• Archives of Pharmacal Research
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• 제17권1호
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• pp.5-10
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• 1994

From the methanolic extract of aerial parts of Clematis koreana var. umbrosa, one new triterpenoidal saponin, 3-O-${\beta}$-D-xylopyranosyl(1-3)-${\alpha}$-L-arabinopyranosyl oleanolic acid 28-O-${\alpha}$-L-rhamnopyranosyl(1-4)-${\beta}$-glucopyranosyl(1-6)-${\beta}$-glucopyranosyl ESTER, along with five known aromatic compounds and two known triterpenoidal saponins were isolated.

• 한국약용작물학회지
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• 제14권5호
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• pp.299-302
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• 2006

Three known triterpenoids were isolated from MeOH extract of C. apiifolia (Ranunculaceae). Their structures were identified as oleanolic acid (1), ursolic acid (2), hederagenic acid (3) by comparison of their physicochemical and spectral data with the literature values. Among them, 2 was isolated for the first time from this plant. The isolated compounds were evaluated for their cytotoxicity against L1210, HL-60, SK-OV-3 tumor cell lines. All compounds 1-3 were shown good activities with $IC_{50}$ values ranging from 7.7 to $25.6\;{\mu}g/ml. This result suggests that triterpenoids 1-3 are main cytotoxic principles of this plant.

• 대한임상검사과학회지
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• 제53권2호
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• pp.165-173
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• 2021

할미밀망은 국내에서 자생하는 미나리아재비과의 낙엽활엽덩굴식물이다. 어린 잎은 식용으로 사용되며 줄기와 뿌리는 약재로 사용된다. 할미밀망 줄기에 대한 항산화 연구는 보고되었으나 잎에 대한 연구는 현재 수행되지 않았다. 본 연구에서는 할미밀망을 70% 에탄올 추출물로 제조하고 항산화 및 항염증 생리 활성을 비교하였다. 항산화 활성을 측정하기 위해 5가지 분석(총 폴리 페놀 함량, 플라보노이드 함량, 환원력, ABTS 및 DPPH 라디칼 소거 활성)이 사용되었으며 모든 분석에서 농도 의존적 효과를 나타냈다. 항염증 활성을 조사하기 위해 RAW 264.7 세포를 사용했다. 할미밀망은 농도(31.25-250 ㎍/mL)에서 세포 독성을 나타내지 않았다. 할미밀망(250 ㎍/mL)은 LPS에 비해 세포의 수지상적 형태 변화를 억제하였고(34.4%), NO (77.4%), IL-6 (85.5%) 및 TNF-α (41.2%)의 발현을 억제함으로써 염증을 낮추는 효과를 나타내었다. 또한 할미밀망(250 ㎍/mL)은 LPS 단독 처리군에 비해 COX-2 (79.8%), iNOS2 (93.9%), IL-6 (87.6%) 및 TNF-α (77.3%)의 유전자 발현을 억제했다. 이러한 결과는 할미밀망이 우수한 항산화 및 항염증 작용을 가지고 있음을 보여준다. 그러므로 할미밀망은 향후 항산화 및 항염증 효과를 가진 천연 생물 유래 소재로 활용될 수 있을 것이다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.26-26
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• 2020

Daphne L. (Thymelaceae) comprises about 95 species distributing worldwide from N Africa, N India, SE Asia to E Asia and the coast of the Mediterranean of Europe. In Korea, five species of this genus have been described. In this study, we included four species (D. genkwa, D. pseudomezereum, D. kiusiana, D. jejudoensis) from Korea, excluding cultivated D. odora. The morphological characters through local surveys and the re-classification of the specimens collected in the Korea National Herbarium (KH) were carried out and distribution maps for each taxon were also prepared. The major characters include habit, trichomes in winter bud, leaf, and twig, phyllotaxis, inflorescence, size of calyx lobe and trichomes in the calyx tube, etc. The distribution map showed that D. genkwa is mainly distributed in the coastal area of Hwanghaenam-do, Pyeongannam-do, Jeollabuk-do and Jeollanam-do, whereas D. pseudomezereum is distributed in the limestone zone of Gangwon-do, Jeollabuk-do, and Gyeongsangbuk-do. Similarly, D. kiusiana is mostly found in Jeollanam-do, Gyeongsangnam-do, and Jeju-do. In addition, D. jejudoensis is known to be distributed in forests of Murueng, Andeok, and Seonheul-ri in Jeju-do, but recently, new habitat is discovered in the island forest areas of Jeollanam-do. However, some of these individuals showed the characteristics of D. kiusiana, thus before come to any conclusion detailed taxonomic review of D. jejudoensis and D. kiusiana is required.

• 한국환경복원기술학회지
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• 제14권5호
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• pp.17-35
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• 2011

The purpose of this study is to offer the raw data for management and conservation of national park by systematic and objective surveying and analysing the flora distributed in the Hwarang district, Gyeongju national park. The results are as follows. The vascular plants in this site were summarized as 396 taxa: 95 families, 272 genera, 351 species, 2 subspecies, 38 varieties and 5 forma. The rare plants designated by Korea Forest Service were 5 taxa: Exochorda serratifolia S.Moore, Potentilla discolor Bunge, Prunus yedoensis Matsum, Lysimachia coreana Nakai and Inula salicina var. asiatica Kitam.. The korean endemic plants were 6 taxa: Populus tomentiglandulosa T.B.Lee, Clematis trichotoma Nakai, Lespedeza maritima Nakai, Lysimachia coreana Nakai, Forsythia koreana (Rehder) Nakai and Weigela subsessilis (Nakai) L.H.Bailey. The specific plants by floristic region were 30 taxa: Asplenium sarelii Hk., Salix chaenomeloides Kimura, Lysimachia barystachys Bunge, Achillea alpina L., Celtis aurantiaca Nakai, Vitex negundo var. incisa (Lam.) C.B.Clarke and so forth. The plants with approval for delivering oversea were 6 taxa: Clematis trichotoma Nakai, Exochorda serratifolia S.Moore, Glycine soja Siebold & Zucc., Lespedeza maritima Nakai, Lysimachia coreana Nakai and Weigela subsessilis (Nakai) L.H.Bailey. The naturalized plants were 49 taxa: Bilderdykia dumetora (L.) Holub, Descurainia pinnata Britton, Oxalis corymbosa DC., Bidens frondosa L., Panicum dichotomiflorum Michx. and so forth. The invasive alien plants were 2 taxa: Ambrosia artemisiifolia L. and Aster pilosus Willd. In future, if we will survey the flora of whole Gyeongju national park, we will offer the help to establishing the conservation plan of ecosystem in Gyeongju national park.

• Journal of Species Research
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• 제2권2호
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• pp.191-202
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• 2013

The article provides information on type specimens of 150 taxa of vascular plants from Korea, kept in the Herbarium of the Komarov Botanical Institute of the Russian Academy of Sciences. For all specimens, type category is indicated. Lectotypes for the names of Clematis brachyura Maxim., C. spectabilis Palib., Corydalis wilfordii Regel, Poa viridula Palib., Polygonum marretii H. L$\acute{e}$v., P. sagittatum L. var. hallaisanense H. L$\acute{e}$v., P. taquetii H. L$\acute{e}$v. and P. thunbergii Sieb. et Zucc. var. coreana H. L$\acute{e}$v. were designated. Type specimens examined in this article belong to the taxa described by Russian botanists V.L. Komarov, K.J. Maximovicz and I.V. Palibin, French botanist A.A.H. L$\acute{e}$veill$\acute{e}$ (some with co-author E. Vaniot) and others.

• 생물환경조절학회지
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• 제23권4호
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• pp.288-292
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• 2014

클레마티스의 경삽에 의한 삽목번식이 잘 되지 않는 7-8월의 영양번식방법으로 수삽의 가능성을 알아보기 위해 실험을 수행하였다. 수삽시 적정한 삽수길이 및 발근제 처리효과에 대해 알아보기 위해 클레마티스 '호노라'의 1마디 삽수와 2마디 삽수를 NAA $0.1mg{\cdot}L^{-1}$에 처리하였다. NAA $0.1mg{\cdot}L^{-1}$ 처리구는 삽수길이에 관계없이 100% 발근되었지만, 무처리구는 1마디, 2마디 삽수 각각 약 30%와 19%로 발근율이 현저히 낮았다. 뿌리수, 뿌리길이도 NAA $0.1mg{\cdot}L^{-1}$ 처리구가 우수하였고, 그 효과는 1마디 삽수에서 더 크게 나타났다. '호노라' 수삽의 결과를 바탕으로 2013년 클레마티스 '파이루'의 1마디 삽수를 이용하여 NAA(0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$) 처리농도에 따른 발근율을 비교 하였다. 발근소요일수는 약 70-74일로 약 10주가 소요되었고 농도에 따른 유의한 차이는 없었다. 하지만 발근율은 NAA 처리농도가 높을수록 높았으며, NAA $1.0mg{\cdot}L^{-1}$ 처리시 약 90% 발근되었다. 전반적인 뿌리의 생육은 NAA $0.5mg{\cdot}L^{-1}$ 이상의 농도에서 향상되었다. 하지만 '파이루'는 NAA를 처리하지 않아도 76%이상 발근되는 것으로 보아 '호노라'보다 수삽에 의한 번식이 용이한 품종으로 생각된다.

• 미생물학회지
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• 제5권1호
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• pp.24-33
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• 1967

Fifty-two specimens of the infected plants were collected from areas through the country during the year of 1965. We report here 14 unrecorded specoes which were identified in this work. The results are as follows: 1. Erysiphe cichoracearum DC. (Host: Artemisia princeps PAMPAN. var. orien-talls HARA.) 2. Erysiphe cichoracearum DC. (Host: Plantago sp.) 3. Erysiphe cichoracearum DC. (Host: Sonchus oleraceus L.) 4. Erysiphe pisi DE CANDOLLE. (Host: Lespedeza sp.) 5. Erysiphe polygoni DE CANDOLLE. (Host: Clematis patens MORR et DECAIS.) 6. Microsphaera alphitoides GRIFFITHS et MAUBLANCE (Uncinula bifurcata GERARD.) (Host: Quercus serrate GERARD) 7. Microsphaera baumleri MAGNUS. (Host: Robinia pseud-acacia L.) 8. Microsphaera coryli HOMMA. (Host: Corylus heterophylla FISCH. var. japonica KOIDZUMI) 9. Podosphaera tridactyla (WALLROTH) DE BARY. (Host: Prunus leveilleana KOEHN. var. typica NAKAI) 10. Sphaerotheca fuliginea (SCHLECHTENDAHL) POLLACI. (Host: Impatiens balsamina L.) 11. Sphaerotheca fuliginea (SCHLECHTENDAHL) POLLACI. (Host: Zinnia elegans JACQ) 12. Uncinula aceris SACCARDO (Host: Acer negundo L.) 13. Uncinula fraxini MIYABE(U. salmon SYDOW) (Host: Fraxinus rhynchophyllus HANCE.) 14. Uncinula salicis (DE CANDOLLE) WINTER. (Host: Salix gracilistyla MIQ.)

• 농약과학회지
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• 제8권3호
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• pp.220-230
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• 2004

본 연구의 목적은 내의 자생식물 중 살포활성이 있는 식물 종을 선발하는데 있다. 국내의 자생식물 55과 98종 시료로터 MeOH 조추출물을 얻은 다음 24-well plate에서 유채(Brassica napus L.)에 대한 살초검성을 수행하였다. 본 연구에 사용된 98종 중 놀은 살포활성($GR_{50}$ 값 < 1,000 ${\mu}g\;g^{-1}$)을 나타낸 식물은 가중나무(Ailanthus altissima (MILL.) SWINGLE), 고비(Osmunda japonica THUNB.), 더위지기(Artemisia iwayomogi KITAMURA), 두릅(Aralia elata SEEM.), 떡쑥(Gnaphalium affine D. DON), 미국산사나무(Crataegus scabrida SARG.), 사군자(Quisqualis indica L.), 센달나무(Machilus japonica S. et Z.), 소귀나무(Myrica rubra S. et Z.), 실육카(Yucca smalliana FERN.), 양버즘나무(Platanus occidentalis L.), 영춘화(Jasminum nudiflorum LINDL.), 유채(Brassica campestris subsp. napus var. nippo-oleifera MAKINO), 음나무(Kalopanax pictus (THUNB.) NAKAI), 자리공(Phytolacca esculenta V. Houtte), 장딸기(Rubus hirsutus THUNB.), 젓나무(Abies holophylla MAXIM.), 족도리풀(Asarum sieboldii MIQ.), 참으아리(Clematis terniflora DC.), 캐모마일(Anthemis nobilis L.) 등 20종으로 나타났다. 자생식물 98종 중 50종 식물은 중정도의 살초활성을 나타내었으나(1,000 ${\mu}g\;g^{-1}$ < $GR_{50}$ 값 < 2,000 ${\mu}g\;g^{-1}$), 28종 식물은 살초활성을 전혀 나타내지 않았다.

• 대한본초학회지
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• 제38권5호
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• pp.39-47
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• 2023

Objectives : The objective of present study was to investigate the vasorelaxant effects of 20 Korean native plants on isolated rat thoracic aorta precontracted with phenylephrine (PE). Methods : Dried 20 plant materials were extracted 3 times with water, ethanol, or methanol for 3h in the reflux apparatus at 70 ± 5℃. Male SD rats were anesthetized by ether inhalation, and their aorta rings were isolated and placed in 10 ㎖ Krebs Henseleit (KH) buffer. While using an isolated organ-chamber technique, the aorta rings were maintained by bubbling with a gas mixture of 95% O₂-5% CO₂ at 37℃. Changes in isometric tension of aorta rings were recorded via isometric transducers connected to a Powerlab Data Acquisition System. Results : Among the 20 native plants, Chrysanthemum indicum L. flower, Nelumbo nucifera Gaertn. rhizome, Schisandra chinensis (Turcz.) Baill. fruit, Anthriscus sylvestris (L.) Hoffm. root, Corydalis turtschaninovii Besser tuber, Corydalis decumbens (Thunb.) Pers. tuber, and Dolichos lablab L. seed showed significant vasorelaxant effect on the contraction of aorta rings induced by PE. In contrast, Mertensia maritima subsp. asiatica Takeda whole plant, Ajuga decumbens Thunb. whole plant, Trigonotis peduncularis (Trevis.) Benth. ex Baker & S.Moore whole plant, Dioscorea quinquelobate Thunb. rhizome, Allium microdictyon Prokh aerial part, Momordica charantia L. fruit, Carthamus tinctorius L. flower, and Clematis terniflora DC. root constricted more the aorta rings precontracted by PE Conclusion : These results suggest that the possibility as useful herbal resources for the development of functional foods or medicines for hypertension treatment.