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http://dx.doi.org/10.15324/kjcls.2021.53.2.165

Antioxidant and Anti-Inflammatory Activities of Ethanol Extract of Clematis trichotoma Nakai  

Jung, Jaemee (Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University)
Shin, Mijoon (Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University)
Jeong, Naeun (Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University)
Hwang, Dahyun (Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.53, no.2, 2021 , pp. 165-173 More about this Journal
Abstract
Clematis trichotoma Nakai (CTN) is a broad-leaved vine plant belonging to the family Ranunculus, native to Korea. Young leaves are used as food, and the stem and roots are used as medicinal materials. Antioxidant studies have been reported on the stems of CTN, but no studies have been conducted on the leaves. In this study, a 70% ethanol extract of CTN was prepared and its antioxidant and anti-inflammatory activities were investigated. For measuring the antioxidant activity, five assays (polyphenol and flavonoid content, reducing power, 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity) were performed and CTN showed a concentration-dependent effect in all assays. To investigate the anti-inflammatory activity, we used RAW 264.7 cells. The concentrations (from 31.25 to 250 ㎍/mL) of CTN did not show cytotoxicity. CTN (250 ㎍/mL) inhibited dendritic transformation (34.4%) and also inhibited inflammation as seen by reduced levels of NO (77.4%), IL-6 (85.5%) and TNF-α (41.2%) compared to lipopolysaccharide (LPS). CTN (250 ㎍/mL) also suppressed the expression of the following genes: COX-2 (79.8%), iNOS2 (93.9%), IL-6 (87.6%), and TNF-α (77.3%) compared to LPS. These results demonstrated that CTN has excellent antioxidant and anti-inflammatory activities and can therefore be used as a natural biological resource.
Keywords
Anti-inflammation; Antioxidant; Clematis trichotoma Nakai; Functional foods;
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