• 한국HCI학회:학술대회논문집
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• 2007.02a
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• pp.302-307
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• 2007

본 논문은 이미지에서 불필요한 영역을 삭제하고, 그 영역을 배경과 어울리게 채워넣는 이미지 인페인팅 방법을 제안한다. 제안하는 인페인팅 방법은 크게 인페인팅 영역을 채우는 밴드 인페인팅(band in-painting)과 seamless cloning으로 나눌 수 있다. 밴드 인페인팅(band in-painting)은 인페인팅 영역의 경계를 따라서 일정한 두께를 가지는 타겟 밴드(target band)를 정의하고, 인페인팅 영역 밖의 모든 픽셀을 중심으로 하는, 타겟 밴드와 같은 모양과 크기를 가지는 소스 밴드(source band)와 타겟 밴드 차이를 계산하여, 그 값의 차이가 가장 작은 소스밴드 영역의 값을 인페인팅 영역에 복사하는 것이다. Seamless cloning은 인페인팅 영역과 입력 이미지의 경계를 없애는 것이다.

• BMB Reports
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• v.38 no.2
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• pp.191-197
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• 2005

Although the human genome has been nearly completely sequenced, the functions and the roles of the vast majority of the genes, and the influences of single nucleotide polymorphisms (SNPs) in these genes are not entirely known. A modified mutation detection method was developed for large-scale cloning of the possible SNPs between tumor and normal cells for facilitating the identification of genetic factors that associated with cancer formation and progression. The method involves hybridization of restriction enzyme-cut chromosomal DNA, cleavage and modification of the sites of differences by enzymes, and differential cloning of sequence variations with a designed vector. Experimental validations of the presence and location of sequence variations in the isolated clones by PCR and DNA sequencing support the capability of this method in identifying sequence differences between tumor cells and normal cells.

• Microbiology and Biotechnology Letters
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• v.20 no.6
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• pp.619-624
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• 1992

Tn5 lac 삽입으로 채소입고병원균에 길항력이 약화된 T-67 및 고추역병균과 참깨역병균에 길항력이 약화된 T-81의 Tn5 lac 유전자 일부와 오른쪽 말단에 있는 길항관련 유전자의 flanking sequence가 cloning된 pAG67 및 pAG81 clone을 선발하였고, pAG67 및 pAG81 clone된 길항관련 유전자의 flanking sequence를 야생 길항균 Pseudomonas maltophilia B-14의 DNA를 probe로 사용하여 Southern hybridization으로 확인하였으며, 제한효소 지도를 작성하여 8Kb 및 4Kb 크기의 flanking sequence가 cloning되었음을 확인하였다. pAG6 및 pAG81의 flanking sequence를 EcoRi-BglII와 EcoRI-MpaI으로 분리하여 유전자 은행으로부터 길항관련 유전자가 cloning된 cosmid clone 7개주를 선발하였다.

• Journal of Microbiology and Biotechnology
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• v.10 no.2
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• pp.264-266
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• 2000

A new GFP-based T-vector for cloning of PCR products was developed by using a green fluorescent protein (GFP) as a mafker. In order to facilitate the DNA inserts, multiple restriction sites, SP6 and T7 RNA polymerase promoter sites, were introduced close to the PCR DNA insertion site of a pCRGv vector. The XcmI-digested pHNT plasmid can be used to clone a 3' A-overhanged PCR DNA amplified by Taq DNA polymerase. A potential method of easing some difficulties from its use along with its cost savings proveded by this vector are likely to lead to the replacement of other T-vectors for PCR DNA cloning.

• Microbiology and Biotechnology Letters
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• v.15 no.5
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• pp.346-351
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• 1987

A cellulase gene of Clostridium themocellum was transferred to Escherichia coli by molecular cloning with pBR322. The gene was carried in a Hind III digested DNA sequence of about 1.8 kb. This Rind III fragment expressed activities on carboxymethyl cellulose (CMC) and on filter gaper in E. coli. The expression of clostridial cellulase gene in E. coli was studied and compared with the pro-ducts of cellulase genes in C. themocellum.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.43-55
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• 2002

Cloning by nuclear transfer in mammals using somatic cells has enormous potential applications. However, somatic cloning has been inefficient in all species in which NT is successful. High abortion and fetal death rates have been observed. These developmental defects have been attributed to incomplete nuclear reprogramming by the somatic cloning process. In this review, we will discuss studies conducted in our labs to understand the nuclear reprogramming process.

• Mycobiology
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• v.37 no.3
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• pp.240-242
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• 2009

An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion with additional enzymes, and purification with PCR purification kits. Using this procedure, PCR cloning vectors with high ligation efficiencies and low blue or false-positive colonies were obtained.

• Microbiology and Biotechnology Letters
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• v.16 no.2
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• pp.126-130
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• 1988

Promoters of an alkali-tolerant Bacillus sp. isolated from soil have been cloned in Bacillus subtilis using promoter probe vector pPL703. The CAT specific activity of a clone harboring the strongest promoter activity among these transformants was 8.01. This activity was 2.5 times higher than that of Bacillus subtilis harboring expression vector pPL708 and was increased after the end of the logarithmic growth phase. In the 2.8kb of inserted DNA fragment, BamHI and Sal I recognition sites were located.

• Genomics & Informatics
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• v.4 no.2
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• pp.80-86
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• 2006

It is clear that the construction of large insert DNA libraries is important for map-based gene cloning, the assembly of physical maps, and simple screening for specific genomic sequences. The bacterial artificial chromosome (BAC) system is likely to be an important tool for map-based cloning of genes since BAC libraries can be constructed simply and analyzed more efficiently than yeast artificial chromosome (YAC) libraries. BACs have significantly expanded the size of fragments from eukaryotic genomes that can be cloned in Escherichia coli as plasmid molecules. To facilitate the isolation of molecular-biologically important genes in Ashbya gossypii, we constructed Ashbya chromosome-specific BAC libraries using pBeloBAC11 and pBACwich vectors with an average insert size of 100 kb, which is equivalent to 19.8X genomic coverage. pBACwich was developed to streamline map-based cloning by providing a tool to integrate large DNA fragments into specific sites in chromosomes. These chromosome-specific libraries have provided a useful tool for the further characterization of the Ashbya genome including positional cloning and genome sequencing.

• Journal of Embryo Transfer
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• v.9 no.1
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• pp.31-41
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• 1994

핵 이식 기술을 이용한 cloning 송아지 생산이 처음 보고(Prather et al., 1987) 된 후, 소 수정란 Cloning에 대한 많은 연구가 분자 생물학 등 여러 분야에서 꾸준히 계속되고 있다. 이 기술은 빈우의 번식 능력을 향상시켜 유전적 개량량을 증대할 수 있는 번식과 육종을 위한 도구로써 많은 잠재력을 지니고 있다. 최근 핵 이식 기술을 이용하여 유전적으로 우수한 빈우로부터 수천개의 수정란을 생산하여, 이들 수정란에게 생산된 송아지가 번식 축군으로 공시되어 있으므로, 그 결과가 주목되나 아직까지는 비용이 많이 들고 송아지 생산 효율이 저조하므로, 가까운 장래에 일반 양축가에 이용될 가능성이 낮다. 그러나 이 기술의 실용화를 위하여 선결되어야 할 많은 문제점들 중, 지난 몇 년 동안 많은 연구기관에서 수행된 활발한 연구의 결실로써, 난포란 제핵, cell fusion 과 oocyte activation의 방법등 주요 장애 요인들이 점차 극복되면서 실용화를 위한 접근이 예견되어지며, 구미의 일부 개량 기관에서는 이를 상업화 하기 위한 여건을 다지고 있다. 그러므로 이 Review에서는 fllicular dynamics, 난포란의 성숙, cell cycle, 난포란 제핵, cell fusion과 oocyte activation, 이식후 핵의 remodeling과 reprogramming에 대한 현재까지의 보고된 자료를 기초로 그 기본 원리를 재고하는데 초점을 맞추었다.