• Korea-Australia Rheology Journal
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• 제21권3호
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• pp.155-160
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• 2009

Micropore filtration of dilute red blood cell (RBC) suspensions is a widely known method for determining red blood cell deformability. Use of this method for cells from various laboratory animal species does require considering the effects of the cell size to pore size ratio and of suspension hematocrit. In general, previous animal studies have utilized 5% hematocrit suspensions and five micron pores, and thus conditions similar to human clinical laboratory practice. However, when used for repeated sampling from small laboratory animals or for parallel multiple samples from different sites in large laboratory animals, the volume of blood sampled and hence the hematocrit of the test suspension may be limited. Our results indicate that hematocrit levels yielding stable values of RBC pore transit time are pore size and species specific: three micron pores = $2{\sim}5%$ for dog and $3{\sim}5%$ for rat; five micron pores $3{\sim}5%$ for dog and $1{\sim}5%$ for rat. An analytical approach using a common expression for calculating transit time is useful for determining the sensitivity of this time to hematocrit alterations and hence to indicate hematocrit levels that may be problematic.

• Archives of Plastic Surgery
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• 제33권1호
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• pp.61-66
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• 2006

The purpose of this study is to introduce the method of palate repair that combines minimal hard palate dissection and radical retropositiong of levator musculature, which was presented by Sommerlad. As this method presents, additional use of the operating microscope enables atraumatic and radical dissection, and it might provide more improved speech function to the patients. A total of 17 patients with cleft palate underwent Sommerlad's method from December 2003 to August 2004. The mean follow-up period was 4.5 months. The use of a microscope provided high quality variable magnification and good illumination at the operating field. Repair was carried out through incisions at the margins of cleft with mucoperiosteal flap elevation. Muscles were rearranged and repaired properly. It was unable to evaluate the improvement of speech because the patients were too young to learn meaningful speech. Average operating time including anesthetic induction time, V-tube insertion and recovery from anesthesia was 2 hours 45 minutes which was not quite different from conventional method's operating time. Oronasal fistula developed in 2 patients of them. One of them was healed spontaneously. As meticulous and radical muscle dissection was possible with Sommerlad's method, we could minimize the trauma to the muscular and neurovascluar structure. In addition, we expect better faculty of speech as a result of this method although longer follow-up time was unavailable.

• 대한수의학회지
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• 제39권4호
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• pp.730-737
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• 1999

A national animal health monitoring system(NAHMS) in Gyeongnam area was started from 1997 to develop statistically valid data for use in estimating disease frequencies in dairy cattle, and the associated costs. The objectives of this study were to : (1) discribe what was done to implement and maintain the system in Gyeongnam ; (2) present selected disease frequencies ; (3) discuss the epidemiological consideration of what was done and implications for the results obtained. Veterinary Medical Officers(VMOs-professors and graduate students from Gyeongsang National University, Faculty of Gyeongnam Livestock Promotion Institute, and Clinic veterinarians) served as data collectors. After training on current disease and management problems of dairy cattle, interview techniques, sampling methods, and data collection instruments, the VMOs participated in selection of the sample herds and data gathering. Forty of 167 dairy herds were selected randomly and the VMOs visited farms once a month for 12 months to collect data about management, disease, inventory, production, preventive treatment, financial and any other relevant data. Strict data quality control devices were used. Specific feed-back was developed for the producers and data collectors. Of the three age groups studied, cows had the greatest number of disease problems. The six disorders found most frequently were (from the highest to the lowest) breeding problems, clinical mastitis, birth problems, gastrointestinal problems. metabolic problems, and lameness. In young stock, respiratory, multiple system, breeding problems, and gastrointestinal problems were pre dominant, whereas in calves, gastrointestinal, respiratory, and integumental problems were major.

• 동의생리병리학회지
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• 제22권1호
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• pp.96-99
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• 2008

Toxoplasma gondiiis a widespread apicomplexan parasite which is able to infect virtually all warm-blooded vertebrates. Twenty-two percent of the U.S. population is infected, but severe disease in adults is mainly limited to immunosuppressed patients. In patients with acquired immunodeficiency syndrome(AIDS), T. gondii causes a life-threatening opportunistic infection, with Toxoplasma encephalitis as its most severe manifestations. T. gondii is also known to cause congenital infection and is among the pathogens with the highest incidence of complications in pregnancies. Despite its clinical importance, only very few therapeutic drugs against T. gondii are available, all of which target the rapidly dividing tachyzoites, leaving the dormant encysted bradyzoite stage unaffected. We searched 15 traditional medicines that have anti-inflammatory effect from dongyibogam and Traditional Chinese medicine. In vitro studies were performed with HeLa cell cultures, with quantification of Toxoplasma growth by a cell proliferation assay. The result of experiment shows the selectivity of Citrus unshiu Markovich is 6.0. This is higher than sulfadiazine (selectivity was 1.63). For in vivo studies, mice were acutely infected intraperitoneally with $10^5$ tachyzoites of the virulent RH strain and then treated per orally for 4 days from 6 hours postinfection. Efficacy was assessed by sequential determination of parasite burdens in peritoneal cavity. In vivo, Citrus unshiu Markoviche inhibited Toxoplasma growth at a concentration of 10㎎/㎏ of body weight per day, the inhibition ratio was estimated to be 64.01%.

• 미생물학회지
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• 제52권3호
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• pp.327-335
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• 2016

본 연구에서는 신규 Amycolatopsis 균주 KCTC 29142를 분리하여 형태학적 관찰, 계통분석 및 화학분류학적 분석 등 다상 분류분석을 통해 분석하였다. KCTC 29142 균주의 에틸아세테이트추출물은 강한 항균활성을 나타났고, 활성물질은 철 이온 킬레이트 물질인 siderochelin A로 동정되었다. 본 연구에서 분리된 siderochelin A는 다제내성균인 Acinetobacter baumanii, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), 및 Escherichia coli (E. coli)에 대해 강한 활성을 보였고, 임상에서 분리된 다제내성균에 대한 MIC를 결정하였다.

• The Journal of Advanced Prosthodontics
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• 제6권6호
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• pp.483-490
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• 2014

PURPOSE. The purpose of this study was to determine the average facial proportions and mandibular movement capacity of 316 first-year dental students who carefully recorded them on each other. MATERIALS AND METHODS. This early exacting clinical experience was closely supervised by the authors in Columbus, Ohio during 1969-70. Five vertical and six horizontal distances were measured on each subject's face. An ala-tragus line and an occlusal line were drawn on the left side of the face to determine if these two lines were parallel. Measurements of mandibular movements involved maximum normal and hinge opening at the incisors and maximum amounts of right, left lateral and protrusive excursions of the mandible. RESULTS. The ala width and distance between the tips of upper right and left canine cusps averaged (35.2 mm and 34.8 mm) but with very large individual variations. The distance between ala to occlusal plane lines was 29.9 mm at the tragus and 31.3 mm near the ala. The angle between orbitale and ala-tragus averaged 13.6 degrees. CONCLUSION. The upper lip length was the most variable and the distance between the pupils was the most stable of the eleven facial measurements. The ala-tragus line and the occlusal plane lines were for all practical purposes parallel. Maximum jaw opening averaged 51.2 mm which was 3.0 times larger than maximal hinge opening of 17.2 mm. The maximum right plus left side jaw excursions (9.2 and 9.4 mm) totaled 18.6 mm, 2.3 times more than the 8.0 mm mean maximum forward protrusion.

• The Journal of Advanced Prosthodontics
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• 제8권6호
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• pp.457-464
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• 2016

PURPOSE. The aim of this study was to determine whether two methods of documentation, print and electronic forms, for the assessment of patient-reported outcomes (PRO) in complete denture wearers provide comparable results. The study also quantified the time needed for filling the forms by each method. MATERIALS AND METHODS. Thirty participants enrolled in a university clinic answered two forms (a questionnaire for denture satisfaction and OHIP-EDENT). They provided answers with two application methods in a random order, with a one-month interval between them: (1) electronic forms on a tablet computer; and (2) print forms. The methods were compared in terms of mean results, correlation/agreement, internal consistency, and spent time. RESULTS. Mean results for both methods were similar for each denture satisfaction item (100-mm VAS) and OHIP-EDENT summary score. Both questionnaires presented good internal consistency regardless of the application method (Cronbach's ${\alpha}=0.86$ or higher). Correlation and agreement between the methods regarding specific items was at least moderate for the majority of cases. Mean time for the electronic and print forms were 9.2 and 8.5 minutes, respectively (paired t test, P=.06, non-significant). CONCLUSION. The electronic method is comparable to print forms for the assessment of important PRO of prosthetic treatment for edentulism, considering the results and time needed. Findings suggest the viability of replacing print forms with a tablet for applying the tested inventories in clinical trials.

• Journal of Microbiology and Biotechnology
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• 제28권8호
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• pp.1247-1259
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• 2018

Raspberries are polyphenol-rich fruits with the potential to reduce the severity of the clinical signs associated with obesity, a phenomenon that may be related to changes in the gut microbiota. The aim of this study was to investigate the effect of raspberry supplementation on the fecal microbiota using an in vivo model of obesity. Obese diabetic db/db mice were used in this study and assigned to two experimental groups (with and without raspberry supplementation). Fecal samples were collected at the end of the supplementation period (8 weeks) and used for bacterial 16S rRNA gene profiling using a MiSeq instrument (Illumina). QIIME 1.8 was used to analyze the 16S data. Raspberry supplementation was associated with an increased abundance of Lachnospiraceae (p = 0.009), a very important group for gut health, and decreased abundances of Lactobacillus, Odoribacter, and the fiber degrader S24-7 family as well as unknown groups of Bacteroidales and Enterobacteriaceae (p < 0.05). These changes were enough to clearly differentiate bacterial communities accordingly to treatment, based on the analysis of UniFrac distance metrics. However, a predictive approach of functional profiles showed no difference between the treatment groups. Fecal metabolomic analysis provided critical information regarding the raspberry-supplemented group, whose relatively higher phytosterol concentrations may be relevant for the host health, considering the proven health benefits of these phytochemicals. Further studies are needed to investigate whether the observed differences in microbial communities (e.g., Lachnospiraceae) or metabolites relate to clinically significant differences that can prompt the use of raspberry extracts to help patients with obesity.

• 한국지구과학회지
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• 제24권3호
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• pp.117-127
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• 2003

본 질적 연구에서는 사범대학에서의 예비교사 교육과 현직 교사 재교육의 개선방안에 대한 현장 교사들의 의견을 수렴하였다. 현장에서 좋은 수업을 실천하고 있는 사례로 선정된 10명의 과학 교사들과의 심층면담을 통하여 그들이 진단하는 교사양성 교육과 재교육 실태 및 개선방안을 분석하였다. 사범대학에서의 예비교사 교육과 관련하여 좋은 수업을 하는 현장의 과학교사들은 (1) 사범대학이 교사양성 기관으로서의 정체성을 확보해야 하며, (2) 이론과 실제가 조화된 교육과정을 통하여 현장과의 연계성을 고려한 교사양성 교육을 실시해야 하고, 이를 위하여 (3) 사범대학 교수진에 교과교육 전공자를 확보해야 한다고 주장하였다. 또한, 교사들은 현직 교사 연수와 관련하여 (1) 현직 교사들 스스로 계속교육에 대한 인식을 전환하여 교사로서의 전문성 개발을 위하여 끊임없이 노력하여야 하며, (2)교사 연수 내용과 방법의 현장성을 높여서 교사들이 필요로 하는 교사연수로 바뀌어야 하며, (3)교직 사회에 진정한 의미의 수업장학 풍토의 정착이 필요하다고 강조하였다. 성공적인 교실개혁을 위한 교사 (재)교육의 필요성도 논의하였다.

• 대한간호학회지
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• 제37권5호
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• pp.801-809
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• 2007

Purpose: This study was aimed at exploring the current status of graduate programs for an advanced practice nurse(APN) to recommend future directions of APN education. Methods: A total of 142 students enrolled in seven APN specialty programs, 67 professors who were involved in APN education, and nine nurse administrators participated in the study. Data was collected by questionnaires and focus group interviews. Results: The current definition of APN was found not to be specific enough to represent expected roles of APN in regards to knowledge, attitudes, roles, and skills. Standard curricula employed regardless of the area of APN specialty, lack of qualified clinical practice settings, as well as prepared instructors were found to be problematic. Conclusion: The following needs to be addressed: 1. redefining of APN roles, 2. tailoring specialty areas of APN, 3. consolidating educational programs, and 4. ensuring APN role models and faculty. Suggesting a CNS role in Korean APN, areas of APN should be rearranged toclarify their roles and educational programs need to be further developed to meet the expectations and quality of APNs. It is necessary to ensure APN's employment in the health care system by laws and policies to perform advanced nursing roles.