• Journal of Applied Biological Chemistry
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• 제60권2호
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• pp.113-117
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• 2017

생물학적정화는 미생물을 이용하여 중금속을 포함한 오염물질을 정화하는 방법이다. 효모는 다양한 중금속을 흡착하여 정화하는 능력이 우수하다고 잘 알려졌다. 따라서 본 연구는 여러 중금속 중 하나인 아연에 저항성인 큰 효모를 인위적인 돌연변이를 통해서 얻는 것에 초점을 두었다. 대조구인 효모를 1 mM 농도의 아연이 포함된 배지에서 키운 후 점차 아연의 농도를 높여 최종적으로 80 mM이 되는 지점까지 키웠으며 이 농도는 효모가 점진적 적응을 거쳤어도 성장이 불가능한 한계점 근처이며 이렇게 유도 분리된 효모를 ZnR이라 명명하였다. ZnR은 대조구 효모보다 아연에 대한 저항성이 현저히 증가되었으며 이 외에 카드뮴과 니켈에 대한 저항성도 증가되었지만 구리에 대한 저항성은 오히려 감소 되었다. ZnR이 보여준 아연에 대한 저항성 증가는 유전자 돌연변이에 의한 영구적인 획득 결과이다. 단 기간 내에 아연에 대한 저항성 큰 효모의 인위적인 유도 및 분리는 생물학적정화 연구에 유용하게 쓰여질 정보이다.

• 해양환경안전학회:학술대회논문집
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• 해양환경안전학회 2006년도 추계학술발표회
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• pp.163-168
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• 2006

동해의 해양 심층수 자원 기반 환경 연구의 일부로 청결기술을 적용하여 해수 중의 용존성 미량금속 (Cd, Co. Cu, Ni. Ph. Zn) 농도 분포와 변화를 조사하였다. 강원과 경북 지역에 총 6개 정점을 선정하고, 각 지역마다 최대 채수 수심 각각 200-300m 와 500m 까지 시료를 채취하였다. 본 연구의 미량금속을 위한 연안해수 표준물질 (CASS-4) 의 분석 결과 각 원소 평균 회수율은 Co의 89.4% 에서 Cd 의 99.8%의 범위였다. 용존 미량원소의 분포는 해역 및 시기적인 변화가 다양하게 나타났다 Cd, Ni, Zn의 경우는 영양염 의존형으로 표층에서 낮고 저층으로 갈수록 증가하는 경향이 뚜렷하였다. 그러나 Co, Cu, Ph 은 수직적으로 불규칙적인 분포를 나타내었다. 본 연구의 통해 심층수 원수의 중금속 농도는 해역별 수질 기준과 먹는 물 기준을 만족하였다.

• Journal of Microbiology and Biotechnology
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• 제25권3호
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• pp.381-385
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• 2015

In the present study, drop-on-demand two-dimensional patterning of unstained and stained bacterial cells on untreated clean wafers was newly conducted using an electrospray pulsed jet. We produced various spotted patterns of the cells on a silicon wafer by varying the experimental conditions, such as the frequency, flow rate, and translational speed of the electrospray system in a two-dimensional manner. Specifically, the electrospray's pulsed jet of cell solutions produced alphabetical patterns consisting of spots with a diameter of approximately $10{\mu}m$, each of which contained a single or a small number of viable bacteria. We tested the viability of the patterned cells using two visualization methods. This pattering technique is newly tested here and it has the potential to be applied in a variety of cell biology experiments.

• Ocean and Polar Research
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• 제36권4호
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• pp.319-326
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• 2014

Mineral dust in the polar snow plays an important role both in the climate system of the Earth and in global biogeochemical cycles. Analysis of the concentration and the particle size distribution of mineral dust has been carried out in the snow from the Antarctic surface snow and the Greenland snowpit. Among the various particle size determination techniques, a Multisizer 3 Coulter Counter in a class 100 clean bench counted all particles between 1.1 and $30.0{\mu}m$ with a $50{\mu}m$ aperture tube. The aperture tube size, the concentration of electrolytes and the accuracy of the particle size distribution were determined in this study. The number concentrations from the Antarctic surface snow were 81,843 particles $mL^{-1}$, but those from the Greenland snowpit were 10,666 particles $mL^{-1}$. In the volume distribution, the distributions of mineral dust in both the Antarctic surface snow and the Greenland snowpit showed lognormal distribution in the size interval 1.1 to $6.0{\mu}m$ with the mode, 3.562 and $3.836{\mu}m$, respectively. The analysis technique using a coulter counter for mineral dust could be used for reconstructing paleoclimates from polar ice cores.

• 한국태양에너지학회:학술대회논문집
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• 한국태양에너지학회 2009년도 춘계학술발표대회 논문집
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• pp.139-144
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• 2009

The generation of small hydropower as compared to other different developed environmental methods produces one of the clean energies. In such manner, various application system development through IT technique is being developed for an advanced small hydropower energy resources data mining. However, existing data analysis of New & Renewable Information System for small hydropower resources application is incomplete therefore it limits expressing these information on the Web. Thus for positive usage of small hydropower resources, a more systematic and precise analysis system should be built. This study seeks to develop a map of the domestic small hydropower resources problems to further improve small hydropower resources, developed through Package Tool which can accurately evaluate a wide range of small hydropower basin in a short period of time. Small hydropower Package Tool was calculated using existing Analysis System small hydropower resources which did not provide diverse capabilities resulting to 840 standard basin classified by A and facility capacity, etc., and to assume a 40% annual capacity, expected annual electricity production was calculated. Small hydropower for the national water system of small hydropower resources potential calculated in terms of resources for the development of small hydropower will be utilized as basic data.

• 한국진공학회:학술대회논문집
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• 한국진공학회 1996년도 제11회 학술발표회 논문개요집
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• pp.40-41
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• 1996

Nitridation technique has been receiving much attention for the formation of a thin nitrided buffer layer on which high quality nitride films can be formedl. Particularly, gallium nitride (GaN) has been considered as a promising material for blue-and ultraviolet-emitting devices. It can also be used for in situ formed and stable passivation layers for selective growth of $GaAs_2$. In this work, formation of a thin nitrided layer is investigated. Nitrogen electron cyclotron resonance(ECR)-plasma is employed for the formation of thin nitrided layer. The plasma source used in this work is a compact ECR plasma gun3 which is specifically designed to enhance control, and to provide in-situ monitoring of plasma parameters during plasma-assisted processing. Microwave power of 100-200 W was used to excite the plasma which was emitted from an orifice of 25 rnm in diameter. The substrate were positioned 15 em away from the orifice of plasma source. Prior to nitridation is performed, the surface of n-type (001)GaAs was exposed to hydrogen plasma for 20 min at $300{\;}^{\circ}C$ in order to eliminate a native oxide formed on GaAs surface. Change from ring to streak in RHEED pattern can be obtained through the irradiation of hydrogen plasma, indicating a clean surface. Nitridation was carried out for 5-40 min at $RT-600{\;}^{\circ}C$ in a ECR plasma-assisted molecular beam epitaxy system. Typical chamber pressure was $7.5{\times}lO^{-4}$ Torr during the nitridations at $N_2$ flow rate of 10 seem.(omitted)mitted)

• 대한전자공학회논문지SP
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• 제43권5호
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• pp.52-61
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• 2006

영상 및 신호 처리 분야에 일반적으로 사용되는 직교 웨이블릿 변환은 천이에 대한 민감성과 방향성에 대한 선택도가 떨어지기 때문에 성능에 한계를 가지고 있다. 이러한 단점을 극복하기 위해 복소수 웨이블릿 변환이 사용되고 있다. 본 논문에서는 이중 트리 복소수 웨이블릿과 베르누이-가우스 사전 확률분포를 이용한 효과적인 영상 잡음 제거 방법을 제안하고자 한다. 베르누이-가우스 모델에 대한 파라미터를 추정하기 위해 본 논문에서는 두 가지의 간단하고 반복적이지 않은 방법을 제안한다. 베르누이 랜덤 변수로 표현되는 혼합 파라미터를 추정하기 위해서는 가설-검증 기법을 사용한다. 추정된 혼합 파라미터를 이용하여 신호의 분산은 MGML(maximum generalized marginal likelihood) 추정기를 통하여 추정된다. 복소수 웨이블릿 변환을 사용하여 제안 방법과 알려진 잡음 제거 기법과 비교 실험을 수행하였다. 실험결과를 통해 제안 방법이 적은 계산량으로 고주파 성분이 많은 영상에 대하여 우수한 잡음 제거 결과를 나타냄을 알 수 있다.

• 대한전자공학회논문지SP
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• 제47권1호
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• pp.175-182
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• 2010

본 논문에서는 배경 잡음 환경에서 배경 잡음에 의해 저하된 음성 신호의 명료도를 soft decision 기반의 음성 부재 확률을 이용하여 음성 강화 이득을 수정함으로써 음성의 명료도를 보다 향상시키는 기법을 제안한다. 배경 잡음 환경에서 저하된 음성의 명료도를 향상시키기 위한 기존의 음성 강화 기법으로써 soft decision을 이용하여 오염된 음성 신호로부터 깨끗한 음성 신호만 증폭시키는 알고리즘이 제안되었다. 기존의 음성 강화 기법 보다 음성 구간과 비음성 구간 및 전이 구간에서 강인한 음성 강화 이득을 추정하기 위하여 soft decision 기반의 음성 부재 확률 (Speech Absence Probability)을 음성 강화 이득에 통합한 음성 강화 이득 수정 알고리즘을 제안한다. 제안된 음성 강화 기법의 성능은 다양한 배경 잡음 환경에서 ITU-T P.800의 주관적인 음질 측정 방법인 (Comparison Category Rating) 테스트에 의해서 평가되었으며, 기존의 음성 강화 기법과 비교하여 향상된 성능을 보여주었다.

• Progress in Superconductivity
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• 제7권1호
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• pp.41-45
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• 2005

The diagnostic management of patients with chest pain remains a clinical challenge. Magnetocardiography (MCG) has been proposed as a new non-invasive method for detection of myocardial ischemia. To date, however, MCG technique is not intensively introduced for clinical use. One of the main reasons might be the absence of statistically valid and diagnostically clean criteria, which can determine the presence of certain heart disease. In this work, we suggested a new method to classify the diagnostic value of MCG for the detection of coronary artery disease (CAD) in patients with chest pain. MCG was recorded for three groups (healthy subjects and patients without and with CAD) by means of the 64 channel SQUID gradiometer system installed at a hospital. Using four parameters, which were found to be significantly different between groups, we evaluated a probability, in which parameters can be classified into each group based on the distribution function of the parameter in each group. For all parameters, sum of probabilities was compared between groups to determine the presence of CAD. Our classification method shows that the MCG can be a useful tool to predict the presence of CAD with sensitivity and specificity of higher than $80\%$ each.

• 복식
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• 제62권1호
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• pp.90-103
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• 2012

Felt, is one of the Korean traditional woolen fabrics, that is thermally excellent and durable, so it has been widely used for various living goods from the ancient time to the modern society. Felt makes various changes in colors and shapes, does not change easily even after a long-term use, and is easy to clean. In this study, the origin and history of felt were researched. Also, different kinds and shapes of felt and the currently used manufacturing technique, and how the Korean traditional pattern-applied cultural products were developed using felt were examined as well. The method of this study were as follows. First, felt manufacturing techniques used after investigating the precedent studies, books, records and literatures of museums, the existing relics of felt were examined. Second, the conditions of the traditional pattern-applied cultural products were found out through the precedent studies after examination of kinds and symbols of the Korean traditional patterns. Third, patterns that were applied to cultural products were selected and designed with new patterns. Fourth, the past techniques were conducted with the following three methods by referring to the range of cultural products manufacturing; )color felt made with one color, pattern felt, and pattern-embroidered felt. Fifth, the following products combined with decoration and practical application by applying felt techniques were used. This study was aiming at the development of practical cultural products made with the traditional wool, felt, which can receive a good response from all age groups. It is allegedly required to develop various cultural products through studies and the rediscovery of the national traditional materials. In the future, it is necessary to study in such a way that diversification and differentiation are made through approaches and attempts with various techniques.