• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.2
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• pp.143-148
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• 1998

This study was carried out in order to investigate oxidative deterioration during dehydration at $40^{\circ}C,\;50^{\circ}C$ and $60^{\circ}C$ of sea mussel and baby clam. Moisture content was decreased with drying temperature and time. Sea mussel was dehydrated more rapidly than baby clam that had Harder muscle tissue. Both samples were not reached to Aw 0.62 in case of 10 hrs drying at $40^{\circ}C$, But it reached within 8 hrs in sea mussel and 10 hrs in baby clam at $50^{\circ}C$, respectively. Even if $60^{\circ}C$ could speed up drying, it caused to form more free fatty acid, peroxide, thiobarbituric acid and brown pigments. Lipophilic brown pigment was 10 times higher than hydrophilic and actively increased in all samples. fluorescence intensity was also increased with drying temperature and time. Particularly, it was higher sea mussel than baby clam more or less.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.3
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• pp.227-234
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• 1985

The present study was carried out for making clear the organic acids composition of some shellfishes and their boiled-dried products. Three species of shellfish such as top shell, hard clam and abalone were chosen as the experimental samples and analysed for their nonvolatile organic acids composition by gas liquid chromatography (GLC). Eight kinds of nonvolatile organic acid were identified in raw and boiled-dried top shell, major ones were succinic, malic and pyroglutamic acid. Out of nine kinds of nonvolatile acid was determined in raw and boiled-dried of hard clam, abundant ones were succinic, lactic and malic acid, And seven kinds of nonvolatile organic acid were detected in boiled-dried abalone while eight kinds in raw sample, abundant ones were succinic, lactic and pyroglutamic acid, respectively. In all samples, the total amount of nonvolatile organic acid was the most abundant in hard clam while the least in abalone, and the content of succinic acid was appeared as the most abundant one. In dried samples, the total amount of the nonvolatile organic acid was determined to be about half-decrease after the boiled-dried treatment of the raw samples, of which abalone showed the highest decreasing을 rate whereas hard clam was the lowest. In general, the decreasing rate of each acid was the higher in fumaric, malic and citric acid than in maleic, oxalic and succinic acid.

• Journal of Aquaculture
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• v.11 no.2
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• pp.183-191
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• 1998

To substitute fromzed and freeze-dried food for live food in the development of the artificial seedling production of bivalve larvae, the dietary value of live T. suecica was compared with those of freeze-dried T. suecica, frozed T. suecica, live T. suecica (50%)+frozen T. suecica (50%), and live T. suecica (50%)+freeze-dried T. suecica (50%) in the rearing of oyster (Crassotrea gigas) and hen clam (Mactra chinensis) larvae, and manila clam (Tapes philipninarum) spats. Oyster larvae fed live T. suecica showed the highest growth (shell hight $231.9^{\mu}$m) and survival rate (72.6%) and those fed freeze-dried T. suecica showed the lowest growth (shell height $168.9^{\mu}$m) and survival rate (35.3%). However, in the hen clam larvae, there were not significantly different among diet group in growth and survival rate. The small spats of manila clam fed live t. suecica or live T. suecica (50%)+freeze-dried T. suecica (50%) showed higher growth and survival rate than those fed other diet group. In the case of large spats of manila clam, live T. suecica and live T. suecica (50%)+frozen T. suecica (50%) showed better growht. But, survival rates were not different among diet groups. Dietary valuse of frozen and dried T. suecica were different on species and growth stage, and frozen and freeze-dried T. suecica can be partially used as substitute food for T. suecica live T. succica in shellfish hatchery.

• Journal of Aquaculture
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• v.17 no.2
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• pp.115-121
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• 2004

In a series of feeding experiments using the newly established the first laboratory strain of a temperate photosynthetic ciliate, Mesodinium rubrum MR-MAL01, direct evidence was obtained for ingestion of the cultured M. rubrum cells by the bivalves like the Korean scallop, Chlamys farreri and Manila clam. Ruditapes phil-ippinarum. Each experimental Korean scallop and Manila clam removed up to 9,590 and 23,200 cells min-1, respectively. Small particles almost identical to the ruptured cells of MR-MAL01 culture were observed in the gut of the experimental bivalves, although no intact M. rubrum cell was found. In a 28 days rearing experiment, Isoshrysis galbana (KMCC H-2), a microalga, supported better growth of small Manila clam spat (0.46 mm in shell length) than MR-MAL01 strain. For the large Manila clam spat (0.84 mm in shell length), however. MR-MAL01 strain was a better prey item. Mass culture methods for this photosynthetic marine ciliate may be developed for use as live feed in aquaculture of the spat and broodstock of bivalve and small-mouthed fish larvae.

• Journal of Aquaculture
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• v.9 no.4
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• pp.419-427
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• 1996

In order to obtain the basic information for seedling production of surf clam, Spisula sachalinensis, spawning induction and egg development were investigated. $NH_4OH$ addition and serotonin injection could induce the spawning in surf clam. Water temperature rising, sperm suspension immersion, UV-ray irradiated seawater and $H_2O_2$ addition less affected on induction of spawning than $NH_4OH$ and serotonin did. On the other hand, males were more sensitive to the treatments than females. The response time to initial spawning in the case of $NH_4OH$ addition was $3\~4$ hours. However in the case of serotonin injection, it was within 5 minutes. The number of eggs released by $NH_4OH$ addition were significantly more than those released by serotonin injection. The serotonin injection induced higher rates of germinal vesicle breakdown than the $NH_4OH$ addition. Fertilizing and hatching rates of the eggs also were the similar results. Eggs of surf clam were demersal isolated eggs and averaging $65.2{\pm}±1.8\;{\mu}m$ in diameter after spawning. Optimum range of water temperature for the development of egg was $15\~20^{\circ}C$, The required time for development of D-shaped larvae was 42 hours at $15^{\circ}C$ and 27 hours at $20^{\circ}C$, respectively.

• Korean Journal of Environmental Biology
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• v.22 no.1
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• pp.28-34
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• 2004

The feeding, clearance rate, and ingestion rate of Manila clam (Ruditapes philippinarum) were investigated with small group (10.8$\pm$.58 mm in shell length) and large group (37.5$\pm$3.8 mm in shell length) under combination in water temperature (24, 27, 33, $36^{\circ}C$) With salinity (12, 15, 24, 27$\textperthousand$). The maximum feeding, clearance rate, and ingestion rate in small and large groups were $27^{\circ}C$ and 27$\textperthousand$, respectively. While, The minimum feeding, clearance rate, and ingestion rate in both groups were $36^{\circ}C$ and 12$\textperthousand$, respectively. There results were suggested that the feeding, clearance rate, and ingestion rate of Manila clam were significantly influenced by external factors such as water temperature and salinity. The reason for the mass mortality of Manila clam during the heavy rainfall in summer season can be explained by high temperature and low salinity.

• Journal of fish pathology
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• v.11 no.2
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• pp.143-150
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• 1998

Characteristics and distribution of the natural commensal flora in the surrounding environment and tissues of clam in Hadong area were studied under varying conditions of growth media and incubation temperatures. Total numbers of bacteria present in intestinal tract, gill, body fluid and surrounding mud were found to be not influenced by the used BHIA, STA and SNA media. Although the growth rate of bacteria at the condition of $15^{\circ}C$ incubation temperature was slower than that of $25^{\circ}C$ and $35^{\circ}C$, it showed the highest number of total bacteria compared with other two different conditions of incubation temperature. Interestingly, the proportion of bacteria able to form colony on several selective media was higher in replica analysis from nutrient media to selective media than that in direct smearing from samples. The generic diversity of bacteria isolated from the tissues and analyzed by API 20E and API 20NE kit showed similar pattern with each other and distinct from that of environment. The distribution of bacteria in the surrounding mud or mantle fluid of clam indicated a high diversity comparable to that found for the gill or intestinal tract microflora, with Pseudomonas being the prevalent group. It implies that the tissues of clam may probide a selective habitat for a commensal microflora.

• Journal of Aquaculture
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• v.15 no.2
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• pp.111-117
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• 2002

Effect of substrate composition, stocking density and food organisms on survival and growth of the surf clam, Spisula sachalinensis was studied. Spat collection from the substratum was the highest, when its sand size was less than 250 $\mu m4; at such substratum, survival and growth of the surf clam were also the highest. The spat (1.4 mm) grew to 4.6 mm and 8.2 mm in shell length on day 30 and 50, respectively. The relationship between shell length (SL) and shell height (SH), shell breadth (SB), total weight (TW) and shell weight (SW) of the spat are formulated by the following equations; SH : 0.7351SL+0.2272 ( $r^2$ =0.9600)i SB = 0.4037SL+0.2112 ($r^2$ = 0.9452); TW : 0.2824SL^{2.7020} ($r^2$ = 0.9820)i SW = $0.4021SL^{2.0217} ($r^2$ : 0.6169). In the substratum containing fine sand, growth and survival of the clam roared at the density of 1~2 ind./$cm^2$ were optimal. The quality of feed decreases in the following order; C. calcitrans, mixture group, I. galbana, P. lutheri, T. suecica.

• Korean Journal of Food Science and Technology
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• v.14 no.1
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• pp.6-10
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• 1982

To investigate the effect of organic acids on suppression of fishy odor, the change of the odor and the remaining amount of trimethyamine (TMA), selected as the representative component of fishy odor in clam pickle (15.5% NaCl) were examined by sensory evaluation and gas chromatography. The fishy odor linearly decreased as the amount of vinegar increased at ${\alpha}=0.01$, but there was no significant difference of fishness score for the changes of storage period and kinds of acid. Overall acceptability score was found to be the best when 8 ml vinegar added to 100g clam pickle. The remaining amount of TMA showed the tendency to decrease as the amount of acetic acid and storage time increased and kinds of acid were changed in order of acetic, malic and citric acid. However when $4\;{\mu}l/ml$ acetic acid (=8 ml vinegar/100g clam pickle) was added, the percentage of the remaining amount of TMA showed little decrease from about 95% for 72 hours of storage time. From the results of sensory evaluation and gas chromatography, it was considered that acids have the effect on suppression of fishy odor by producing non-volatile salts by combining with TMA chemically and that the effect of acids on suppression of fishy odor is mainly due to the masking effect at the concentration commonly used.

• The Korean Journal of Malacology
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• v.23 no.1
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• pp.31-38
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• 2007

Population ecological parameters and stock biomass of the purplish washington clam, Saxidomus purpuratus (Sowerby), in the southern coastal areas of Korea were determined based on the fishery data from the Divers Fisheries Cooperative and other available biological data. Instantaneous coefficient of total mortality (Z) of purplish Washington clam was estimated to be 0.7479/year. The estimated instantaneous coefficient of natural mortality (M) was 0.2012/year. From the values of Z and M, the instantaneous coefficient of fishing mortality coefficient (F) for the recent years was calculated to be 0.4578/year. The age of purplish Washington clam at its first capture ($t_c$) was 2.7506 year. Yield-per-recruit and spawning biomass-per-recruit were estimated under harvest strategies that bases on $F_{max}$, $F_{0.1}$, $F_{35%}$ and $F_{40%}$ were shown as 81.60 g, 61.68 g, 115.07 g and 131.51 g, respectively. The acceptable biological catch (ABC) was estimated to be about 1,404 metric tons.