobacteria가 우점종으로 나타났다. 주좌표 분석과 DISTLM 분석에서는 일천궁 재배지의 토양 pH와 Ca이 토양세균의 군집화에 유의적으로 영향을 주고 있음을 확인하였고, Spearman's 상관관계 분석을 통해 일천궁의 재배법에 따라 유의적인 차이를 보였던 Acinobacteria와 Acidobacteria의 상대적 빈도수는 토양 pH, Ca 함량과 유의적인 상관관계를 보이는 것으로 나타났다. 본 연구는 일천궁의 재배법에 따른 토양 이화학성 및 토양세균군집의 변화와 상관관계를 구명하는데 중요한 자료가 될 것으로 판단된다. 또한, 토양 이화학성과 토양세균군집의 변화에 따른 일천궁 재배지의 연작장해 원인을 구명하는데 있어 도움을 줄 수 있을 것이다. 향후 본 연구를 바탕으로 향후 일천궁의 재배법에 따른 곰팡이(fungi)의 군집과 병원성 미생물군집의 변화를 확인하고 토양 이화학성과의 상관관계를 분석할 수 있다면 일천궁의 연작장해 원인을 명확하게 구명하는데 도움을 줄 수 있을 것으로 사료된다.

Keywords

References

  1. Park YK (1998) The study on antioxidative effects and quality comparison of Ligusticum chuanxiong and Cnidium officinale (1). The Korea Journal of Herbology, 12, 103-114.
  2. Lee HW, Cho HG, Park YK (1999) The study on antioxidative effects and quality comparison of Ligusticum chuanxiong and Cnidium offcinale (II). The Korean Journal of Herbology, 14, 1-6.
  3. Song IG, An BR, Seo BI, Park SJ (2009) Molecular maker to identify and origin of Cnidii Rhizoma from Korea and China. The Korean Journal of Herbology, 24, 1-8.
  4. Lee JH, Choi HS, Chung MS, Lee MS (2002) Volatile flavor components and free radical scavenging activity of Cnidium officinale. Korean Journal of Food Science and Technology, 34, 330-338.
  5. Oh YJ, Seo HR, Choi YM, Jung DS (2010) Evaluation of antioxidant activity of the extracts from the aerial parts of Cnidium officinale Makino. Korean Journal of Medicinal Crop Science, 18, 373-378.
  6. Seo YJ, Nam HH, Jang WC, Kim JS, Lee BY (2018) Effect of meteorological factors on evapotranspiration change of Cnidium officinale Makino. Korean Journal of Agricultural and Forest Meteorology, 20, 366-375. https://doi.org/10.5532/KJAFM.2018.20.4.366
  7. Kim DH, Park JM, Kang SM, Lee SM, Seo CW, Lee IY, Lee IJ (2015) Distribution characteristics of weeds and vegetation types in Cnidium officinale field. Weed & Turfgrass Science, 4, 279-287. https://doi.org/10.5660/WTS.2015.4.4.279
  8. Jung CR, Jeong DH, Park HW, Kim HJ, Jeon KS, Yoon JB (2019) Molecular identification of thrips in two medicinal crops, Cnidium officinale Makino and Ligusticum chuanxiong Hort. Korean Journal of Medicinal Crop Science, 27, 17-23. https://doi.org/10.7783/KJMCS.2019.27.1.17
  9. Kang SW, Yeon BY, Hyeon GS, Bae YS, Lee SW, Seong NS, Kang SW (2007) Changes of soil chemical properties and root injury ratio by progress years of post-harvest in continuous cropping soils of ginseng. Korean Journal of Medicinal Crop Science, 15, 157-161.
  10. Zhang YS, Lee GJ, Joo JH, Lee JT, Ahn JH, Park CS (2007) Effect of winter rye cultivation to improve soil fertility and crop production in alpine upland in Korea. Korean Journal of Environmental Agriculture, 26, 300-305. https://doi.org/10.5338/KJEA.2007.26.4.300
  11. Hwang JM, Park KC, Kim SJ (2010) Contents of soil microbial Phospholipid fatty acids as affected by continuous cropping of pepper under Upland. Korean Journal of Soil Science and Fertilizer, 43, 1012-1017.
  12. Jung YJ, Nou IS, Kang KK (2015) Effects of green manure crops on tomato growth and soil improvement for reduction of continuous cropping injury through crop rotation in greenhouse. Korean Journal of Plant Resource, 28, 263-270. https://doi.org/10.7732/kjpr.2015.28.2.263
  13. Jung YJ, Nou IS, Kim YK, Kang KK (2015) Effect of green manure crops incorporation for reduction of Pythium zingiberum in ginger gontinuous cultivation. Korean Journal of Plant Resource, 28, 271-278. https://doi.org/10.7732/kjpr.2015.28.2.271
  14. Kim SH, Seo DC, Park JH, Lee ST, Lee SW, Kim HC, Cho JS, Heo JS (2013) Effects of green manure crops on growth and yield of carrot for reduction of continuous cropping injury of carrot through crop rotation. Korean Journal of Environmental Agriculture, 32, 279-286. https://doi.org/10.5338/KJEA.2013.32.4.279
  15. Lee SW, Lee SH, Seo MW, Park KH, Jang IB (2018) Effects of irrigation and ginseng root residue on root rot disease of 2-years-old ginseng and soil microbial community in the continuous cropping soil of ginseng. Korean Journal of Medicinal Crop Science, 26, 345-353. https://doi.org/10.7783/KJMCS.2018.26.5.345
  16. Park JH, Seo YJ, Choi SY, Zhang YS, Ha SK, Kim JE (2011) Soil physico-chemical properties and characteristics of microbial distribution in the continuous cropped field with Paeonia lactiflora. Korean Journal of Soil Science and Fertilizer, 44, 841-846. https://doi.org/10.7745/KJSSF.2011.44.5.841
  17. Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH et al. (2009) Introducing mothur: open source, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environmental Microbiology, 75, 7537-7541. https://doi.org/10.1128/AEM.01541-09
  18. Schloss PD (2009) A high-throughput DNA sequence aligner for microbial ecology studies. PLoS One, 4, e8230. https://doi.org/10.1371/journal.pone.0008230
  19. Kim KY, Samaddar S, Chatterjee P, Krishnammorthy R, Jeon SY, Sa TM (2019) Structural and functional responses of microbial community with respect to salinity levels in a coastal reclamation land. Applied Soil Ecology, 137, 96-105. https://doi.org/10.1016/j.apsoil.2019.02.011
  20. Yoon YE, Kim JH, Kim SY, Im JU, Kong MS, Lee YH, Lee YB (2015) Determination of main indicator for the changes of chemical properties in greenhouse soils. Korean Journal of Environmental Agriculture, 34, 355-358. https://doi.org/10.5338/KJEA.2015.34.4.47
  21. Lim SU (2005) The Fertilizer, p. 41. ilsinsa, Seoul, Korea.
  22. Wang Y, Tu C, Cheng L, Li C, Gentry LF, Hoyt GD, Zhang X, Hu S (2011) Long-term impact of farming practices on soil organic carbon and nitrogen pools and microbial biomass and activity. Soil and Tillage Research, 117, 8-16. https://doi.org/10.1016/j.still.2011.08.002
  23. Mo AS. Qiu ZQ, He Q, Wu HY, Zhou X B (2016) Effect of continuous monocropping of tomato on soil microorganism and microbial biomass carbon. Communication in Soil Science and Plant Analysis, 47, 1069-1077. https://doi.org/10.1080/00103624.2016.1165832
  24. Xiong W, Zhao Q, Xue C, Xun W, Zhao J, Wu H, Li R, Shen Q (2016) Comparison of fungal community in black pepper-vanilla and vanilla monoculture systems associated with vanilla Fusarium wilt disease. Frontiers in Microbiology, 7, 117.
  25. Song X, Tao B, Guo J, Li J, Chen G (2018) Changes in the microbial community structure and soil chemical properties of vertisols under different cropping systems in Northern China. Frontiers in Environmental Science, 6, 1-14. https://doi.org/10.3389/fenvs.2018.00001
  26. Li W, Liu Q, Chen P (2018) Effect of long-term continuous cropping of strawberry on soil bacterial community structure and diversity. Journal of Integrative Agriculture, 17, 2570-2582. https://doi.org/10.1016/S2095-3119(18)61944-6
  27. Zhao J, Li S, He X, Liu L, Wang K (2014) The soil biota composition along a progressive succession of secondary vegetation in a karst area. PLoS ONE, 9, e112436. https://doi.org/10.1371/journal.pone.0112436
  28. Wu Y, Zeng J, Zhu Q, Zhang Z, Lin X (2017) pH is the primary determinant of the bacterial community structure in agricultural soils impacted by polycyclic aromatic hydrocarbon pollution. Scientific Reports, 7, 40093. https://doi.org/10.1038/srep40093
  29. Li T, Liu T, Zheng C, Kang C, Yang Z, Yao X, Song F, Zhang R, Wang X et al. (2017) Changes in soil bacterial community structure as a result of incorporation of Brassica plants compared with continuous planting eggplant and chemical disinfection in greenhouses. PLoS ONE, 12, e0173923. https://doi.org/10.1371/journal.pone.0173923
  30. Norris V, Grant S, Freestone P, Canvin J, Sheikh F N, Toth I, Trinei M, Modha K, Norman RI (1996) Calcium signaling in bacteria. Journal of Bacteriology, 178, 3677-3682. https://doi.org/10.1128/JB.178.13.3677-3682.1996
  31. Xue L, Ren H, Li S, Leng X, Yao, X (2017) Soil bacterial community structure and co-occurrence pattern during vegetation restoration in karst rocky desertification area. Frontiers in Microbiology, 8, 2377. https://doi.org/10.3389/fmicb.2017.02377
  32. Ali A, Chani IM, Li Y, Ding H, Meng H, Cheng Z (2019) Hiseq base molecular characterization of soil microbial community, diversity structure, and predictive functional profiling in continuous cucumber planted soil affected by diverse cropping systems in an intensive greenhouse region of Northern China. International Journal of Molecular Sciences, 20, 2619. https://doi.org/10.3390/ijms20112619
  33. Lewin GR, Carlos C, Chevrette MG, Horn HA, McDonald BR, Stankey RJ, Fox BG, Currie CR (2016) Evolution and ecology of Actinobacteria and their bioenergy applications. Annual Review of Microbiology, 70, 235-254. https://doi.org/10.1146/annurev-micro-102215-095748
  34. Kim KY, Um YR, Jeong DH, Kim HJ, Kim MJ, Jeon KS (2019) Study on the correlation between the soil bacterial community and growth characteristics of wild-simulated ginseng (Panax ginseng C.A. Meyer). Korean Journal of Environmental Biology, 37, 380-388. https://doi.org/10.11626/KJEB.2019.37.3.380
  35. Fang BZ, Salam N, Han MX, Jiao JY, Cheng J, Wei DQ, Xiao M, Li WJ (2017) Insights on the effects of heat pretreatment, pH, and calcium salts on isolation of rare Actinobacteria from Karstic caves. Frontiers in Microbiology, 8, 1535. https://doi.org/10.3389/fmicb.2017.01535
  36. Navarrete AA, Venturini AM, Meyer KM, Klein AM, Tiedje JM, Bohannan BJM, Nusslein K, Tsai SM, Rodrigues JLM (2015) Differential response of Acidobacteria subgroups to forest-to-pasture conversion and their biogeographic patterns in the western Brazilian amazon. Frontiers in Microbiology, 6, 1443. https://doi.org/10.3389/fmicb.2015.01443