Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Development of a multi channel measurement system for the cellular respiration measurement

세포 호흡량 측정용 다채널 측정 시스템 개발

  • Nam, Hyun-Wook (School of Electrical Engineering, Korea University) ;
  • Park, Jung-Il (School of Electrical Engineering, Korea University) ;
  • KimPak, Young-Mi (Department of Physiology, College of Medicine, Department of Nanopharmaceutical and Life Sciences, Kyung Hee University) ;
  • Pak, James Jung-Ho (School of Electrical Engineering, Korea University)
  • 남현욱 (고려대학교 전기전자전파공학과) ;
  • 박정일 (고려대학교 전기전자전파공학과) ;
  • 김영미 (경희대학교 의과대학 생리학교실, 나노의약생명과학과) ;
  • 박정호 (고려대학교 전기전자전파공학과)
  • Published : 2010.01.30
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Abstract

This paper describes a multi channel measurement system which can measure the cellular respiration level in a solution containing cells by using a Clark-type sensor with the solution temperature control unit. The Clark-type sensor can measure the cellular respiration level in the solution because it can measure the reduction current depending on the dissolved oxygen level in the solution. This measurement system was maintained the temperature within ${\pm}0.1^{\circ}C$ of the setting temperature value by on/off control method in order to measure the precise cellular respiration level. The measurement system showed that the applied voltage to the working electrode was very stable(-0.8 V$\pm$ 0.0071 V) by using proportional control method. From the current measurement, the response time and the linearity correlation coefficient were 25 sec and 0.94, respectively, which are very close to the results of the commercial product. Using this system and the fabricated Clarktype sensor, the average ratio of the uncoupled OCR(oxygen consumption rate) to the coupled OCR was 1.35 and this is almost the same as that obtained from a commercial systems.

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References

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