• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.3
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• pp.701-709
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• 2006

The Rhus verniciflua Stokes (乾漆-RVS) has been used in traditional East Asia medicine for the therapy of gastritis, stomach cancer, although the mechanism for the biological activity is unclear. In the present study aims to investigate RVS extract contributes to growth inhibitory effect and it's the molecular mechanism on the human gastric cancer cells. AGS (gastric cancer cells) and RIEI (normal cells) were treated to different concentrations and periods of RVS extract $(10{\;}{\sim{{\;}100{\;}ug/mil)$. Growth inhibitory effect was analyzed by measuring FACS study and MTS assay. Cell cycle inhibition was confirmed by measuring CDK2 kinase activity by immunoprecipitation and kinase assay. And apoptosis was confirmed by surveying caspase cascades activation using a pan caspase inhibitor Exposure to RVS extract (50 ug/mll) resulted in a synergistic inhibitory effect on cell growth in AGS cells. Growth inhibition was related with the inhibition of proliferation and induction of apoptosis. The extract induces Gl -cell cycle arrest through the regulation of cyclins, the induction of p27kip1, and the decrease CDK2 kinase activity. And upregulated p27kip1 level is caused by protein stability increment by the reduction of S-phase kinase-associated protein 2 (Skp2), a key molecule related with p27kip1 ubiquitination and degradation, and do novo protein synthesis. Besides, 乾漆 extract induces apoptosis through the expression of Bax, poly(ADP-ribose) polymerase (PARP) and activation of caspase-3. RVS extract induces Gl -cell cycle arrest via accumulation of p27kip1 and apoptosis in human gastric cancer cells but not in normal cells, therefore we suggest that the extract can be used as a novel class of anti-cancer drugs.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.495-509
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• 2016

Irrigation applications (IA) and increased bud load (BL) are fundamental practices that are used to achieve optimum yields in grape production, while maintaining fruit quality parameters. Two different irrigation amounts (IA-I and IA-II) based on growth stages, in addition to a non-irrigated (rain-fed) control, along with two different BL applications [normal bud load based on traditional pruning practices (1BL) and double bud load (2BL)] were evaluated over a two-year experiment for their effects on the biochemical composition of the berries. Berries from the rain-fed vines had higher sugar levels, whereas no significant change was detected in organic acid levels. The increased bud load (2BL) treatment yielded less sugar in the berries compared to the 1BL control in both years. The total non-colored phenolic compounds (NPC) were greater in the irrigated (especially IA-I) and 2BL treatments than in those of the rain-fed and 1BL control. However, total anthocyanin was greater in the non-irrigated and 1BL control than in the irrigated and 2BL treatments. The antioxidant contents of the berries also varied according to the treatments and years. Our results implied that implementing a higher bud load along with the IA-I irrigation application, in which irrigation applications were 50 and 75% of the cumulative evaporation from the Class A pan during berry set to veraison and veraison to harvest growth stages, respectively, can help in obtaining greater yields in high-plateau viticulture. Thus, if more buds are left on the vines, along with sufficient irrigation and rainfall, yield may increase while maintaining or increasing the biochemical composition of the berries.

• Journal of Korean Elementary Science Education
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• v.24 no.3
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• pp.242-248
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• 2005

The purpose of this study was to survey the actual condition about field learning materials in Gwangju and Jeonnam area. The questionnaire was developed to examine teachers' perceptions and actual condition of a field teaming related science subject at 145 elementary schools in Gwangju city and Jeonnam province. The results of this study were as follows. First, the rate of school in performing field teaming related to 'strata and fossils' in science class was $6.8\%$. Second, the place of dianosaur track fossils in Jeonnam province, which was suitable for field teaming 'strata and fossils: were well known by $75\%$ of teachers. Also $68\%$ of teachers have not visited those places. On the basis of this result, the followings are proposed. First, field teaming materials and place should be developed in each school district. Second, field teaming strategies should be developed in various level. Third, some programs to study how to teach in the field teaming and experience the various field places should be suggested to preservice teachers as well as inservice teachers.

• Journal of the Korean Society for Precision Engineering
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• v.32 no.6
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• pp.529-536
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• 2015

A defect could be generated in bolts for a use of oil filters for the manufacturing process and then may affect to the safety and quality in bolts. Also, fine defects may be imbedded in oil filter system. So it is very important that such defects be investigated and screened during the multiple manufacturing processes. Therefore, in order effectively to evaluate the fine defects, the FEM simulations were performed to make characterization in the crack detection of the bolts and the parameters such as number of turns of the coil, the coil size, applied frequency were calculated based on the simulation results. Simulations were carried out for the defect signal of eddy current probe. Exciter and receiver were utilized. In this paper, the FEM simulations were performed in both bobbin-type and pancake-type probe, both probes were optimized under Eddy current FEM simulations and the results of calculation were discussed.

• Journal of Gifted/Talented Education
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• v.26 no.4
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• pp.747-761
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• 2016

The purpose of this research is to study the perception of mathematical creativity through gifted elementary mathematics students. The analysis on perception for mathematical creativity was done by testing 200 elementary school students in grades 4, 5, and 6 who are receiving gifted education in elementary mathematics gifted class operated by ${\bigcirc}{\bigcirc}$ City Dept of Education through the questionnaire that was developed based on Rhodes' 4P theory. This survey asked them to name what they think is the most creative from educational programs they have as far received. Then we analyzed the reason for the students' choice of the creativity program and interviewed the teachers who had conducted chosen program. As a result of analyzing the data, these students chose as mathematical creativity primarily creative problem solving, task commitment, and interest in mathematics in such order. This result is explained through analyzing the questionnaire that was based on Rhodes' 4P theory on areas of process, product and press. The perception of mathematical creativity by the gifted mathematical students not only helps to clarify the concept of mathematical creativity but also has implication for future development for gifted education program.

• Journal of the Korea Convergence Society
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• v.12 no.5
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• pp.157-166
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• 2021

With the rising of the middle-class in China, the garage reserve has become an important element considered by the high-end community in improving user's experience. Taking Oak Bay, China as an example, this study aims to present a new system of storage service that can help companies achieve sustainable development of storage services. In order to achieve this goal, a customized service process is made by investigating user's need with sample visits and in-depth interviews, and finding user's key points with journey maps. The final analysis shows that this storage service system helps to expand the space and improve user's satisfaction. Therefore, this paper provides theoretical support and practical basis of garage services for other construction companies, and greatly improves the service system of community.

• Journal of the Korean Society for information Management
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• v.36 no.2
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• pp.57-77
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• 2019

Random Forest (RF), a representative ensemble technique, was applied to automatic classification of journal articles in the field of library and information science. Especially, I performed various experiments on the main factors such as tree number, feature selection, and learning set size in terms of classification performance that automatically assigns class labels to domestic journals. Through this, I explored ways to optimize the performance of random forests (RF) for imbalanced datasets in real environments. Consequently, for the automatic classification of domestic journal articles, Random Forest (RF) can be expected to have the best classification performance when using tree number interval 100~1000(C), small feature set (10%) based on chi-square statistic (CHI), and most learning sets (9-10 years).

• Korean Journal of Soil Science and Fertilizer
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• v.15 no.4
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• pp.213-220
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• 1982

Soil water changes in lysimeters with four different soils and two different available soil depths were monitored during the growing seasons of the soybean-barley cropping from 1977 to 1980 in Suweon to evaluate evapotranspiration (ET) as a function of available soil water and evaporative demand of the atmosphere. ET was calculated with soil water profile and water balance. Soil water content was measured with a neutron moisture depth gauage and The evaporative demand of the atmosphere was estimated with a class A pan evaporation. Rainfall. solar radiation, and wind speed were observed to examine heat and water balances. The average ET of soybeans ranged from 1.6 mm/day at seedling to 6.5 mm/day at flowering, and that of barley ranged from 0.5 mm/day at the regrowth stage to 4.6 mm/day at heading; however, a large variability was observed. The ratio of ET to pan evaporation ($ET/E_o$) ranged from 0.5 to 1.1 for soybeans and 0.4 to 1.2 for barley. The soil evaporation factor ($K_e$) of the $ET/E_o$ component decreased as the soil water depleted and the canopy developed. The crop transpiration factor ($K_t$), another component of $ET/E_o$, also was a function of time and the soil water. $K_t$ was constant when the available soil water fraction (f) in the root zone was greater than a threshold value, and $K_e$ was decreased linearly when f was lower than this threshold. The threshold was 0.7 for the moderate evaporative demand days, 0.4 to 0.5 for the low evaporative demand days, and 0.9 to 0.96 for the high evaporative demand days. Conclusively, the ET can be estimated from the evaporative demand of the atmosphere, $E_o$, $K_e$ and $K_t$, and the available soil water content in the root zone.

• Journal of Distribution Science
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• v.14 no.6
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• pp.45-52
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• 2016

Purpose - This study was conducted to present a study on the perception and satisfaction with the brand agricultural products targeted at consumers who use a lot of local products. According to the data of 2011, the total number of the brand agricultural products of Korea is 5,291 with various kinds. Research design, data and methodology - The survey shows that the brand agricultural products are being used by some specific people. However, it can be a useful idea which can help the consumption of brand agricultural products to be expanded if we understand how consumers' recognitions are different between various groups. For an empirical Analysis, the response data of 110 adult patients residing in the metropolitan area were used and conducted with a factor analysis, frequency analysis in order to ensure the validity and conducted a regression analysis and correlation analysis using SPSS statistical program. Results - According to the analysis, it showed consumers with an interest in brand agricultural products are 40-50 age housewives and the middle class of about 5 million won in monthly income more than 3 million won with a college education. As for consumers' purchasing status, all the subjects said that they had experienced buying brand agricultural products and the level of satisfaction for them was very high. Relatively, consumers' satisfaction level with high income and education is high. And recognition of the brand agricultural products was found mainly goes through word of mouth. The age and income are very important factors in customers' repurchase for brand agricultural products. The result of the analysis for the influences on brand agricultural products of customer satisfaction suggests even if the recognitions for safety, quality, and value are vital factors, the recognition of quality doesn't influence on brand agricultural products statistically and significantly. It was analysed if there were any differences between recognitions by group to brand agricultural products, that is to say recognition of safety, quality and value and the result can be summarized as follows. There are all statistical significant differences depending on their age, educational background and income. In the case of 30 or 40 aged, as they got the education level of college and graduate school and earned relatively high income, most customers have positive recognition on the brand agricultural products. This implies the group which can buy and consume the brand agricultural more easily has much more positive recognition. Conclusion - The results of this study shows consumers' brand awareness and satisfaction with brand agricultural products are affected by their age and income level. The purpose of this study is to find the information that can help brand agricultural products markets to be expanded by understanding the factors which encourage consumers to behave repurchase as well as customers' various levels of recognition to the brand agricultural products. The survey says that brand agricultural products are being used by some specific people.

• Biomolecules & Therapeutics
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• v.31 no.1
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• pp.73-81
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• 2023

Sirtuins (SIRTs) belong to the nicotinamide adenine dinucleotide (NAD+)-dependent class III histone deacetylase family. They are key regulators of cellular and physiological processes, such as cell survival, senescence, differentiation, DNA damage and stress response, cellular metabolism, and aging. SIRTs also influence carcinogenesis, making them potential targets for anticancer therapeutic strategies. In this study, we investigated the anticancer properties and underlying molecular mechanisms of a novel SIRT1 inhibitor, MHY2251, in human colorectal cancer (CRC) cells. MHY2251 reduced the viability of various human CRC cell lines, especially those with wild-type TP53. MHY2251 inhibited SIRT1 activity and SIRT1/2 protein expression, while promoting p53 acetylation, which is a target of SIRT1 in HCT116 cells. MHY2251 treatment triggered apoptosis in HCT116 cells. It increased the percentage of late apoptotic cells and the sub-G1 fraction (as detected by flow cytometric analysis) and induced DNA fragmentation. In addition, MHY2251 upregulated the expression of FasL and Fas, altered the ratio of Bax/Bcl-2, downregulated the levels of pro-caspase-8, -9, and -3 proteins, and induced subsequent poly(ADP-ribose) polymerase cleavage. The induction of apoptosis by MHY2251 was related to the activation of the caspase cascade, which was significantly attenuated by pre-treatment with Z-VAD-FMK, a pan-caspase inhibitor. Furthermore, MHY2251 stimulated the phosphorylation of c-Jun N-terminal kinase (JNK), and MHY2251-triggered apoptosis was blocked by pre-treatment with SP600125, a JNK inhibitor. This finding indicated the specific involvement of JNK in MHY2251-induced apoptosis. MHY2251 shows considerable potential as a therapeutic agent for targeting human CRC via the inhibition of SIRT1 and activation of JNK/p53 pathway.