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http://dx.doi.org/10.3839/jabc.2017.019

Characterization of artificially induced zinc-tolerant yeast mutants  

Lee, Sangman (Division of Applied Biology and Chemistry, School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
Publication Information
Journal of Applied Biological Chemistry / v.60, no.2, 2017 , pp. 113-117 More about this Journal
Abstract
Bioremediation is a technique using microorganisms to clean up contaminated pollutants including heavy metals. It is well known that yeasts have a high capacity to remove a wide range of metals by biosorption. Therefore, this study was focused on to obtain yeast mutant that has strong tolerance to zinc (Zn), one of representative heavy metals. The Zn resistant yeast mutant (ZnR) was induced and isolated by growing yeast cells in media containing 1 mM $ZnCl_2$ and gradually increasing the concentration until 80 mM $ZnCl_2$, in which cells were adapted and survived. The induced ZnR cells showed strong tolerance to Zn stress compared with control cells. Moreover, the ZnR cells showed increased tolerance to cadmium and nickel stress but decreased tolerance to copper stress. The increased tolerance of ZnR cells to Zn stress was due to mutation of genes. This study can be useful in bioremediation of heavy metals as the metal tolerant microorganism was artificially induced in short time.
Keywords
Bioremediation; Heavy metal; Mutation; Yeast; Zinc;
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