• Molecules and Cells
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• 제26권3호
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• pp.319-322
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• 2008

Little is known about the chromosomal variability and polymorphism existing in mitotic chromosomes of Citrus, mainly due to lack of reliable chromosomal markers and small chromosome size. To test the hypothesis of chromosomal polymorphism and provide the foundation of the genome organization in the Citrus cultivars, we have developed molecular cytogenetic markers for 13 Citrus species collected from Jeju island, Korea. In this study, we demonstrated that the chromosomal locations of cytogenetic markers are quite variable and extremely polymorphic, in contrast to the previous studies. The data obtained in this study will be of utmost importance in cytological systematics and karyotyping of the Citrus species.

• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.529-535
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• 2007

The nutritive values of leaves of Citrus grandis, Citrus aurantium, Citrus oranges, Citrus limon, and Citrus deliciosa were evaluated by chemical composition and in vitro gas production techniques. There were significant (p<0.001) differences among citrus species in terms of chemical composition. Crude protein (CP) contents ranged from 123.0 to 148.3 g/kg DM. Neutral detergent fibre (NDF) and acid detergent fibre (ADF) contents were varied with species in the range 219.4-355.4 and 215.0-278.8 g/kg DM respectively. Condensed tannin (CT) contents were ranged from 5.9 to 10.2 g/kg DM. The PEG addition significantly (p<0.001) increased the gas production and some estimated parameters of citrus tree leaves. However, species showed variable responses to polyethylene glycol (PEG) treatment. There were also significant (p<0.001) differences among species in terms of gas production and estimated parameters. The OMD and ME contents of citrus leaves without PEG supplementation were ranged from 66.5 to 73.3% and 9.8 to 10.9 MJ/kg DM respectively. The improvement in gas production, organic matter digestibility (OMD) and metabolizable energy (ME) with PEG emphasized the negative effect of tannins on digestibility. The increase (%) in the estimated OMD and ME contents ranged from 5.5 to 9.8% and 5.7 to 10.2% respectively. All citrus tree leaves studied in this experiment have potential nutritive values indicated by high crude protein content, OMD, ME and low fiber values.

• Journal of Applied Biological Chemistry
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• 제44권3호
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• pp.143-146
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• 2001

Eight flavonoids, including rutin, naringin, hesperidin, quercetin, hesperetin, nobiletin, 3,5,6,7,8,3',4'-methoxylated flavone, and tangeretin, in the peels of satsuma mandarin (Citrus unshiu) species of Halla, Gungcheon, Hungjin, Namgam-20, Illnam-1, and Chungdo harvested between August and December were analyzed through HPLC. Hesperidin content of Halla harvested during early maturation was 28.70 mg/g, and was the highest among the tested citrus fruits. Rutin content of Hungjin harvested during early maturation was 2.66 mg/g. Naringin in all citrus species and hesperetin in Halla, Gungchun, Namgam-20, and Chungdo were only detected in the peel of fruits harvested during early maturation. Hesperidin and rutin were detected mainly in all citrus species, and other flavonoids in trace. Flavonoid content in the peel of fruits was high during early maturation. Flavonoid contents in the peels of all fruit samples were generally high in the early stage of maturation, which then decreased rapidly.

• Mycobiology
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• 제49권3호
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• pp.267-274
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• 2021

Two Diaporthe species isolated from fruit of Citrus sinensis in China were characterized based on morphology and multilocus phylogeny of ITS, tef1, and tub2 gene sequences. The phylogeny indicated that the two species match Diaporthe taoicola and D. siamensis. A critical examination of phenotypic characteristics confirmed the phylogenetic results. Diaporthe taoicola was morphologically characterized by producing Alpha conidia with tapering toward both ends. Meanwhile, D. siamensis produced cylindrical or ellipsoidal Alpha conidia with two oil drops. Pathogenicity tests revealed that both species were pathogenic to fruit of C. sinensis. To our knowledge, the two species were firstly reported on Citrus sinensis in China.

• 한국응용곤충학회지
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• 제39권4호
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• pp.267-274
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• 2000

1996년부터 1998년까지 제주도 감귤원에 발생하여 피해를 주는 해충들을 조사란 결과 곤충류 69종과 그 밖의 해충으로 5종이 조사되었는데, 그 중 귤응애(Panonychus citri), 귤굴나방Phyllocnistis citrella), 조팝나무진딧물(Aphis citricola), 목화진딧물(Aphis gossypii), 꽃노랑총채벌레 (Frankliniella occidentalis)등이 감귤에 큰 피해를 주는 우점종들이었다. 특히 꽃노랑총채벌레와 뒷흰날개밤나방(Peridroma saucia)은 최근 들어 국내에 유입된 해충으로 그 피해가 증가하고 있다. 감귤의 잎, 가지, 과실, 꽃을 가해하는 해충의 수는 각각 36종, 16종, 41종, 2종이었으며, 이중 과실을 가해하는 해충에 의한 피해과율은 1997년과 1998년에 각각 20.5%와 18.6%로 약 20%정도의 피해를 받고 있으며, 그 중 비상품과율은 각각 6.4%와 6.7%였다.

• 한국응용곤충학회지
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• 제9권1호
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• pp.33-35
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• 1970

The author collected 2 species of spider mites from persimmon and citrus trees in Taegu and Chun Chon during the summer of 1961 and 1969. Tenuipalpus japonicus collected from persimmon trees and Panonychus citri collected from citrus trees in and from trifoliate orange trees. In 1958 and 1961 Dr. E. S. Lee recorded four phytophagous species, i.e. Panonychus ulmi, Tetranychus pacificus, Tetranychus urticae and Bryobia praetiosa.

• 생명과학회지
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• 제21권10호
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• pp.1452-1459
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• 2011

귤나무속은 운향과(Rutaceae)에 속한다. 동남아시아, 미얀마, 중국 운남에서 기원된 것으로 알려져 있다. 이 속의 분류관계는 복잡한데 클론번식과 야생식물과 교잡이 빈번하다. 식물에서 속간 속내 계통관계 추론을 위해 널리 이용되고 있는 엽록체 trnL-trnF 부위가 있다. 이 귤나무속과 두 근연한 탱자나무속과 금감속에 속하는 7종간 계통 관계를 평가하였다. 많은 삽입이 발견되었고 속내 종간 변이는 결실/삽입에 의한 것으로 밝혀졌다. 이 속의 레몬과 당귤나무은 네 계통도(MP, ML, ME, and NJ)에서 모두 같은 분지군을 형성하여 가장 근연관계에 있었고 광귤나무(향귤나무)과 자매군을 형성하였다. 유자나무는 이들과 많은 서열 차이를 나타내었다. 외부 분지군은 낮은 지지도를 나타내었다.

• 한국응용곤충학회지
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• 제17권1호
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• pp.7-13
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• 1978

감귤해충 종합방제 확립을 위한 기초자료를 얻고저 제주도 서귀포에서 1973-1976년까지 4년간 실시한 결과 다음과 같은 결과를 얻었다. 1. 귤응애는 7철 중순에서 8철 중순 사이에 11회의 큰 peak를 나타냈다. 2. 한편 관행살포구에서는 7,8월뿐 아니라 9-11월에도 높은 밀도를 나타냈다. 3. 귤응애의 천적으로 Oligota yasumatsui K., Anysits baccarum L., Hemerobiid sp. Semidalis albate E., Orius sp., Agistemus terminalis Q., 무당벌레류 3종 포식응애 1종등 10종이 발견되었다. 4. 귤응애의 년도별 발생경향을 보며 무살포구에서는 매년 감소하는 경향이고 관행살포구에서는 계속 증가하는 경향이었다. 5. 살균제로서 석회볼드액과 동수화제 처리구는 귤응애밀도가 높았으나 항생제인 Streptomycin은 낮은밀도였다.

• Food Science and Biotechnology
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• 제15권3호
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• pp.357-362
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• 2006

We assessed various antioxidant activities, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis 3-ethylbenz-thiazoline-6-sulfonic acid (ABTS) radical, and reactive oxygen species (ROS) radical scavenging effect, along with antioxidant capacity, of soybean oil with added Citrus species peel extracts (CPEs). These extract oils showed higher radical scavenging effects than grape fruit seed extract, the natural antioxidant agent, did. When CPEs were added to soybean oil, they showed peroxide value (POV) and acid value (AV) increasing inhibition effects. Furthermore, none of the CPEs showed any cytotoxicity over the tested concentration range of 0.01-100 ppm. The major flavonoid contents of Citrus junos, as determined by HPLC, were naringin ($7.5\;{\mu}g/mg$) and neohesperidin ($7.5\;{\mu}g/mg$), and those of Citrus unshiu were narirutin ($3.13\;{\mu}g/mg$) and hesperidin ($1.97\;{\mu}g/mg$). However, the aglycone form was not found. This study showed that CPEs might be a potent source of natural antioxidant, without any toxic effects.

• 한국자원식물학회지
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• 제10권3호
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• pp.213-220
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• 1997

The present study has been undertaken to characterize availability of citrus as a medicinal plant with antineoplastic property. The crude extracts from 40 species of fruits with 12 species of the local Citrus in Cheju island were evaluated on their potential activities against mouse P388 lymphocytic leukaemia in vitro. The percent cytotoxicity varied from 25.40 to 97.94% at a concentration of $100{\mu}g/mL$. Among 40 spp., 8 species showed high toxicity more than 90% against P388 cells and Cheongkyool(C. nippokoreana) exhibited the most cytotoxicity as 97.94%($IC_{50}=20.2{\mu}g/mL$). Nine varieties of C. junos were showed insiginicant cytotoxicity. In trifoliate orange, immature fruit was stronger than mature and peel extract showed higher cytotoxicity($IC_{50}=18{\mu}g/mL$) than the other tissues. Hexane fraction from methanol(MeOH) extract of trifoliate orange showed highly significant inhibition of cell growth($IC_{50}=3.9{\mu}g/mL$). In addition, its cytotoxicity increased remarkably from 3.95 to $0.40{\mu}g/mL$ as exposure time legthened. Cytotoxic activities of crude extracts were decreased considerably during a six months storage period. It was apparent that there is considerable variation in cytotoxicity, depending upon species, maturity and storage time of extracts. There was no meaningful cytotoxic difference between archicitrus and metacitrus in the genus Citrus.