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Breeding of Phalaenopsis 'SM 333' with Mini Multiple Flower Formation (소형 다화 분지성 호접란 'SM 333' 육성)

  • Park, No Eun;Son, Beung Gu;Kim, Hong Yul;Lim, Ki-Byung
    • Horticultural Science & Technology
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    • v.33 no.1
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    • pp.149-154
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    • 2015
  • A new Phalaenopsis cultivar 'SM 333' was bred by Sangmiwon Orchid, Korea, which produces young plants through tissue culture techniques. The new cultivar 'SM 333', showing the phenotype of multiflora with pink color and small, multibranching-type characteristics, was derived from crossing between Phalaenopsis 'Odoriko' and 'Be Tris'. An elite individual number '02-03-33' later termed 'SM 333' was selected among about 300 individual progenies, based on an intensive selection process covering vegetative and flowering distinctiveness over more than 2 years. In year 2004-2005, the 1st and 2nd characteristic analyses were carried out through performance and uniformity tests. 'SM 333' shows flower color that is bright clean pink (RHS # RP69D) and flower shape that is formal type with 5.0 and 5.8 cm in flower height and width, respectively. 'SM 333' is regarded as raceme flower type suitable for the small casual flower market. The leaves of 'SM 333' grow horizontally and about 20.8 cm in length and 6.5 cm in width. This cultivar also possesses no genetic variation, and is amenable to fast in vitro propagation and easy growth due to its vigorous growth habit. This 'SM333' was registered (Reg. # 2916) with Korea Seed & Variety Service (KSVS) on 1st December, 2009, and the plant breeder's right is currently controlled by Sangmiwon Orchid Company, Korea.

Determination of Nitrovin in Fishery Products by Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS를 이용한 수산물 중 니트로빈의 정량분석법 개발 및 검증)

  • Kim, Joohye;Shin, Dasom;Kang, Hui-Seung;Jeong, Jiyoon;Rhee, Gyu-Seek
    • Journal of Food Hygiene and Safety
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    • v.33 no.2
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    • pp.118-123
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    • 2018
  • The objective of this study was to develop a sensitive method for the identification and determination of nitrovin in fishery products by using a solid-phase extraction (SPE), as performed with a liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted with a mixture of acetonitrile and water, and were then defatted with acetonitrile saturated hexane, after which further clean-up was accomplished with SPE on the hydrophilic-lipophilic balance (HLB) cartridges. The analytes were subsequently ionized in the positive mode of an electrospray ionization (ESI), and where thereby detected in a process of multiple reaction monitoring (MRM). The linearity (expressed as correlation coefficients) of the matrix calibration curves was > 0.985. The limit of the quantification for the nitrovin was measured at 0.001 mg/kg. The accuracy (expressed as average recovery) was noted between 72.1 and 122%. The precision (expressed as coefficient variation) was noted from 2.9 to 16.9%. According to the CODEX CAC/GL-71 guideline accuracy, precision, linearity, and limit of detection were determined in three matrices (which were flatfish, eel and shrimp). The proposed method was suitable for analyzing the associated nitrovin residues. This application and result can also be a factor to contribute to the non-detection drugs management in fishery products.

Heavy Metals in Sediments and Organisms from Tidal Flats along the Mokpo Coastal Area (목포연안 갯벌 및 서식생물에서의 중금속 함량)

  • 나춘기
    • Economic and Environmental Geology
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    • v.37 no.3
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    • pp.335-345
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    • 2004
  • Total and extractable contents of heavy metals were measured in sediment and seafood from Bukhang and Boggil-ri tidal flats along the Mokpo coastal area, south-western part of Korean peninsular in order to assess the degree of metal pollution, metal bioavailability and metal hioaccumulation. The metal concentrations, except Pb were found to be greater than the background concentrations of sediments indicating the progress of cumulative contamination by anthropogenic origin of metals. The order of extractable metal concentrations in sediments were Mn(32-53 mg/kg)>Zn(14-42 mg/tg)>Cu(2.5-17.0 mg/kg)>Pb(2.4-6.8 mg/kg)>Cd(0.5-0.7 mg/kg). However, the amount of metals associated with extractable fraction of sediments were significantly high in Bukhang relative to Boggil-ri. Significant bioaccumulation of all metals, except Pb were observed in seaweed and benthos. The order of bioaccumulation of metals were: in concentration; all biota commonly, Mn(129-374 mg/kg)>Zn(19-106 mg/kg)>Cu(6-87 mg/kg)>Cd(4.6-7.6 mg/kg)>Pb(0.2-3.7 mg/kg), in BCF; Enteromorpha, Cd>Mn>Cu>Zn>Pb, Ilyoplax deschampsi, Cu>Cd>Mn>Zn>Pb, Urechis unicinctus, Cd>Zn>Mn>Cu>Pb. Some metal concentrations of Enteromorpha, especially Cu, Zn, weakly Pb in bukhang, Mn, Cd in boggili-ri, were correlate well with concentrations in sediment, indicating relatively more contaminated by the anthropogenic origin of metals in each tidal flat. The results clearly indicate that the seafood of bukhang, even of Boggili-ri known as clean area, are contaminated with metals.

Prediction and Experiment of Pressure Drop of R22 and R134a on Design Conditions of Condenser (응축기의 설계조건에서 R22와 R134a의 압력강하 예측 및 실험)

  • Kang, Shin-Hyung;Byun, Ju-Suk;Kim, Chang-Duk
    • Journal of Energy Engineering
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    • v.15 no.4 s.48
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    • pp.243-249
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    • 2006
  • An experimental study on the refrigerant-side pressure drop of slit fin an tube heat exchanger has been carried out. A comparison was made between the predictions of previously proposed empirical correlations and experimental data for the pressure drop on design conditions of condenser in micro-fin tube for R22 and Rl34a. Experiments were carried out under the conditions of inlet refrigerant temperature of $60^{\circ}C$ and mass fluxes varying from $150\;to\;250\;kg/m^{2}s$ for R22 and Rl34a. The inlet air conditions are dry bulb temperature of $35^{\circ}C$, relative humidity of 40% and air velocity varying from 0.68 to 1.43 m/s. Experiments show that pressure drop for R134a was $22{\sim}22.6%$ higher than R22 for the degree of subcooling $5^{\circ}C$ For the mass fluxes of $200{\sim}250\;kg/m^{2}s$, the deviation between the experimental and predicted values for the pressure drop was less than ${\pm}20%$ for R22 and Rl34a.

EFFECTS OF ACID TREATMENT OF FLUORIDE APPLIED DENTIN SURFACE ON DENTIN BONDING (불소도포한 상아질면의 산처리가 상아질접착에 미치는 영향)

  • Hwang, Hea-Kyung;Ahn, Sik-Hwan;Kim, Sung-Kyo;Jo, Kwang-Hun;Park, Jin-Hoon
    • Restorative Dentistry and Endodontics
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    • v.21 no.2
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    • pp.602-618
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    • 1996
  • The purpose of this study was to investigate the effect of acid treatment of fluoride applied dentin surface with various concentrations of phosphoric acid for various periods of time on dentin bonding. Dentin specimens prepared from freshly extracted bovine mandibular anterior teeth were divided into fluoridated and nonfluoridated groups. Specimens of nonfluoridated group were pretreated with 10% phosphoric acid for 15 seconds. Those of fluoridated groups were treated with 2% sodium fluoride or 2% stannous fluoride solution for 5 minutes and stored in $37^{\circ}C$ distilled water for 3 days, followed by phosphoric acid treatment. The concentrations of phosphoric acid were 10%, 32% or 50% and the treatment periods of time were 15, 30 or 60 seconds. All the specimens were bonded with All Bond$^{(R)}$ 2 and Bisfil$^{TM}$ composite resin. After bonded specimens were stored in $37^{\circ}C$ distilled water for 24 hours, tensile bond strengths of each specimens were measured and the pretreated dentin and the fractured dentin surfaces were examined under the scanning electron microscope. The results were as follows : The tensile bond strengths from the fluoridated groups were significantly lower than those from the nonfluoridated group when the concentrations of phosphoric acid and the treatment periods of time were equal in all the groups (p<0.05). In general, the higher the concentration of phosphoric acid and the longer the treatment period of time for acid etching on the fluoride applied dentin surface, the higher were the bond strength values. Recovery of bond strength of the dentin bonding agent was better in the NaF applied group than in the $SnF_2$ applied one. SEM findings of NaF applied and $SnF_2$ applied dentin surfaces demonstrated reaction product-covered and partially or completely obstructed dentinal tubules. SEM findings of dentin surfaces fluoridated for 5 minutes followed by etching showed wider tubular openings and more clean dentin surfaces when dentin was etched with higher concentration of phosphoric acid for longer period of time. On the SEM observations of the fractured dentin-resin interface, the etched specimens of fluoridated group showed an adhesive failure mode when the concentration of phosphoric acid and the treatment period of time were same as in the nonfluoridated group. As the concentration of phosphoric acid and the treatment period of time increase during acid etching, the cohesive failure area increased. However, excessive acid etching caused adhesive failure.

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Weed Population Dynamic in Orchard and Their Control by Sequential Application of Oxyfluorfen (과수원(果樹園)의 잡초발생(雜草發生) 동태(動態)와 Oxyfluorfen의 처리시기별(処理時期別) 방제효과(防除效果))

  • Kim, Kil Ung;Kwon, Soon Tae;Choi, Dae Ung;Lee, Sang Back;Kim, Dong Kyun
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.22-26
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    • 1986
  • This study was conducted to investigate weed population dynamic in orchard and to establish an appropriate application time for oxyfluorfen. 28 weed species including Persicaria hydropiper was observed to be problem weeds in orchard. P. hydropiper was on single dominant species which increased importance value of 13.7 on May 15 to 60.79 on August 10, followed by Calystegia hederacea, Alopecurus aequalis, Capsella bursa-pastoris, Roripa islandica, Echinochloa crus-galli, Potulaca olearcea, Elusin indica. Simpson's index increase from 0.11 on May 1 to 0.40 of August 10, indicating the dominance of specific weed species. The effective herbicide to control these weeds was oxyfluorfen at 5kg prod./ha applied at pereemergence treatment on March 25, showing more than 90% control for 120 days after application. Preemergence application of oxyfluorfen at 3kg prod./ha on March 25 followed by paraquat 3kg prod./ha applied on June 25 as the foliar application can maintain the clean orchard for a year, showing on of the promising and applicable control system. The most appropriate rate and time for oxyfluorfen seemed to be 5kg prod./ha applied on March 25.

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Bearing Behavior Characteristics of Pressure Penetrating Steel Pipe Pile Under Compression Load (압축하중을 받는 압입강관말뚝의 지지거동 특성)

  • Kwon, Ohkyun
    • Journal of the Korean GEO-environmental Society
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    • v.16 no.7
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    • pp.5-13
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    • 2015
  • The pressure penetrating steel pipe pile method which can be constructed in a narrow space using the hydraulic jack is used on the foundation reinforcement, extension of the structure and basement, restoration of the differential settlement etc.. This method is possible to construct in narrow areas and low story height, the non-noise and non-vibration works, and it is possible for the construction site to be clean without slime. And it is possible to confirm the bearing capacity of pile due to penetrating the pile with the compression load of hydraulic jack. In this study, the static load test with the load-transfer test was carried out to investigate the bearing behavior characteristics of the pressure penetrating steel pipe pile. Four series of static load test were executed to investigate the variation of bearing behavior of the pressure penetrating steel pipe pile. As a result of these tests, the allowable load of the pressure penetrating steel pipe was evaluated more than 637 kN, and the shaft resistance corresponding to 81~86% of each applied load was mobilized with only a small portion of the base resistance acting. And it was also evaluated that the unit skin friction was mobilized to maximum value after two months.

Eco-Industrial Park (EIP) Development and Key Technologies for Clean Production (청정 생산을 위한 생태산업단지 구축과 주요기술)

  • Yoo, ChangKyoo;Heo, Soon-Ki;Yoo, Dong Joon;Lee, SeungJun;Shin, Ji Na;Park, Yong Joon;Yoon, Hack Mo;Chun, Hee Dong;Moon, Jeong Ki;Lee, In-Beum
    • Korean Chemical Engineering Research
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    • v.43 no.5
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    • pp.549-559
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    • 2005
  • Sustainable industrial development which can minimize an ecological effect by the mankind exertion is recently interested due to an environmental contamination and a resource exhaustion problem. An eco-industrial park (EIP) is a community of manufacturing and service businesses seeking enhanced environmental and economic performance through collaboration in managing environmental and resource issues, including energy, water, and materials. EIP developments which improve a production plant within an eco-friendly greenfield and design a new industrial ecosystem are accomplished recently, which can efficiently re-use the waste and resources from each company within EIP. In this review, the outside and domestic case studies of EIP and cornerstone technologies to develop the EIP, such as energy integration, waste reuse, mass flow analysis, water pinch, and life cycle assessment, are summarized.

A Study Regarding Measurements of Bacterial Contamination Levels in Radiology Room Equipment (방사선과 촬영실 장비의 세균오염도 측정)

  • Choi, Eun-Jin;Song, Hyeon-Je;Dong, Kyung-Rae;Kim, Chang-Bok;Ryu, Jae-Kwang;Kwak, Jong-Gil
    • Journal of Radiation Industry
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    • v.11 no.1
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    • pp.1-6
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    • 2017
  • Reported some level of bacteria in areas that are well made contact in Radiology imaging room evaluate the importance of cleanliness in the hospital management of equipment to check for the presence of pathogenic bacteria. Gwang-ju and Jeol-la city and medium-sized hospitals in the material with a cotton swab and rub evenly Radiology selection cassette, a handle, Apron of the imaging apparatus having the most contact with patients from July 2016 to August 2016 as a target in place and special studios 6, and saline solution will placed in a test tube containing. The swab sample was diluted 1,000 times, you can see the bacteria and the intestinal bacterial selective medium Trypticase Soy Agar (TSA), Muller-Hinton Agar (MHA), Eosin-Methylene Blue (EMB), ENDO(BD, NJ, USA) then incubated smear to. In the incubator (incubator, SANYO, Japan) was observed after incubation of bacteria and counting the total number of bacteria also Colonies (colony) suspected intestinal bacteria were isolated and cultured on KIA medium (BD, NJ, USA). As a result, it was found that this came Gram positive Coccus A hospital handle the F hospital, from the C Gram positive Coccus cassette and handle the F hospital. The striking yellow coloring Staphylococcus aureus 110 agar (STA 110) in the medium sample, but it is suspected staphylococcal Coccus to the final identification in the laboratory is not a single specimen of the two samples from Gram positive Coccus biochemical identification Identification Kit is an API could not, it was thought to be non-Staphylococcus aureus was cultured on blood agar suggesting that (BAP) blood of dance. Dynamic tests were conducted biochemical API kit of the two samples were identified from Gram positive Coccus bacteria Escherichia coli (E. coli) is F hospital cassette was confirmed Eenterobacter cloaca in A hospital possession. Did not aggregate O-26, O-111, O-157 and the serum test was conducted in the laboratory from the E. coli F cassette hospital.

Pesticide Multiresidues Analysis of Environmental-friendly Agricultural Soils by the Complex Cleanup Method of Accelerated Solvent Extraction (ASE) and Solid Phase Extraction (SPE) (ASE 및 SPE 복합정제법을 이용한 친환경농업토양의 다성분잔류농약 분석)

  • Moo, Kyung-Mi;Park, Jin-Woo;Lee, Young-Guen;Choi, Young-Whan
    • Journal of agriculture & life science
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    • v.45 no.5
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    • pp.73-80
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    • 2011
  • Fifty substances of pesticide were selected for analysis through the historical investigation of pesticides detected from environmental-friendly agricultural soil, and the environmental-friendly agricultural soils in Gyeongnam area were collected and then were accepted Anve (accelerated solvent extraction) and SPve (solid-phase extraction) as multiresidue extraction and clean up methods suitable to the soils. The pesticide residues were analyzed by using GC/vCD/NPD, HPLC/UV/FL, GC/MSD, or HPLC/MSD. 50 kinds of pesticides for the soils were an average of 95.5% from retrieval ratio of the 72 to 118% range, and the average of 3.0% for CV (%). Among 40 samples of soil, 20 components were detected from pesticide residues of 21 samples, and average amounts detected for these components were 0.035 for endosulfan, 0.043 for ethoprophos, 0.020 for chlorpyrifos, 0.023 for chlorfenapyr, 0.047 for flufenoxuron, 0.070 for fenvalerate, 0.266 for cypermethrin, 0.016 for lufenuron, 0.022 for bifenthrin, 0.025 for fenobucarb/BPMC, 0.043 for difenoconazole, 0.059 for fenarimol, 0.020 for kresoxim-methyl, 0.026 for tetraconazole, 0.039 for isoprothiolane, 0.017 for iprobenfos, 0.014 for nolrimol, 0.156 for fluquinconazole, 0.047 for tebuconazole, and 0.045 mg/kg for oxadiazon. Therefore it is infered that the establishment of pesticide residues limit for environmental-friendly agricultural soil is needed as soon as possible.