• 전기학회논문지
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• 제56권8호
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• pp.1450-1454
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• 2007

This paper presents a microfabricated Clark-type sensor which exactly can measure dissolved oxygen in the cell containing solution. We designed, fabricated, and characterized a microfabircated Clark-type oxygen sensor for measuring dissolved oxygen. The microfabricated oxygen sensor consists of 3-electrodes on a glass substrate, a FEP (Fluorinated ethylene propylene) oxygen-permeable membrane, and PDMS (Polydimethylsiloxane) reservoir for storing sample solution. Thin-film Ag/AgCl was employed as a reference electrode and its durability was verified by obtaining a stable open circuit potential for 2 hours against a commercial Ag/AgCl electrode and a stable cyclic voltammetry curve. Selectivity, response time, and linearity of the fabricated oxygen sensor were also verified well by cyclic voltammetry and amperometry depending. The fabricated oxygen sensor showed a 90% response time of 40sec and an excellent linearity with a correlation coefficient of 0.994.

• E2M - 전기 전자와 첨단 소재
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• 제8권3호
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• pp.254-260
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• 1995

A method is described for the design and fabrication of the sensor interface circuits on the Clark electrodes for the dissolved oxygen(DO). The discussion includes a method for the +5 V single-supply driving for the sensor circuits, which has low power comsumption for the front-end electronics. DO probe under test is composed of the Clark electrode with silver anode, gold cathode and the electrolyte of half saturated KCI solution and the FEP teflon memtrance for the oxygen penetration. Typical polarograms for the DO probes by using this sensor circuit reveals high accuracy over 99% of the I to V conversion. Partial pressure of oxygen obtained from the polarograms are well suited to the results calculated. It is expected that the proposed sensor circuits can be utilized into the customized IC for the battery-driven small-size DO meters.

• 센서학회지
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• 제19권1호
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• pp.36-42
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• 2010

This paper describes a multi channel measurement system which can measure the cellular respiration level in a solution containing cells by using a Clark-type sensor with the solution temperature control unit. The Clark-type sensor can measure the cellular respiration level in the solution because it can measure the reduction current depending on the dissolved oxygen level in the solution. This measurement system was maintained the temperature within ${\pm}0.1^{\circ}C$ of the setting temperature value by on/off control method in order to measure the precise cellular respiration level. The measurement system showed that the applied voltage to the working electrode was very stable(-0.8 V$\pm$ 0.0071 V) by using proportional control method. From the current measurement, the response time and the linearity correlation coefficient were 25 sec and 0.94, respectively, which are very close to the results of the commercial product. Using this system and the fabricated Clarktype sensor, the average ratio of the uncoupled OCR(oxygen consumption rate) to the coupled OCR was 1.35 and this is almost the same as that obtained from a commercial systems.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 제38회 하계학술대회
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• pp.276-277
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• 2007

본 논문에서는 미량의 세포를 포함한 용액 내에서 세포의 산소호흡량을 측정하기 위해 FEP(Fluorinated Ethylene Propylene)를 멤브레인으로 사용한 Clark-type 센서를 제안하였다. 제안된 Clark-type 센서는 3-전극 시스템을 구성하는 유리 기판, 산소를 선택적으로 투과 시키는 FEP 멤브레인과 세포를 담을 수 있는 PDMS reservoir로 구성된다. 산소 센서의 3-전극 시스템에서 작업 전극과 상대 전극으로는 Au, 기준 전극으로는 Ag/AgCl을 사용하였다. 기준 전극은 Ag 전극을 0.1M KCl/Tris-HCl 용액에서 chlorination하여 표면에 AgCl이 형성되도록 하였고, OCP(Open Circuit Potential) test를 수행한 결과 2시간 동안 안정적인 OCP 특성을 보여 좋은 내구성을 가짐을 확인하였다. 또한, 산소 유무에 따른 cyclic voltammetry 그래프의 차이를 확인하고, amperometry로 감도 및 반응 시간, 선형성을 측정/분석하였다. 제작된 산소 센서는 40초의 90% 반응 시간과 0.994의 아주 좋은 선형 상관계수를 보여주었다.

• 센서학회지
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• 제10권3호
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• pp.180-186
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• 2001

최근 전류 법적인 Clark형 센서의 단점을 극복하기 위해 FET형 용존 산소 센서가 제안되었다. 그러나 제안된 센서의 출력은 작업전극에서 발생하는 pH의 변화를 감지하기 때문에 유속에 대한 영향을 받는다. 본 논문에서는 FIA(flow injection analysis)를 이용하여 유속의 영향을 최소화 할 수 있는 방향을 결정하였다. 그리고 완충막을 작업전극과 감지막 위에 형성시켜 작업전극과 감지부가 측정용액에 직접 노출되어 용액의 유동에 의해 센서의 출력이 불안정한 문제를 개선하였다. TEOS(tetraethylorthosilicate)와 DEDMS(diethoxydimethylsilane)를 혼합하여 졸-겔법으로 완충막을 형성하였다.

• KSBB Journal
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• 제13권1호
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• pp.52-57
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• 1998

The present work proposes a simple electrochemical method applicable to any immobilization processes of oxidase using a Clark type oxygen electrode as a base transducer. The present work suggests an optimal immobilization technique among three different methods of glucose oxidase(GOD) onto one side of $37[\mu}$mthick blend membranes, composed o 80% of cellulose triacetate and 20% of polycaprolactone, on the basis of the maximum Michaelis-Menten parameter(Vm) determined by either steady state or transient analyses. The electrode system was made of disk type gold cathode(4mm diameter) and Ag/AgCl anode. One side of the blend membrane was in contact with the cathode surface while the other side was immobilized with GOD either in covalent-bond or cross-linked forms, the latter being covered by $25{\mu}$m thick dialysis membrane of cellulose acetate. The resultant current density was on-line monitored by a potentiostat while glucose level was varied from 1 to 20 mM. The present study shows that direct cross-linking of GOD with glutaraldehyde was mostly preferred for fabrication of glucose sensor, on the basis of resultant kinetic parameters from either steady state or transient analyses.

• Nuclear Engineering and Technology
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• 제55권6호
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• pp.2195-2205
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• 2023

One of the salient nuclear fuel performance parameters for new fuel types under development is changes in fuel thickness. To test the new commercially fabricated U-10Mo monolithic plate-type fuel, an irradiation experiment was designed that consisted of multiple mini-plate capsules distributed within the Advanced Test Reactor (ATR) core, the mini-plate 1 (MP-1) experiment. Each capsule contains eight mini-plates that were either fueled or "dummy" plates. Fuel thickness changes within a fuel assembly can be characterized by measuring the gaps between the plates ultrasonically. The channel gap probe (CGP) system is designed to measure the gaps between the plates and will provide information that supports qualification of U-10Mo monolithic fuel. This study will discuss the design and the results from the use of a custom-designed CGP system for characterizing the gaps between mini-plates within the MP-1 capsules. To ensure accurate and repeatable data, acceptance and calibration procedures have been developed. Unfortunately, there is no "gold" standard measurement to compare to CGP measurements. An effort was made to use plate thickness obtained from post-irradiation measurements to derive channel gap estimates for comparison with the CGP characterization.