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http://dx.doi.org/10.4014/jmb.1401.01012

Viable Bacterial Cell Patterning Using a Pulsed Jet Electrospray System  

Chong, Eui-seok (Aerosol and bioengineering laboratory, Department of Mechanical Engineering, Konkuk University)
Hwang, Gi Byung (Aerosol and bioengineering laboratory, Department of Mechanical Engineering, Konkuk University)
Kim, Kyoungtae (Aerosol and bioengineering laboratory, Department of Mechanical Engineering, Konkuk University)
Lee, Im-Soon (Department of Biological Sciences, Konkuk University)
Han, Song Hee (Department of Biological Sciences, Konkuk University)
Kim, Hyung Joo (Department of Microbial Engineering, Konkuk University)
Jung, Heehoon (Department of Microbial Engineering, Konkuk University)
Kim, Sung-Jin (Aerosol and bioengineering laboratory, Department of Mechanical Engineering, Konkuk University)
Jung, Hyo Il (Department of Mechanical Engineering, Yonsei University)
Lee, Byung Uk (Aerosol and bioengineering laboratory, Department of Mechanical Engineering, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.3, 2015 , pp. 381-385 More about this Journal
Abstract
In the present study, drop-on-demand two-dimensional patterning of unstained and stained bacterial cells on untreated clean wafers was newly conducted using an electrospray pulsed jet. We produced various spotted patterns of the cells on a silicon wafer by varying the experimental conditions, such as the frequency, flow rate, and translational speed of the electrospray system in a two-dimensional manner. Specifically, the electrospray's pulsed jet of cell solutions produced alphabetical patterns consisting of spots with a diameter of approximately $10{\mu}m$, each of which contained a single or a small number of viable bacteria. We tested the viability of the patterned cells using two visualization methods. This pattering technique is newly tested here and it has the potential to be applied in a variety of cell biology experiments.
Keywords
Electrospray; two-dimensional pattern; bacteria treatment methods; pulsed jet; microorganism arrays; bioaerosols;
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